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View article: Engineering Protein Scaffold-Assisted C10-C35 Terpene Precursors Overproduction System for Efficient Biosynthesis Studies
Engineering Protein Scaffold-Assisted C10-C35 Terpene Precursors Overproduction System for Efficient Biosynthesis Studies Open
Terpenoids are widely used in the food, cosmetics, and pharmaceutical fields. However, the application is hindered by insufficient supplies of natural origin. The microbial biosynthesis presents an alternative to conventional techniques. H…
View article: The thiolation of uridine 34 in tRNA, which controls protein translation, depends on a [4Fe-4S] cluster in the archaeum Methanococcus maripaludis
The thiolation of uridine 34 in tRNA, which controls protein translation, depends on a [4Fe-4S] cluster in the archaeum Methanococcus maripaludis Open
Thiolation of uridine 34 in the anticodon loop of several tRNAs is conserved in the three domains of life and guarantees fidelity of protein translation. U34-tRNA thiolation is catalyzed by a complex of two proteins in the eukaryotic cytos…
View article: A subclass of archaeal U8-tRNA sulfurases requires a [4Fe–4S] cluster for catalysis
A subclass of archaeal U8-tRNA sulfurases requires a [4Fe–4S] cluster for catalysis Open
Sulfuration of uridine 8, in bacterial and archaeal tRNAs, is catalyzed by enzymes formerly known as ThiI, but renamed here TtuI. Two different classes of TtuI proteins, which possess a PP-loop-containing pyrophosphatase domain that includ…
View article: Determination of the Absolute Molar Mass of [Fe-S]-Containing Proteins Using Size Exclusion Chromatography-Multi-Angle Light Scattering (SEC-MALS)
Determination of the Absolute Molar Mass of [Fe-S]-Containing Proteins Using Size Exclusion Chromatography-Multi-Angle Light Scattering (SEC-MALS) Open
Size Exclusion Chromatography coupled with Multi-Angle Light Scattering (SEC-MALS) is a technique that determines the absolute molar mass (molecular weight) of macromolecules in solution, such as proteins or polymers, by detecting their li…
View article: High-Level Extracellular Expression of a New β-N-Acetylglucosaminidase in Escherichia coli for Producing GlcNAc
High-Level Extracellular Expression of a New β-N-Acetylglucosaminidase in Escherichia coli for Producing GlcNAc Open
N-acetyl-β-D glucosamine (GlcNAc) is wildly used in cosmetics, nutraceuticals and pharmaceuticals. The traditional chemical process for GlcNAc production from chitin causes serious acidic pollution. Therefore, the enzymatic hydrolysis beco…
View article: Expression and Regulation of pde6h by Thyroid Hormone During Metamorphosis in Paralichthys olivaceus
Expression and Regulation of pde6h by Thyroid Hormone During Metamorphosis in Paralichthys olivaceus Open
PDE6H is a cone cell-specific inhibitory subunit that plays a critical role in the adaptation of the photosensitive system to bright and dark phases of the light environment. Thyroid hormone (TH) is one of the most important factors that c…
View article: Construction of an octosyl acid backbone catalyzed by a radical S-adenosylmethionine enzyme and a phosphatase in the biosynthesis of high-carbon sugar nucleoside antibiotics
Construction of an octosyl acid backbone catalyzed by a radical S-adenosylmethionine enzyme and a phosphatase in the biosynthesis of high-carbon sugar nucleoside antibiotics Open
This work provides, for the first time, significantin vitroevidence for the biosynthetic origins of octosyl acid through free radical and dephosphorylation enzymatic reactions.
View article: Structural basis of rifampin inactivation by rifampin phosphotransferase
Structural basis of rifampin inactivation by rifampin phosphotransferase Open
Significance Rifampin phosphotransferases (RPH) belong to a recently identified antibiotic-resistance protein family that inactivates rifampin, the first-line drug against tuberculosis, by phosphorylation. By determining the structures of …