Olaf Bergmann
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View article: Spatiotemporal gene expression and cellular dynamics of the developing human heart
Spatiotemporal gene expression and cellular dynamics of the developing human heart Open
Heart development relies on topologically orchestrated cellular transitions and interactions, many of which remain poorly characterized in humans. Here, we combined unbiased spatial and single-cell transcriptomics with imaging-based valida…
View article: A Latent Cardiomyocyte Regeneration Potential in Human Heart Disease
A Latent Cardiomyocyte Regeneration Potential in Human Heart Disease Open
BACKGROUND: Cardiomyocytes in the adult human heart show a regenerative capacity, with an annual renewal rate of ≈0.5%. Whether this regenerative capacity of human cardiomyocytes is employed in heart failure has been controversial. METHODS…
View article: Spatial Dynamics of the Developing Human Heart
Spatial Dynamics of the Developing Human Heart Open
Heart development relies on a topologically defined interplay between a diverse array of cardiac cells. We finely curated spatial and single-cell measurements with subcellular imaging-based transcriptomics validation to explore spatial dyn…
View article: Thioredoxin 2 protects mice against experimental myocardial infarction
Thioredoxin 2 protects mice against experimental myocardial infarction Open
Graphical abstract Experimental Myocardial infarction (MI) using ligation procedure induces cardiac dysfunction, high level of ROS, inflammation, apoptosis, fibrosis and cardiomyocyte (CM) loss. AAV overexpressing human Trx-2, specifically…
View article: Thioredoxin‐1 and its mimetic peptide improve systolic cardiac function and remodeling after myocardial infarction
Thioredoxin‐1 and its mimetic peptide improve systolic cardiac function and remodeling after myocardial infarction Open
Myocardial infarction (MI) is characterized by a significant loss of cardiomyocytes (CMs), and it is suggested that reactive oxygen species (ROS) are involved in cell cycle arrest, leading to impaired CM renewal. Thioredoxin‐1 (Trx‐1) scav…
View article: Dual spatially resolved transcriptomics for human host–pathogen colocalization studies in FFPE tissue sections
Dual spatially resolved transcriptomics for human host–pathogen colocalization studies in FFPE tissue sections Open
Technologies to study localized host–pathogen interactions are urgently needed. Here, we present a spatial transcriptomics approach to simultaneously capture host and pathogen transcriptome-wide spatial gene expression information from hum…
View article: Tracking cell turnover in human brain using 15N-thymidine imaging mass spectrometry
Tracking cell turnover in human brain using 15N-thymidine imaging mass spectrometry Open
Microcephaly is often caused by an impairment of the generation of neurons in the brain, a process referred to as neurogenesis. While most neurogenesis in mammals occurs during brain development, it thought to continue to take place throug…
View article: A latent cardiomyocyte regeneration potential in human heart disease
A latent cardiomyocyte regeneration potential in human heart disease Open
Cardiomyocytes in the adult human heart show a regenerative capacity, with an annual renewal rate around 0.5%. Whether this regenerative capacity of human cardiomyocytes is employed in heart failure has been controversial. Using retrospect…
View article: Spatially resolved multiomics on the neuronal effects induced by spaceflight
Spatially resolved multiomics on the neuronal effects induced by spaceflight Open
Impairment of the central nervous system (CNS) functions in astronauts is a major health risk for long-duration space missions. Here, for the first time, we combine single-cell multiomics (transcriptomics and chromatin accessibility) and s…
View article: FiNuTyper: an automated deep learning-based platform for simultaneous fiber and nucleus type analysis in human skeletal muscle
FiNuTyper: an automated deep learning-based platform for simultaneous fiber and nucleus type analysis in human skeletal muscle Open
Summary While manual quantification is still considered the gold standard for skeletal muscle histological analysis, it is time-consuming and prone to investigator bias. We assembled an automated image analysis pipeline, FiNuTyper (Fiber a…
View article: Diploid hepatocytes drive physiological liver renewal in adult humans
Diploid hepatocytes drive physiological liver renewal in adult humans Open
Physiological liver cell replacement is central to maintaining the organ's high metabolic activity, although its characteristics are difficult to study in humans. Using retrospective radiocarbon (14C) birth dating of cells, we report that …
View article: FUCCI-Based Live Imaging Platform Reveals Cell Cycle Dynamics and Identifies Pro-proliferative Compounds in Human iPSC-Derived Cardiomyocytes
FUCCI-Based Live Imaging Platform Reveals Cell Cycle Dynamics and Identifies Pro-proliferative Compounds in Human iPSC-Derived Cardiomyocytes Open
One of the major goals in cardiac regeneration research is to replace lost ventricular tissue with new cardiomyocytes. However, cardiomyocyte proliferation drops to low levels in neonatal hearts and is no longer efficient in compensating f…
