Ondřej Vaněk
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View article: Engineering a Functional Histidine Brace Copper-Binding Site into a <i>De Novo</i> -Designed Protein Scaffold
Engineering a Functional Histidine Brace Copper-Binding Site into a <i>De Novo</i> -Designed Protein Scaffold Open
De novo metalloprotein design has contributed to tremendous advances in bioinorganic chemistry by allowing the manufacturing of proteins with unique structures and functionalities that go beyond evolutionary constraints. Among the a…
View article: <i>Fusobacterium nucleatum</i> Lipopolysaccharides O-Antigen Defines a Novel Siglec-7 Binding Epitope
<i>Fusobacterium nucleatum</i> Lipopolysaccharides O-Antigen Defines a Novel Siglec-7 Binding Epitope Open
Fusobacterium nucleatum (Fn) is a Gram-negative bacterium predominantly found in the human oral cavity, occasionally linked to systemic diseases, including colorectal cancer. Bacterial lipopolysaccharides (LPSs) represent one…
View article: Molecular Basis of Siglec-7 Recognition by <i>Neisseria meningitidis</i> Serogroup Y CPS: Implications for Immune Evasion
Molecular Basis of Siglec-7 Recognition by <i>Neisseria meningitidis</i> Serogroup Y CPS: Implications for Immune Evasion Open
Siglecs, sialic-acid-binding immunoglobulin-like lectins, are key immune cell receptors that recognize sialic acid residues on cell surfaces. Pathogens and tumor cells exploit Siglecs to evade immune responses and modulate immunity, contri…
View article: Insights into Siglec‐7 Binding to Gangliosides: NMR Protein Assignment and the Impact of Ligand Flexibility
Insights into Siglec‐7 Binding to Gangliosides: NMR Protein Assignment and the Impact of Ligand Flexibility Open
Gangliosides, sialylated glycosphingolipids abundant in the nervous system, play crucial roles in neurotransmission, interaction with regulatory proteins, cell–cell recognition, and signaling. Altered gangliosides expression has been corre…
View article: Engineered cytokine/antibody fusion proteins improve IL-2 delivery to pro-inflammatory cells and promote antitumor activity
Engineered cytokine/antibody fusion proteins improve IL-2 delivery to pro-inflammatory cells and promote antitumor activity Open
Progress in cytokine engineering is driving therapeutic translation by overcoming these proteins' limitations as drugs. The IL-2 cytokine is a promising immune stimulant for cancer treatment but is limited by its concurrent activation of b…
View article: Tumor Carbohydrate Associated Antigen Analogs as Potential Binders for Siglec‐7
Tumor Carbohydrate Associated Antigen Analogs as Potential Binders for Siglec‐7 Open
We investigated two recently synthesized and characterized sialyl derivatives, bearing the Neu5Ac‐α‐(2‐6)‐Gal epitope, as promising binders for Siglec‐7, an inhibitory Siglec mainly found on natural killer cells. A variety of sialoglycan s…
View article: Engineered cytokine/antibody fusion proteins improve delivery of IL-2 to pro-inflammatory cells and promote antitumor activity
Engineered cytokine/antibody fusion proteins improve delivery of IL-2 to pro-inflammatory cells and promote antitumor activity Open
Progress in cytokine engineering is driving therapeutic translation by overcoming the inherent limitations of these proteins as drugs. The interleukin-2 (IL-2) cytokine harbors great promise as an immune stimulant for cancer treatment. How…
View article: Structure of the human NK cell NKR-P1:LLT1 receptor:ligand complex reveals clustering in the immune synapse
Structure of the human NK cell NKR-P1:LLT1 receptor:ligand complex reveals clustering in the immune synapse Open
View article: Protein purification strategies must consider downstream applications and individual biological characteristics
Protein purification strategies must consider downstream applications and individual biological characteristics Open
View article: Tumor Marker B7-H6 Bound to the Coiled Coil Peptide-Polymer Conjugate Enables Targeted Therapy by Activating Human Natural Killer Cells
Tumor Marker B7-H6 Bound to the Coiled Coil Peptide-Polymer Conjugate Enables Targeted Therapy by Activating Human Natural Killer Cells Open
Targeted cancer immunotherapy is a promising tool for restoring immune surveillance and eradicating cancer cells. Hydrophilic polymers modified with coiled coil peptide tags can be used as universal carriers designed for cell-specific deli…
View article: Correction to: Reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study
Correction to: Reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study Open
View article: Influence of glycosylation on the structure of human natural killer cell receptor NKp30 in complex with its tumour ligand B7-H6
Influence of glycosylation on the structure of human natural killer cell receptor NKp30 in complex with its tumour ligand B7-H6 Open
NKp30 is an activating receptor on the surface of human natural killer (NK) cells.Its crystal structure has been published previously by Joyce et al. [1], PDB code 3NOI.B7-H6 is an activating immunoligand expressed by some tumor cells.Its …
View article: Crystal structure of the human NKR-P1 bound to its lymphocyte ligand LLT1 reveals receptor clustering in the immune synapse
Crystal structure of the human NKR-P1 bound to its lymphocyte ligand LLT1 reveals receptor clustering in the immune synapse Open
Human NKR-P1 (CD161, KLRB1 ) and its ligand LLT1 ( CLEC2D ) are a prototypical inhibitory C-type lectin-like receptor:ligand pair of NK cells with a critical role in homing lymphocytes to immune-privileged sites, particularly in multiple s…
