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View article: Cellular and functional insights into FIH-mediated hydroxylation of TRPA1
Cellular and functional insights into FIH-mediated hydroxylation of TRPA1 Open
Transient receptor potential cation channel, subfamily A, member 1 (TRPA1), also known as transient receptor potential ankyrin 1, is an ion channel located on the plasma membrane of cells. It is best known as a sensor for pain, cold, and i…
View article: Enhancing Gene Delivery in NB-4 Cells: Overcoming Transduction and Selection Challenges
Enhancing Gene Delivery in NB-4 Cells: Overcoming Transduction and Selection Challenges Open
Efficient gene transduction and cell viability are critical factors in genetic manipulation for research and therapeutic purposes. In this study, we explored the challenges associated with transducing the NB-4 cell line, a well-established…
View article: Mechanisms of SARS-CoV-2 Inactivation Using UVC Laser Radiation
Mechanisms of SARS-CoV-2 Inactivation Using UVC Laser Radiation Open
Severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) has had a tremendous impact on humanity. Prevention of transmission by disinfection of surfaces and aerosols through a chemical-free method is highly desirable. Ultraviolet C (UV…
View article: Nuclear Phosphoinositides as Key Determinants of Nuclear Functions
Nuclear Phosphoinositides as Key Determinants of Nuclear Functions Open
Polyphosphoinositides (PPIns) are signalling messengers representing less than five per cent of the total phospholipid concentration within the cell. Despite their low concentration, these lipids are critical regulators of various cellular…
View article: A luminescence-based reporter to study tau secretion reveals overlapping mechanisms for the release of healthy and pathological tau
A luminescence-based reporter to study tau secretion reveals overlapping mechanisms for the release of healthy and pathological tau Open
In Alzheimer’s disease, tau pathology is thought to spread via a prion-like manner along connected neuronal networks. For this to occur, the usually cytosolic tau protein must be secreted via an unconventional mechanism prior to uptake int…
View article: Supplementary Figure 4 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression
Supplementary Figure 4 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression Open
PDF file - 60K, A. The gene expression profiles of PIP4K2B in normal and tumour tissues were extracted (GSE10780) and compared. PIP4K2B levels were significantly lower in tumor samples (P=0.04) B. Breast tumour samples from 17 data sets re…
View article: Supplementary Figure 7 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression
Supplementary Figure 7 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression Open
PDF file - 67K, Control or PIP4K2B knockdown MCF10A cells were labelled with 32P-orthophosphate for two hours after which lipids were extracted, deacetylated and the lipid head groups were separated by HPLC. Radioactivity was quantified us…
View article: Supplementary Figure 3 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression
Supplementary Figure 3 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression Open
PDF file - 274K, A. Expression profiles from 17 data sets representing 2999 tumors were extracted, normalised and hierarchically clustered according to tumour subtype. The heat map shows the relative transcript expression of genes in order…
View article: Supplementary Figure 7 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression
Supplementary Figure 7 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression Open
PDF file - 67K, Control or PIP4K2B knockdown MCF10A cells were labelled with 32P-orthophosphate for two hours after which lipids were extracted, deacetylated and the lipid head groups were separated by HPLC. Radioactivity was quantified us…
View article: Supplementary Figure 6 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression
Supplementary Figure 6 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression Open
PDF file - 46K, A. Lysates from MCF10A cells after 2 and 4 days of growth were analysed by western blotting using the antibodies shown. B. Lysates from cells treated as controls or with MG132 were analysed by western blotting with an anti-…
View article: Supplementary Figure 5 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression
Supplementary Figure 5 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression Open
PDF file - 89K, A. Gene expression profiles of widely used breast tumour cell lines were extracted from three different data sets, normalised and clustered into the designated molecular subtypes. The expression of PIP4K2A, 2B and 2C are gr…
View article: Supplementary Figure 6 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression
Supplementary Figure 6 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression Open
PDF file - 46K, A. Lysates from MCF10A cells after 2 and 4 days of growth were analysed by western blotting using the antibodies shown. B. Lysates from cells treated as controls or with MG132 were analysed by western blotting with an anti-…
View article: Supplementary Figure 1 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression
Supplementary Figure 1 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression Open
PDF file - 65K, A. Lysates from control (pLKO1) or PIP4k2B (shPIP4k2B) knockdown MCF10A cells were immunoblotted using the PIP4K2BP6 antibody. B. The PIP4K2BP6 antibody was immunodepleted by passing it through a column to which the P6 anti…
View article: Supplementary Figure 2 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression
Supplementary Figure 2 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression Open
PDF file - 383K, Cells as indicated were fixed and embedded in paraffin and sections were cut and mounted. The sections were stained with the PIP4K2BP6 antibody after antigen retrieval.
