Philippe De Neef
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View article: Ghrelin Is Produced by the Human Erythroleukemic HEL Cell Line and Involved in an Autocrine Pathway Leading to Cell Proliferation
Ghrelin Is Produced by the Human Erythroleukemic HEL Cell Line and Involved in an Autocrine Pathway Leading to Cell Proliferation Open
Ghrelin, a ligand of the GH secretagogue receptor (GHS-R 1a), is a 28-amino acid peptide with an unusual octanoyl group on Ser3, crucial for its biological activity. For the first time, ghrelin and GHS-R 1b, a truncated variant of the rece…
View article: Contribution of the second transmembrane helix of the secretin receptor to the positioning of secretin
Contribution of the second transmembrane helix of the secretin receptor to the positioning of secretin Open
The secretin amino‐terminal residues are essential for high affinity binding to its cognate receptor and for its biological activity. Mutation of the [Asp 3 ] residue of secretin to [Asn 3 ] decreased the ligand's affinity for the rat wild…
View article: Antagonistic properties are shifted back to agonistic properties by further N‐terminal shortening of pituitary adenylate‐cyclase‐activating peptides in human neuroblastoma NB‐OK‐1 cell membranes
Antagonistic properties are shifted back to agonistic properties by further N‐terminal shortening of pituitary adenylate‐cyclase‐activating peptides in human neuroblastoma NB‐OK‐1 cell membranes Open
N‐terminally shortened analogs of the 27‐amino‐acid and 38‐amino‐acid forms of the pituitaryadenylate‐cyclase‐activating neuropeptide, PACAP(1–27) and PACAP(1–38), were synthesized by a solid‐phase method. Systematic deletion of the first …
View article: Structural requirements for the occupancy of pituitary adenylate‐cyclase‐activating‐peptide (PACAP) receptors and adenylate cyclase activation in human neuroblastoma NB‐OK‐1 cell membranes
Structural requirements for the occupancy of pituitary adenylate‐cyclase‐activating‐peptide (PACAP) receptors and adenylate cyclase activation in human neuroblastoma NB‐OK‐1 cell membranes Open
In these structure activity studies, the 46 analogs of the 27‐amino‐acid form of the pituitary‐adenylate‐cyclase‐activating peptide, PACAP(1–27), and the 38‐amino‐acid form, PACAP(1–38), were either monosubstituted or bisubstituted at posi…
View article: The two forms of the pituitary adenylate cyclase activating polypeptide (PACAP (1–27) and PACAP (1–38)) interact with distinct receptors on rat pancreatic AR 4‐2J cell membranes
The two forms of the pituitary adenylate cyclase activating polypeptide (PACAP (1–27) and PACAP (1–38)) interact with distinct receptors on rat pancreatic AR 4‐2J cell membranes Open
The existence of specific receptors for the two PACAPs (Pituirary Adenylate Cyclase Activating Peptides of 27 and 38 amino acids) was previously demonstrated on membranes from the pancreatic acinar cell line AR 4‐2J (Buscail et al., FEBS L…
View article: Characterization and regulation of the expression of scyllatoxin (Leiurotoxin I) receptors in the human neuroblastoma cell line NB‐OK 1
Characterization and regulation of the expression of scyllatoxin (Leiurotoxin I) receptors in the human neuroblastoma cell line NB‐OK 1 Open
125 I‐[Tyr 2 ]scyllatoxin allowed to label a single class of high‐affinity receptors in membranes from the human neuroblastoma cell line NB‐OK 1. The K d of these receptors was 60 pM for scyllatoxin (Leiurotoxin I) and 20 pM for apamin and…
View article: Structural requirements for the binding of the pituitary adenylate‐cyclase‐activating peptide to receptors and adenylate‐cyclase activation in pancreatic and neuronal membranes
Structural requirements for the binding of the pituitary adenylate‐cyclase‐activating peptide to receptors and adenylate‐cyclase activation in pancreatic and neuronal membranes Open
PACAP (pituitary adenylate‐cyclase‐activating peptide)‐binding receptors were investigated in membranes from the rat pancreatic acinar cell line, AR 4‐2J, the rat hippocampus and the human neuroblastoma cell line NB‐OK, by 125 I‐PACAP(1–27…
View article: Presence of highly selective receptors for PACAP (pituitary adenylate cyclase activating peptide) in membranes from the rat pancreatic acinar cell line AR 4‐2J
Presence of highly selective receptors for PACAP (pituitary adenylate cyclase activating peptide) in membranes from the rat pancreatic acinar cell line AR 4‐2J Open
We characterized highly selective receptors for PACAP, the pituitary adenylate cyclase activating peptide, in the tumoral acinar cell line AR 4‐2J derived from the rat pancreas. PACAP, a novel hypothalamic peptide related to vasoactive int…
View article: The phorbol ester TPA stimulates the expression of functional <i>β</i>-adrenoceptors in human T lymphoblasts Molt 3
