Paul F. Lambert
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View article: Mouse Vocal Fold Permeability In Vivo: Effects of Novel Low‐Tech Injury and Instillation Methods
Mouse Vocal Fold Permeability In Vivo: Effects of Novel Low‐Tech Injury and Instillation Methods Open
Objectives Vocal fold (VF) injury and instillation are emerging techniques to study laryngeal pathologies in mouse models. Most approaches require high‐tech visualization and lengthy anesthesia. Intraperitoneal (IP) naphthalene (NAPH) and …
View article: <i>Mus Musculus</i> papillomavirus MmuPV1 resists restriction by human APOBEC3B
<i>Mus Musculus</i> papillomavirus MmuPV1 resists restriction by human APOBEC3B Open
The single-stranded DNA deaminase APOBEC3B (A3B) is capable of potently restricting the replication of a range of viruses including retroviruses (cDNA) and herpesviruses (genomic DNA). However, these and likely other DNA virus families hav…
View article: E2 displacement of CIP2A from TOPBP1 activates the DNA damage response during papillomavirus life cycles
E2 displacement of CIP2A from TOPBP1 activates the DNA damage response during papillomavirus life cycles Open
The papillomavirus life cycle is intricately linked to epithelial differentiation, and the virus manipulates the differentiation process to facilitate viral production. One such manipulation is activation of the DNA damage response (DDR) w…
View article: Identification and Characterization of MmuPV1 Causing Papillomatosis Outbreak in an Animal Research Facility
Identification and Characterization of MmuPV1 Causing Papillomatosis Outbreak in an Animal Research Facility Open
Mouse papillomavirus (MmuPV1) is the first papillomavirus known to infect laboratory mice, making it an irreplaceable tool for research on papillomaviruses. Despite wide use, standardized techniques for conducting MmuPV1 animal research ar…
View article: Kaposi sarcoma-associated herpesvirus cooperates with Epstein-Barr virus to co-transform a small set of human B cells oncogenically
Kaposi sarcoma-associated herpesvirus cooperates with Epstein-Barr virus to co-transform a small set of human B cells oncogenically Open
KSHV has not been found to transform human B cells alone. We show now it infects both peripheral and tonsillar B cells inefficiently and cooperates with EBV to co-transform a similar, small fraction of cells from both sources. These cells …
View article: Integrated single-cell and spatial analysis identifies context-dependent myeloid-T cell interactions in head and neck cancer immune checkpoint blockade response
Integrated single-cell and spatial analysis identifies context-dependent myeloid-T cell interactions in head and neck cancer immune checkpoint blockade response Open
Background Approximately 15-20% of head and neck cancer squamous cell carcinoma (HNSCC) patients respond favorably to immune checkpoint blockade (ICB). Previous single-cell RNA-Seq (scRNA-Seq) studies identified immune features, including …
View article: MmuPV1 infection of Tmc6/Ever1 or Tmc8/Ever2 deficient FVB mice as a model of βHPV in typical epidermodysplasia verruciformis
MmuPV1 infection of Tmc6/Ever1 or Tmc8/Ever2 deficient FVB mice as a model of βHPV in typical epidermodysplasia verruciformis Open
Typical epidermodysplasia verruciformis (EV) is a rare, autosomal recessive disorder characterized by an unusual susceptibility to infection with specific skin-trophic types of human papillomavirus, principally betapapillomaviruses , and a…
View article: Feasibility of a Community‐Based Aquatic and Peer Support Intervention for People With Musculoskeletal Disorders Delivered via a Cross‐Sector Partnership—A Service Evaluation
Feasibility of a Community‐Based Aquatic and Peer Support Intervention for People With Musculoskeletal Disorders Delivered via a Cross‐Sector Partnership—A Service Evaluation Open
Introduction Worldwide, the unmet rehabilitation needs of adults with musculoskeletal disorders are high. Aquatic exercise is clinically effective in managing musculoskeletal disorders, but access to rehabilitative services is limited. Obj…
View article: An analysis of the virtual cardio-oncology research initiative (VICORI) resource: bleeding risk among cancer patients beyond 1-year post-myocardial infarction
An analysis of the virtual cardio-oncology research initiative (VICORI) resource: bleeding risk among cancer patients beyond 1-year post-myocardial infarction Open
