Petra J. Kluger
YOU?
Author Swipe
View article: Dairy byproducts as sustainable alternatives to FCS in 2D and 3D skeletal muscle cell cultures
Dairy byproducts as sustainable alternatives to FCS in 2D and 3D skeletal muscle cell cultures Open
View article: Animal‐Free Setup of a 3D Mature Adipocyte‐Macrophage Co‐Culture to Induce Inflammation In Vitro
Animal‐Free Setup of a 3D Mature Adipocyte‐Macrophage Co‐Culture to Induce Inflammation In Vitro Open
Adipose tissue inflammation plays a central role in the pathogenesis of metabolic disorders. It is closely associated with immune cell infiltration, particularly macrophages, and the release of pro‐inflammatory cytokines. Reliable in vitro…
View article: Kultiviertes Fleisch – von Rinderzellen zu biofabriziertem Fett
Kultiviertes Fleisch – von Rinderzellen zu biofabriziertem Fett Open
Cultivated meat could transform the meat market by reducing animal suffering and resource use. As cultured fat is important to achieve the taste and texture of beef, approaches to culture and differentiate primary bovine cells under dynami…
View article: Native and cell-derived extracellular matrix exhibit disparate immunogenic and immunomodulatory effects
Native and cell-derived extracellular matrix exhibit disparate immunogenic and immunomodulatory effects Open
The extracellular matrix (ECM) represents the natural environment of the cells and consists of various fibrous and non-fibrous proteins. It can be generated by decellularization of native tissue (dECM) or by isolation from cultured cells i…
View article: Development of an in vitro three-layered skin wound healing model for pre-clinical testing
Development of an in vitro three-layered skin wound healing model for pre-clinical testing Open
Skin wound healing involves many cell types in a stepwise process of tissue regeneration. Reepithelialization is an essential characteristic of successful healing. In tissue engineering, mimicking the complex process of injury repair in vi…
View article: Completely defined cell culture medium for advanced alveolar models
Completely defined cell culture medium for advanced alveolar models Open
In vitro alveolar models are an important tool study respiratory physiology, investigate lung diseases, develop and test new therapies, and simulate inhalation exposure to environmental pollutants or therapeutic agents. A defined cell cult…
View article: Exploring Morphological and Molecular Properties of Different Adipose Cell Models: Monolayer, Spheroids, Gellan Gum‐Based Hydrogels, and Explants
Exploring Morphological and Molecular Properties of Different Adipose Cell Models: Monolayer, Spheroids, Gellan Gum‐Based Hydrogels, and Explants Open
White adipose tissue (WAT) plays a crucial role in energy homeostasis and secretes numerous adipokines with far‐reaching effects. WAT is linked to diseases such as diabetes, cardiovascular disease, and cancer. There is a high demand for su…
View article: Dynamically cultured, differentiated bovine adipose-derived stem cell spheroids as building blocks for biofabricating cultured fat
Dynamically cultured, differentiated bovine adipose-derived stem cell spheroids as building blocks for biofabricating cultured fat Open
View article: Robot‐based 6D bioprinting for soft tissue biomedical applications
Robot‐based 6D bioprinting for soft tissue biomedical applications Open
Within this interdisciplinary study, we demonstrate the applicability of a 6D printer for soft tissue engineering models. For this purpose, a special plant was constructed, combining the technical requirements for 6D printing with the biol…
View article: Biofabrication's Contribution to the Evolution of Cultured Meat
Biofabrication's Contribution to the Evolution of Cultured Meat Open
Cultured Meat (CM) is a growing field in cellular agriculture, driven by the environmental impact of conventional meat production, which contributes to climate change and occupies ≈70% of arable land. As demand for meat alternatives rises,…
View article: How Mechanical and Physicochemical Material Characteristics Influence Adipose-Derived Stem Cell Fate
How Mechanical and Physicochemical Material Characteristics Influence Adipose-Derived Stem Cell Fate Open
Adipose-derived stem cells (ASCs) are a subpopulation of mesenchymal stem cells. Compared to bone marrow-derived stem cells, they can be harvested with minimal invasiveness. ASCs can be easily expanded and were shown to be able to differen…
View article: Bioprinting of 3D Adipose Tissue Models Using a GelMA-Bioink with Human Mature Adipocytes or Human Adipose-Derived Stem Cells
