Noa Lipstein
YOU?
Author Swipe
View article: Peroxisome protein import deficiency causes heart failure in mouse and human
Peroxisome protein import deficiency causes heart failure in mouse and human Open
Peroxisomes are ubiquitous cellular organelles with potentially vital roles in lipid and reactive oxygen metabolism. The metabolic demands of the heart are substantial; however, the contribution of peroxisomes to cardiac development, healt…
View article: Ca<sup>2+</sup>-phospholipid–dependent regulation of Munc13-1 is essential for post-tetanic potentiation at mossy fiber synapses and supports working memory
Ca<sup>2+</sup>-phospholipid–dependent regulation of Munc13-1 is essential for post-tetanic potentiation at mossy fiber synapses and supports working memory Open
SUMMARY Hippocampal mossy fiber (hMF) to CA3 pyramidal cell synapses are thought to support the formation of working memory through presynaptic short-term facilitation (STF) and post-tetanic potentiation (PTP). However, the molecular mecha…
View article: Proximity labelling reveals the compartmental proteome of murine sensory neurons
Proximity labelling reveals the compartmental proteome of murine sensory neurons Open
Introduction Understanding the molecular architecture of peripheral sensory neurons is critical as we pursue novel drug targets against pain and neuropathy. Sensory neurons in the dorsal root ganglion (DRG) show extensive compartmentalizat…
View article: Endogenous SNAP-Tagging of Munc13-1 for Monitoring Synapse Nanoarchitecture
Endogenous SNAP-Tagging of Munc13-1 for Monitoring Synapse Nanoarchitecture Open
Synaptic function is governed by highly regulated protein machineries, whose abundance and spatial localization change continually. Studies to determine dynamic changes in synaptic protein nanoarchitecture typically rely on immunolabeling …
View article: Number and relative abundance of synaptic vesicles in functionally distinct priming states determine synaptic strength and short‐term plasticity
Number and relative abundance of synaptic vesicles in functionally distinct priming states determine synaptic strength and short‐term plasticity Open
Heterogeneity in synaptic strength and short‐term plasticity (STP) was characterized in post‐hearing rat calyx of Held synapses at near‐physiological external [Ca 2+ ] under control conditions and after experimentally induced synaptic pote…
View article: Highly Specific Miniaturized Fluorescent Monoacylglycerol Lipase Probes Enable Translational Research
Highly Specific Miniaturized Fluorescent Monoacylglycerol Lipase Probes Enable Translational Research Open
Monoacylglycerol lipase (MAGL) is the pivotal catabolic enzyme responsible for signal termination in the endocannabinoid system. Inhibition of MAGL offers unique advantages over the direct activation of cannabinoid receptors in treating ca…
View article: A Highly Selective and Versatile Probe Platform for Visualization of Monoacylglycerol Lipase
A Highly Selective and Versatile Probe Platform for Visualization of Monoacylglycerol Lipase Open
Monoacylglycerol lipase (MAGL) is a key enzyme for signal termination in the endocannabinoid system (ECS). MAGL inhibition results in indirect activation of the cannabinoid receptors, which offers unique advantages for the treatment of, e.…
View article: A Highly Selective and Versatile Probe Platform for Visualization of Monoacylglycerol Lipase
A Highly Selective and Versatile Probe Platform for Visualization of Monoacylglycerol Lipase Open
Monoacylglycerol lipase (MAGL) is a key enzyme for signal termination in the endocannabinoid system (ECS). MAGL inhibition results in indirect activation of the cannabinoid receptors, which offers unique advantages for the treatment of, e.…
View article: Chronic stress induces depression through MDGA1-Neuroligin2 mediated suppression of inhibitory synapses in the lateral habenula
Chronic stress induces depression through MDGA1-Neuroligin2 mediated suppression of inhibitory synapses in the lateral habenula Open
Rationale: The hyperactivity of lateral habenula (LHb) has been implicated in the pathophysiology of depression, but the regulatory mechanisms of inhibitory synapses in this context remains unclear. MDGA1 and neuroligin2 (Nlgn2), both regu…
View article: Investigating the Molecular Composition of Neuronal Subcompartments Using Proximity Labeling
Investigating the Molecular Composition of Neuronal Subcompartments Using Proximity Labeling Open
The expression pattern of proteins defines the range of biological processes in cellular subcompartments. A core aim in cell biology is therefore to determine the localization and composition of protein complexes within cells. Proximity la…
View article: Endogenous SNAP-tagging of Munc13-1 for monitoring synapse nanoarchitecture
Endogenous SNAP-tagging of Munc13-1 for monitoring synapse nanoarchitecture Open
Synaptic function is governed by highly regulated protein machineries, whose abundance and spatial localization change continually. Studies to determine the dynamic changes in synaptic proteins nanoarchitecture typically rely on immunolabe…
View article: The synaptic vesicle cluster as a controller of pre‐ and postsynaptic structure and function
The synaptic vesicle cluster as a controller of pre‐ and postsynaptic structure and function Open
