Nishith Gupta
YOU?
Author Swipe
View article: New mechanistic insights into macrophage extracellular trap formation induced by a parasitic nematode, Strongyloides stercoralis
New mechanistic insights into macrophage extracellular trap formation induced by a parasitic nematode, Strongyloides stercoralis Open
Macrophages execute host defense against pathogens by releasing extracellular traps (METs) composed of DNA meshwork and antimicrobial proteins. Although MET-mediated pathogen immobilization is well documented, the induction mechanisms of M…
View article: Site- and Structure-Specific Characterization of Glycoproteins of H11: Potent Vaccine Candidates against Parasitic Worm <i>Haemonchus</i>
Site- and Structure-Specific Characterization of Glycoproteins of H11: Potent Vaccine Candidates against Parasitic Worm <i>Haemonchus</i> Open
Parasitic worms (helminths) infections pose significant threats to global health and livestock economies. While mass spectrometry (MS)-based glycomics have revealed that helminths express complex, immunomodulatory N-glycans, site- and stru…
View article: Sarcoendoplasmic reticulum calcium ATPase is an essential and druggable lipid-dependent ion pump in Toxoplasma gondii
Sarcoendoplasmic reticulum calcium ATPase is an essential and druggable lipid-dependent ion pump in Toxoplasma gondii Open
Toxoplasma gondii is a common intracellular pathogenic protist causing acute and chronic infections in many warm-blooded organisms. Calcium homeostasis is pivotal for its asexual reproduction in mammalian host cells, and sarcoendoplasmic r…
View article: Metabolic crosstalk between the mitochondrion and the nucleus is essential for Toxoplasma gondii infection
Metabolic crosstalk between the mitochondrion and the nucleus is essential for Toxoplasma gondii infection Open
Toxoplasma gondii, an intracellular pathogenic protist with a remarkable ability to infect a wide range of host cells, displays an equally exceptional design of its carbon metabolism. There are, however, critical gaps in our understanding …
View article: In vitro culture of the parasitic stage larvae of hematophagous parasitic nematode Haemonchus contortus
In vitro culture of the parasitic stage larvae of hematophagous parasitic nematode Haemonchus contortus Open
Current research on common parasitic nematodes is limited because their infective stages cannot be propagated in vitro. Here, we report a culture system for developing L4s of Haemonchus contortus, a blood-feeding nematode of ruminants. Our…
View article: Expression of IL-1β in transgenic Eimeria necatrix enhances the immunogenicity of parasites and promotes mucosal immunity against coccidiosis
Expression of IL-1β in transgenic Eimeria necatrix enhances the immunogenicity of parasites and promotes mucosal immunity against coccidiosis Open
Anticoccidial vaccines comprising living oocysts of Eimeria tenella, Eimeria necatrix, Eimeria maxima , and Eimeria acervulina are used to control coccidiosis. This study explored the potential of IL-1β to act as a molecular adjuvant for e…
View article: A pyruvate transporter in the apicoplast of apicomplexan parasites
A pyruvate transporter in the apicoplast of apicomplexan parasites Open
Pyruvate lies at a pivotal node of carbon metabolism in eukaryotes. It is involved in diverse metabolic pathways in multiple organelles, and its interorganelle shuttling is crucial for cell fitness. Many apicomplexan parasites harbor a uni…
View article: Metabolic plasticity, essentiality and therapeutic potential of ribose-5-phosphate synthesis in Toxoplasma gondii
Metabolic plasticity, essentiality and therapeutic potential of ribose-5-phosphate synthesis in Toxoplasma gondii Open
Ribose-5-phosphate (R5P) is a precursor for nucleic acid biogenesis; however, the importance and homeostasis of R5P in the intracellular parasite Toxoplasma gondii remain enigmatic. Here, we show that the cytoplasmic sedoheptulose-1,7-bisp…
View article: AP2XII-1 is a negative regulator of merogony and presexual commitment in <i>Toxoplasma gondii</i>
AP2XII-1 is a negative regulator of merogony and presexual commitment in <i>Toxoplasma gondii</i> Open
A transition from the asexual to sexual development in the widespread intracellular pathogen Toxoplasma gondii requires stage switching in its feline (definitive) host, but the mechanisms governing this process remain elusive. Here, we dem…
View article: The Mitochondrial Pyruvate Carrier Coupling Glycolysis and the Tricarboxylic Acid Cycle Is Required for the Asexual Reproduction of Toxoplasma gondii
The Mitochondrial Pyruvate Carrier Coupling Glycolysis and the Tricarboxylic Acid Cycle Is Required for the Asexual Reproduction of Toxoplasma gondii Open
T. gondii is a zoonotic parasite capable of infecting many warm-blooded organisms, including humans. Among others, a feature that allows it to parasitize multiple hosts is its exceptional metabolic plasticity.
