Osvaldo Bogado Pascottini
YOU?
Author Swipe
View article: Inflammatory and metabolic markers in postpartum dairy cows developing reproductive tract inflammatory disease: A case-control study
Inflammatory and metabolic markers in postpartum dairy cows developing reproductive tract inflammatory disease: A case-control study Open
We investigated associations of early postpartum systemic inflammatory and metabolic markers in Holstein cows with different manifestations of reproductive tract inflammatory disease (RTID). A retrospective case-control study was conducted…
View article: Follicular fluid-derived small extracellular vesicles during individual in vitro maturation improve blastocyst development
Follicular fluid-derived small extracellular vesicles during individual in vitro maturation improve blastocyst development Open
Introduction We evaluated the impact of follicular fluid-derived small extracellular vesicles (FF-sEVs) supplementation during oocyte maturation in vitro on bovine embryo outcomes, comparing group and individual culture systems. Methods Fo…
View article: Functional and transcriptomic profiling of bovine circulating neutrophils during in vitro lipopolysaccharide challenge across different energetic states
Functional and transcriptomic profiling of bovine circulating neutrophils during in vitro lipopolysaccharide challenge across different energetic states Open
Polymorphonuclear leukocytes are pivotal players in the innate immune response of dairy cows. Their functionality (phagocytosis [PC] and oxidative burst [OB]) and gene expression patterns, however, can be modulated by varying energetic con…
View article: Artificial intelligence outperforms humans in morphology-based oocyte selection in cattle
Artificial intelligence outperforms humans in morphology-based oocyte selection in cattle Open
Evaluating cumulus-oocyte complex (COC) morphology is commonly used to assess oocyte quality. However, clear guidelines on interpreting COC morphology data are lacking as this evaluation method is subjective. In the present study, individu…
View article: Single-cell multiomic analysis reveals methylome and transcriptome deviations following oocyte maturation in vitro
Single-cell multiomic analysis reveals methylome and transcriptome deviations following oocyte maturation in vitro Open
In brief In vitro maturation is an essential tool in reproductive technologies, though its impact on oocyte quality remains a concern. This study shows that in vitro maturation alters gene expression and DNA methylation in bovine oocytes c…
View article: Review: Risks and benefits of introducing double-muscled Belgian Blue cattle to Indonesia’s tropical climate
Review: Risks and benefits of introducing double-muscled Belgian Blue cattle to Indonesia’s tropical climate Open
Residiwati G, Tuska HSA, Parlindungan O, Siswanti SW, Leemans B, Goossens K, Rochmi SE, Budiono, Budiyanto A, Purwantara B, Lonameo BK, Pascottini OB, Opsomer G, Van Soom A. 2025. Review: Risks and benefits of introducing double-muscled Be…
View article: Shotgun metagenomic composition, microbial interactions and functional insights into the uterine microbiome of postpartum dairy cows with clinical and subclinical endometritis
Shotgun metagenomic composition, microbial interactions and functional insights into the uterine microbiome of postpartum dairy cows with clinical and subclinical endometritis Open
View article: Tracing the dynamic changes in the lncRNA-mediated competing endogenous RNA network during bovine preimplantation embryo development
Tracing the dynamic changes in the lncRNA-mediated competing endogenous RNA network during bovine preimplantation embryo development Open
Long noncoding RNAs (lncRNAs) can regulate gene expression by "sponging" microRNAs (miRNAs), reducing their inhibitory effects on mRNAs. However, this mechanism has been minimally investigated in preimplantation embryo development. In this…
View article: Effect of elective cesarean section on the incidence of subclinical endometritis in Belgian Blue cows
Effect of elective cesarean section on the incidence of subclinical endometritis in Belgian Blue cows Open
Over 95% of Belgian Blue (BB) cows deliver via elective cesarean section (CS). The aim of this study was to determine the effect of elective CS on the incidence of subclinical endometritis (SCE) using cytobrush (CB) and low-volume lavage (…