View article: Supplementary Figure 4 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression
Supplementary Figure 4 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression Open
PDF file - 60K, A. The gene expression profiles of PIP4K2B in normal and tumour tissues were extracted (GSE10780) and compared. PIP4K2B levels were significantly lower in tumor samples (P=0.04) B. Breast tumour samples from 17 data sets re…
View article: Data from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression
Data from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression Open
Phosphatidylinositol-5-phosphate (PtdIns5P) 4-kinase β (PIP4K2B) directly regulates the levels of two important phosphoinositide second messengers, PtdIns5P and phosphatidylinositol-(4,5)-bisphosphate [PtdIns(4,5)P2]. PIP4K2B has been link…
View article: Supplementary Figure 5 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression
Supplementary Figure 5 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression Open
PDF file - 89K, A. Gene expression profiles of widely used breast tumour cell lines were extracted from three different data sets, normalised and clustered into the designated molecular subtypes. The expression of PIP4K2A, 2B and 2C are gr…
View article: Supplementary Figure 2 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression
Supplementary Figure 2 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression Open
PDF file - 383K, Cells as indicated were fixed and embedded in paraffin and sections were cut and mounted. The sections were stained with the PIP4K2BP6 antibody after antigen retrieval.
View article: Supplementary Figure 3 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression
Supplementary Figure 3 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression Open
PDF file - 274K, A. Expression profiles from 17 data sets representing 2999 tumors were extracted, normalised and hierarchically clustered according to tumour subtype. The heat map shows the relative transcript expression of genes in order…
View article: Supplementary Figure Legend from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression
Supplementary Figure Legend from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression Open
PDF file - 77K
View article: Supplementary Figure 1 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression
Supplementary Figure 1 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression Open
PDF file - 65K, A. Lysates from control (pLKO1) or PIP4k2B (shPIP4k2B) knockdown MCF10A cells were immunoblotted using the PIP4K2BP6 antibody. B. The PIP4K2BP6 antibody was immunodepleted by passing it through a column to which the P6 anti…
View article: Supplementary Figure Legend from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression
Supplementary Figure Legend from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression Open
PDF file - 77K
View article: Data from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression
Data from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression Open
Phosphatidylinositol-5-phosphate (PtdIns5P) 4-kinase β (PIP4K2B) directly regulates the levels of two important phosphoinositide second messengers, PtdIns5P and phosphatidylinositol-(4,5)-bisphosphate [PtdIns(4,5)P2]. PIP4K2B has been link…
View article: Mechanisms of SARS-CoV-2 Inactivation using UVC Laser Radiation
Mechanisms of SARS-CoV-2 Inactivation using UVC Laser Radiation Open
Severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) has had a tremendous impact on humanity. Prevention of transmission by disinfection of surfaces and aerosols through a chemical-free method is highly desirable. Ultraviolet C (UV…
View article: PIP4K2B is a mechanosensor and induces heterochromatin-driven nuclear softening through UHRF1
PIP4K2B is a mechanosensor and induces heterochromatin-driven nuclear softening through UHRF1 Open
Phosphatidylinositol-5-phosphate (PtdIns5P)-4-kinases (PIP4Ks) are stress-regulated phosphoinositide kinases able to phosphorylate PtdIns5P to PtdIns(4, 5)P2. In cancer patients their expression is typically associated with bad prognosis. …
View article: Identification and optimization of a novel series of selective PIP5K inhibitors
Identification and optimization of a novel series of selective PIP5K inhibitors Open
Phosphatidyl inositol (4,5)-bisphosphate (PI(4,5)P2) plays several key roles in human biology and the lipid kinase that produces PI(4,5)P2, PIP5K, has been hypothesized to provide a potential therapeutic target of interest in the treatment…
View article: Proteomic characterization of GSK3β knockout shows altered cell adhesion and metabolic pathway utilisation in colorectal cancer cells
Proteomic characterization of GSK3β knockout shows altered cell adhesion and metabolic pathway utilisation in colorectal cancer cells Open
Glycogen-specific kinase (GSK3β) is an integral regulator of the Wnt signalling pathway as well as many other diverse signalling pathways and processes. Dys-regulation of GSK3β is implicated in many different pathologies, including neurode…
View article: PIP4Ks impact on PI3K, FOXP3, and UHRF1 signaling and modulate human regulatory T cell proliferation and immunosuppressive activity
PIP4Ks impact on PI3K, FOXP3, and UHRF1 signaling and modulate human regulatory T cell proliferation and immunosuppressive activity Open
Significance PIP4Ks are druggable lipid kinases critical in cancer biology whose function in human immunity remains unknown. Here, we show that PIP4Ks specifically control the growth and activity of a subset of human immune cells called Tr…
View article: “Modulating Phosphoinositide Profiles as a Roadmap for Treatment in Acute Myeloid Leukemia”
“Modulating Phosphoinositide Profiles as a Roadmap for Treatment in Acute Myeloid Leukemia” Open
Polyphosphoinositides (PPIns) and their modulating enzymes are involved in regulating many important cellular functions including proliferation, differentiation or gene expression, and their deregulation is involved in human diseases such …