Nicola L. B. Pohl
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View article: Recent advances in the synthesis of extensive libraries of heparan sulfate oligosaccharides for structure–activity relationship studies
Recent advances in the synthesis of extensive libraries of heparan sulfate oligosaccharides for structure–activity relationship studies Open
View article: Efficient platform for synthesizing comprehensive heparan sulfate oligosaccharide libraries for decoding glycosaminoglycan–protein interactions
Efficient platform for synthesizing comprehensive heparan sulfate oligosaccharide libraries for decoding glycosaminoglycan–protein interactions Open
View article: Automated Platform for the Synthesis of Heparan Sulfate Oligosaccharide Libraries for Decoding Glycosaminoglycan-Protein Interactions
Automated Platform for the Synthesis of Heparan Sulfate Oligosaccharide Libraries for Decoding Glycosaminoglycan-Protein Interactions Open
Glycosaminoglycans (GAGs) are abundant, ubiquitous carbohydrates in biology, yet their structural complexity has limited an understanding of their biological roles and structure-function relationships. Synthetic access to large collections…
View article: Fully automated fast-flow synthesis of antisense phosphorodiamidate morpholino oligomers
Fully automated fast-flow synthesis of antisense phosphorodiamidate morpholino oligomers Open
Rapid development of antisense therapies can enable on-demand responses to new viral pathogens and make personalized medicine for genetic diseases practical. Antisense phosphorodiamidate morpholino oligomers (PMOs) are promising candidates…
View article: Automated, Multistep Continuous‐Flow Synthesis of 2,6‐Dideoxy and 3‐Amino‐2,3,6‐trideoxy Monosaccharide Building Blocks
Automated, Multistep Continuous‐Flow Synthesis of 2,6‐Dideoxy and 3‐Amino‐2,3,6‐trideoxy Monosaccharide Building Blocks Open
An automated continuous flow system capable of producing protected deoxy‐sugar donors from commercial material is described. Four 2,6‐dideoxy and two 3‐amino‐2,3,6‐trideoxy sugars with orthogonal protecting groups were synthesized in 11–32…
View article: Fully automated fast-flow synthesis of antisense phosphorodiamidate morpholino oligomers
Fully automated fast-flow synthesis of antisense phosphorodiamidate morpholino oligomers Open
View article: Structural insight into the binding of human galectins to corneal keratan sulfate, its desulfated form and related saccharides
Structural insight into the binding of human galectins to corneal keratan sulfate, its desulfated form and related saccharides Open
View article: Automated Fast-Flow Synthesis of Antisense Phosphorodiamidate Morpholino Oligomers
Automated Fast-Flow Synthesis of Antisense Phosphorodiamidate Morpholino Oligomers Open
The antisense phosphorodiamidate morpholino oligomer (PMO) drugs Eteplirsen and Golodirsen are improving the lives of some Duchenne muscular dystrophy (DMD) patients, but treating all DMD subtypes would require the development of over 50 n…
View article: Automated Fast-Flow Synthesis of Antisense Phosphorodiamidate Morpholino Oligomers
Automated Fast-Flow Synthesis of Antisense Phosphorodiamidate Morpholino Oligomers Open
The antisense phosphorodiamidate morpholino oligomer (PMO) drugs Eteplirsen and Golodirsen are improving the lives of some Duchenne muscular dystrophy (DMD) patients, but treating all DMD subtypes would require the development of over 50 n…
View article: Parallel Glyco-SPOT Synthesis of Glycopeptide Libraries
Parallel Glyco-SPOT Synthesis of Glycopeptide Libraries Open
View article: Automated Solution-Phase Synthesis of <i>S</i>-Glycosides for the Production of Oligomannopyranoside Derivatives
Automated Solution-Phase Synthesis of <i>S</i>-Glycosides for the Production of Oligomannopyranoside Derivatives Open
Thioglycosides are more resistant to enzymatic hydrolysis than their O-linked counterparts, thereby becoming attractive targets for carbohydrate-based therapeutic development. We report the first development of methods for the site-selecti…
View article: Modular continuous flow synthesis of orthogonally protected 6-deoxy glucose glycals
Modular continuous flow synthesis of orthogonally protected 6-deoxy glucose glycals Open
The use of a continuous flow platform for the rapid and highly efficient construction of differentially protected glycals from commercial sources is described.