Bioprinting of 3D Adipose Tissue Models Using a GelMA-Bioink with Human Mature Adipocytes or Human Adipose-Derived Stem Cells Open
Adipose tissue is related to the development and manifestation of multiple diseases, demonstrating the importance of suitable in vitro models for research purposes. In this study, adipose tissue lobuli were explanted, cultured, and used as…
View article: Gellan Gum Is a Suitable Biomaterial for Manual and Bioprinted Setup of Long-Term Stable, Functional 3D-Adipose Tissue Models
Gellan Gum Is a Suitable Biomaterial for Manual and Bioprinted Setup of Long-Term Stable, Functional 3D-Adipose Tissue Models Open
Due to its wide-ranging endocrine functions, adipose tissue influences the whole body’s metabolism. Engineering long-term stable and functional human adipose tissue is still challenging due to the limited availability of suitable biomateri…
View article: Scaffolds for Cultured Meat on the Basis of Polysaccharide Hydrogels Enriched with Plant-Based Proteins
Scaffolds for Cultured Meat on the Basis of Polysaccharide Hydrogels Enriched with Plant-Based Proteins Open
The world population is growing and alternative ways of satisfying the increasing demand for meat are being explored, such as using animal cells for the fabrication of cultured meat. Edible biomaterials are required as supporting structure…
View article: Cell‐derived and enzyme‐based decellularized extracellular matrix exhibit compositional and structural differences that are relevant for its use as a biomaterial
Cell‐derived and enzyme‐based decellularized extracellular matrix exhibit compositional and structural differences that are relevant for its use as a biomaterial Open
Due to its availability and minimal invasive harvesting human adipose tissue‐derived extracellular matrix (dECM) is often used as a biomaterial in various tissue engineering and healthcare applications. Next to dECM, cell‐derived ECM (cdEC…
View article: Homogeneous and Reproducible Mixing of Highly Viscous Biomaterial Inks and Cell Suspensions to Create Bioinks
Homogeneous and Reproducible Mixing of Highly Viscous Biomaterial Inks and Cell Suspensions to Create Bioinks Open
Highly viscous bioinks offer great advantages for the three-dimensional fabrication of cell-laden constructs by microextrusion printing. However, no standardised method of mixing a high viscosity biomaterial ink and a cell suspension has b…
View article: Cell-derived and enzyme-based decellularized extracellular matrix exhibit compositional and structural differences that are relevant for its use as a biomaterial
Cell-derived and enzyme-based decellularized extracellular matrix exhibit compositional and structural differences that are relevant for its use as a biomaterial Open
Due to its availability and minimal invasive harvesting human adipose tissue-derived extracellular matrix (dECM) is often used as a biomaterial in various tissue engineering and healthcare applications. Next to dECM, cell-derived ECM (cdEC…
View article: An Advanced ‘clickECM’ That Can be Modified by the Inverse‐Electron‐Demand Diels‐Alder Reaction
An Advanced ‘clickECM’ That Can be Modified by the Inverse‐Electron‐Demand Diels‐Alder Reaction Open
The extracellular matrix (ECM) represents the natural environment of cells in tissue and therefore is a promising biomaterial in a variety of applications. Depending on the purpose, it is necessary to equip the ECM with specific addressabl…
View article: Cell-derived Extracellular Matrix as maintaining Biomaterial for adipogenic differentiation
Cell-derived Extracellular Matrix as maintaining Biomaterial for adipogenic differentiation Open
The extracellular matrix (ECM) naturally surrounds cells in humans, and therefore represents the ideal biomaterial for tissue engineering. ECM from different tissues exhibit different composition and physical characteristics. Thus, ECM pro…
View article: Adipose stem cell‐derived extracellular matrix represents a promising biomaterial by inducing spontaneous formation of prevascular‐like structures by mvECs
Adipose stem cell‐derived extracellular matrix represents a promising biomaterial by inducing spontaneous formation of prevascular‐like structures by mvECs Open
Tissue constructs of physiologically relevant scale require a vascular system to maintain cell viability. However, in vitro vascularization of engineered tissues is still a major challenge. Successful approaches are based on a feeder layer…
View article: Azido‐functionalized gelatin via direct conversion of lysine amino groups by diazo transfer as a building block for biofunctional hydrogels