View article: Fundamentals of microbiome-based therapies for reproductive tract inflammatory diseases in domestic animals
Fundamentals of microbiome-based therapies for reproductive tract inflammatory diseases in domestic animals Open
Reproductive tract inflammatory diseases (RTID) present significant health challenges in domestic animals, impacting welfare, fertility, and productivity. Traditionally, antibiotics have been the primary treatment for these conditions, how…
View article: Shortening of the telomere length during the transition period of dairy cows in relation to biological stress
Shortening of the telomere length during the transition period of dairy cows in relation to biological stress Open
Telomere length (TL) is a recognized biomarker for ageing in multiple species. In dairy cattle, the transition period is considered a very stressful period. We hypothesized that TL shortens during this period. Holstein cows (n = 61) were f…
View article: Oocyte holding and in vitro maturation duration between 28 and 34 hours do not affect equine OPU-ICSI outcomes
Oocyte holding and in vitro maturation duration between 28 and 34 hours do not affect equine OPU-ICSI outcomes Open
View article: Effect of type of anticoagulant, transportation time, and glucose in the culture media on neutrophil viability and function test results in dairy cattle
Effect of type of anticoagulant, transportation time, and glucose in the culture media on neutrophil viability and function test results in dairy cattle Open
In dairy cattle research, in vitro assessment of innate immune function is commonly evaluated by flow cytometry via the quantitative analysis of circulating polymorphonuclear leukocytes (PMN) functionalities specifically focusing on the ca…
View article: From follicle to blastocyst: microRNA-34c from follicular fluid-derived extracellular vesicles modulates blastocyst quality
From follicle to blastocyst: microRNA-34c from follicular fluid-derived extracellular vesicles modulates blastocyst quality Open
View article: How to beat the bull: Lycopene as a tool to improve in vitro fertilization efficiency in bulls with high polyspermy
How to beat the bull: Lycopene as a tool to improve in vitro fertilization efficiency in bulls with high polyspermy Open
View article: Lessons learned from bovine subclinical endometritis: a systematic review exploring its potential relevance to chronic endometritis in women
Lessons learned from bovine subclinical endometritis: a systematic review exploring its potential relevance to chronic endometritis in women Open
Chronic endometritis (CE) in humans is asymptomatic inflammation of the endometrium, associated with poor reproductive outcomes. Similarly, asymptomatic endometrial inflammation in cows, termed subclinical endometritis (SCE), is associated…
View article: The In Vitro Effects of Carprofen on Lipopolysaccharide-Induced Neutrophil Extracellular Trap Formation in Dairy Cows
The In Vitro Effects of Carprofen on Lipopolysaccharide-Induced Neutrophil Extracellular Trap Formation in Dairy Cows Open
The objective of this study was to develop an in vitro model that mimics inflammatory reactions and neutrophil extracellular traps (NETs) formation by polymorphonuclear leukocytes (PMNs) in dairy cows. This model was used to examine the ef…
View article: The Complex Interplay of Insulin Resistance and Metabolic Inflammation in Transition Dairy Cows
The Complex Interplay of Insulin Resistance and Metabolic Inflammation in Transition Dairy Cows Open
During the transition period, dairy cows exhibit heightened energy requirements to sustain fetal growth and lactogenesis. The mammary gland and the growing fetus increase their demand for glucose, leading to the mobilization of lipids to s…
View article: Oocyte Holding and in Vitro Maturation Duration between 28 and 34 Hours Do Not Affect Equine Opu-Icsi Outcomes
Oocyte Holding and in Vitro Maturation Duration between 28 and 34 Hours Do Not Affect Equine Opu-Icsi Outcomes Open
View article: IETS 2024 Editors and Reviewers
IETS 2024 Editors and Reviewers Open
View article: Manual versus deep learning measurements to evaluate cumulus expansion of bovine oocytes and its relationship with embryo development in vitro
