Explanipedia

Analysis of the role of prespore gene expression in the compartmentalization of mother cell-specific gene expression during sporulation of Bacillus subtilis Open

L Zhang, M L Higgins, P. J. Piggot, Margaret Karow · 1996

A hallmark of sporulation of Bacillus subtilis is the formation of two distinct cells by an asymmetric division. The development programs in these two cells involve the compartmentalized activities of sigma E in the larger mother cell and …

The cytochrome bc complex (menaquinone:cytochrome c reductase) in Bacillus subtilis has a nontraditional subunit organization Open

Jun Yu, Lars Hederstedt, P. J. Piggot · 1995

We have identified an operon in Bacillus subtilis, designated qcr, that is thought to encode a quinone: cytochrome c reductase. Northern (RNA blot) analysis suggests a tricistronic operon. The operon is located at about 200 degrees on the …

Identification of a gene, spoIIR, that links the activation of sigma E to the transcriptional activity of sigma F during sporulation in Bacillus subtilis. Open

Margaret Karow, Philippe Glaser, P. J. Piggot · 1995

Sporulation of Bacillus subtilis requires the coordinated expression of two separate developmental programs in the mother cell and forespore compartments by sigma E and sigma F, respectively. This coordination is maintained through the act…

Analysis by fluorescence microscopy of the development of compartment-specific gene expression during sporulation of Bacillus subtilis Open

Johan Bylund, L Zhang, M A Haines, M L Higgins, P. J. Piggot · 1994

The use of a fluorogenic substrate, 5-octanoylaminofluorescein-di-beta-D-galactopyranoside, for beta-galactosidase has made it possible to visualize enzyme activity in individual cells of sporulating populations of Bacillus subtilis by flu…

Axial filament formation in Bacillus subtilis: induction of nucleoids of increasing length after addition of chloramphenicol to exponential-phase cultures approaching stationary phase Open

Johan Bylund, M A Haines, P. J. Piggot, M L Higgins · 1993

When chloramphenicol was added to a culture of Bacillus subtilis in early exponential growth, microscopic observation of cells stained by 4',6-diamidino-2-phenylindole showed nucleoids that had changed in appearance from irregular spheres …

Characterization of a Bacillus subtilis sporulation operon that includes genes for an RNA polymerase sigma factor and for a putative DD-carboxypeptidase Open

J.-J. Wu, Raymond Schuch, P. J. Piggot · 1992

At early stages of sporulation, the spoIIA locus is transcribed as a tricistronic (1.7-kb) operon, coding for sigma F and for two proteins that modulate the activity of sigma F. The locus is transcribed as a longer (2.9-kb) transcript at t…

Regulation of transcription of the cell division gene ftsA during sporulation of Bacillus subtilis Open

Ahmad Gholamhoseinian, Zhihui Shen, J.-J. Wu, P. J. Piggot · 1992

Three distinct 5' ends of ftsA mRNA were identified by S1 mapping and by primer extension analysis. These are thought to represent three transcription start sites. The transcripts from the downstream and upstream sites were detected throug…

The life‐cycle proteins RodA of Escherichia coli and SpoVE of Bacillus subtilis have very similar primary structures Open

Bernard Joris, Georges Dive, Adriano O. Henriques, P. J. Piggot, Jean‐Marie Ghuysen · 1990

Summary Comparison of the predicted amino acid sequence of the cell‐cycle RodA protein with the National Research Foundation protein sequence database shows that the 370‐amino‐acid RodA, a protein that is essential for wall elongation in E…

Size of the Streptococcus mutans GS-5 chromosome as determined by pulsed-field gel electrophoresis Open

John Tudor, Laura Marri, P. J. Piggot, L Daneo-Moore · 1990

Rare cutting restriction endonucleases were used to cut the Streptococcus mutans chromosome into large fragments. Restriction enzymes utilizing recognition sites containing 6-, 7-, or 8-base-pair sequences with only G and C nucleotides pro…

Timing of spoII gene expression relative to septum formation during sporulation of Bacillus subtilis Open

Ahmad Gholamhoseinian, P. J. Piggot · 1989

spoII mutants formed heat-resistant spores when transformed with spo+ DNa near the start of sporulation. Many of the spores formed remained genetically spoII. It is deduced from this result and previous epistasis experiments that the spoII…

Regulation of transcription of the Bacillus subtilis spoIIA locus Open

J.-J. Wu, M Howard, P. J. Piggot · 1989

The start point of spoIIA transcription was defined by primer extension analysis with two separate primers. It was 27 bases upstream from the putative translation initiation codon of the first open reading frame in the spoIIA locus. A regi…

