Nikhil T Awatade
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View article: Inflammation‐induced loss of <scp>CFTR</scp>‐expressing airway ionocytes in non‐eosinophilic asthma
Inflammation‐induced loss of <span>CFTR</span>‐expressing airway ionocytes in non‐eosinophilic asthma Open
Background and Objective Severe asthma is a heterogeneous disease with subtype classification according to dominant airway infiltrates, including eosinophilic (Type 2 high), or non‐eosinophilic asthma. Non‐eosinophilic asthma is further di…
View article: Comparison of commercially available differentiation media on cell morphology, function, and anti-viral responses in conditionally reprogrammed human bronchial epithelial cells
Comparison of commercially available differentiation media on cell morphology, function, and anti-viral responses in conditionally reprogrammed human bronchial epithelial cells Open
Primary air liquid interface (ALI) cultures of bronchial epithelial cells are used extensively to model airway responses. A recent advance is the development of conditional reprogramming that enhances proliferative capability. Several diff…
View article: The Complex Association between COPD and COVID-19
The Complex Association between COPD and COVID-19 Open
Chronic obstructive pulmonary disease (COPD) is significant cause of morbidity and mortality worldwide. There is mounting evidence suggesting that COPD patients are at increased risk of severe COVID-19 outcomes; however, it remains unclear…
View article: Comparison of Commercially Available Differentiation Media on Cell Morphology, Function, and Anti-Viral Responses in Conditionally Reprogrammed Human Bronchial Epithelial Cells
Comparison of Commercially Available Differentiation Media on Cell Morphology, Function, and Anti-Viral Responses in Conditionally Reprogrammed Human Bronchial Epithelial Cells Open
Introduction: Primary air liquid interface (ALI) cultures of bronchial epithelial cells are used extensively to model airway responses. A recent advance is the development of conditional reprogramming that enhances proliferative capability…
View article: A Comparative Study of Differentiation Media on Conditionally Reprogrammed Human Bronchial Epithelial Cells: Morphology, Function, and Host-Anti viral responses
A Comparative Study of Differentiation Media on Conditionally Reprogrammed Human Bronchial Epithelial Cells: Morphology, Function, and Host-Anti viral responses Open
Supplementary figure 1
View article: A Comparative Study of Differentiation Media on Conditionally Reprogrammed Human Bronchial Epithelial Cells: Morphology, Function, and Host-Anti viral responses
A Comparative Study of Differentiation Media on Conditionally Reprogrammed Human Bronchial Epithelial Cells: Morphology, Function, and Host-Anti viral responses Open
Table S2. Components of Lonza ALI base differentiation media
View article: A Comparative Study of Differentiation Media on Conditionally Reprogrammed Human Bronchial Epithelial Cells: Morphology, Function, and Host-Anti viral responses
A Comparative Study of Differentiation Media on Conditionally Reprogrammed Human Bronchial Epithelial Cells: Morphology, Function, and Host-Anti viral responses Open
<p><strong>Table S6. Short-circuit currents and electrophysiologic parameters in ALI base differentiation media. </strong>Data represent short circuit current values for baseline currents and resistance of monolayers…
View article: A Comparative Study of Differentiation Media on Conditionally Reprogrammed Human Bronchial Epithelial Cells: Morphology, Function, and Host-Anti viral responses
A Comparative Study of Differentiation Media on Conditionally Reprogrammed Human Bronchial Epithelial Cells: Morphology, Function, and Host-Anti viral responses Open
Table S7. Short-circuit currents and electrophysiologic parameters in PneumaCult differentiation media. Data represent short circuit current values for baseline currents and resistance of monolayers. Amiloride inhibited EN…
View article: A Comparative Study of Differentiation Media on Conditionally Reprogrammed Human Bronchial Epithelial Cells: Morphology, Function, and Host-Anti viral responses
A Comparative Study of Differentiation Media on Conditionally Reprogrammed Human Bronchial Epithelial Cells: Morphology, Function, and Host-Anti viral responses Open
Table S4. Pharmacologic compounds used for Ussing chamber measurements
View article: A Comparative Study of Differentiation Media on Conditionally Reprogrammed Human Bronchial Epithelial Cells: Morphology, Function, and Host-Anti viral responses
