Oto Hanuš
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View article: Lactoferrin content determined in bovine milk by HPLC and mid-infrared spectrometry - Relation to udder health and potential for detection of milk adulteration
Lactoferrin content determined in bovine milk by HPLC and mid-infrared spectrometry - Relation to udder health and potential for detection of milk adulteration Open
Lactoferrin (LF) is a multifunctional glycoprotein linked to udder health in dairy cows. This study aimed to develop a calibration model for LF quantification using mid-infrared spectrometry (MIR-FT), with ion-pair reversed-phase high-perf…
View article: The effect of artificial reduction of the somatic cell count, as a violation of authenticity, on milk quality indicators
The effect of artificial reduction of the somatic cell count, as a violation of authenticity, on milk quality indicators Open
The somatic cell count (SCC) in raw milk is an important indicator of health and hygienic quality. Artificial reduction of the SCC (ARSCC) in milk, for the apparent improvement of milk quality for commercial reasons, is an undesirable phen…
View article: Screening Methods for Antimicrobial Residues in the Dairy Chain—The Past and the Present
Screening Methods for Antimicrobial Residues in the Dairy Chain—The Past and the Present Open
The presence of residues of antimicrobial substances in milk has been an important hygienic and technological parameter of raw milk quality since the 1960s. The presented review focuses on screening methods (microbiological inhibition meth…
View article: Dairy Chain Safety in the Context of Antibiotic Residues—Current Status of Confirmatory Liquid Chromatography Methods: A Review
Dairy Chain Safety in the Context of Antibiotic Residues—Current Status of Confirmatory Liquid Chromatography Methods: A Review Open
With ever-developing analytical capabilities, the protection of the dairy chain from contamination by residues of veterinary drugs is improving. Legislative requirements are an inherent part of this process. Of antibiotics in dairy farming…
View article: The stability of fatty acids in yoghurts produced from bulk milk samples intentionally selected according to dairy production systems
The stability of fatty acids in yoghurts produced from bulk milk samples intentionally selected according to dairy production systems Open
The fatty acid (FA) composition of milk fat can be positively influenced by the modification of dairy farming and the possible selection of raw material for processing. The question arises as to whether the benefits of a better FA composit…
View article: Relationships between milk ketone bodies and selected milk indicators during conventional and extended lactation
Relationships between milk ketone bodies and selected milk indicators during conventional and extended lactation Open
During lactation, dairy cows undergo various metabolic changes that are reflected not only in milk yield but also in milk composition. This study aimed to determine the milk performance and composition depending on the stage of lactation a…
View article: CSN1S1 and LALBA Polymorphisms and Other Factors Influencing Yield, Composition, Somatic Cell Score, and Technological Properties of Cow’s Milk
CSN1S1 and LALBA Polymorphisms and Other Factors Influencing Yield, Composition, Somatic Cell Score, and Technological Properties of Cow’s Milk Open
We evaluated the influence of CSN1S1 and LALBA polymorphisms on cow’s milk yield and quality. The analysis was done on Czech Simmental and Holstein cows. Non-genetic factors were included as well. CSN1S1 did not influence the milk performa…
View article: The interaction between the milk production, milk components with a low frequency of analysis and factors affecting the milk composition in dual-purpose Simmental cows
The interaction between the milk production, milk components with a low frequency of analysis and factors affecting the milk composition in dual-purpose Simmental cows Open
The aim of this study was to improve the understanding of the contribution of components typically found in milk, but which are not often included in analyses, to the nutritional status of dairy cows. This was undertaken by analysing the a…
View article: Use of MALDI-TOF MS technology to evaluate adulteration of small ruminant milk with raw bovine milk
Use of MALDI-TOF MS technology to evaluate adulteration of small ruminant milk with raw bovine milk Open
Detection of adulteration of small ruminant milk is very important for health and commercial reasons. New analytical and cost-effective methods need to be developed to detect new adulteration practices. In this work, we aimed to explore th…
View article: Silages from legume–cereal mixtures as a factor of dairy cow milk quality
Silages from legume–cereal mixtures as a factor of dairy cow milk quality Open