Complete sequence and transcriptional analysis of the spo0F region of the Bacillus subtilis chromosome Open

K Trach, John Chapman, P. J. Piggot, Dominique Lecoq, J A Hoch · 1988

The total sequence of a 6,314-base-pair BglII fragment of the Bacillus subtilis chromosome containing the spo0F locus has been accomplished. Several genes of interest have been identified on this DNA fragment. The ctrA locus was recognized…

Analysis of the regulation of gene expression during Bacillus subtilis sporulation by manipulation of the copy number of spo-lacZ fusions Open

P. J. Piggot, C.A.M. Curtis · 1987

The control of expression of the Bacillus subtilis spoIIA locus was analyzed by titrating gene expression against gene copy number. A plasmid integrated into the B. subtilis chromosome and carrying the spoIIA control region fused to Escher…

Reduced heat resistance of mutant spores after cloning and mutagenesis of the Bacillus subtilis gene encoding penicillin-binding protein 5 Open

John A. Todd, Anne N. Roberts, K. Johnstone, P. J. Piggot, Greg Winter , et al. · 1986

Part of the gene encoding penicillin-binding protein 5 from Bacillus subtilis 168 was cloned in Escherichia coli with a synthetic oligonucleotide as a hybridization probe. The gene was designated dacA by analogy with E. coli. The nucleotid…

Deduced product of the stage 0 sporulation gene spo0F shares homology with the Spo0A, OmpR, and SfrA proteins. Open

K Trach, John Chapman, P. J. Piggot, J A Hoch · 1985

The location of the stage 0 sporulation locus spo0F has been determined on a cloned fragment of Bacillus subtilis DNA. The spo0F gene and surrounding region was sequenced and was shown to code for a protein of Mr 14,286. The amino acid seq…

Revised genetic linkage map of Bacillus subtilis. Open

P. J. Piggot, J A Hoch · 1985

Characterization of the Replication Terminus of the Bacillus subtilis Chromosome Open

Mervyn J. Monteiro, Michael G. Sargent, P. J. Piggot · 1984

DNA in the terminal region of the chromosome of Bacillus subtilis was labelled by a procedure in which cells in sporulation inducing conditions were pulse-labelled with [3H]thymidine and then treated with p-hydroxyphenylazouracil, an inhib…

Molecular Cloning and Gene Regulation in Bacilli Open

P. J. Piggot · 1984

Genetic aspects of bacterial endospore formation Open

P. J. Piggot, J. G. Coote · 1976

Genetic aspects of bacterial endospore formation. Open

P. J. Piggot, J G Coote · 1976

Statistical Estimate of the Total Number of Operons Specific for Bacillus subtilis Sporulation Open

Daslav Hranueli, P. J. Piggot, J. Mandelstam · 1974

As an alternative to exhaustive mapping, an attempt has been made to obtain a rough estimate of the total number of sporulation operons by statistical treatment. Sixteen sporulation mutants taken at random were characterized biochemically …

Mapping of Asporogenous Mutations of Bacillus subtilis: a Minimum Estimate of the Number of Sporulation Operons Open

P. J. Piggot · 1973

Sporulation-specific mutations in Bacillus subtilis have been mapped by transduction and transformation. The mutations caused blocks at stages 0, II, III, and IV of sporulation; more than one phenotype was found for each of these stages. O…

Ribosomal Alterations Controlling Alkaline Phosphatase Isozymes in Escherichia coli Open

P. J. Piggot, Marshall D. Sklar, Luigi Gorini · 1972

Different patterns of isozymes were obtained by starch-gel electrophoresis of alkaline phosphatase from Escherichia coli strains differing only by strA or ram mutations, or both, in the 30 S ribosomal subunit. The isozyme spread was reduce…

Cyanogen bromide cleavage and partial sequence of the heavy chain of a pathological immunoglobulin G Open

P. J. Piggot, EM Press · 1967

The heavy chain of a pathological immunoglobulin G (Daw) of type L, subclass gamma(2b) (We) and Gm(a+)(f-), has been cleaved with cyanogen bromide. The five fragments resulting from the cleavage have been isolated and analysed. The papain-…

The N- and c-terminal amino acid sequences of the heavy chain from a pathological human immunoglobulin IgG Open

EM Press, P. J. Piggot, RR Porter · 1966

The heavy chain of a pathological human immunoglobulin IgG and also the Fd fragment have been isolated. No free alpha-amino group was present on either and the N-terminal sequence of both has been identified as pyrrolid-2-one-5-carbonylval…

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