Azido‐functionalized gelatin via direct conversion of lysine amino groups by diazo transfer as a building block for biofunctional hydrogels Open
Gelatin is one of the most prominent biopolymers in biomedical material research and development. It is frequently used in hybrid hydrogels, which combine the advantageous properties of bio‐based and synthetic polymers. To prevent the biol…
View article: Improvement of a Three-Layered in vitro Skin Model for Topical Application of Irritating Substances
Improvement of a Three-Layered in vitro Skin Model for Topical Application of Irritating Substances Open
In the field of skin tissue engineering, the development of physiologically relevant in vitro skin models comprising all skin layers, namely epidermis, dermis, and subcutis, is a great challenge. Increasing regulatory requirements a…
View article: Advanced gelatin-based vascularization bioinks for extrusion-based bioprinting of vascularized bone equivalents
Advanced gelatin-based vascularization bioinks for extrusion-based bioprinting of vascularized bone equivalents Open
Bone tissue is highly vascularized. The crosstalk of vascular and osteogenic cells is not only responsible for the formation of the strongly divergent tissue types but also for their physiological maintenance and repair. Extrusion-based bi…
View article: Cell-derived Extracellular Matrix - a promising Biomaterial for in vitro-vascularization in Adipose Tissue Engineering
Cell-derived Extracellular Matrix - a promising Biomaterial for in vitro-vascularization in Adipose Tissue Engineering Open
Tissue constructs of physiologically relevant scale require a vascular system to maintain cell viability. However, in vitro vascularization of engineered tissues is still a major challenge. Successful approaches are based on a feeder layer…
View article: Eclectic characterisation of chemically modified cell-derived matrices obtained by metabolic glycoengineering and re-assessment of commonly used methods
Eclectic characterisation of chemically modified cell-derived matrices obtained by metabolic glycoengineering and re-assessment of commonly used methods Open
Common characterisation methods for cell-derived extracellular matrices (ECMs) are compared using both unmodified and azide-bearing fibroblast-derived ECM.
View article: Generation of an azide-modified extracellular matrix by adipose-derived stem cells using metabolic glycoengineering
Generation of an azide-modified extracellular matrix by adipose-derived stem cells using metabolic glycoengineering Open
Natural extracellular matrix (ECM) represents an ideal biomaterial for tissue engineering and regenerative medicine approaches. For further functionalization, there is a need for specific addressable functional groups within this biomateri…
View article: Author Correction: 3D biodegradable scaffolds of polycaprolactone with silicate-containing hydroxyapatite microparticles for bone tissue engineering: high-resolution tomography and in vitro study
Author Correction: 3D biodegradable scaffolds of polycaprolactone with silicate-containing hydroxyapatite microparticles for bone tissue engineering: high-resolution tomography and in vitro study Open
View article: Biotechnology and Bioengineering: Volume 115, Number 10, October 2018
Biotechnology and Bioengineering: Volume 115, Number 10, October 2018 Open
right asymmetry in tissue microarchitectures.To investigate this phenomenon, fl uorescence staining of nuclei was used to reveal such orientation,
View article: 3D biodegradable scaffolds of polycaprolactone with silicate-containing hydroxyapatite microparticles for bone tissue engineering: high-resolution tomography and in vitro study
3D biodegradable scaffolds of polycaprolactone with silicate-containing hydroxyapatite microparticles for bone tissue engineering: high-resolution tomography and in vitro study Open
To date, special interest has been paid to composite scaffolds based on polymers enriched with hydroxyapatite (HA). However, the role of HA containing different trace elements such as silicate in the structure of a polymer scaffold has not…
View article: Osteoclast Formation within a Human Co-Culture System on Bone Material as an In Vitro Model for Bone Remodeling Processes
Osteoclast Formation within a Human Co-Culture System on Bone Material as an In Vitro Model for Bone Remodeling Processes Open
Bone remodeling can be mimicked in vitro by co-culture models. Based on bone cells, such co-cultures help to study synergistic morphological changes and the impact of materials and applied substances. Hence, we examined the formation of os…