Currently, the prices of dairy cows feed with protein concentrates are rising and the climate change is also manifesting itself with a growing drought in animal production. These are the reasons why there is an increasing interest in growi…
View article: Supplementary files - Evaluation of MALDI-TOF MS technology in small ruminant milk adulteration using raw bovine milk
Supplementary files - Evaluation of MALDI-TOF MS technology in small ruminant milk adulteration using raw bovine milk Open
The dataset is a part of Supplementary file for the manuscript: Evaluation of MALDI-TOF MS technology in small ruminant milk adulteration using raw bovine milk by L. Rysova, P. Cejnar, O. Hanus, V. Legarova, J. Havlik, H. Nejeschlebova, I.…
View article: Supplementary files - Evaluation of MALDI-TOF MS technology in small ruminant milk adulteration using raw bovine milk
Supplementary files - Evaluation of MALDI-TOF MS technology in small ruminant milk adulteration using raw bovine milk Open
The dataset is a part of Supplementary file for the manuscript: Evaluation of MALDI-TOF MS technology in small ruminant milk adulteration using raw bovine milk by L. Rysova, P. Cejnar, O. Hanus, V. Legarova, J. Havlik, H. Nejeschlebova, I.…
View article: Supplementary files - Evaluation of MALDI-TOF MS technology in small ruminant milk adulteration using raw bovine milk
Supplementary files - Evaluation of MALDI-TOF MS technology in small ruminant milk adulteration using raw bovine milk Open
The dataset is a part of Supplementary file for the manuscript: Evaluation of MALDI-TOF MS technology in small ruminant milk adulteration using raw bovine milk by L. Rysova, P. Cejnar, O. Hanus, V. Legarova, J. Havlik, H. Nejeschlebova, I.…
View article: MALDI-TOF spectra of bovine, caprine and ovine milk and their mixtures
MALDI-TOF spectra of bovine, caprine and ovine milk and their mixtures Open
In this dataset we provide MALDI-TOF spectra of bovine milk in caprine and ovine milk. The dataset contains normalized spectra of pure milk samples and their mixtures. Different level of adulteration (0.5%, 1%, 5%, 10%, 20%, 40%, 60%, 80%)…
View article: MALDI-TOF spectra of bovine, caprine and ovine milk and their mixtures
MALDI-TOF spectra of bovine, caprine and ovine milk and their mixtures Open
In this dataset we provide MALDI-TOF spectra of bovine milk in caprine and ovine milk. The dataset contains normalized spectra of pure milk samples and their mixtures. Different level of adulteration (0.5%, 1%, 5%, 10%, 20%, 40%, 60%, 80%)…
View article: Associations among Farm, Breed, Lactation Stage and Parity, Gene Polymorphisms and the Fatty Acid Profile of Milk from Holstein, Simmental and Their Crosses
Associations among Farm, Breed, Lactation Stage and Parity, Gene Polymorphisms and the Fatty Acid Profile of Milk from Holstein, Simmental and Their Crosses Open
This study aimed to analyze the factors affecting the fatty acid (FA) profile in cow’s milk. The effects of a farm, lactation parity and stage, breed and polymorphisms in the AGPAT6, DGAT1, LEP, FASN and SCD1 genes were evaluated. A total …
View article: Raw Cow Milk Protein Stability under Natural and Technological Conditions of Environment by Analysis of Variance
Raw Cow Milk Protein Stability under Natural and Technological Conditions of Environment by Analysis of Variance Open
Heat stability (HS) is substantial technology property of raw milk. Analysis of sources of HS variation and its regular monitoring can contribute to creating higher added value in the dairy industry. The goal of this analysis was to assess…
View article: Detection of bovine milk adulteration in caprine milk with N-acetyl carbohydrate biomarkers by using 1H nuclear magnetic resonance spectroscopy
Detection of bovine milk adulteration in caprine milk with N-acetyl carbohydrate biomarkers by using 1H nuclear magnetic resonance spectroscopy Open
In a return to tradition, the popularity of caprine milk is on the rise. However, particularly in countries with developed dairy industries based on bovine milk, there is the risk of adulteration with bovine milk, which is a cheaper altern…
View article: Somatic cell score: gene polymorphisms and other effects in Holstein and Simmental cows
Somatic cell score: gene polymorphisms and other effects in Holstein and Simmental cows Open
Objective: The aim of the study was to evaluate the influence of gene polymorphisms and nongenetic factors on the somatic cell score (SCS) in the milk of Holstein (n = 148) and Simmental (n = 73) cows and their crosses (n = 6).Methods: The…
View article: Effect of Increasing Zearalenone Levels on the Technologically Problematic Microorganisms and Food Risky Pathogens (in Vitro)
Effect of Increasing Zearalenone Levels on the Technologically Problematic Microorganisms and Food Risky Pathogens (in Vitro) Open