View article: Apically-located P4-ATPase1-Lem1 complex internalizes phosphatidylserine and regulates motility-dependent invasion and egress in Toxoplasma gondii
Apically-located P4-ATPase1-Lem1 complex internalizes phosphatidylserine and regulates motility-dependent invasion and egress in Toxoplasma gondii Open
The membrane asymmetry regulated by P4-ATPases is crucial for the functioning of eukaryotic cells. The underlying spatial translocation or flipping of specific lipids is usually assured by respective P4-ATPases coupled to conforming non-ca…
View article: Toxoplasma gondii and Neospora caninum Antibodies in Dogs and Cats from Egypt and Risk Factor Analysis
Toxoplasma gondii and Neospora caninum Antibodies in Dogs and Cats from Egypt and Risk Factor Analysis Open
Background: Toxoplasma gondii and Neospora caninum are major protozoan parasites of worldwide distribution and significance in veterinary medicine and, for T. gondii, in public health. Cats and dogs, as final hosts for T. gondii and N. can…
View article: H11-induced immunoprotection is predominantly linked to N-glycan moieties during Haemonchus contortus infection
H11-induced immunoprotection is predominantly linked to N-glycan moieties during Haemonchus contortus infection Open
Nematodes are one of the largest groups of animals on the planet. Many of them are major pathogens of humans, animals and plants, and cause destructive diseases and socioeconomic losses worldwide. Despite their adverse impacts on human hea…
View article: Synthesis versus Salvage of Ester- and Ether-linked Phosphatidylethanolamine in the Intracellular Protozoan Pathogen Toxoplasma gondii
Synthesis versus Salvage of Ester- and Ether-linked Phosphatidylethanolamine in the Intracellular Protozoan Pathogen Toxoplasma gondii Open
Toxoplasma gondii is a prevalent zoonotic pathogen infecting livestock as well as humans. The exceptional ability of this parasite to reproduce in several types of nucleated host cells necessitates a coordinated usage of endogenous and hos…
View article: Metabolic flexibilities and vulnerabilities in the pentose phosphate pathway of the zoonotic pathogen Toxoplasma gondii
Metabolic flexibilities and vulnerabilities in the pentose phosphate pathway of the zoonotic pathogen Toxoplasma gondii Open
Metabolic pathways underpin the growth and virulence of intracellular parasites and are therefore promising antiparasitic targets. The pentose phosphate pathway (PPP) is vital in most organisms, providing a reduced form of nicotinamide ade…
View article: Performance Evaluation of Irrigation Automation for Cultivation System of Rice Intensification (SRI) Combined with Jajarlegowo System: Theories, Methods, and Techniques
Performance Evaluation of Irrigation Automation for Cultivation System of Rice Intensification (SRI) Combined with Jajarlegowo System: Theories, Methods, and Techniques Open
The objective of this article is to review and represent about automation irrigations that have been gained in irrigation system performance evaluation for cultivation System of Rice Intensification (SRI) combined with Jajarlegowo System. …
View article: A novel BR-SMAD is required for larval development in barber’s pole worm Haemonchus contortus
A novel BR-SMAD is required for larval development in barber’s pole worm Haemonchus contortus Open
SMAD proteins mediate TGF-β signaling and thereby regulate the metazoan development; however, they are poorly defined in Haemonchus contortus–a common blood-sucking parasitic nematode of small ruminants. Here, we characterized an R-SMAD fa…
View article: Cluster Frontline Demonstrations Impact on Productivity of Soybean in Dewas District of Malwa Plateau
Cluster Frontline Demonstrations Impact on Productivity of Soybean in Dewas District of Malwa Plateau Open
Cluster Front line demonstrations on soybean variety JS 9560 were organized at 200 farmers field consecutively for two years in kharif seasons of 2016 and 2017 in many adopted villages of dewas district by Krishi Vigyan Kendra, Dewas.The p…
View article: Aminoglycerophospholipid flipping and P4-ATPases in Toxoplasma gondii
Aminoglycerophospholipid flipping and P4-ATPases in Toxoplasma gondii Open
Lipid flipping in the membrane bilayers is a widespread eukaryotic phenomenon that is catalyzed by assorted P4-ATPases. Its occurrence, mechanism, and importance in apicomplexan parasites have remained elusive, however. Here we show that T…
View article: Toxoplasma and Eimeria co-opt the host cFos expression for intracellular development in mammalian cells
Toxoplasma and Eimeria co-opt the host cFos expression for intracellular development in mammalian cells Open
Successful asexual reproduction of intracellular pathogens depends on their potential to exploit host resources and subvert antimicrobial defense. In this work, we deployed two prevalent apicomplexan parasites of mammalian cells, namely To…
View article: Evaluation of Kharif Onion (Allium cepa L.) Varieties in Malwa Agro Climatic Zone of Madhya Pradesh
Evaluation of Kharif Onion (Allium cepa L.) Varieties in Malwa Agro Climatic Zone of Madhya Pradesh Open
The on farm testing was conducted in the farmers’ fields of Dewas district of Madhya Pradesh with the objective to find out the most suitable varieties of kharif onion for two consecutive years i.e. 2018-19 and 2019-20. From the study it i…
View article: Haemophagocytic lymphohistiocytosis (HLH) in human immunodeficiency virus (HIV): A systematic review of literature
Haemophagocytic lymphohistiocytosis (HLH) in human immunodeficiency virus (HIV): A systematic review of literature Open
Background: Hemophagocytic lymphohistiocytosis (HLH) is a hyper-immune condition secondary to malignancy, infection or auto-immune conditions. HLH in patients with human immunodeficiency virus infection (HIV) can be either be due to a co-e…
View article: Acquisition of exogenous fatty acids renders apicoplast-based biosynthesis dispensable in tachyzoites of Toxoplasma
Acquisition of exogenous fatty acids renders apicoplast-based biosynthesis dispensable in tachyzoites of Toxoplasma Open
Toxoplasma gondii is a common protozoan parasite that infects a wide range of hosts, including livestock and humans. Previous studies have suggested that the type 2 fatty acid synthesis (FAS2) pathway, located in the apicoplast (a nonphoto…