Alex J. Noble
YOU?
Author Swipe
View article: VirtualIce: Half-synthetic CryoEM Micrograph Generator
VirtualIce: Half-synthetic CryoEM Micrograph Generator Open
Single particle cryo-electron microscopy (cryoEM) is going through a phase of rapid optimization focused on increasing the efficiency, accuracy, and automation of every step in the data pipeline. Machine learning models in particular have …
View article: Myosin forces elicit an F-actin structural landscape that mediates mechanosensitive protein recognition
Myosin forces elicit an F-actin structural landscape that mediates mechanosensitive protein recognition Open
Cells mechanically interface with their surroundings through cytoskeleton-linked adhesions, allowing them to sense physical cues that instruct development and drive diseases such as cancer. Contractile forces generated by myosin motor prot…
View article: Cryo-focused ion beam for in situ structural biology: State of the art, challenges, and perspectives
Cryo-focused ion beam for in situ structural biology: State of the art, challenges, and perspectives Open
Cryogenic-focused ion beam (cryo-FIB) instruments became essential for high-resolution imaging in cryo-preserved cells and tissues. Cryo-FIBs use accelerated ions to thin samples that would otherwise be too thick for cryo-electron microsco…
View article: CryoCycle your grids: Plunge vitrifying and reusing clipped grids to advance cryoEM democratization
CryoCycle your grids: Plunge vitrifying and reusing clipped grids to advance cryoEM democratization Open
CryoEM democratization is hampered by access to costly plunge-freezing supplies. We introduce methods, called CryoCycle, for reliably blotting, vitrifying, and reusing clipped cryoEM grids. We demonstrate that vitreous ice may be produced …
View article: Cryo-Electron Microscopy Screening Automation across Multiple Grids using Smart Leginon
Cryo-Electron Microscopy Screening Automation across Multiple Grids using Smart Leginon Open
Advancements in cryo-electron microscopy (cryoEM) techniques over the past decade have allowed structural biologists to routinely resolve macromolecular protein complexes to near-atomic resolution. The general workflow of the entire cryoEM…
View article: Heterogeneous non-canonical nucleosomes predominate in yeast cells in situ
Heterogeneous non-canonical nucleosomes predominate in yeast cells in situ Open
Nuclear processes depend on the organization of chromatin, whose basic units are cylinder-shaped complexes called nucleosomes. A subset of mammalian nucleosomes in situ (inside cells) resembles the canonical structure determined in vitro 2…
View article: Smart Leginon enables cryoEM screening automation across multiple grids
Smart Leginon enables cryoEM screening automation across multiple grids Open
Summary CryoEM multi-grid screening is often a tedious process that demands hours of attention. Here, this protocol shows how to set up standard Leginon collection and Smart Leginon Autoscreen to automate this process. This protocol can be…
View article: Author Response: Heterogeneous non-canonical nucleosomes predominate in yeast cells in situ
Author Response: Heterogeneous non-canonical nucleosomes predominate in yeast cells in situ Open
Nuclear processes depend on the organization of chromatin, whose basic units are cylinder-shaped complexes called nucleosomes. A subset of mammalian nucleosomes in situ (inside cells) resembles the canonical structure determined in vitro 2…
View article: Heterogeneous non-canonical nucleosomes predominate in yeast cells in situ
Heterogeneous non-canonical nucleosomes predominate in yeast cells in situ Open
Summary Nuclear processes depend on the organization of chromatin, whose basic units are cylinder-shaped complexes called nucleosomes. A subset of mammalian nucleosomes in situ (inside cells) resembles the canonical structure determined in…
View article: Addressing educational gaps through multidisciplinary team education in eosinophilic oesophagitis management
Addressing educational gaps through multidisciplinary team education in eosinophilic oesophagitis management Open
Once considered a rare disease, eosinophilic oesophagitis (EoE) is becoming increasingly prevalent, yet many healthcare professionals (HCPs) remain unfamiliar with the underlying pathophysiology and optimal management approaches. For this …
View article: Heterogeneous non-canonical nucleosomes predominate in yeast cells in situ
Heterogeneous non-canonical nucleosomes predominate in yeast cells in situ Open
