Nicolas Rouhier
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View article: Fusion of a bacterial cysteine desulfurase to redox-sensitive green fluorescent protein produces a highly sensitive cysteine biosensor for monitoring changes in intracellular cysteine
Fusion of a bacterial cysteine desulfurase to redox-sensitive green fluorescent protein produces a highly sensitive cysteine biosensor for monitoring changes in intracellular cysteine Open
Over the last two decades, the development of fluorescent probes has transformed the way of measuring physiological parameters in intact cells, including in the field of redox biology. We developed a genetically encoded biosensor called Cy…
View article: Novel atypical thioredoxins with potential redox activity in eukaryote photosynthetic organisms
Novel atypical thioredoxins with potential redox activity in eukaryote photosynthetic organisms Open
Photosynthetic organisms have a high diversity of proteins belonging to the thioredoxin (TRX) superfamily. It comprises more than 150 proteins distributed in different families and classes, including thioredoxins, glutaredoxins, protein di…
View article: Participation of a cysteine tetrad in the recycling mechanism of methionine sulfoxide reductase A from radiation-tolerant Deinococcus bacteria
Participation of a cysteine tetrad in the recycling mechanism of methionine sulfoxide reductase A from radiation-tolerant Deinococcus bacteria Open
Methionine oxidation leads to the formation of methionine sulfoxide (MetO), which is reduced back to Met by methionine sulfoxide reductases (Msrs). The catalytic mechanism used by A-type Msr (MsrA) for MetO reduction requires a catalytic c…
View article: Ferredoxin NADP+ reductase for NADPH and NADH regeneration in a flow bioelectrochemical reactor
Ferredoxin NADP+ reductase for NADPH and NADH regeneration in a flow bioelectrochemical reactor Open
Ferredoxin-NADP+ reductase (FNR) is an efficient and selective biocatalyst to continuously regenerate the NADPH cofactor consumed in biomolecular synthesis for the chemical and pharmaceutical sectors. In this work, FNR from Chla…
View article: Dual Roles of Reducing Systems in Protein Persulfidation and Depersulfidation
Dual Roles of Reducing Systems in Protein Persulfidation and Depersulfidation Open
The oxidative modification of specific cysteine residues to persulfides is thought to be the main way by which hydrogen sulfide (H2S) exerts its biological and signaling functions. Therefore, protein persulfidation represents an important …
View article: Iron‑sulfur cluster synthesis in plastids by the SUF system: A mechanistic and structural perspective
Iron‑sulfur cluster synthesis in plastids by the SUF system: A mechanistic and structural perspective Open
View article: Correction of non-random mutational biases along a linear bacterial chromosome by the mismatch repair endonuclease NucS
Correction of non-random mutational biases along a linear bacterial chromosome by the mismatch repair endonuclease NucS Open
The linear chromosome of Streptomyces exhibits a highly compartmentalized structure with a conserved central region flanked by variable arms. As double strand break (DSB) repair mechanisms play a crucial role in shaping the genome plastici…
View article: INTEGRATION OF THE IRON-SULFUR CLUSTER TRANSFER PROTEIN NFU1 OF THE GREEN MICROALGA CHLAMYDOMONAS IN CHLOROPLAST METABOLISM
INTEGRATION OF THE IRON-SULFUR CLUSTER TRANSFER PROTEIN NFU1 OF THE GREEN MICROALGA CHLAMYDOMONAS IN CHLOROPLAST METABOLISM Open
International audience
View article: Correction of non-random mutational biases along a linear bacterial chromosome by the mismatch repair endonuclease NucS
Correction of non-random mutational biases along a linear bacterial chromosome by the mismatch repair endonuclease NucS Open
The linear chromosome of Streptomyces exhibits a highly compartmentalized structure with a conserved central region flanked by variable arms. As double strand break (DSB) repair mechanisms play a crucial role in shaping the genome plastici…
View article: Oxidation of Arabidopsis thaliana COX19 Using the Combined Action of ERV1 and Glutathione
Oxidation of Arabidopsis thaliana COX19 Using the Combined Action of ERV1 and Glutathione Open
Protein import and oxidative folding within the intermembrane space (IMS) of mitochondria relies on the MIA40–ERV1 couple. The MIA40 oxidoreductase usually performs substrate recognition and oxidation and is then regenerated by the FAD-dep…
