Olivier Tytgat
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View article: Potential of Proteomics in Forensic Phenotyping: A Focus on Biological Sex Estimation
Potential of Proteomics in Forensic Phenotyping: A Focus on Biological Sex Estimation Open
Forensic DNA analysis is well established for phenotyping, providing valuable investigative leads. Proteomics, the large-scale study of proteins, is emerging as a complementary tool to DNA analysis, particularly for enhancing the evidentia…
View article: Recombinase Polymerase Amplification of Forensic Short Tandem Repeat Loci
Recombinase Polymerase Amplification of Forensic Short Tandem Repeat Loci Open
Short tandem repeats (STRs) are highly polymorphic repetitive DNA sequences extensively used in forensic science for identification of individuals. STR genotyping is usually performed by capillary electrophoresis (CE) or next-generation se…
View article: High-throughput single-cell screening of viable hybridomas and patient-derived antibody-secreting cells using punchable microwells
High-throughput single-cell screening of viable hybridomas and patient-derived antibody-secreting cells using punchable microwells Open
Monoclonal antibodies (mAbs) hold significant potential as therapeutic agents and are invaluable tools in biomedical research. However, the lack of efficient high-throughput screening methods for single antibody-secreting cells (ASCs) has …
View article: Cas9 targeted nanopore sequencing with enhanced variant calling improves CYP2D6-CYP2D7 hybrid allele genotyping
Cas9 targeted nanopore sequencing with enhanced variant calling improves CYP2D6-CYP2D7 hybrid allele genotyping Open
CYP2D6 is a very important pharmacogene as it is responsible for the metabolization or bioactivation of 20 to 30% of the clinically used drugs. However, despite its relatively small length of only 4.4 kb, it is one of the most challenging …
View article: Enrichment of circulating trophoblasts from maternal blood using filtration-based Metacell® technology
Enrichment of circulating trophoblasts from maternal blood using filtration-based Metacell® technology Open
In a cell-based non-invasive prenatal test (cbNIPT), intact circulating trophoblasts (CTs) are isolated from maternal blood for subsequent genetic analysis. Enrichment of these CTs from maternal blood is the most challenging step in the cb…
View article: Cas9 targeted nanopore sequencing with enhanced variant calling improves <i>CYP2D6</i>-<i>CYP2D7</i> hybrid allele genotyping
Cas9 targeted nanopore sequencing with enhanced variant calling improves <i>CYP2D6</i>-<i>CYP2D7</i> hybrid allele genotyping Open
CYP2D6 is one of the most challenging pharmacogenes to genotype due to the high similarity with its neighboring pseudogenes and the frequent occurrence of CYP2D6-CYP2D7 hybrids. Unfortunately, most current genotyping methods are therefore …
View article: Cov-MS: A Community-Based Template Assay for Mass-Spectrometry-Based Protein Detection in SARS-CoV-2 Patients
Cov-MS: A Community-Based Template Assay for Mass-Spectrometry-Based Protein Detection in SARS-CoV-2 Patients Open
Rising population density and global mobility are among the reasons why pathogens such as SARS-CoV-2, the virus that causes COVID-19, spread so rapidly across the globe. The policy response to such pandemics will always have to include acc…
View article: Digital Polymerase Chain Reaction for Assessment of Mutant Mitochondrial Carry-over after Nuclear Transfer for In Vitro Fertilization
Digital Polymerase Chain Reaction for Assessment of Mutant Mitochondrial Carry-over after Nuclear Transfer for In Vitro Fertilization Open
Background The quantification of mitochondrial DNA heteroplasmy for the diagnosis of mitochondrial disease or after mitochondrial donation, is performed mainly using next-generation sequencing strategies (NGS). Digital PCR (dPCR) has the p…
View article: Enrichment of circulating trophoblasts from maternal blood using laminar microscale vortices
Enrichment of circulating trophoblasts from maternal blood using laminar microscale vortices Open
Objective Enrichment of circulating trophoblasts (CTs) from maternal blood at week 11–13 of gestation, using laminar microscale vortices, and evaluation of the performance of the VTX‐1 Liquid Biopsy System in terms of CT recovery and purit…
View article: Cov-MS: a community-based template assay for clinical MS-based protein detection in SARS-CoV-2 patients
Cov-MS: a community-based template assay for clinical MS-based protein detection in SARS-CoV-2 patients Open
Rising population density and global mobility are among the reasons why pathogens such as SARS-CoV-2, the virus that causes COVID-19, spread so rapidly across the globe. The policy response to such pandemics will always have to include acc…
View article: Nanopore Sequencing of a Forensic STR Multiplex Reveals Loci Suitable for Single-Contributor STR Profiling
Nanopore Sequencing of a Forensic STR Multiplex Reveals Loci Suitable for Single-Contributor STR Profiling Open
Nanopore sequencing for forensic short tandem repeats (STR) genotyping comes with the advantages associated with massively parallel sequencing (MPS) without the need for a high up-front device cost, but genotyping is inaccurate, partially …
View article: Forensic STR profiling using Oxford Nanopore Technologies’ MinION sequencer
Forensic STR profiling using Oxford Nanopore Technologies’ MinION sequencer Open
Forensic STR profiling using massively parallel sequencing (MPS) has gained much attention as an alternative for the traditional capillary electrophoresis (CE) methods. Oxford Nanopore Technologies recently developed the ‘MinION’, a pocket…