Hemoglobin is the iron containing protein in red blood cells which carries oxygen from lungs to rest of the body tissues.Accurate measurement of hemoglobin is essential for diagnosing anemia, a condition characterized by a deficiency of red blood cells. This measurement is particularly vital before initiating blood transfusions for thalassemia patients.Non-invasive estimation of hemoglobin levels can be achieved through photoplethysmography (PPG)-based methods.PPG is an optical method to measure blood volume chang…

There are still a lot of real-world issues where the observed facts cannot effectively fit into frequently used classical probability models. To solve this, it is imperative to provide probability models that accurately represent the behavior of certain real-world phenomena. having considered these problems, the study proposed a new lifetime distribution, the Modified Dhillon Distribution (MDD), developed using the Beta integrated model approach. The study examines the statistical properties of the new distributio…

Abstract Multi-scale models integrating biomolecular data from genetic, transcriptional, and translational levels, coupled with extracellular microenvironments can assist in decoding the complex mechanisms underlying system-level diseases such as cancer. To investigate the emergent properties and clinical translation of such cancer models, we present Theatre for in silico Systems Oncology (TISON, https://tison.lums.edu.pk ), a next-generation web-based multi-scale modeling and simulation platform for in silico sys…

Zebrafish ( Danio rerio) is an important vertebrate model organism in biomedical research, especially suitable for morphological screening due to its transparent body during early development. Deep learning has emerged as a dominant paradigm for data analysis and found a number of applications in computer vision and image analysis. Here we demonstrate the potential of a deep learning approach for accurate high-throughput classification of whole-body zebrafish deformations in multifish microwell plates. Deep learni…

Quantitative microscopy deals with the extraction of quantitative measurements from samples observed under a microscope. Recent developments in microscopy systems, sample preparation and handling techniques have enabled high throughput biological experiments resulting in large amounts of image data, at biological scales ranging from subcellular structures such as fluorescently tagged nucleic acid sequences to whole organisms such as zebrafish embryos. Consequently, methods and algorithms for automated quantitative…