View article: Ascorbate Peroxidase 2 (APX2) of Chlamydomonas Binds Copper and Modulates the Copper Insertion into Plastocyanin
Ascorbate Peroxidase 2 (APX2) of Chlamydomonas Binds Copper and Modulates the Copper Insertion into Plastocyanin Open
Recent phylogenetic studies have unveiled a novel class of ascorbate peroxidases called “ascorbate peroxidase-related” (APX-R). These enzymes, found in green photosynthetic eukaryotes, lack the amino acids necessary for ascorbate binding. …
View article: Mitochondrial ferredoxin-like is essential for forming complex I-containing supercomplexes in Arabidopsis
Mitochondrial ferredoxin-like is essential for forming complex I-containing supercomplexes in Arabidopsis Open
In eukaryotes, mitochondrial ATP is mainly produced by the oxidative phosphorylation (OXPHOS) system, which is composed of 5 multiprotein complexes (complexes I–V). Analyses of the OXPHOS system by native gel electrophoresis have revealed …
View article: Relationships between the Reversible Oxidation of the Single Cysteine Residue and the Physiological Function of the Mitochondrial Glutaredoxin S15 from Arabidopsis thaliana
Relationships between the Reversible Oxidation of the Single Cysteine Residue and the Physiological Function of the Mitochondrial Glutaredoxin S15 from Arabidopsis thaliana Open
Glutaredoxins (GRXs) are widespread proteins catalyzing deglutathionylation or glutathionylation reactions or serving for iron-sulfur (Fe-S) protein maturation. Previous studies highlighted a role of the Arabidopsis thaliana mitochondrial …
View article: The mitochondrial ferredoxin-like is essential for the formation of complex I-containing respiratory supercomplexes in <i>Arabidopsis thaliana</i>
The mitochondrial ferredoxin-like is essential for the formation of complex I-containing respiratory supercomplexes in <i>Arabidopsis thaliana</i> Open
In eukaryotes, mitochondrial ATP is mainly produced by the oxidative phosphorylation (OXPHOS) system, which is composed of five multiprotein complexes (complexes I to V). Analyses of the OXPHOS system by native gel electrophoresis revealed…
View article: Redox regulation of enzymes involved in sulfate assimilation and in the synthesis of sulfur-containing amino acids and glutathione in plants
Redox regulation of enzymes involved in sulfate assimilation and in the synthesis of sulfur-containing amino acids and glutathione in plants Open
Sulfur is essential in plants because of its presence in numerous molecules including the two amino acids, cysteine, and methionine. Cysteine serves also for the synthesis of glutathione and provides sulfur to many other molecules includin…
View article: Biochemical and Structural Insights on the Poplar Tau Glutathione Transferase GSTU19 and 20 Paralogs Binding Flavonoids
Biochemical and Structural Insights on the Poplar Tau Glutathione Transferase GSTU19 and 20 Paralogs Binding Flavonoids Open
Glutathione transferases (GSTs) constitute a widespread superfamily of enzymes notably involved in xenobiotic detoxification and/or in specialized metabolism. Populus trichocarpa genome (V4.1 assembly, Phytozome 13) consists of 74 genes co…
View article: Maturation and Assembly of Iron-Sulfur Cluster-Containing Subunits in the Mitochondrial Complex I From Plants
Maturation and Assembly of Iron-Sulfur Cluster-Containing Subunits in the Mitochondrial Complex I From Plants Open
In plants, the mitochondrial complex I is the protein complex encompassing the largest number of iron-sulfur (Fe-S) clusters. The whole, membrane-embedded, holo-complex is assembled stepwise from assembly intermediates. The Q and N modules…
View article: Thiol Reductases in Deinococcus Bacteria and Roles in Stress Tolerance
Thiol Reductases in Deinococcus Bacteria and Roles in Stress Tolerance Open
Deinococcus species possess remarkable tolerance to extreme environmental conditions that generate oxidative damage to macromolecules. Among enzymes fulfilling key functions in metabolism regulation and stress responses, thiol reductases (…
View article: The cytosolic Arabidopsis thaliana cysteine desulfurase ABA3 delivers sulfur to the sulfurtransferase STR18
The cytosolic Arabidopsis thaliana cysteine desulfurase ABA3 delivers sulfur to the sulfurtransferase STR18 Open
View article: Structural Insights into a Fusion Protein between a Glutaredoxin-like and a Ferredoxin-Disulfide Reductase Domain from an Extremophile Bacterium