View article: Automated solution-phase syntheses of alpha 1 → 2, 1 → 3 type rhamnans and rhamnan sulfate fragments
Automated solution-phase syntheses of alpha 1 → 2, 1 → 3 type rhamnans and rhamnan sulfate fragments Open
View article: Advancing Solutions to the Carbohydrate Sequencing Challenge
Advancing Solutions to the Carbohydrate Sequencing Challenge Open
Carbohydrates possess a variety of distinct features with stereochemistry playing a particularly important role in distinguishing their structure and function. Monosaccharide building blocks are defined by a high density of chiral centers.…
View article: Acid-mediated <i>N</i>-iodosuccinimide-based thioglycoside activation for the automated solution-phase synthesis of α-1,2-linked-rhamnopyranosides
Acid-mediated <i>N</i>-iodosuccinimide-based thioglycoside activation for the automated solution-phase synthesis of α-1,2-linked-rhamnopyranosides Open
Carbohydrate structures are often complex. Unfortunately, synthesis of the range of sugar combinations precludes the use of a single coupling protocol or set of reagents. Adapting known, reliable bench-chemistry reactions to work via autom…
View article: Putting sugars under strain
Putting sugars under strain Open
Adding strain to sugars enables synthesis of natural products and unusual oligosaccharides
View article: Design and synthesis of multivalent α-1,2-trimannose-linked bioerodible microparticles for applications in immune response studies of <i>Leishmania major</i> infection
Design and synthesis of multivalent α-1,2-trimannose-linked bioerodible microparticles for applications in immune response studies of <i>Leishmania major</i> infection Open
Leishmaniasis, a neglected tropical disease, currently infects approximately 12 million people worldwide with 1 to 2 million new cases each year in predominately underdeveloped countries. The treatment of the disease is severely underdevel…
View article: Introduction: Carbohydrate Chemistry
Introduction: Carbohydrate Chemistry Open
ADVERTISEMENT RETURN TO ISSUEEditorialNEXTIntroduction: Carbohydrate ChemistryNicola L. B. Pohl*Nicola L. B. PohlIndiana University Bloomington*E-mail: [email protected]More by Nicola L. B. PohlView Biographyhttp://orcid.org/0000-0001-7747…
View article: Synthesis of protected glucose derivatives from levoglucosan by development of common carbohydrate protecting group reactions under continuous flow conditions
Synthesis of protected glucose derivatives from levoglucosan by development of common carbohydrate protecting group reactions under continuous flow conditions Open
View article: Scope and limitations of carbohydrate hydrolysis for de novo glycan sequencing using a hydrogen peroxide/metallopeptide-based glycosidase mimetic
Scope and limitations of carbohydrate hydrolysis for de novo glycan sequencing using a hydrogen peroxide/metallopeptide-based glycosidase mimetic Open
View article: Leishmania-Derived Trimannose Modulates the Inflammatory Response To Significantly Reduce Leishmania major-Induced Lesions
Leishmania-Derived Trimannose Modulates the Inflammatory Response To Significantly Reduce Leishmania major-Induced Lesions Open
Leishmania lipophosphoglycan (LPG) is a key virulence factor, initiating inflammation resulting in cutaneous lesions. LPG is capped by various oligosaccharides. How these glycans are recognized and how they alter the course of Leishmania i…
View article: A High‐Throughput Mass‐Spectrometry‐Based Assay for Identifying the Biochemical Functions of Putative Glycosidases
A High‐Throughput Mass‐Spectrometry‐Based Assay for Identifying the Biochemical Functions of Putative Glycosidases Open
The most commonly employed glycosidase assays rely on bulky ultraviolet or fluorescent tags at the anomeric position in potential carbohydrate substrates, thereby limiting the utility of these assays for broad substrate characterization. H…
View article: Effects of varying the 6-position oxidation state of hexopyranoses: a systematic comparative computational analysis of 48 monosaccharide stereoisomers
Effects of varying the 6-position oxidation state of hexopyranoses: a systematic comparative computational analysis of 48 monosaccharide stereoisomers Open
View article: Recent liquid chromatographic approaches and developments for the separation and purification of carbohydrates
Recent liquid chromatographic approaches and developments for the separation and purification of carbohydrates Open
This review highlights current techniques for carbohydrate purification and identifies research gaps.
View article: Recent Advances in the Analysis of Complex Glycoproteins
Recent Advances in the Analysis of Complex Glycoproteins Open
ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTRecent Advances in the Analysis of Complex GlycoproteinsStefan Gaunitz†, Gabe Nagy†, Nicola L. B. Pohl†, and Milos V. Novotny*†‡View Author Information† Department of Chemistry, Indiana University…
View article: Overcoming the limited availability of human milk oligosaccharides: challenges and opportunities for research and application
Overcoming the limited availability of human milk oligosaccharides: challenges and opportunities for research and application Open
Human milk oligosaccharides (HMOs) are complex sugars highly abundant in human milk but currently not present in infant formula. Rapidly accumulating evidence from in vitro and in vivo studies, combined with epidemiological associations an…
View article: Designing sugar mimetics: non-natural pyranosides as innovative chemical tools
Designing sugar mimetics: non-natural pyranosides as innovative chemical tools Open
View article: Protocol for the purification of protected carbohydrates: toward coupling automated synthesis to alternate-pump recycling high-performance liquid chromatography
Protocol for the purification of protected carbohydrates: toward coupling automated synthesis to alternate-pump recycling high-performance liquid chromatography Open
Analytical techniques that can be coupled to automated oligosaccharide synthesis platforms are needed to purify to homogeneity protected carbohydrates at levels of ≥99.5% purity.
View article: Automated Solution-Phase Synthesis of Insect Glycans to Probe the Binding Affinity of Pea Enation Mosaic Virus
Automated Solution-Phase Synthesis of Insect Glycans to Probe the Binding Affinity of Pea Enation Mosaic Virus Open
Pea enation mosaic virus (PEMV)--a plant RNA virus transmitted exclusively by aphids--causes disease in multiple food crops. However, the aphid-virus interactions required for disease transmission are poorly understood. For virus transmiss…
View article: Automated Solution-Phase Synthesis of β-1,4-Mannuronate and β-1,4-Mannan
Automated Solution-Phase Synthesis of β-1,4-Mannuronate and β-1,4-Mannan Open
The first automated solution-phase synthesis of β-1,4-mannuronate and β-1,4-mannan oligomers has been accomplished by using a β-directing C-5 carboxylate strategy. By utilizing fluorous-tag assisting purification after repeated reaction cy…