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View article: A conserved C-terminal domain of TamB interacts with multiple BamA POTRA domains in Borreliella burgdorferi
A conserved C-terminal domain of TamB interacts with multiple BamA POTRA domains in Borreliella burgdorferi Open
Lyme disease is the leading tick-borne infection in the United States, caused by the pathogenic spirochete Borreliella burgdorferi , formerly known as Borrelia burgdorferi . Diderms, or bacteria with dual-membrane ultrastructure, such as B…
View article: Fructose-1-kinase has pleiotropic roles in<i>Escherichia coli</i>
Fructose-1-kinase has pleiotropic roles in<i>Escherichia coli</i> Open
In Escherichia coli , the master transcription regulator Catabolite Repressor Activator (Cra) regulates >100 genes in central metabolism. Cra binding to DNA is allosterically regulated by binding to fructose-1-phosphate (F-1-P), but the on…
View article: A CURE for the COVID-19 Era: A Vaccine-Focused Online Immunology Laboratory
A CURE for the COVID-19 Era: A Vaccine-Focused Online Immunology Laboratory Open
During the COVID-19 pandemic, universities across the globe quickly shifted to online education. Laboratory courses faced unique challenges and were forced to reevaluate learning objectives and identify creative projects to engage students…
View article: Inter‐species lateral gene transfer focused on the <i>Chlamydia</i> plasticity zone identifies loci associated with immediate cytotoxicity and inclusion stability
Inter‐species lateral gene transfer focused on the <i>Chlamydia</i> plasticity zone identifies loci associated with immediate cytotoxicity and inclusion stability Open
Chlamydia muridarum actively grows in murine mucosae and is a representative model of human chlamydial genital tract disease. In contrast, C. trachomatis infections in mice are limited and rarely cause disease. The factors that contribute …
View article: QAUST: Protein Function Prediction Using Structure Similarity, Protein Interaction, and Functional Motifs
QAUST: Protein Function Prediction Using Structure Similarity, Protein Interaction, and Functional Motifs Open
The number of available protein sequences in public databases is increasing exponentially. However, a significant percentage of these sequences lack functional annotation, which is essential for the understanding of how biological systems …
View article: Sigma 54-Regulated Transcription Is Associated with Membrane Reorganization and Type III Secretion Effectors during Conversion to Infectious Forms of Chlamydia trachomatis
Sigma 54-Regulated Transcription Is Associated with Membrane Reorganization and Type III Secretion Effectors during Conversion to Infectious Forms of Chlamydia trachomatis Open
The factors that control the growth and infectious processes for Chlamydia are still poorly understood. This study used recently developed genetic tools to determine the regulon for one of the key transcription factors encoded by Chlamydia…
View article: Comprehensive genome analysis and comparisons of the swine pathogen, <i>Chlamydia suis</i> reveals unique ORFs and candidate host-specificity factors
Comprehensive genome analysis and comparisons of the swine pathogen, <i>Chlamydia suis</i> reveals unique ORFs and candidate host-specificity factors Open
Chlamydia suis, a ubiquitous swine pathogen, has the potential for zoonotic transmission to humans and often encodes for resistance to the primary treatment antibiotic, tetracycline. Because of this emerging threat, comparative genomics fo…
View article: Structural and ligand binding analyses of the periplasmic sensor domain of RsbU in <i>Chlamydia trachomatis</i> support a role in TCA cycle regulation
Structural and ligand binding analyses of the periplasmic sensor domain of RsbU in <i>Chlamydia trachomatis</i> support a role in TCA cycle regulation Open
Summary Chlamydia trachomatis is an obligate intracellular bacteria that undergo dynamic morphologic and physiologic conversions upon gaining an access to a eukaryotic cell. These conversions likely require the detection of key environment…
View article: Chromosomal Recombination Targets in <i>Chlamydia</i> Interspecies Lateral Gene Transfer
Chromosomal Recombination Targets in <i>Chlamydia</i> Interspecies Lateral Gene Transfer Open
Genome sequence analysis has demonstrated that there is widespread lateral gene transfer among strains within the species C. trachomatis and with other closely related Chlamydia species in laboratory experiments. This is in contrast to the…
View article: Development of Transposon Mutagenesis for Chlamydia muridarum
