Nikos S. Hatzakis
YOU?
Author Swipe
View article: Fuel-Free Rolosense: Viral Sensing Using Diffusional Particle Tracking
Fuel-Free Rolosense: Viral Sensing Using Diffusional Particle Tracking Open
High-sensitivity viral diagnostics typically use PCR to detect and amplify viral nucleic acids which requires fluorescence reporters, enzymatic amplification, specialized equipment and can be time-consuming. In this work, we describe fuel-…
View article: Nanoparticles across mucosal barriers: Differentiating mucoadhesion from mucopenetration using single particle tracking
Nanoparticles across mucosal barriers: Differentiating mucoadhesion from mucopenetration using single particle tracking Open
Mucus is a viscoelastic, selectively permeable barrier that protects epithelial surfaces throughout the body. Its complex structure presents a major challenge for nanoparticle-based drug delivery, requiring a balance between mucoadhesion, …
View article: Polymer functionalized liposomes as universal nanocarriers for drug delivery: Single particle insights on size-dependent performance and intracellular behavior
Polymer functionalized liposomes as universal nanocarriers for drug delivery: Single particle insights on size-dependent performance and intracellular behavior Open
Nanomedicine requires smart delivery systems that are precise, robust, and universal. While liposomes are established vehicles in drug delivery, their full potential is challenged by limited stability, leakage, insufficient response and li…
View article: Methods for drug delivery and compatibility studies in an ex vivo mucus-covered Caco-2 cell monolayer model
Methods for drug delivery and compatibility studies in an ex vivo mucus-covered Caco-2 cell monolayer model Open
Oral drug delivery studies are increasingly done using the Caco-2 cell monolayer model overlaid with simple or complex in vitro mucus matrices as the model then becomes more robust towards otherwise cytotoxic biorelevant conditions. Howeve…
View article: Modulation of insulin aggregation by betaine and proline directly observed via real‐time super‐resolution microscopy
Modulation of insulin aggregation by betaine and proline directly observed via real‐time super‐resolution microscopy Open
Protein aggregation is associated with a spectrum of neurodegenerative diseases. Although many small ligands have been found to modulate or inhibit protein aggregation, their molecular mechanisms remain unclear. One reason for this is the …
View article: Biomimic opto-nanobiointerface enables multiscale biomodulation
Biomimic opto-nanobiointerface enables multiscale biomodulation Open
Virtually all organic material on Earth has been produced converting solar energy through photosynthesis in chloroplasts, a sack-like, double membrane organelle in plants and algae, where transmembrane electron transfer occurs from lumen t…
View article: Neuronal constitutive endolysosomal perforations enable α-synuclein aggregation by internalized PFFs
Neuronal constitutive endolysosomal perforations enable α-synuclein aggregation by internalized PFFs Open
Endocytosis, required for the uptake of receptors and their ligands, can also introduce pathological aggregates such as α-synuclein (α-syn) in Parkinson’s Disease. We show here the unexpected presence of intrinsically perforated endolysoso…
View article: Direct observation of assembly and function of trigger responsive lipase biohybrids
Direct observation of assembly and function of trigger responsive lipase biohybrids Open
Protein-polymer biohybrids mark a cutting edge in the creation of advanced materials designed for high performance under diverse conditions by combining the intrinsic stability and adaptability of synthetic polymers with the remarkable fun…
View article: Diverse Intracellular Trafficking of Insulin Analogs by Machine Learning-based Colocalization and Diffusion Analysis
Diverse Intracellular Trafficking of Insulin Analogs by Machine Learning-based Colocalization and Diffusion Analysis Open
Insulin signaling is crucial for maintaining cellular function and systemic homeostasis, with its dysregulation leading to metabolic disorders in particular diabetes. While insulin analogs are essential in type 1 diabetes treatment, their …
View article: Organelle bridges and nanodomain partitioning govern targeting of membrane-embedded proteins to lipid droplets
Organelle bridges and nanodomain partitioning govern targeting of membrane-embedded proteins to lipid droplets Open
Numerous metabolic enzymes translocate from the ER membrane bilayer to the lipid droplet (LD) monolayer, where they perform essential functions. Mislocalization of certain LD-targeted membrane proteins, including HSD17B13 and PNPLA3, is im…
View article: Defect‐Engineered Metal–Organic Frameworks as Nanocarriers for Pharmacotherapy: Insights into Intracellular Dynamics at The Single Particle Level
Defect‐Engineered Metal–Organic Frameworks as Nanocarriers for Pharmacotherapy: Insights into Intracellular Dynamics at The Single Particle Level Open
Nanoscale Metal–Organic Frameworks (nanoMOFs) are widely implemented in a host of assays involving drug delivery, biosensing catalysis, and bioimaging. However, the cell pathways and cell fate remain poorly understood. Here, a new fluoresc…