The aim of this study was to determine the effect of different zearalenone (ZEA) concentrations (0, 10, 100, 250, 500, 1 000 µg/l) on growth of Bacillus cereus, Staphylococcus aureus and Pseudomonas fluorescens in milk. The samples were in…
View article: Iodine content in goat and sheep milk related to herd size, seasonality, farming practice and breed effect
Iodine content in goat and sheep milk related to herd size, seasonality, farming practice and breed effect Open
Iodine is a milk micronutrient whose concentration is highly variable and depends on several factors. The aim of this work was to monitor the prevailing concentration of iodine in raw goat (n = 71) and sheep (n = 68) milk from farms in the…
View article: Association of lipoprotein levels with sleep apnea: role of autonomic dysfunction
Association of lipoprotein levels with sleep apnea: role of autonomic dysfunction Open
Objectives. Although multiple mechanisms, including autonomic dysfunction, seem to link sleep-disordered breathing (SDB) with dyslipidemia in animal studies, the data in clinical studies are limited. The aim of this study was to explore th…
View article: The effect of extended lactation on parameters of Wood’s model of lactation curve in dairy Simmental cows
The effect of extended lactation on parameters of Wood’s model of lactation curve in dairy Simmental cows Open
Objective: This study was focused on the estimation of parameters of Wood’s model and description of the lactation curve using the cows which were lactated over 24 months on the first lactation.Methods: The database included 1,333 pure-bre…
View article: Comparison of Fatty Acid Proportions Determined by Mid-Infrared Spectroscopy and Gas Chromatography in Bulk and Individual Milk Samples
Comparison of Fatty Acid Proportions Determined by Mid-Infrared Spectroscopy and Gas Chromatography in Bulk and Individual Milk Samples Open
Rapid analytical methods can contribute to the expansion of milk fatty acid determination for various important practical purposes. The reliability of data resulting from these routine methods plays a crucial role. Bulk and individual milk…
View article: Technological properties of cow's milk: correlations with milk composition, effect of interactions of genes and other factors
Technological properties of cow's milk: correlations with milk composition, effect of interactions of genes and other factors Open
We analysed the correlations of milk fermentability (yogurt test acidity), renneting (time to rennet coagulation of milk protein) and results of ethanol tests (indirect indicator of milk thermostability) with the percentages of the milk co…
View article: Gene polymorphisms influencing yield, composition and technological properties of milk from Czech Simmental and Holstein cows
Gene polymorphisms influencing yield, composition and technological properties of milk from Czech Simmental and Holstein cows Open
Objective: The aim of the study was to evaluate the influence of polymorphic loci and other factors on milk performance and the technological properties of milk.Methods: The analysis was performed on Simmental and Holstein cows in field co…
View article: Routine Determination of Milk Fat Composition for Nutritional and Technological Purposes
Routine Determination of Milk Fat Composition for Nutritional and Technological Purposes Open
78 individual milk samples (IS) were taken in the Czech Fleckvieh and Holstein dairy cow herds and 33 bulk milk samples (BS) were taken in 13 Holstein dairy cow herds to compare two methods for the determination of fatty acids (FAs) – rout…
View article: Seasonal Correlations Between Heat Stability and Other Raw Bulk Cow Milk Quality Indicators
Seasonal Correlations Between Heat Stability and Other Raw Bulk Cow Milk Quality Indicators Open
Heat milk stability (thermostability, TES) is important technological feature which can contribute to create higher added value in the dairy industry. The aim of this paper was to evaluate the seasonal dynamics and relationships of TES to …
View article: Milk Protein Analysis: an Overview of the Methods - Development and Application
Milk Protein Analysis: an Overview of the Methods - Development and Application Open
Milk protein content is an important component of milk, especially from a nutritional point of view and also for payment purposes. The aim of work was to draw up an overview on reference and routine methods for protein determination. Refer…
View article: Polymorphisms in CSN3, CSN2 and LGB Genes and Their Relation to Milk Production in Dairy Cattle in the Czech Republic
Polymorphisms in CSN3, CSN2 and LGB Genes and Their Relation to Milk Production in Dairy Cattle in the Czech Republic Open
The principal objective of this study was to perform genotyping of the CSN3, CSN2 and LGB genes in selected dairy cattle breeds in the Czech Republic, to determine genotype and allele frequencies and to perform an associative analysis on m…