View article: Dual spatially resolved transcriptomics for SARS-CoV-2 host-pathogen colocalization studies in humans
Dual spatially resolved transcriptomics for SARS-CoV-2 host-pathogen colocalization studies in humans Open
To advance our understanding of cellular host-pathogen interactions, technologies that facilitate the co-capture of both host and pathogen spatial transcriptome information are needed. Here, we present an approach to simultaneously capture…
View article: MSK-Mediated Phosphorylation of Histone H3 Ser28 Couples MAPK Signalling with Early Gene Induction and Cardiac Hypertrophy
MSK-Mediated Phosphorylation of Histone H3 Ser28 Couples MAPK Signalling with Early Gene Induction and Cardiac Hypertrophy Open
Heart failure is a leading cause of death that develops subsequent to deleterious hypertrophic cardiac remodelling. MAPK pathways play a key role in coordinating the induction of gene expression during hypertrophy. Induction of the immedia…
View article: FUCCI-based live imaging platform reveals cell cycle dynamics and identifies pro-proliferative compounds in human iPSC-derived cardiomyocytes
FUCCI-based live imaging platform reveals cell cycle dynamics and identifies pro-proliferative compounds in human iPSC-derived cardiomyocytes Open
One of the major goals in cardiac regeneration research is to replace lost ventricular tissue with new cardiomyocytes. However, cardiomyocyte proliferation drops to low levels in neonatal hearts and is no longer efficient in compensating f…
View article: Identification and characterization of distinct cell cycle stages in cardiomyocytes using the FUCCI transgenic system
Identification and characterization of distinct cell cycle stages in cardiomyocytes using the FUCCI transgenic system Open
Understanding the regulatory mechanism by which cardiomyocyte proliferation transitions to endoreplication and cell cycle arrest during the neonatal period is crucial for identifying proproliferative factors and developing regenerative the…
View article: Identification and characterization of distinct cell cycle stages in cardiomyocytes using the FUCCI transgenic system
Identification and characterization of distinct cell cycle stages in cardiomyocytes using the FUCCI transgenic system Open
Understanding the regulatory mechanism by which cardiomyocyte proliferation transitions to endoreplication and cell cycle arrest during the neonatal period is crucial for identifying proproliferative factors and developing regenerative the…
View article: Dynamics of Adipocyte Turnover in Humans
Dynamics of Adipocyte Turnover in Humans Open
Obesity is increasing in an epidemic fashion in most countries and constitutes a public health problem by enhancing the risk for cardiovascular disease and metabolic disorders such as type 2 diabetes. Owing to the increase in obesity, life…
View article: Inhibition of aquaporin-1 prevents myocardial remodeling by blocking the transmembrane transport of hydrogen peroxide
Inhibition of aquaporin-1 prevents myocardial remodeling by blocking the transmembrane transport of hydrogen peroxide Open
Pharmacological inhibition of AQP1 prevents H 2 O 2 transport and hypertrophy of cardiac myocytes.
View article: Diploid hepatocytes drive physiological liver renewal in adult humans
Diploid hepatocytes drive physiological liver renewal in adult humans Open
Summary Physiological liver cell replacement is central to maintaining the organ’s high metabolic activity, although its characteristics are difficult to study in humans. Using retrospective 14 C birth dating of cells, we report that human…
View article: MSK-mediated phosphorylation of Histone 3 Ser28 couples MAPK signaling with early gene induction and cardiac hypertrophy
MSK-mediated phosphorylation of Histone 3 Ser28 couples MAPK signaling with early gene induction and cardiac hypertrophy Open
Heart failure is a leading cause of death that develops subsequent to deleterious hypertrophic cardiac remodelling. MAPK pathways play a key role in coordinating the induction of gene expression during hypertrophy. Induction of the immedia…
View article: Printed elastic membranes for multimodal pacing and recording of human stem-cell-derived cardiomyocytes
Printed elastic membranes for multimodal pacing and recording of human stem-cell-derived cardiomyocytes Open
Bioelectronic interfaces employing arrays of sensors and bioactuators are promising tools for the study, repair and engineering of cardiac tissues. They are typically constructed from rigid and brittle materials processed in a cleanroom en…
View article: Genes Encoding ACE2, TMPRSS2 and Related Proteins Mediating SARS-CoV-2 Viral Entry are Upregulated with Age in Human Cardiomyocytes
Genes Encoding ACE2, TMPRSS2 and Related Proteins Mediating SARS-CoV-2 Viral Entry are Upregulated with Age in Human Cardiomyocytes Open
Age is an independent risk factor for adverse outcome in patients following COVID-19 infection. We hypothesised that differential expression of genes encoding proteins proposed to be required for entry of SARS-Cov-2 in aged compared to you…