Nicky Scheefhals
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View article: <i>SCN1A</i> -deficient excitatory neuronal networks display mutation-specific phenotypes
<i>SCN1A</i> -deficient excitatory neuronal networks display mutation-specific phenotypes Open
Dravet syndrome is a severe epileptic encephalopathy, characterized by (febrile) seizures, behavioural problems and developmental delay. Eighty per cent of patients with Dravet syndrome have a mutation in SCN1A, encoding Nav1.1. Milder cli…
View article: Living on the edge
Living on the edge Open
The brain is a tremendously complex organ that consists of an intricate network of billions of nerve cells, also called neurons. Neurons are the main communicators of the brain and by sending signals from one neuron to the other they can t…
View article: ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons
ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons Open
The correct subcellular distribution of proteins establishes the complex morphology and function of neurons. Fluorescence microscopy techniques are invaluable to investigate subcellular protein distribution, but they suffer from the limite…
View article: ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons
ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons Open
The correct subcellular distribution of protein complexes establishes the complex morphology of neurons and is fundamental to their functioning. Thus, determining the dynamic distribution of proteins is essential to understand neuronal pro…