View article: The order of <scp>PDZ3</scp> and <scp>TrpCage</scp> in fusion chimeras determines their properties—a biophysical characterization
The order of <span>PDZ3</span> and <span>TrpCage</span> in fusion chimeras determines their properties—a biophysical characterization Open
Most of the structural proteins known today are composed of domains that carry their own functions while keeping their structural properties. It is supposed that such domains, when taken out of the context of the whole protein, can retain …
View article: Recent advances on smart glycoconjugate vaccines in infections and cancer
Recent advances on smart glycoconjugate vaccines in infections and cancer Open
Vaccination is one of the greatest achievements in biomedical research preventing death and morbidity in many infectious diseases through the induction of pathogen‐specific humoral and cellular immune responses. Currently, no effective vac…
View article: Reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study
Reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study Open
View article: Community-Wide Experimental Evaluation of the PROSS Stability-Design Method
Community-Wide Experimental Evaluation of the PROSS Stability-Design Method Open
View article: X-Ray Diffraction data from Extracellular domain of mouse NKR-P1A, source of 3M9Z structure
X-Ray Diffraction data from Extracellular domain of mouse NKR-P1A, source of 3M9Z structure Open
native data set
View article: Emerging glyco‐based strategies to steer immune responses
Emerging glyco‐based strategies to steer immune responses Open
Glycan structures are common posttranslational modifications of proteins, which serve multiple important structural roles (for instance in protein folding), but also are crucial participants in cell–cell communications and in the regulatio…
View article: Size Effects on Surface Chemistry and Raman Spectra of Sub-5 nm Oxidized High-Pressure High-Temperature and Detonation Nanodiamonds
Size Effects on Surface Chemistry and Raman Spectra of Sub-5 nm Oxidized High-Pressure High-Temperature and Detonation Nanodiamonds Open
International audience
View article: Characterization of AMBN I and II Isoforms and Study of Their Ca2+-Binding Properties
Characterization of AMBN I and II Isoforms and Study of Their Ca2+-Binding Properties Open
Ameloblastin (Ambn) as an intrinsically disordered protein (IDP) stands for an important role in the formation of enamel—the hardest biomineralized tissue commonly formed in vertebrates. The human ameloblastin (AMBN) is expressed in two is…
View article: Phlebotomus perniciosus Recombinant Salivary Proteins Polarize Murine Macrophages Toward the Anti-Inflammatory Phenotype
Phlebotomus perniciosus Recombinant Salivary Proteins Polarize Murine Macrophages Toward the Anti-Inflammatory Phenotype Open
Phlebotomus perniciosus (Diptera: Phlebotominae) is a medically and veterinary important insect vector. It transmits the unicellular parasite Leishmania infantum that multiplies intracellularly in macrophages causing life-thr…
View article: Natural Killer Cell Activation Receptor NKp30 Oligomerization Depends on Its N-Glycosylation
Natural Killer Cell Activation Receptor NKp30 Oligomerization Depends on Its N-Glycosylation Open
NKp30 is one of the main human natural killer (NK) cell activating receptors used in directed immunotherapy. The oligomerization of the NKp30 ligand binding domain depends on the length of the C-terminal stalk region, but our structural kn…
View article: Publisher Correction: Production of recombinant soluble dimeric C-type lectin-like receptors of rat natural killer cells
Publisher Correction: Production of recombinant soluble dimeric C-type lectin-like receptors of rat natural killer cells Open
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
View article: X-Ray Diffraction data from NKp30 in complex with B7H6, source of 6YJP structure
X-Ray Diffraction data from NKp30 in complex with B7H6, source of 6YJP structure Open
six data sets (six crystals) used for merging and molecular replacement (no experimental phasing)
View article: Molecular Mechanisms of the Interactions of N-(2-Hydroxypropyl)methacrylamide Copolymers Designed for Cancer Therapy with Blood Plasma Proteins
Molecular Mechanisms of the Interactions of N-(2-Hydroxypropyl)methacrylamide Copolymers Designed for Cancer Therapy with Blood Plasma Proteins Open
The binding of plasma proteins to a drug carrier alters the circulation of nanoparticles (NPs) in the bloodstream, and, as a consequence, the anticancer efficiency of the entire nanoparticle drug delivery system. We investigate the possibl…
View article: Production of recombinant soluble dimeric C-type lectin-like receptors of rat natural killer cells
Production of recombinant soluble dimeric C-type lectin-like receptors of rat natural killer cells Open
Field study of the improved rapid sand fly exposure test in areas endemic for canine leishmaniasis Open
View article: Amine-binding properties of salivary yellow-related proteins in phlebotomine sand flies
Amine-binding properties of salivary yellow-related proteins in phlebotomine sand flies Open
The amine-binding properties of sand fly salivary yellow-related proteins (YRPs) were described only in Lutzomyia longipalpis sand flies. Here, we experimentally confirmed the kratagonist function of YRPs in the genus Phlebotomus. We utili…
View article: X-Ray Diffraction data from Dimeric form of human LLT1, a ligand for NKR-P1, source of 4QKH structure
X-Ray Diffraction data from Dimeric form of human LLT1, a ligand for NKR-P1, source of 4QKH structure Open
X-Ray Diffraction data from dimeric form of human LLT1, a ligand for NKR-P1, source of 4QKH structure