Development of Transposon Mutagenesis for Chlamydia muridarum Open
Historical issues with genetic manipulation of Chlamydia have prevented rigorous functional genetic characterization of the ∼1,000 genes in chlamydial genomes. Here, we report the development of a transposon mutagenesis system for C. murid…
View article: Transposon Mutagenesis in Chlamydia trachomatis Identifies CT339 as a ComEC Homolog Important for DNA Uptake and Lateral Gene Transfer
Transposon Mutagenesis in Chlamydia trachomatis Identifies CT339 as a ComEC Homolog Important for DNA Uptake and Lateral Gene Transfer Open
Chlamydia trachomatis infections have an immense impact on public health; however, understanding the basic biology and pathogenesis of this organism has been stalled by the limited repertoire of genetic tools. This report describes the suc…
View article: Comparative genomics of swine pathogen Chlamydia suis with human- and mouse-adapted Chlamydia spp
Comparative genomics of swine pathogen Chlamydia suis with human- and mouse-adapted Chlamydia spp Open
Background The phylum, Chlamydiae, contains a unique set of obligately intracellular bacteria with specific host ranges, despite high genomic relatedness between species. Chlamydia suis, a ubiquitous swine pathogen, has the potential for z…
View article: The Loss of Expression of a Single Type 3 Effector (CT622) Strongly Reduces Chlamydia trachomatis Infectivity and Growth
The Loss of Expression of a Single Type 3 Effector (CT622) Strongly Reduces Chlamydia trachomatis Infectivity and Growth Open
Invasion of epithelial cells by the obligate intracellular bacterium Chlamydia trachomatis results in its enclosure inside a membrane-bound compartment termed an inclusion. The bacterium quickly begins manipulating interactions between hos…
View article: Protein-protein interactions with fructose-1-kinase alter function of the central<i>Escherichia coli</i>transcription regulator, Cra
Protein-protein interactions with fructose-1-kinase alter function of the central<i>Escherichia coli</i>transcription regulator, Cra Open
Summary In E. coli , the master transcription regulator Cra regulates >100 genes in central metabolism by binding upstream DNA operator sequences. Genes encoding glycolytic enzymes are repressed, whereas those for gluconeogenesis and the c…
View article: Chlamydia trachomatis-containing vacuole serves as deubiquitination platform to stabilize Mcl-1 and to interfere with host defense
Chlamydia trachomatis-containing vacuole serves as deubiquitination platform to stabilize Mcl-1 and to interfere with host defense Open
Obligate intracellular Chlamydia trachomatis replicate in a membrane-bound vacuole called inclusion, which serves as a signaling interface with the host cell. Here, we show that the chlamydial deubiquitinating enzyme (Cdu) 1 localizes in t…
View article: Author response: Chlamydia trachomatis-containing vacuole serves as deubiquitination platform to stabilize Mcl-1 and to interfere with host defense
Author response: Chlamydia trachomatis-containing vacuole serves as deubiquitination platform to stabilize Mcl-1 and to interfere with host defense Open
Full text Figures and data Side by side Abstract Introduction Results Discussion Materials and methods References Decision letter Author response Article and author information Metrics Abstract Obligate intracellular Chlamydia trachomatis …
View article: Interrogating Genes That Mediate Chlamydia trachomatis Survival in Cell Culture Using Conditional Mutants and Recombination
Interrogating Genes That Mediate Chlamydia trachomatis Survival in Cell Culture Using Conditional Mutants and Recombination Open
Intracellular bacterial pathogens in the family Chlamydiaceae are causes of human blindness, sexually transmitted disease, and pneumonia. Genetic dissection of the mechanisms of chlamydial pathogenicity has been hindered by multiple limita…
View article: Hypothetical protein <scp>CT</scp>398 (<scp>C</scp>ds<scp>Z</scp>) interacts with σ<sup>54</sup> (<scp>R</scp>po<scp>N</scp>)‐holoenzyme and the type III secretion export apparatus in <i>Chlamydia trachomatis</i>
Hypothetical protein <span>CT</span>398 (<span>C</span>ds<span>Z</span>) interacts with σ<sup>54</sup> (<span>R</span>po<span>N</span>)‐holoenzyme and the type III secretion export apparatus in <i>Chlamydia trachomatis</i> Open
A significant challenge to bacteriology is the relatively large proportion of proteins that lack sufficient sequence similarity to support functional annotation (i.e. hypothetical proteins). The aim of this study was to apply protein struc…