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View article: SARS-CoV-2 infection versus COVID-19 mRNA vaccination-induced Spike-specific memory B cells: frequencies, Ig class and subclass usage, cross-reactivities, and affinity distributions 9044
SARS-CoV-2 infection versus COVID-19 mRNA vaccination-induced Spike-specific memory B cells: frequencies, Ig class and subclass usage, cross-reactivities, and affinity distributions 9044 Open
Description Owing to differences in exposure route, memory B cells (Bmem) generated following infection of upper airway mucosa with SARS-CoV-2 virus or intramuscular injection of COVID-19 mRNA vaccine may differ in their Ig class/subclass …
View article: Optimizing PBMC utilization and cryopreservation for B cell ImmunoSpot® analysis 9043
Optimizing PBMC utilization and cryopreservation for B cell ImmunoSpot® analysis 9043 Open
Description Measuring frequencies of antigen-specific memory B cells (Bmem), their immunoglobulin (Ig) class and subclass usage, cross-reactivity and affinity can provide insights into the efficacy of future antibody responses in case of a…
View article: Immune response measurement of antigen-specific B cells by Elispot 2293
Immune response measurement of antigen-specific B cells by Elispot 2293 Open
Description The plasma and memory B-cell ELISPOT is an extremely sensitive assay based on the detection of antigen specific secreted antibodies from cryopreserved or fresh PBMCs. The B cell response encompasses four immunoglobulin (Ig) cla…
View article: SARS-CoV-2 Infection or COVID-19 mRNA Vaccination Elicits Partially Different Spike-Reactive Memory B Cell Responses in Naïve Individuals
SARS-CoV-2 Infection or COVID-19 mRNA Vaccination Elicits Partially Different Spike-Reactive Memory B Cell Responses in Naïve Individuals Open
Background: The COVID-19 pandemic provided a unique opportunity to evaluate how the human immune system responded to a novel pathogen and to determine whether immune responses initiated through natural infection differ from those elicited …
View article: SARS-CoV-2 Infection or COVID-19 mRNA Vaccination Elicit Similar Spike-Reactive Memory B Cell Responses in Naïve Individuals
SARS-CoV-2 Infection or COVID-19 mRNA Vaccination Elicit Similar Spike-Reactive Memory B Cell Responses in Naïve Individuals Open
Background: The COVID-19 pandemic provided a unique opportunity to evaluate how the human immune system responded to a novel pathogen, and to determine whether immune responses initiated through natural infection differ from those elicited…
View article: Optimizing PBMC Cryopreservation and Utilization for ImmunoSpot® Analysis of Antigen-Specific Memory B Cells
Optimizing PBMC Cryopreservation and Utilization for ImmunoSpot® Analysis of Antigen-Specific Memory B Cells Open
Background: Measuring frequencies of antigen-specific memory B cells (Bmem), their immunoglobulin (Ig) class and subclass usage, cross-reactivity, and affinity can provide insights into the efficacy of future antibody responses in case of …
View article: Optimizing PBMC Cryopreservation and Utilization for ImmunoSpot Analysis of Antigen-Specific Memory B Cells
Optimizing PBMC Cryopreservation and Utilization for ImmunoSpot Analysis of Antigen-Specific Memory B Cells Open
Background: Measuring frequencies of antigen-specific memory B cells (Bmem), their immunoglobulin (Ig) class and subclass usage, cross-reactivity and affinity can provide insights into the efficacy of future antibody responses in case of a…
View article: Theoretical and practical considerations for validating antigen-specific B cell ImmunoSpot assays
Theoretical and practical considerations for validating antigen-specific B cell ImmunoSpot assays Open
Owing to their ability to reliably detect even very rare antigen-specific B cells in cellular isolates such as peripheral blood mononuclear cells (PBMC), and doing so robustly in a high throughput-compatible manner, B cell ELISPOT/FluoroSp…
View article: Theoretical and practical considerations for validating antigen-specific B cell ImmunoSpot assays
Theoretical and practical considerations for validating antigen-specific B cell ImmunoSpot assays Open
Owing to their ability to reliably detect even very rare antigen-specific B cells in cellular isolates such as peripheral blood mononuclear cells (PBMC), and doing so robustly in a high throughput-compatible manner, B cell ELISPOT/FluoroSp…
View article: Four-Color ImmunoSpot® Assays Requiring Only 1–3 mL of Blood Permit Precise Frequency Measurements of Antigen-Specific B Cells-Secreting Immunoglobulins of All Four Classes and Subclasses
Four-Color ImmunoSpot® Assays Requiring Only 1–3 mL of Blood Permit Precise Frequency Measurements of Antigen-Specific B Cells-Secreting Immunoglobulins of All Four Classes and Subclasses Open
The B lymphocyte response can encompass four immunoglobulin (Ig) classes and four IgG subclasses, each contributing fundamentally different effector functions. Production of the appropriate Ig class/subclass is critical for both successful…
View article: Assessing the Affinity Spectrum of the Antigen-Specific B Cell Repertoire via ImmunoSpot®
Assessing the Affinity Spectrum of the Antigen-Specific B Cell Repertoire via ImmunoSpot® Open
The affinity distribution of the antigen-specific memory B cell (B mem ) repertoire in the body is a critical variable that defines an individual’s ability to rapidly generate high-affinity protective antibody specificities. Detailed measu…
View article: Monitoring Memory B Cells by Next-Generation ImmunoSpot® Provides Insights into Humoral Immunity that Measurements of Circulating Antibodies Do Not Reveal
Monitoring Memory B Cells by Next-Generation ImmunoSpot® Provides Insights into Humoral Immunity that Measurements of Circulating Antibodies Do Not Reveal Open