Structural Insights into a Fusion Protein between a Glutaredoxin-like and a Ferredoxin-Disulfide Reductase Domain from an Extremophile Bacterium Open
In eukaryotic photosynthetic organisms, ferredoxin–thioredoxin reductases (FTRs) are key proteins reducing several types of chloroplastic thioredoxins (TRXs) in light conditions. The electron cascade necessary to reduce oxidized TRXs invol…
View article: A Redox-Sensitive Cysteine Is Required for PIN1At Function
A Redox-Sensitive Cysteine Is Required for PIN1At Function Open
Parvulins are ubiquitous peptidyl-prolyl isomerases (PPIases) required for protein folding and regulation. Among parvulin members, Arabidopsis PIN1At, human PIN1, and yeast ESS1 share a conserved cysteine residue but differ by the presence…
View article: Protein lipoylation in mitochondria requires Fe–S cluster assembly factors NFU4 and NFU5
Protein lipoylation in mitochondria requires Fe–S cluster assembly factors NFU4 and NFU5 Open
Plants have evolutionarily conserved NifU (NFU)-domain proteins that are targeted to plastids or mitochondria. “Plastid-type” NFU1, NFU2, and NFU3 in Arabidopsis (Arabidopsis thaliana) play a role in iron–sulfur (Fe–S) cluster assembly in …
View article: The Fe-S cluster assembly factors NFU4 and NFU5 are primarily required for protein lipoylation in mitochondria
The Fe-S cluster assembly factors NFU4 and NFU5 are primarily required for protein lipoylation in mitochondria Open
Plants have evolutionarily conserved NFU-domain proteins that are targeted to plastids or mitochondria. The ‘plastid-type’ NFU1, NFU2 and NFU3 in Arabidopsis thaliana play a role in iron-sulfur (Fe-S) cluster assembly in this organelle, wh…
View article: Atypical Iron-Sulfur Cluster Binding, Redox Activity and Structural Properties of Chlamydomonas reinhardtii Glutaredoxin 2
Atypical Iron-Sulfur Cluster Binding, Redox Activity and Structural Properties of Chlamydomonas reinhardtii Glutaredoxin 2 Open
Glutaredoxins (GRXs) are thioredoxin superfamily members exhibiting thiol-disulfide oxidoreductase activity and/or iron-sulfur (Fe-S) cluster binding capacities. These properties are determined by specific structural factors. In this study…
View article: Occurrence, Evolution and Specificities of Iron-Sulfur Proteins and Maturation Factors in Chloroplasts from Algae
Occurrence, Evolution and Specificities of Iron-Sulfur Proteins and Maturation Factors in Chloroplasts from Algae Open
Iron-containing proteins, including iron-sulfur (Fe-S) proteins, are essential for numerous electron transfer and metabolic reactions. They are present in most subcellular compartments. In plastids, in addition to sustaining the linear and…
View article: Gene atlas of iron‐containing proteins in <i>Arabidopsis thaliana</i>
Gene atlas of iron‐containing proteins in <i>Arabidopsis thaliana</i> Open
SUMMARY Iron (Fe) is an essential element for the development and physiology of plants, owing to its presence in numerous proteins involved in central biological processes. Here, we established an exhaustive, manually curated inventory of …
View article: Integration of the iron-sulfur transfer protein NFU1 of the microalga C. reinhardtii in chloroplast metabolism in the light, in the dark and in anaerobiosis
Integration of the iron-sulfur transfer protein NFU1 of the microalga C. reinhardtii in chloroplast metabolism in the light, in the dark and in anaerobiosis Open
International audience
View article: Arabidopsis thaliana 3-mercaptopyruvate sulfurtransferases interact with and are protected by reducing systems
Arabidopsis thaliana 3-mercaptopyruvate sulfurtransferases interact with and are protected by reducing systems Open
View article: Role of the chloroplastic NFU3 iron-sulfur transfer protein in the microalga Chlamydomonas reinhardtii
Role of the chloroplastic NFU3 iron-sulfur transfer protein in the microalga Chlamydomonas reinhardtii Open
International audience
View article: The Arabidopsis Mitochondrial Glutaredoxin GRXS15 Provides [2Fe-2S] Clusters for ISCA-Mediated [4Fe-4S] Cluster Maturation
The Arabidopsis Mitochondrial Glutaredoxin GRXS15 Provides [2Fe-2S] Clusters for ISCA-Mediated [4Fe-4S] Cluster Maturation Open
Iron-sulfur (Fe-S) proteins are crucial for many cellular functions, particularly those involving electron transfer and metabolic reactions. An essential monothiol glutaredoxin GRXS15 plays a key role in the maturation of plant mitochondri…