The synaptic vesicle cluster (SVC) is an essential component of chemical synapses, which provides neurotransmitter‐loaded vesicles during synaptic activity, at the same time as also controlling the local concentrations of numerous exo‐ and…
View article: A minor impact of VGLUT1 expression level on quantal size revealed through the characterization of VGLUT1<sup>mEos2</sup>knock-down new mouse model
A minor impact of VGLUT1 expression level on quantal size revealed through the characterization of VGLUT1<sup>mEos2</sup>knock-down new mouse model Open
Synaptic vesicles (SVs) are small organelles secreting neurotransmitters at synapses. By fusing a photoactivated fluorescent protein to VGLUT1, we generated a VGLUT1 mEos2 knock-in mouse. VGLUT1 mEos2 knock-in mice are viable and healthy, …
View article: A one-step protocol to generate impermeable fluorescent HaloTag substrates for<i>in situ</i>live cell application and super-resolution imaging
A one-step protocol to generate impermeable fluorescent HaloTag substrates for<i>in situ</i>live cell application and super-resolution imaging Open
Communication between cells is largely orchestrated by proteins on the cell surface, which allow information transfer across the cell membrane. Super-resolution and single-molecule visualization of these proteins can be achieved by genetic…
View article: Complexin has a dual synaptic function as checkpoint protein in vesicle priming and as a promoter of vesicle fusion
Complexin has a dual synaptic function as checkpoint protein in vesicle priming and as a promoter of vesicle fusion Open
The presynaptic SNARE-complex regulator complexin (Cplx) enhances the fusogenicity of primed synaptic vesicles (SVs). Consequently, Cplx deletion impairs action potential-evoked transmitter release. Conversely, though, Cplx loss enhances s…
View article: Ketamine potentiates a central glutamatergic presynapse
Ketamine potentiates a central glutamatergic presynapse Open
Ketamine produces rapid and sustained antidepressant effects after brief exposure to a single dose. Counterintuitively, while ketamine acts primarily as a blocker of postsynaptic N-methyl-D-aspartate receptors (NMDARs), increased signallin…
View article: Phorbolester-activated Munc13-1 and ubMunc13-2 exert opposing effects on dense-core vesicle secretion
Phorbolester-activated Munc13-1 and ubMunc13-2 exert opposing effects on dense-core vesicle secretion Open
Munc13 proteins are priming factors for SNARE-dependent exocytosis, which are activated by diacylglycerol (DAG)-binding to their C1-domain. Several Munc13 paralogs exist, but their differential roles are not well understood. We studied the…
View article: Author response: Phorbolester-activated Munc13-1 and ubMunc13-2 exert opposing effects on dense-core vesicle secretion
Author response: Phorbolester-activated Munc13-1 and ubMunc13-2 exert opposing effects on dense-core vesicle secretion Open
Article Figures and data Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Munc13 proteins are pr…
View article: Phorbolester-activated Munc13-1 and ubMunc13-2 exert opposing effects on dense-core vesicle secretion
Phorbolester-activated Munc13-1 and ubMunc13-2 exert opposing effects on dense-core vesicle secretion Open
Munc13 proteins are priming factors for SNARE-dependent exocytosis, which are activated by diacylglycerol (DAG)-binding to their C1-domain. Several Munc13 paralogs exist, but their differential roles are not well understood. We studied the…
View article: Molecular and functional architecture of striatal dopamine release sites
Molecular and functional architecture of striatal dopamine release sites Open
Dopamine controls striatal circuit function, but its transmission mechanisms are not well understood. We recently showed that dopamine secretion requires RIM, suggesting that it occurs at active zone-like sites similar to conventional syna…
View article: Delineating the Molecular Basis of the Calmodulin–bMunc13-2 Interaction by Cross-Linking/Mass Spectrometry—Evidence for a Novel CaM Binding Motif in bMunc13-2
Delineating the Molecular Basis of the Calmodulin–bMunc13-2 Interaction by Cross-Linking/Mass Spectrometry—Evidence for a Novel CaM Binding Motif in bMunc13-2 Open
Exploring the interactions between the Ca2+ binding protein calmodulin (CaM) and its target proteins remains a challenging task. Members of the Munc13 protein family play an essential role in short-term synaptic plasticity, modulated via t…
View article: Apparent calcium dependence of vesicle recruitment
Apparent calcium dependence of vesicle recruitment Open
Key points Synaptic transmission relies on the recruitment of neurotransmitter‐filled vesicles to presynaptic release sites. Increased intracellular calcium buffering slows the recovery from synaptic depression, suggesting that vesicle rec…
View article: Synaptic UNC13A protein variant causes increased neurotransmission and dyskinetic movement disorder
Synaptic UNC13A protein variant causes increased neurotransmission and dyskinetic movement disorder Open
Munc13 proteins are essential regulators of neurotransmitter release at nerve cell synapses. They mediate the priming step that renders synaptic vesicles fusion-competent, and their genetic elimination causes a complete block of synaptic t…