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View article: The Golgi Rim is a Precise Tetraplex of Golgin Proteins that Can Self-Assemble into Filamentous Bands
The Golgi Rim is a Precise Tetraplex of Golgin Proteins that Can Self-Assemble into Filamentous Bands Open
Golgin proteins have long been suspected to be organizers of the Golgi stack. Using three-dimensional super-resolution microscopy, we comprehensively localize the human golgin family at the rim of the Golgi apparatus at 10-20 nm resolution…
View article: Chromatin expansion microscopy reveals nanoscale organization of transcription and chromatin
Chromatin expansion microscopy reveals nanoscale organization of transcription and chromatin Open
Nanoscale chromatin organization regulates gene expression. Although chromatin is notably reprogrammed during zygotic genome activation (ZGA), the organization of chromatin regulatory factors during this universal process remains unclear. …
Quantifying Intracellular Nanoparticle Distributions with Three-Dimensional Super-Resolution Microscopy Open
Super-resolution microscopy can transform our understanding of nanoparticle-cell interactions. Here, we established a super-resolution imaging technology to visualize nanoparticle distributions inside mammalian cells. The cells were expose…
Unclearing Microscopy Open
The spatial resolution and contrast sensitivity of the human eye is limited, restricting our ability to directly see subcellular structures. We report a new principle for unaided eye cellular visualization in a method we call Unclearing Mi…
View article: All-optical visualization of specific molecules in the ultrastructural context of brain tissue
All-optical visualization of specific molecules in the ultrastructural context of brain tissue Open
Summary Understanding the molecular anatomy and neural connectivity of the brain requires imaging technologies that can map the 3D nanoscale distribution of specific proteins in the context of brain ultrastructure. Light and electron micro…
View article: Isotropic Imaging Across Spatial Scales with Axially Swept Light-Sheet Microscopy
Isotropic Imaging Across Spatial Scales with Axially Swept Light-Sheet Microscopy Open
Light-Sheet Fluorescence Microscopy (LSFM) is a rapidly growing technique that has gained tremendous popularity in the life sciences owing to its high-spatiotemporal resolution and gentle, non-phototoxic illumination. In this protocol, we …
View article: Supplementary document for 3D super-resolution deep-tissue imaging in living mice - 5109575.pdf
Supplementary document for 3D super-resolution deep-tissue imaging in living mice - 5109575.pdf Open
Supplementary Material
Light microscopy of proteins in their ultrastructural context Open
Resolving the distribution of specific proteins at the nanoscale in the structural context of the cell is a major challenge in fluorescence microscopy. Here we present a new concept that decrowds the intracellular space through 13 to 21-fo…
View article: 3D super-resolution deep-tissue imaging in living mice
3D super-resolution deep-tissue imaging in living mice Open
Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering unique insights into their organization. However, 3D-STED imaging deep inside biolog…