View article: Proteolytic Performance is Dependent on Binding Efficiency, Processivity and Turnover: Single Protease Insights
Proteolytic Performance is Dependent on Binding Efficiency, Processivity and Turnover: Single Protease Insights Open
Proteases are essential enzymes for a plethora of biological processes and biotechnological applications, e.g., within the dairy, pharmaceutical, and detergent industries. Decoding the molecular level mechanisms that drive protease perform…
View article: A systematic analysis of regression models for protein engineering
A systematic analysis of regression models for protein engineering Open
To optimize proteins for particular traits holds great promise for industrial and pharmaceutical purposes. Machine Learning is increasingly applied in this field to predict properties of proteins, thereby guiding the experimental optimizat…
View article: Defect-Engineered Metal-Organic Frameworks as Nanocarriers for Pharmacotherapy: Insights into Intracellular Dynamics at The Single Particle Level
Defect-Engineered Metal-Organic Frameworks as Nanocarriers for Pharmacotherapy: Insights into Intracellular Dynamics at The Single Particle Level Open
NanoMOFs are widely implemented in a host of assays involving drug delivery, biosensing catalysis, and bioimaging. Despite their wide use, the cell entry pathways and cell fate remain poorly understood. Here we have synthesized a new fluor…
View article: Oxygen-tolerant, Eosin Y mediated synthesis of protein-polymer biohybrids and protein-coated polymer nanoparticles
Oxygen-tolerant, Eosin Y mediated synthesis of protein-polymer biohybrids and protein-coated polymer nanoparticles Open
To avoid the use of metal catalysts used in conventional approaches, an Eosin Y/TEMED mediated, photoinduced polymerization of vinyl monomers was optimized for the synthesis of protein-polymer conjugates. This oxygen tolerant, photoinduced…
View article: Deconvoluting the Effect of Cell-Penetrating Peptides for Enhanced and Controlled Insertion of Large-Scale DNA Nanopores
Deconvoluting the Effect of Cell-Penetrating Peptides for Enhanced and Controlled Insertion of Large-Scale DNA Nanopores Open
DNA nanopores have emerged as powerful tools for molecular sensing, but the efficient insertion of large DNA nanopores into lipid membranes remains challenging. In this study, we investigate the potential of cell-penetrating peptides (CPPs…
View article: Neonatal intestinal mucus barrier changes in response to maturity, inflammation, and sodium decanoate supplementation
Neonatal intestinal mucus barrier changes in response to maturity, inflammation, and sodium decanoate supplementation Open
The integrity of the intestinal mucus barrier is crucial for human health, as it serves as the body's first line of defense against pathogens. However, postnatal development of the mucus barrier and interactions between maturity and its ab…
View article: Probing Activation and Conformational Dynamics of the Vesicle-Reconstituted β<sub>2</sub> Adrenergic Receptor at the Single-Molecule Level
Probing Activation and Conformational Dynamics of the Vesicle-Reconstituted β<sub>2</sub> Adrenergic Receptor at the Single-Molecule Level Open
G-protein-coupled receptors (GPCRs) are structurally flexible membrane proteins that mediate a host of physiological responses to extracellular ligands like hormones and neurotransmitters. Fine features of their dynamic structural behavior…
View article: Deep learning assisted single particle tracking for automated correlation between diffusion and function
Deep learning assisted single particle tracking for automated correlation between diffusion and function Open
Sub-cellular diffusion in living systems reflects cellular processes and interactions. Recent advances in optical microscopy allow the tracking of this nanoscale diffusion of individual objects with an unprecedented level of precision. How…
View article: Everything, everywhere, almost at once
Everything, everywhere, almost at once Open
A new platform that can follow the movement of individual proteins inside millions of cells in a single day will help contribute to existing knowledge of cell biology and identify new therapeutics.
View article: Constitutive Endolysosomal Perforation in Neurons allows Induction of α-Synuclein Aggregation by Internalized Pre-Formed Fibrils
Constitutive Endolysosomal Perforation in Neurons allows Induction of α-Synuclein Aggregation by Internalized Pre-Formed Fibrils Open
The endocytic pathway is both an essential route of molecular uptake in cells and a potential entry point for pathology-inducing cargo. The cell-to-cell spread of cytotoxic aggregates, such as those of α-synuclein (α-syn) in Parkinson’s Di…
View article: Oxygen-tolerant, eosin Y mediated synthesis of protein–polymer biohybrids and protein-coated polymer nanoparticles
Oxygen-tolerant, eosin Y mediated synthesis of protein–polymer biohybrids and protein-coated polymer nanoparticles Open
An oxygen tolerant, eosin Y/TEMED mediated, photoinduced polymerization approach was optimized to graft hydrophobic, hydrophilic, and responsive polymers from protein macroinitiators, avoiding conventional metal catalysts.