Niklas Capdeville
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Recent progress in plant genome engineering: from large insertions to chromosome number changes Open
The adaptation of the CRISPR/Cas system as a biotechnological tool has enabled a wide spectrum of targeted genome modifications. Whereas earlier approaches focused on small sequence changes, recent years have seen a shift toward larger-sca…
Increasing deletion sizes and the efficiency of <span>CRISPR</span>/Cas9‐mediated mutagenesis by <span>SunTag</span>‐mediated <span>TREX1</span> recruitment Open
SUMMARY Previously, it has been shown that mutagenesis frequencies can be improved by directly fusing the human exonuclease TREX2 to Cas9, resulting in a strong increase in the frequency of smaller deletions at the cut site. Here, we demon…
Optimizing <i>Er</i>Cas12a for efficient gene editing in <i>Arabidopsis thaliana</i> Open
Summary The Er Cas12a nuclease, also known as MAD7, is part of a CRISPR/Cas system from Eubacterium rectale and distantly related to Cas12a nucleases. As it shares only 31% sequence homology with the commonly used As Cas12a, its intellectu…
The DNA‐dependent protease AtWSS1A suppresses persistent double strand break formation during replication Open
Summary The protease WSS1A is an important factor in the repair of DNA‐protein crosslinks in plants. Here we show that the loss of WSS1A leads to a reduction of 45S rDNA repeats and chromosomal fragmentation in Arabidopsis. Moreover, in th…