Nicole M. Wakida
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View article: Polarized microscopy with picrosirius red: potential for reproducible burn depth grading
Polarized microscopy with picrosirius red: potential for reproducible burn depth grading Open
This research explores the challenges in accurately classifying burn severity and the limitations associated with traditional histology-based model training. Histology, often considered the gold standard for determining burn wound severity…
View article: Exploring astrocyte morphological changes under shear stress: a quantum imaging and laser-induced shockwaves approach
Exploring astrocyte morphological changes under shear stress: a quantum imaging and laser-induced shockwaves approach Open
View article: Investigation of astrocytes’ morphological changes in response to laser-induced shockwave
Investigation of astrocytes’ morphological changes in response to laser-induced shockwave Open
Traumatic Brain Injury (TBI) arises from an external force affecting the brain, leading to a range of outcomes from mild to severe. Despite continuous scientific advancements, it continues to pose a persistent threat and remains a signific…
View article: Diminished LC3-Associated Phagocytosis by Huntington’s Disease Striatal Astrocytes
Diminished LC3-Associated Phagocytosis by Huntington’s Disease Striatal Astrocytes Open
Background: In recent years the functions of astrocytes have shifted from conventional supportive roles to also include active roles in altering synapses and engulfment of cellular debris. Recent studies have implicated astrocytes in both …
View article: Combining quantitative phase microscopy and laser-induced shockwave for the study of cell injury
Combining quantitative phase microscopy and laser-induced shockwave for the study of cell injury Open
In this paper, we propose a new system for studying cellular injury. The system is a biophotonic work station that can generate Laser-Induced Shockwave (LIS) in the cell culture medium combined with a Quantitative Phase Microscope (QPM), e…
View article: Laser-Induced Nuclear Damage Signaling and Communication in Astrocyte Networks Through Parp-Dependent Calcium Oscillations
Laser-Induced Nuclear Damage Signaling and Communication in Astrocyte Networks Through Parp-Dependent Calcium Oscillations Open
Astrocytes are known to respond to various perturbations with oscillations of calcium, including to cellular injury. Less is known about astrocytes’ ability to detect DNA/nuclear damage. This study looks at changes in calcium signaling in …
View article: Application of Laser Microirradiation in the Investigations of Cellular Responses to DNA Damage
Application of Laser Microirradiation in the Investigations of Cellular Responses to DNA Damage Open
Since the laser has been invented it has been highly instrumental in ablating different parts of the cell to test their functionality. Through induction of damage in a defined sub-micron region in the cell nucleus, laser microirradiation t…
View article: Fluid Shear Stress Enhances the Phagocytic Response of Astrocytes
Fluid Shear Stress Enhances the Phagocytic Response of Astrocytes Open
Astrocytes respond to brain injury at a cellular level by the process of reactive astrogliosis, and are able to adjust their response according to the severity of the insult. Included in the reactive response is the process of phagocytosis…
View article: Calcium Dynamics in Astrocytes During Cell Injury
Calcium Dynamics in Astrocytes During Cell Injury Open
The changes in intracellular calcium concentration ([Ca2+]) following laser-induced cell injury in nearby cells were studied in primary mouse astrocytes selectively expressing the Ca2+ sensitive GFAP-Cre Salsa6f fluor…
View article: Simulating Traumatic Brain Injury (TBI) using laser-induced shockwave under quantitative phase microscopy
Simulating Traumatic Brain Injury (TBI) using laser-induced shockwave under quantitative phase microscopy Open
Traumatic brain injury (TBI) occurs when an external shock causes injury to the brain. The mechanism of the disease is not completely understood yet. Studies have shown that astrocytes play various roles following brain injury. However, th…
View article: Astrocyte Phagocytosis Under Shear Flow
Astrocyte Phagocytosis Under Shear Flow Open
Phagocytic response of astrocytes in response to shear flow
View article: Nuclear Damage Calcium Oscillations
Nuclear Damage Calcium Oscillations Open
Calcium signaling in response to nuclear damage
View article: Phagocytic response of astrocytes to damaged neighboring cells
Phagocytic response of astrocytes to damaged neighboring cells Open
This study aims to understand the phagocytic response of astrocytes to the injury of neurons or other astrocytes at the single cell level. Laser nanosurgery was used to damage individual cells in both primary mouse cortical astrocytes and …
View article: Laser Microirradiation to Study <em>In Vivo</em> Cellular Responses to Simple and Complex DNA Damage
Laser Microirradiation to Study <em>In Vivo</em> Cellular Responses to Simple and Complex DNA Damage Open
DNA damage induces specific signaling and repair responses in the cell, which is critical for protection of genome integrity. Laser microirradiation became a valuable experimental tool to investigate the DNA damage response (DDR) in vivo. …