Introduction Cardiovascular disease (CVD) and cancer are common causes of morbidity and mortality. Advancements in treatment strategies for both diseases have resulted in a growing population who live with both conditions. Myocardial infar…
View article: Invasive cancer and spontaneous regression two weeks after papillomavirus infection
Invasive cancer and spontaneous regression two weeks after papillomavirus infection Open
Development of invasive cancer in mammals is thought to require months or years after initial events such as mutation or viral infection. Rarely, invasive cancers regress spontaneously. We show that cancers can develop and regress on a tim…
View article: Increased Susceptibility of WHIM Mice to Papillomavirus-induced Disease is Dependent upon Immune Cell Dysfunction
Increased Susceptibility of WHIM Mice to Papillomavirus-induced Disease is Dependent upon Immune Cell Dysfunction Open
Warts, Hypogammaglobulinemia, Infections, and Myelokathexis (WHIM) syndrome is a rare primary immunodeficiency disease in humans caused by a gain of function in CXCR4, mostly due to inherited heterozygous mutations in CXCR4 . One major cli…
View article: Deficiency in <i>Ever2</i> does not increase susceptibility of mice to pathogenesis by the mouse papillomavirus, MmuPV1
Deficiency in <i>Ever2</i> does not increase susceptibility of mice to pathogenesis by the mouse papillomavirus, MmuPV1 Open
Epidermodysplasia verruciformis (EV) is a rare genetic skin disorder that is characterized by the development of papillomavirus-induced skin lesions that can progress to squamous cell carcinoma (SCC). Certain high-risk, cutaneous β-genus h…
View article: Key aspects of papillomavirus infection influence the host cervicovaginal microbiome in a preclinical murine papillomavirus (MmuPV1) infection model
Key aspects of papillomavirus infection influence the host cervicovaginal microbiome in a preclinical murine papillomavirus (MmuPV1) infection model Open
Human papillomaviruses (HPVs) are the most common sexually transmitted infection in the United States and are a major etiological agent of cancers in the anogenital tract and oral cavity. Growing evidence suggests changes in the host micro…
View article: Non-Metastatic Clear Cell Renal Cell Carcinoma Immune Cell Infiltration Heterogeneity and Prognostic Ability in Patients Following Surgery
Non-Metastatic Clear Cell Renal Cell Carcinoma Immune Cell Infiltration Heterogeneity and Prognostic Ability in Patients Following Surgery Open
Predicting which patients will progress to metastatic disease after surgery for non-metastatic clear cell renal cell carcinoma (ccRCC) is difficult; however, recent data suggest that tumor immune cell infiltration could be used as a biomar…
View article: The Larynx is Protected from Secondary and Vertical Papillomavirus Infection in Immunocompetent Mice
The Larynx is Protected from Secondary and Vertical Papillomavirus Infection in Immunocompetent Mice Open
Objective Mouse papillomavirus MmuPV1 causes both primary and secondary infections of the larynx in immunocompromised mice. Understanding lateral and vertical transmission of papillomavirus to the larynx would benefit patients with recurre…
View article: Emerging Prognostic and Predictive Significance of Stress Keratin 17 in HPV-Associated and Non HPV-Associated Human Cancers: A Scoping Review
Emerging Prognostic and Predictive Significance of Stress Keratin 17 in HPV-Associated and Non HPV-Associated Human Cancers: A Scoping Review Open
A growing body of literature suggests that the expression of cytokeratin 17 (K17) correlates with inferior clinical outcomes across various cancer types. In this scoping review, we aimed to review and map the available clinical evidence of…
View article: Increased Susceptibility of WHIM Mice to Papillomavirus-induced Disease is Dependent upon Immune Cell Dysfunction
Increased Susceptibility of WHIM Mice to Papillomavirus-induced Disease is Dependent upon Immune Cell Dysfunction Open
Warts, Hypogammaglobulinemia, Infections, and Myelokathexis (WHIM) syndrome is a rare primary immunodeficiency disease in humans caused by a gain of function in CXCR4, mostly due to inherited heterozygous mutations in CXCR4 . One major cli…
View article: 215 Stress keratin 17 (K17) expression as a predictor of response to immune check-point blockade (ICB) treated anal, vulvar, and cervical carcinoma
