Nigel J. Taylor
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View article: PlantGENE: Advancing plant transformation through community engagement
PlantGENE: Advancing plant transformation through community engagement Open
SUMMARY Plant transformation is an important part of plant research and crop improvement. Transformation methods remain complex, labor intensive, and inefficient. PlantGENE is a community of scientists from academia, industry, non‐profit r…
View article: Topology-inclusive aerodynamic shape optimisation using a cellular automata parameterisation
Topology-inclusive aerodynamic shape optimisation using a cellular automata parameterisation Open
A novel geometry parameterisation method constructed from a volume-of-solid driven cellular automata is presented. The method is capable of describing complex geometry of arbitrary topology using a set of volume-of-solid parameters applied…
View article: Trait Development and CRISPR-Enabled Improvement in the African Orphan Crop Fonio (Digitaria exilis)
Trait Development and CRISPR-Enabled Improvement in the African Orphan Crop Fonio (Digitaria exilis) Open
Fonio (Digitaria exilis), known as the "grain of life," is a vital food security crop grown by smallholder farmers across West Africa. Renowned for its short maturity period of 6-8 weeks, fonio provides essential harvests during periods of…
View article: Spatial models of cetacean density in European Atlantic waters based on SCANS-IV summer 2022 survey data
Spatial models of cetacean density in European Atlantic waters based on SCANS-IV summer 2022 survey data Open
The 4th iteration of large-scale line-transect surveys of cetaceans in European Atlantic waters (SCANS) was conducted in the summer of 2022. SCANS-IV covered a 1.7 million km2 study area stretching from the Strait of Gibraltar to southern …
View article: CRISPR-Cas9-mediated knockout of CYP79D1 and CYP79D2 in cassava attenuates toxic cyanogen production
CRISPR-Cas9-mediated knockout of CYP79D1 and CYP79D2 in cassava attenuates toxic cyanogen production Open
Cassava ( Manihot esculenta ) is a starchy root crop that supports over a billion people in tropical and subtropical regions of the world. This staple, however, produces the neurotoxin cyanide and requires processing for safe consumption. …
View article: Grain shattering by cell death and fracture in <i>Eragrostis tef</i>
Grain shattering by cell death and fracture in <i>Eragrostis tef</i> Open
Abscission, known as shattering in crop species, is a highly regulated process by which plants shed parts. Although shattering has been studied extensively in cereals and a number of regulatory genes have been identified, much diversity in…
View article: <i>Agrobacterium</i>‐mediated Genetic Transformation of Cassava
<i>Agrobacterium</i>‐mediated Genetic Transformation of Cassava Open
The storage root crop cassava ( Manihot esculenta Crantz) is predicted to remain central to future food and economic security for smallholder farming households and agricultural output in the tropics. Genetic improvement of cassava is requ…
View article: Mutations in DNA polymerase δ subunit 1 co-segregate with CMD2-type resistance to Cassava Mosaic Geminiviruses
Mutations in DNA polymerase δ subunit 1 co-segregate with CMD2-type resistance to Cassava Mosaic Geminiviruses Open
Cassava mosaic disease (CMD) suppresses cassava yields across the tropics. The dominant CMD2 locus confers resistance to cassava mosaic geminiviruses. It has been reported that CMD2-type landraces lose resistance after regeneration through…
View article: CRISPR/Cas9‐mediated tetra‐allelic mutation of the ‘Green Revolution’ <i>SEMIDWARF‐1</i> (<i>SD‐1</i>) gene confers lodging resistance in tef (<i>Eragrostis tef</i>)
CRISPR/Cas9‐mediated tetra‐allelic mutation of the ‘Green Revolution’ <i>SEMIDWARF‐1</i> (<i>SD‐1</i>) gene confers lodging resistance in tef (<i>Eragrostis tef</i>) Open
Summary Tef is a staple food and a valuable cash crop for millions of people in Ethiopia. Lodging is a major limitation to tef production, and for decades, the development of lodging resistant varieties proved difficult with conventional b…
View article: Mutations in DNA polymerase δ subunit 1 mediate CMD2-type resistance to Cassava Mosaic Geminiviruses
Mutations in DNA polymerase δ subunit 1 mediate CMD2-type resistance to Cassava Mosaic Geminiviruses Open
Cassava mosaic disease suppresses cassava yields across the tropics. The dominant CMD2 locus confers resistance to the cassava mosaic geminiviruses. It has been reported that CMD2-type landraces lose resistance after regeneration through d…
View article: Studies on Pure Mlb® (Multiple Left Border) Technology and Its Impact on Vector Backbone Integration in Transgenic Cassava
Studies on Pure Mlb® (Multiple Left Border) Technology and Its Impact on Vector Backbone Integration in Transgenic Cassava Open
Imperfect T-DNA processing is common during Agrobacterium -mediated transformation, which integrates vector backbone sequences into the plant genome. However, regulatory restrictions prevent such transgenic plants from being developed for …
View article: CRISPR-Cas9-mediated knockout of <i>CYP79D1</i> and <i>CYP79D2</i> in cassava attenuates toxic cyanogen production
CRISPR-Cas9-mediated knockout of <i>CYP79D1</i> and <i>CYP79D2</i> in cassava attenuates toxic cyanogen production Open