A Comparative Study of Differentiation Media on Conditionally Reprogrammed Human Bronchial Epithelial Cells: Morphology, Function, and Host-Anti viral responses Open
Table S3. Components of StemCell media expansion and differentiation
View article: A Comparative Study of Differentiation Media on Conditionally Reprogrammed Human Bronchial Epithelial Cells: Morphology, Function, and Host-Anti viral responses
A Comparative Study of Differentiation Media on Conditionally Reprogrammed Human Bronchial Epithelial Cells: Morphology, Function, and Host-Anti viral responses Open
Table S5. Genes assessed by PCRTable S5. Genes assessed by PCR
View article: A Comparative Study of Differentiation Media on Conditionally Reprogrammed Human Bronchial Epithelial Cells: Morphology, Function, and Host-Anti viral responses
A Comparative Study of Differentiation Media on Conditionally Reprogrammed Human Bronchial Epithelial Cells: Morphology, Function, and Host-Anti viral responses Open
Supplementary tables: Table S1. Components of CR cell expansion media
View article: Comparison of commercially available differentiation media on morphology, function, and virus-host interaction in conditionally reprogrammed human bronchial epithelial cells
Comparison of commercially available differentiation media on morphology, function, and virus-host interaction in conditionally reprogrammed human bronchial epithelial cells Open
Introduction Primary air liquid interface (ALI) cultures of bronchial epithelial cells are used extensively to model airway responses. A recent advance is the development of conditional reprogramming that enhances proliferative capability.…
View article: Type 2 Immunity and Its Impact on COVID-19 Infection in the Airways
Type 2 Immunity and Its Impact on COVID-19 Infection in the Airways Open
Type 2 immune responses are characterized by elevated type 2 cytokines and blood eosinophilia. Emerging evidence suggests that people with chronic type 2 inflammatory lung diseases are not particularly susceptible to SARS-CoV-2 infection. …
View article: Molecular dynamics and functional characterization of I37R-CFTR lasso mutation provide insights into channel gating activity
Molecular dynamics and functional characterization of I37R-CFTR lasso mutation provide insights into channel gating activity Open
Characterization of I37R, a mutation located in the lasso motif of the CFTR chloride channel, was conducted by theratyping several CFTR modulators from both potentiator and corrector classes. Intestinal current measurements in rectal biops…
View article: <i>In silico</i>and functional characterisation of an ultra-rare<i>CFTR</i>mutation identifies novel lasso motif interactions regulating channel gating
<i>In silico</i>and functional characterisation of an ultra-rare<i>CFTR</i>mutation identifies novel lasso motif interactions regulating channel gating Open
Characterisation of I37R – a novel mutation in the lasso motif of ABC-transporter CFTR, a chloride channel – was conducted by theratyping using CFTR potentiators which increase channel gating activity and correctors which repair protein tr…
View article: Molecular dynamics and theratyping in airway and gut organoids reveal R352Q-CFTR conductance defect
Molecular dynamics and theratyping in airway and gut organoids reveal R352Q-CFTR conductance defect Open
A significant challenge to making targeted CFTR modulator therapies accessible to all individuals with cystic fibrosis (CF) are many mutations in the CFTR gene that can cause CF, most of which remain uncharacterized. Here, we characterized…
View article: Significant functional differences in differentiated Conditionally Reprogrammed (CRC)- and Feeder-free Dual SMAD inhibited-expanded human nasal epithelial cells
Significant functional differences in differentiated Conditionally Reprogrammed (CRC)- and Feeder-free Dual SMAD inhibited-expanded human nasal epithelial cells Open
View article: An open-source high-content analysis workflow for CFTR function measurements using the forskolin-induced swelling assay
An open-source high-content analysis workflow for CFTR function measurements using the forskolin-induced swelling assay Open
Motivation The forskolin-induced swelling (FIS) assay has become the preferential assay to predict the efficacy of approved and investigational CFTR-modulating drugs for individuals with cystic fibrosis (CF). Currently, no standardized qua…