215 Stress keratin 17 (K17) expression as a predictor of response to immune check-point blockade (ICB) treated anal, vulvar, and cervical carcinoma Open
Background The over-expression of stress keratin 17 (K17) has been identified as an immune evasion mechanism in a human papillomavirus (HPV) infection mouse model.1 K17 protein expression has also shown to be a prognostic factor in several…
View article: MmuPV1 E6 induces cell proliferation and other hallmarks of cancer
MmuPV1 E6 induces cell proliferation and other hallmarks of cancer Open
The E6 protein encoded by the murine papillomavirus (MmuPV1) is essential for MmuPV1-induced skin disease. Our previous work has identified a number of cellular interacting partners of MmuPV1 E6 and E7 through affinity purification/mass sp…
View article: 974 Single cell analysis of neutrophil heterogeneity reveals anti-tumoral phenotypes and its implications for immune-checkpoint blockade therapy in head and neck cancer
974 Single cell analysis of neutrophil heterogeneity reveals anti-tumoral phenotypes and its implications for immune-checkpoint blockade therapy in head and neck cancer Open
Background Only ~20% of recurrent and metastatic head and neck squamous cell carcinoma (HNSCC) patients respond to immune-checkpoint blockade (ICB) therapy. A major roadblock is the immunosuppressive nature of the tumor microenvironment. I…
View article: 932 Stress keratin 17 expression in head and neck cancer cells up regulates factors that are involved in M2-macrophage polarization in the tumor microenvironment
932 Stress keratin 17 expression in head and neck cancer cells up regulates factors that are involved in M2-macrophage polarization in the tumor microenvironment Open
Background Stress keratin 17 (K17) is an intermediate filament keratin induced during would healing, inflammatory diseases, papillomavirus infection as well as in cancers. Our prior work described an immune-regulatory role of K17 in three …
View article: Stress Keratin 17 Is a Predictive Biomarker Inversely Associated with Response to Immune Check-Point Blockade in Head and Neck Squamous Cell Carcinomas and Beyond
Stress Keratin 17 Is a Predictive Biomarker Inversely Associated with Response to Immune Check-Point Blockade in Head and Neck Squamous Cell Carcinomas and Beyond Open
Low response rates in immune check-point blockade (ICB)-treated head and neck squamous cell carcinoma (HNSCC) drive a critical need for robust, clinically validated predictive biomarkers. Our group previously showed that stress keratin 17 …
View article: Targeted inhibition of BET proteins in HPV-16 associated head and neck squamous cell carcinoma reveals heterogeneous transcription response
Targeted inhibition of BET proteins in HPV-16 associated head and neck squamous cell carcinoma reveals heterogeneous transcription response Open
Integrated human papillomavirus (HPV-16) associated head and neck squamous cell carcinoma (HNSCC) tumors have worse survival outcomes compared to episomal HPV-16 HNSCC tumors. Therefore, there is a need to differentiate treatment for HPV-1…
View article: Betapapillomaviruses in p16-Negative Vulvar Intraepithelial Lesions Associated with Squamous Cell Carcinoma
Betapapillomaviruses in p16-Negative Vulvar Intraepithelial Lesions Associated with Squamous Cell Carcinoma Open
Approximately 40% of vulvar squamous cell carcinoma (vSCC) cases are etiologically associated with high-risk human papillomaviruses (HPVs) of the alpha genera (α-HPV) that cause other anogenital cancers; however, the etiology of α-HPV-nega…
View article: Hybrid Closed-Loop Therapy in Adults With Type 1 Diabetes and Above-Target HbA1c: A Real-world Observational Study
Hybrid Closed-Loop Therapy in Adults With Type 1 Diabetes and Above-Target HbA1c: A Real-world Observational Study Open
OBJECTIVE We explored longitudinal changes associated with switching to hybrid closed-loop (HCL) insulin delivery systems in adults with type 1 diabetes and elevated HbA1c levels despite the use of intermittently scanned continuous glucose…
View article: Preclinical Models of Laryngeal Papillomavirus Infection: A Scoping Review
Preclinical Models of Laryngeal Papillomavirus Infection: A Scoping Review Open
Objective Laryngeal human papillomavirus (HPV) infection causes recurrent respiratory papillomatosis (RRP) and accounts for up to 25% of laryngeal cancers. Lack of satisfactory preclinical models is one reason that treatments for these dis…