Nuclear processes depend on the organization of chromatin, whose basic units are cylinder-shaped complexes called nucleosomes. A subset of mammalian nucleosomes in situ (inside cells) resembles the canonical structure determined in vitro 2…
View article: Heterogeneous non-canonical nucleosomes predominate in yeast cells in situ
Heterogeneous non-canonical nucleosomes predominate in yeast cells in situ Open
Summary Nuclear processes depend on the organization of chromatin, whose basic units are cylinder-shaped complexes called nucleosomes. A subset of mammalian nucleosomes in situ (inside cells) resembles the canonical structure determined in…
View article: Author Response: Heterogeneous non-canonical nucleosomes predominate in yeast cells in situ
Author Response: Heterogeneous non-canonical nucleosomes predominate in yeast cells in situ Open
Nuclear processes depend on the organization of chromatin, whose basic units are cylinder-shaped complexes called nucleosomes. A subset of mammalian nucleosomes in situ (inside cells) resembles the canonical structure determined in vitro 2…
View article: Evaluating the Impact of Continuing Medical Education in the Interdisciplinary Team: A Novel, Targeted Approach
Evaluating the Impact of Continuing Medical Education in the Interdisciplinary Team: A Novel, Targeted Approach Open
In order to maximise the learning potential of medical education programmes aimed at interdisciplinary or multidisciplinary teams, it is important to understand how the effectiveness of these programmes can vary between healthcare professi…
View article: Learning to automate cryo-electron microscopy data collection with Ptolemy.
Learning to automate cryo-electron microscopy data collection with Ptolemy. Open
Over the past decade, cryo-electron microscopy (cryoEM) has emerged as an important method for determining near-native, near-atomic resolution 3D structures of biological macromolecules. To meet the increasing demand for cryoEM, automated …
View article: Learning to automate cryo-electron microscopy data collection with <i>Ptolemy</i>
Learning to automate cryo-electron microscopy data collection with <i>Ptolemy</i> Open
Over the past decade, cryo-electron microscopy (cryoEM) has emerged as an important method for determining near-native, near-atomic resolution 3D structures of biological macromolecules. To meet the increasing demand for cryoEM, automated …
View article: Fully automated multi-grid cryoEM screening using <i>Smart Leginon</i>
Fully automated multi-grid cryoEM screening using <i>Smart Leginon</i> Open
Single-particle cryo-electron microscopy (cryoEM) is a swiftly growing method for understanding protein structure. With increasing demand for high-throughput, high-resolution cryoEM services comes greater demand for rapid and automated cry…
View article: Fully Automated Multi-Grid Cryo-EM Screening using Smart Leginon
Fully Automated Multi-Grid Cryo-EM Screening using Smart Leginon Open
Single particle cryo-electron microscopy (cryoEM) is a swiftly growing method for understanding protein structure. With increasing demand for high-throughput, high-resolution cryoEM services comes greater demand for rapid and automated cry…
View article: Better, Faster, Cheaper: Recent Advances in Cryo–Electron Microscopy
Better, Faster, Cheaper: Recent Advances in Cryo–Electron Microscopy Open
Cryo–electron microscopy (cryo-EM) continues its remarkable growth as a method for visualizing biological objects, which has been driven by advances across the entire pipeline. Developments in both single-particle analysis and in situ tomo…
View article: Architecture of the human erythrocyte ankyrin-1 complex
Architecture of the human erythrocyte ankyrin-1 complex Open
The stability and shape of the erythrocyte membrane is provided by the ankyrin-1 complex, but how it tethers the spectrin-actin cytoskeleton to the lipid bilayer and the nature of its association with the band 3 anion exchanger and the Rhe…
View article: In situ cryo-FIB/SEM Specimen Preparation Using the Waffle Method
In situ cryo-FIB/SEM Specimen Preparation Using the Waffle Method Open
Cryo-focused ion beam (FIB) milling of vitrified specimens is emerging as a powerful method for in situ specimen preparation. It allows for the preservation of native and near-native conditions in cells, and can reveal the molecular struct…
View article: Learning to automate cryo-electron microscopy data collection with Ptolemy
Learning to automate cryo-electron microscopy data collection with Ptolemy Open
Over the past decade, cryogenic electron microscopy (cryo-EM) has emerged as a primary method for determining near-native, near-atomic resolution 3D structures of biological macromolecules. In order to meet increasing demand for cryo-EM, a…