The phorbol ester TPA stimulates the expression of functional <i>β</i>-adrenoceptors in human T lymphoblasts Molt 3 Open
In crude membranes from human T lymphoblasts Molt 3 cultured under standard conditions, the adenylate cyclase system was stimulated by GTP, its beta gamma-imido analogue (p[NH]ppG,) NaF and forskolin, but not by isoprenaline, prostaglandin…
View article: Properties of vasoactive‐intestinal‐peptide receptors and β‐adrenoceptors in the murine radiation leukemia‐virus‐induced lymphoma cell line BL/VL<sub>3</sub>
Properties of vasoactive‐intestinal‐peptide receptors and β‐adrenoceptors in the murine radiation leukemia‐virus‐induced lymphoma cell line BL/VL<sub>3</sub> Open
Based on radioligand binding and adenylate cyclase activation, functional receptors to vasoactive intestinal peptide(VIP)/helodermin, were shown to coexist with β 2 ‐adrenoceptors and prostaglandin receptors in membranes from a cultured cl…
View article: Molecular characteristics and evidence for internalization of vasoactive‐intestinal‐peptide (VIP) receptors in the tumoral rat‐pancreatic acinar cell line AR 4‐2 J
Molecular characteristics and evidence for internalization of vasoactive‐intestinal‐peptide (VIP) receptors in the tumoral rat‐pancreatic acinar cell line AR 4‐2 J Open
Vasoactive intestinal peptide (VIP) receptors were investigated in the tumoral acinar cell line AR 4‐2 J derived from rat pancreas [ 125 I]Iodo‐VIP binding to cell membranes showed the following IC 50 values for unlabeled peptides: VIP, 0.…
View article: Chemical, immunological and biological properties of peptides like vasoactive‐intestinal‐peptide and peptide‐histidine‐isoleucinamide extracted from the venom of two lizards (<i>Heloderma horridum and Heloderma suspectum</i>)
Chemical, immunological and biological properties of peptides like vasoactive‐intestinal‐peptide and peptide‐histidine‐isoleucinamide extracted from the venom of two lizards (<i>Heloderma horridum and Heloderma suspectum</i>) Open
1. Having previously isolated helodermin, the major peptide like vasoactive‐intestinal‐peptide and peptide‐histidine‐isoleucinamidc, from the venom of the lizard Heloderma suspectum , we decided on a systematic exploration of all (VIP‐PHI)…
View article: Interaction of vasoactive intestinal peptide (VIP) and N‐terminally modified VIP analogs with rat pancreatic, hepatic and pituitary membranes
Interaction of vasoactive intestinal peptide (VIP) and N‐terminally modified VIP analogs with rat pancreatic, hepatic and pituitary membranes Open
Six vascoactive intestinal peptide (VIP) analogs inhibited [ 125 I]iodo‐VIP and [ 125 I]iodo‐helodermin binding to high‐affinity VIP receptors in rat hepatic membrances. They also stimulated adenylate cyclase activity through these recepto…
View article: Propylbenzilylcholine mustard is selective for rat heart muscarinic receptors having a low affinity for agonists
Propylbenzilylcholine mustard is selective for rat heart muscarinic receptors having a low affinity for agonists Open
Propylbenzilylcholine mustard (PrBCM), an irreversible muscarinic antagonist, inactivated receptors with a low affinity for agonists faster than those with a high affinity in rat heart membranes. This result was obtained using either: (a) …
View article: Effects of temperature and ethanol on agonist and antagonist binding to rat heart muscarinic receptors in the absence and presence of GTP
Effects of temperature and ethanol on agonist and antagonist binding to rat heart muscarinic receptors in the absence and presence of GTP Open
The effect of temperature on the binding of four agonists and three antagonists to rat heart muscarinic receptors was studied in the absence and presence of GTP. The binding of agonists to two states (or classes) of receptors, in the absen…
View article: Presence of helodermin‐like peptides of the VIP‐secretin family in mammalian salivary glands and saliva
Presence of helodermin‐like peptides of the VIP‐secretin family in mammalian salivary glands and saliva Open
Helodermin is a biologically active peptide isolated from the venom of the Gila monster lizard ( Heloderma suspectum ) whose structure is related to that of vasoactive intestinal peptide and secretin. Using a specific radioimmunoassay base…
View article: Specific labelling by [<sup>125</sup>I]helodermin of high‐affinity VIP receptors in rat liver membranes
Specific labelling by [<sup>125</sup>I]helodermin of high‐affinity VIP receptors in rat liver membranes Open
Helodermin, a newly isolated peptide from Gila Monster venom, is structurally related to VIP and secretin. When used as radioligand, [ 125 I]helodermin bound rapidly and reversibly to crude rat liver membranes, the dissociation being accel…