Oliver M.T. Pearce
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View article: Multi-omics qualification of an organ-on-a-chip model of osteolytic bone metastasis
Multi-omics qualification of an organ-on-a-chip model of osteolytic bone metastasis Open
Bone is a primary site for metastasis in breast cancer, with up to 70 % of patients with metastatic breast cancer developing osteolytic bone lesions, wherein cancer cells drive osteoclast resorption of bone. However, progress in developing…
View article: Glycosylated extracellular matrix drives immune suppression by controlling T cell movement, macrophage phenotype, and macrophage-T cell crosstalk in triple negative breast cancer
Glycosylated extracellular matrix drives immune suppression by controlling T cell movement, macrophage phenotype, and macrophage-T cell crosstalk in triple negative breast cancer Open
The tumor extracellular matrix (ECM) is increasingly recognized as a key driver of immune suppression and therapy resistance in cancer. However, the specific ECM components and mechanisms that create this immunosuppressive environment rema…
View article: Programmable bacteria act as live biotherapeutics to remodel tumor stroma and potentiate immunotherapy
Programmable bacteria act as live biotherapeutics to remodel tumor stroma and potentiate immunotherapy Open
Immunotherapy has transformed cancer treatment but its efficacy is limited in many solid tumors in large part due to the immunosuppressive tumor microenvironment (TME). TME might be composed of a dense/stiff extracellular matrix (ECM) that…
View article: Ischaemic strokes and myocardial infarctions in a young male cannabis user
Ischaemic strokes and myocardial infarctions in a young male cannabis user Open
Ischaemic cerebral infarctions are seen in young people but, under the age of 30, multiple bilateral infarcts are uncommon; genetic pre-disposition and co-morbidities often underlie them. There is growing awareness of the potential impact …
View article: Supplementary Figure 7 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Supplementary Figure 7 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Supplementary Figure 7 shows representative images of each phenotype
View article: Data from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Data from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Immunotherapies for cancers of epithelial origin have limited efficacy, and a growing body of evidence links the composition of extracellular matrix (ECM) with the likelihood of a favorable response to treatment. The ECM may be considered …
View article: Supplementary Figure 1 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Supplementary Figure 1 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Supplementary Figure 1 shows how VCAN was enriched and mass spectrometry analysis of the sample
View article: FIGURE 2 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
FIGURE 2 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
VCAN is associated with T-cell localization. A, Matrix index from Pearce and colleagues (14). Arrows indicate upregulation or downregulation at gene (G) or protein (P) level. B, Level of gene expression of top five upregulated molecules in…
View article: Supplementary Figure 3 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Supplementary Figure 3 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Supplementary Figure 3 describes the transwell model
View article: FIGURE 1 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
FIGURE 1 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Determining the immune phenotype of tumor tissues. A total of 26 TNBC tissues were analyzed for the TIP. A, Annotation of slide on QuPath for analysis of the four regions: Stroma, Outer Invasive, Inner Invasive, and Tumor Core. Number of c…
View article: Supplementary Figure 5 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Supplementary Figure 5 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Supplementary Figure 5 outlines the analysis of matrix proteins in TMAs.
View article: TABLE 2 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
TABLE 2 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Primers for qRT-PCR. Primer sequences for VCAN isoforms and housekeeping gene RPS13
View article: FIGURE 4 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
FIGURE 4 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Expression of VCAN in tissues and cells. A, VCAN protein expression from IHC compared with gene expression from RNAscope. Spearman rank correlation. B, Comparison of FAP+ cells and percentage of α-SMA staining with number of VCAN+ cells. E…
View article: Supplementary Figure 5 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Supplementary Figure 5 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Supplementary Figure 5 outlines the analysis of matrix proteins in TMAs.
View article: FIGURE 1 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
FIGURE 1 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Determining the immune phenotype of tumor tissues. A total of 26 TNBC tissues were analyzed for the TIP. A, Annotation of slide on QuPath for analysis of the four regions: Stroma, Outer Invasive, Inner Invasive, and Tumor Core. Number of c…
View article: TABLE 1 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
TABLE 1 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Antibodies for IHC. Optimized parameters for each antibody
View article: Supplementary Figure 2 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Supplementary Figure 2 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Supplementary Figure 2 shows CS analysis from FFPE tissue sections
View article: Supplementary Figure 4 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Supplementary Figure 4 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Supplementary Figure 4 shows different method of TIP analysis and the TIPs from different subsets of tissues.
View article: TABLE 1 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
TABLE 1 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Antibodies for IHC. Optimized parameters for each antibody
View article: FIGURE 6 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
FIGURE 6 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
CS isomers expression in tissues. A, Structure of CS isoforms following digestion of the chains with ChABC. CS extracted from FFPE tissues in the epithelial zone and stromal zone. CS isoforms analyzed by MS to identify proportion of each i…
View article: FIGURE 7 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
FIGURE 7 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Effect of CS toward T-cell invasion. A, CS MS completed on the crude and enriched protein from HMF3S and HCC38 cell lines. CS isomers and A/C ratio determined. B, Transwell assay completed with 5 µg of each protein sample. Each sample was …
View article: Supplementary Figure 6 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Supplementary Figure 6 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Supplementary Figure 6 shows VCAN analysis in the TIPs from the second tissue subset
View article: FIGURE 4 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
FIGURE 4 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Expression of VCAN in tissues and cells. A, VCAN protein expression from IHC compared with gene expression from RNAscope. Spearman rank correlation. B, Comparison of FAP+ cells and percentage of α-SMA staining with number of VCAN+ cells. E…
View article: FIGURE 6 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
FIGURE 6 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
CS isomers expression in tissues. A, Structure of CS isoforms following digestion of the chains with ChABC. CS extracted from FFPE tissues in the epithelial zone and stromal zone. CS isoforms analyzed by MS to identify proportion of each i…
View article: FIGURE 2 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
FIGURE 2 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
VCAN is associated with T-cell localization. A, Matrix index from Pearce and colleagues (14). Arrows indicate upregulation or downregulation at gene (G) or protein (P) level. B, Level of gene expression of top five upregulated molecules in…
View article: Supplementary Figure 8 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Supplementary Figure 8 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Supplementary Figure 8 summarises the results identified.
View article: TABLE 2 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
TABLE 2 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Primers for qRT-PCR. Primer sequences for VCAN isoforms and housekeeping gene RPS13
View article: Supplementary Figure 4 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate
Supplementary Figure 4 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate Open
Supplementary Figure 4 shows different method of TIP analysis and the TIPs from different subsets of tissues.