Memory B cells (B mem ) provide the second wall of adaptive humoral host defense upon specific antigen rechallenge when the first wall, consisting of preformed antibodies originating from a preceding antibody response, fails. This is the c…
View article: Monitoring memory B cells by next generation ImmunoSpot® provides insights into humoral immunity that measurements of circulating antibodies do not reveal
Monitoring memory B cells by next generation ImmunoSpot® provides insights into humoral immunity that measurements of circulating antibodies do not reveal Open
Memory B cells (B mem ) provide the second wall of adaptive humoral host defense upon specific antigen rechallenge when the first wall, consisting of pre-formed antibodies originating from a preceding antibody response, fails. This is the …
View article: Four-color ImmunoSpot® assays requiring only 1-3 mL of blood permit precise frequency measurements of antigen-specific B cells secreting immunoglobulins of all four classes and subclasses
Four-color ImmunoSpot® assays requiring only 1-3 mL of blood permit precise frequency measurements of antigen-specific B cells secreting immunoglobulins of all four classes and subclasses Open
The B lymphocyte response can encompass four immunoglobulin (Ig) classes and four IgG subclasses, each contributing fundamentally different effector functions. Production of the appropriate Ig class/subclass is critical for both successful…
View article: Assessing the affinity spectrum of the antigen-specific B cell repertoire via ImmunoSpot®
Assessing the affinity spectrum of the antigen-specific B cell repertoire via ImmunoSpot® Open
The affinity distribution of the antigen-specific memory B cell (B mem ) repertoire in the body is a critical variable that defines an individual’s ability to rapidly generate high affinity protective antibody specificities. Detailed measu…
View article: Four-color ImmunoSpot® assays requiring only 1-3 mL of blood permit precise frequency measurements of antigen-specific B cells secreting immunoglobulins of all four classes and subclasses
Four-color ImmunoSpot® assays requiring only 1-3 mL of blood permit precise frequency measurements of antigen-specific B cells secreting immunoglobulins of all four classes and subclasses Open
The B lymphocyte response can encompass four immunoglobulin (Ig) classes and four IgG subclasses, each contributing fundamentally different effector functions. Production of the appropriate Ig class/subclass is critical for both successful…
View article: Assessing the affinity spectrum of the antigen-specific B cell repertoire via ImmunoSpot®
Assessing the affinity spectrum of the antigen-specific B cell repertoire via ImmunoSpot® Open
The affinity distribution of the antigen-specific memory B cell (B mem ) repertoire in the body is a critical variable that defines an individual’s ability to rapidly generate high affinity protective antibody specificities. Detailed measu…
View article: Monitoring memory B cells by next generation ImmunoSpot® provides insights into humoral immunity that measurements of circulating antibodies do not reveal
Monitoring memory B cells by next generation ImmunoSpot® provides insights into humoral immunity that measurements of circulating antibodies do not reveal Open
Memory B cells (B mem ) provide the second wall of adaptive humoral host defense upon specific antigen rechallenge when the first wall, consisting of pre-formed antibodies originating from a preceding antibody response, fails. This is the …
View article: Antibody Levels Poorly Reflect on the Frequency of Memory B Cells Generated following SARS-CoV-2, Seasonal Influenza, or EBV Infection
Antibody Levels Poorly Reflect on the Frequency of Memory B Cells Generated following SARS-CoV-2, Seasonal Influenza, or EBV Infection Open
The scope of immune monitoring is to define the existence, magnitude, and quality of immune mechanisms operational in a host. In clinical trials and praxis, the assessment of humoral immunity is commonly confined to measurements of serum a…
View article: Antibody levels poorly reflect on the frequency of memory B cells generated following SARS-CoV-2, seasonal influenza, or EBV infection
Antibody levels poorly reflect on the frequency of memory B cells generated following SARS-CoV-2, seasonal influenza, or EBV infection Open
Assessment of humoral immunity is commonly confined to measurements of serum antibody reactivity. These pre-formed immunoglobulin molecules can convey immunity by preventing a re-infection. In some instances, however, a re-infection does o…
View article: Affinity Tag Coating Enables Reliable Detection of Antigen-Specific B Cells in Immunospot Assays
Affinity Tag Coating Enables Reliable Detection of Antigen-Specific B Cells in Immunospot Assays Open
Assessment of humoral immunity to SARS-CoV-2 and other infectious agents is typically restricted to detecting antigen-specific antibodies in the serum. Rarely does immune monitoring entail assessment of the memory B-cell compartment itself…
View article: Affinity tag coating enables reliable detection of antigen-specific B cells in ImmunoSpot assays
Affinity tag coating enables reliable detection of antigen-specific B cells in ImmunoSpot assays Open
Assessment of humoral immunity to SARS-CoV-2 and other infectious agents is typically restricted to detecting antigen-specific antibody in the serum. Rarely does immune monitoring entail assessment of the memory B cell compartment itself, …
View article: A New Method for Extracting Alkaloid Defenses from Poison Frog Skins
A New Method for Extracting Alkaloid Defenses from Poison Frog Skins Open
Poison frogs possess a diversity of skin alkaloids that serve as defense against predation and inhibit microbial infection. These alkaloid defenses are exclusively obtained from a diet of alkaloid-containing arthropods and differ significa…