Summary Cassava ( Manihot esculenta Crantz) is a starchy root crop that supports over a billion people in tropical and subtropical regions of the world. This staple, however, produces toxic cyanogenic compounds and requires processing for …
View article: Agrobacterium-Mediated Cassava Transformation For The Asian-elite Variety KU50
Agrobacterium-Mediated Cassava Transformation For The Asian-elite Variety KU50 Open
Agrobacterium-mediated cassava transformation via friable embryogenic calli (FEC) has allowed the robust production of transgenic cassava. So far, transformation has been performed mostly for the model cassava variety 60444 and African var…
View article: Gene tagging via CRISPR-mediated homology-directed repair in cassava
Gene tagging via CRISPR-mediated homology-directed repair in cassava Open
Research on a few model plant–pathogen systems has benefitted from years of tool and resource development. This is not the case for the vast majority of economically and nutritionally important plants, creating a crop improvement bottlenec…
View article: Opportunities and challenges for biofortification of cassava to address iron and zinc deficiency in Nigeria
Opportunities and challenges for biofortification of cassava to address iron and zinc deficiency in Nigeria Open
Nigeria is the world's largest producer of cassava (Manihot esculenta), and its production is important to the country's economy. Cassava's edible storage roots act as a critical staple food for over 180 million Nigerians. Micronutrient de…
View article: Stacking disease resistance and mineral biofortification in cassava varieties to enhance yields and consumer health
Stacking disease resistance and mineral biofortification in cassava varieties to enhance yields and consumer health Open
Summary Delivering the benefits of agricultural biotechnology to smallholder farmers requires that resources be directed towards staple food crops. To achieve effect at scale, beneficial traits must be integrated into multiple, elite farme…
View article: Heterologous Overexpression of Arabidopsis cel1 Enhances Grain Yield, Biomass and Early Maturity in Setaria viridis
Heterologous Overexpression of Arabidopsis cel1 Enhances Grain Yield, Biomass and Early Maturity in Setaria viridis Open
Heterologous overexpression of Arabidopsis cellulase 1 (Atcel1) results in enhanced yield, early maturity, and increased biomass in dicotyledonous species like poplar and eucalyptus but has not been demonstrated in monocots. We produced tr…
View article: Comparative compositional analysis of cassava brown streak disease resistant 4046 cassava and its non-transgenic parental cultivar
Comparative compositional analysis of cassava brown streak disease resistant 4046 cassava and its non-transgenic parental cultivar Open
Compositional analysis is an important component of an integrated comparative approach to assessing the food and feed safety of new crops developed using biotechnology. As part of the safety assessment of cassava brown streak disease resis…
View article: Open access to genetic sequence data maximizes value to scientists, farmers, and society
Open access to genetic sequence data maximizes value to scientists, farmers, and society Open
Open access to genetic sequence data, often referred to as Digital Sequence Information, has been available since genome sequencing became possible and creates both monetary and nonmonetary value. Nonmonetary value is created when scientis…
View article: Visualizing cassava bacterial blight at the molecular level using CRISPR-mediated homology-directed repair
Visualizing cassava bacterial blight at the molecular level using CRISPR-mediated homology-directed repair Open
SUMMARY Research on a few model, plant-pathogen systems has benefitted from years of tool and resource development. This is not the case for the vast majority of economically and nutritionally important plants, creating a crop improvement …
View article: Cassava shrunken-2 homolog MeAPL3 determines storage root starch and dry matter content and modulates storage root postharvest physiological deterioration
Cassava shrunken-2 homolog MeAPL3 determines storage root starch and dry matter content and modulates storage root postharvest physiological deterioration Open
Key message Among the five cassava isoforms ( MeAPL1 – MeAPL5 ), MeAPL3 is responsible for determining storage root starch content. Degree of storage root postharvest physiological deterioration (PPD) is directly correlated with starch con…
View article: Engineering Disease-Resistant Cassava
Engineering Disease-Resistant Cassava Open
Manihot esculenta Crantz (cassava) is a food crop originating from South America grown primarily for its starchy storage roots. Today, cassava is grown in the tropics of South America, Africa, and Asia with an estimated 800 million people …
View article: Receptivity to Freestream Acoustic Noise in Hypersonic Flow over a Generic Forebody
Receptivity to Freestream Acoustic Noise in Hypersonic Flow over a Generic Forebody Open
Direct numerical simulations of the Navier–Stokes equations have been performed to investigate the receptivity and breakdown mechanisms in a Mach 6 flow over a generic forebody geometry with freestream acoustic disturbances. The simulation…