Nicholas T. Southern
YOU?
Author Swipe
View article: Phage-Assisted Evolution of Allosteric Protein Switches
Phage-Assisted Evolution of Allosteric Protein Switches Open
Allostery, the transmission of locally induced conformational changes to distant functional sites, is a key mechanism for protein regulation 1 . Artificial allosteric effectors enable remote manipulation of cell function 2,3 ; their engine…
View article: Modular Engineering of Thermo-Responsive Allosteric Proteins
Modular Engineering of Thermo-Responsive Allosteric Proteins Open
Thermogenetics enables non-invasive spatiotemporal control over protein activity in living cells and tissues, yet its applications have largely been restricted to transcriptional regulation and membrane recruitment. Here, we present a gene…
View article: Rational engineering of allosteric protein switches by<i>in silico</i>prediction of domain insertion sites
Rational engineering of allosteric protein switches by<i>in silico</i>prediction of domain insertion sites Open
Domain insertion engineering is a powerful approach to juxtapose otherwise separate biological functions, resulting in proteins with new-to-nature activities. A prominent example are switchable protein variants, created by receptor domain …
View article: An Improved CRISPR/dCas9 Interference Tool for Neuronal Gene Suppression
An Improved CRISPR/dCas9 Interference Tool for Neuronal Gene Suppression Open
The expression of genetic material governs brain development, differentiation, and function, and targeted manipulation of gene expression is required to understand contributions of gene function to health and disease states. Although recen…
View article: An improved CRISPR/dCas9 interference tool for neuronal gene suppression
An improved CRISPR/dCas9 interference tool for neuronal gene suppression Open
The expression of genetic material governs brain development, differentiation, and function, and targeted manipulation of gene expression is required to understand contributions of gene function to health and disease states. Although recen…