Nikolaos Mougios
YOU?
Author Swipe
View article: A versatile microfluidics platform for enhanced multi-target super-resolution microscopy
A versatile microfluidics platform for enhanced multi-target super-resolution microscopy Open
DNA-based Point Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) is a powerful variant of single-molecule localization microscopy (SMLM) that overcomes the limitations of photobleaching, offers flexible fluorophore selection, a…
View article: Microtubules as a versatile reference standard for expansion microscopy
Microtubules as a versatile reference standard for expansion microscopy Open
Expansion microscopy (ExM) is continually improving, and new ExM variants need to be validated on well-defined biological structures. There is no consensus on validation structures for ExM, especially as nuclear pore complexes or DNA nanor…
View article: NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals
NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals Open
Fluorescence microscopy has long been a transformative technique in biological sciences. Nevertheless, most implementations are limited to a few targets, which have been revealed using primary antibodies and fluorescently conjugated second…
View article: One-step nanoscale expansion microscopy reveals individual protein shapes
One-step nanoscale expansion microscopy reveals individual protein shapes Open
The attainable resolution of fluorescence microscopy has reached the subnanometer range, but this technique still fails to image the morphology of single proteins or small molecular complexes. Here, we expand the specimens at least tenfold…
View article: Three-dimensional multi-target super-resolution microscopy of cells using Metal-Induced Energy Transfer and DNA-PAINT
Three-dimensional multi-target super-resolution microscopy of cells using Metal-Induced Energy Transfer and DNA-PAINT Open
Absrtact Achieving nanometer precision in 3D remains a major challenge in super-resolution microscopy. DNA-PAINT offers excellent lateral resolution and versatile multiplexing, but its axial localization precision is typically 3–5 times po…
View article: NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals
NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals Open
Fluorescence microscopy has long been a transformative technique in biological sciences. Nevertheless, most implementations are limited to a few targets, revealed using primary antibodies (1.Abs) and fluorescently conjugated secondary anti…
View article: Actinin‐4 controls survival signaling in B cells by limiting the lateral mobility of B‐cell antigen receptors
Actinin‐4 controls survival signaling in B cells by limiting the lateral mobility of B‐cell antigen receptors Open
The structure and dynamics of F‐actin networks in the cortical area of B cells control the signal efficiency of B‐cell antigen receptors (BCRs). Although antigen‐induced signaling has been studied extensively, the role of cortical F‐actin …
View article: Advancements in Nanobody Applications: From Live Synaptic Imaging to Multiplexed Fluorescence Microscopy and Nanoscale Topography Mapping
Advancements in Nanobody Applications: From Live Synaptic Imaging to Multiplexed Fluorescence Microscopy and Nanoscale Topography Mapping Open
Fluorescence microscopy facilitates biological research by enabling straightforward visualization of fluorescently labeled proteins in live and fixed samples. Despite significant progress in microscopy techniques, including those enabling …
View article: Novel nanobody-based tools for studying the synaptic vesicle life cycle
Novel nanobody-based tools for studying the synaptic vesicle life cycle Open
View article: Neuronal activity modulates the incorporation of newly translated PSD-95 into a robust structure as revealed by STED and MINFLUX
Neuronal activity modulates the incorporation of newly translated PSD-95 into a robust structure as revealed by STED and MINFLUX Open
The postsynaptic density component PSD-95 undergoes activity-dependent plasticity mechanisms that rely on protein synthesis and structural remodeling. How synaptic activity can influence these dynamics at the single synapse level remains u…
View article: A Versatile Synaptotagmin‐1 Nanobody Provides Perturbation‐Free Live Synaptic Imaging And Low Linkage‐Error in Super‐Resolution Microscopy
A Versatile Synaptotagmin‐1 Nanobody Provides Perturbation‐Free Live Synaptic Imaging And Low Linkage‐Error in Super‐Resolution Microscopy Open
Imaging of living synapses has relied for over two decades on the overexpression of synaptic proteins fused to fluorescent reporters. This strategy alters the stoichiometry of synaptic components and ultimately affects synapse physiology. …
View article: Heat denaturation enables multicolor X10-STED microscopy
Heat denaturation enables multicolor X10-STED microscopy Open
View article: A versatile synaptotagmin-1 nanobody provides perturbation-free live synaptic imaging and low linkage-error in super-resolution microscopy
A versatile synaptotagmin-1 nanobody provides perturbation-free live synaptic imaging and low linkage-error in super-resolution microscopy Open
Imaging of living synapses has relied for over two decades on the overexpression of synaptic proteins fused to fluorescent reporters. This strategy changes the stoichiometry of synaptic components and ultimately affects synapse physiology.…
View article: Trafficking proteins show limited differences in mobility across different postsynaptic spines
Trafficking proteins show limited differences in mobility across different postsynaptic spines Open
The function of the postsynaptic compartment is based on the presence and activity of postsynaptic receptors, whose dynamics are controlled by numerous scaffolding, signaling and trafficking proteins. Although the receptors and the scaffol…
View article: Heat denaturation enables multicolor X10-STED microscopy at single-digit nanometer resolution
Heat denaturation enables multicolor X10-STED microscopy at single-digit nanometer resolution Open
Expansion microscopy (ExM) improves imaging quality by physically enlarging the biological specimens. In principle, combining a large expansion factor with optical super-resolution should provide extremely high imaging precision. However, …
View article: Visualizing proteins by expansion microscopy
Visualizing proteins by expansion microscopy Open
Fluorescence imaging is one of the most versatile and widely-used tools in biology 1 . Although techniques to overcome the diffraction barrier were introduced more than two decades ago, and the nominal attainable resolution kept improving …
View article: Heat denaturation enables multicolor X10-STED microscopy at single-digit nanometer resolution
Heat denaturation enables multicolor X10-STED microscopy at single-digit nanometer resolution Open
Expansion microscopy (ExM) improves imaging quality by physically enlarging the biological specimens. In principle, combining a large expansion factor with optical super-resolution should provide extremely high imaging precision. However, …
View article: Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells
Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells Open
DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) is a powerful super-resolution technique highly suitable for multi-target (multiplexing) bio-imaging. However, multiplexed imaging of cells is still challenging due to …
View article: Taxation and competitiveness of the Greek travel agencies
Taxation and competitiveness of the Greek travel agencies Open
The purpose of this study is to investigate the impact of the tax burden on the travel agencies in Greece as compared to the corresponding effect of taxes on the travel agencies of the competitive countries, Spain, Italy, Croatia, Malta, C…