Manual versus deep learning measurements to evaluate cumulus expansion of bovine oocytes and its relationship with embryo development in vitro Open
View article: Enhancing bovine abortion surveillance: A learning experience
Enhancing bovine abortion surveillance: A learning experience Open
Abortions and perinatal mortalities (APM) substantially affect cattle industry efficiency. Various infectious and noninfectious factors have been associated with bovine APM worldwide. Infections are often considered pivotal due to their ab…
View article: Genesis of clinical and subclinical endometritis in dairy cows
Genesis of clinical and subclinical endometritis in dairy cows Open
In brief Clinical and subclinical endometritis are different manifestations of reproductive tract inflammatory disease in dairy cows. This review addresses the genesis of clinical and subclinical endometritis considering metabolic stress, …
View article: General and comparative aspects of endometritis in domestic species: A review
General and comparative aspects of endometritis in domestic species: A review Open
Endometritis is a leading cause of sub‐ and infertility in domestic animal species. The healthy uterus is colonized by commensal bacteria, viruses and yeast/fungi that represent the nonpathogenic microbiota. A shift in the number or type o…
View article: Alternative Culture Systems for Bovine Oocyte In Vitro Maturation: Liquid Marbles and Differentially Shaped 96-Well Plates
Alternative Culture Systems for Bovine Oocyte In Vitro Maturation: Liquid Marbles and Differentially Shaped 96-Well Plates Open
In vivo-matured oocytes exhibit higher developmental competence than those matured in vitro but mimicking the in vivo environment by in vitro conditions has been challenging. Until now, conventional two-dimensional (2D) systems have been u…
View article: Transcriptomics Reveal Molecular Differences in Equine Oocytes Vitrified before and after In Vitro Maturation
Transcriptomics Reveal Molecular Differences in Equine Oocytes Vitrified before and after In Vitro Maturation Open
In the last decade, in vitro embryo production in horses has become an established clinical practice, but blastocyst rates from vitrified equine oocytes remain low. Cryopreservation impairs the oocyte developmental potential, which may be …
View article: Detection of <i>Anaplasma phagocytophilum</i> in fetal and placental tissue of bovine abortions and perinatal mortalities
Detection of <i>Anaplasma phagocytophilum</i> in fetal and placental tissue of bovine abortions and perinatal mortalities Open
Background Anaplasma phagocytophilum is a tick‐borne zoonotic bacterium that is the aetiologic pathogen of tick‐borne fever (TBF) in ruminants. In clinical bovine cases of TBF, abortion and stillbirth may be observed. However, in this rega…
View article: Cathepsin-L Secreted by High-Quality Bovine Embryos Exerts an Embryotrophic Effect In Vitro
Cathepsin-L Secreted by High-Quality Bovine Embryos Exerts an Embryotrophic Effect In Vitro Open
While human in vitro embryo production is generally performed individually, animal models have shown that culturing embryos in groups improves blastocyst yield and quality. Paracrine embryotrophins could be responsible for this improved em…
View article: Development of a Microfluidic Chip Powered by EWOD for In Vitro Manipulation of Bovine Embryos
Development of a Microfluidic Chip Powered by EWOD for In Vitro Manipulation of Bovine Embryos Open
Digital microfluidics (DMF) holds great potential for the alleviation of laboratory procedures in assisted reproductive technologies (ARTs). The electrowetting on dielectric (EWOD) technology provides dynamic culture conditions in vitro th…
View article: Embryo morphokinetics derived from fresh and vitrified bovine oocytes predict blastocyst development and nuclear abnormalities
Embryo morphokinetics derived from fresh and vitrified bovine oocytes predict blastocyst development and nuclear abnormalities Open
Embryo development is a dynamic process and critical stages may go unnoticed with the use of traditional morphologic assessments, especially the timing of embryonic divisions and aberrant zygotic cleavage patterns. Bovine embryo developmen…