View article: Novel Antioxidant Therapy with the Immediate Precursor to Glutathione, γ-Glutamylcysteine (GGC), Ameliorates LPS-Induced Cellular Stress in In Vitro 3D-Differentiated Airway Model from Primary Cystic Fibrosis Human Bronchial Cells
Novel Antioxidant Therapy with the Immediate Precursor to Glutathione, γ-Glutamylcysteine (GGC), Ameliorates LPS-Induced Cellular Stress in In Vitro 3D-Differentiated Airway Model from Primary Cystic Fibrosis Human Bronchial Cells Open
Systemic glutathione deficiency, inflammation, and oxidative stress are hallmarks of cystic fibrosis (CF), an inherited disease that causes persistent lung infections and severe damage to the respiratory system and many of the body organs.…
View article: Significant functional differences despite morphological and molecular similarity in fully differentiated matched Conditionally Reprogrammed (CRC) and Feeder free dual SMAD inhibited expanded human nasal epithelial cells
Significant functional differences despite morphological and molecular similarity in fully differentiated matched Conditionally Reprogrammed (CRC) and Feeder free dual SMAD inhibited expanded human nasal epithelial cells Open
Background Patient-derived airway cells differentiated at Air Liquid Interface (ALI) are valuable models for Cystic fibrosis (CF) precision therapy. Advances in culture techniques have improved expansion capacity of airway basal cells, whi…
View article: Human Primary Epithelial Cell Models: Promising Tools in the Era of Cystic Fibrosis Personalized Medicine
Human Primary Epithelial Cell Models: Promising Tools in the Era of Cystic Fibrosis Personalized Medicine Open
Cystic fibrosis (CF) is an inherited disorder where individual disease etiology and response to therapeutic intervention is impacted by CF transmembrane regulator (CFTR) mutations and other genetic modifiers. CFTR regulates multiple mechan…
View article: The effect of premature termination codon mutations on <i>CFTR</i> mRNA abundance in human nasal epithelium and intestinal organoids: a basis for read‐through therapies in cystic fibrosis
The effect of premature termination codon mutations on <i>CFTR</i> mRNA abundance in human nasal epithelium and intestinal organoids: a basis for read‐through therapies in cystic fibrosis Open
A major challenge in cystic fibrosis (CF) research is applying mutation-specific therapy to individual patients with diverse and rare CF transmembrane conductance regulator (CFTR) genotypes. Read-through agents are currently the most promi…
View article: R560S: A class II CFTR mutation that is not rescued by current modulators
R560S: A class II CFTR mutation that is not rescued by current modulators Open
View article: WS15.1 From the bench to bedside: expanding CFTR modulators to rare mutations by response assessment in patients-derived materials and cellular models
WS15.1 From the bench to bedside: expanding CFTR modulators to rare mutations by response assessment in patients-derived materials and cellular models Open
View article: Partial rescue of F508del‐cystic fibrosis transmembrane conductance regulator channel gating with modest improvement of protein processing, but not stability, by a dual‐acting small molecule
Partial rescue of F508del‐cystic fibrosis transmembrane conductance regulator channel gating with modest improvement of protein processing, but not stability, by a dual‐acting small molecule Open
Background and Purpose Rescue of F508del‐cystic fibrosis (CF) transmembrane conductance regulator (CFTR), the most common CF mutation, requires small molecules that overcome protein processing, stability and channel gating defects. Here, w…
View article: Using a systems approach to identify the mechanism of action of correctors
Using a systems approach to identify the mechanism of action of correctors Open
Tese de doutoramento, Biologia (Biologia de Sistemas), Universidade de Lisboa, Faculdade de Ciências, 2018
View article: 29 Validating the organoid model across European laboratories
29 Validating the organoid model across European laboratories Open
View article: The third dimension: new developments in cell culture models for colorectal research
The third dimension: new developments in cell culture models for colorectal research Open
View article: Protein Traffic Disorders: an Effective High-Throughput Fluorescence Microscopy Pipeline for Drug Discovery
Protein Traffic Disorders: an Effective High-Throughput Fluorescence Microscopy Pipeline for Drug Discovery Open