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View article: Blood donors as sentinels for genomic surveillance of West Nile virus in Germany using a sensitive amplicon-based sequencing approach
Blood donors as sentinels for genomic surveillance of West Nile virus in Germany using a sensitive amplicon-based sequencing approach Open
This study highlights blood donors as valuable sentinels for WNV genomic surveillance. The validated amplicon-based sequencing approach enables sensitive, scalable genome recovery from low-viremia samples, and when integrated with routine …
View article: Effects of Metformin Hydrochloride on Development and Survival in the Blow Fly <i>Chrysomya megacephala</i> (Fabricius)
Effects of Metformin Hydrochloride on Development and Survival in the Blow Fly <i>Chrysomya megacephala</i> (Fabricius) Open
Chrysomya megacephala (Diptera: Calliphoridae) is a key forensic indicator species used to estimate the minimum postmortem interval (minPMI). However, the presence of pharmaceuticals, such as metformin hydrochloride (an antidiabetic agent)…
View article: Microalgae diversity in Aedes aegypti larvae guts and breeding sites in Nakhon Si Thammarat, Thailand revealed by light microscopy and metabarcoding
Microalgae diversity in Aedes aegypti larvae guts and breeding sites in Nakhon Si Thammarat, Thailand revealed by light microscopy and metabarcoding Open
Aedes aegypti poses a significant threat by transmitting diseases like dengue, chikungunya and Zika, leading to high morbidity and mortality worldwide. In recent years, the role of microalgae in mosquito larvicidal activity has gained atte…
View article: Blood donors as sentinels for genomic surveillance of West Nile virus in Germany (2020–2024) using a sensitive amplicon-based sequencing approach
Blood donors as sentinels for genomic surveillance of West Nile virus in Germany (2020–2024) using a sensitive amplicon-based sequencing approach Open
West Nile virus (WNV) has emerged as a public health concern in Germany since its first detection in 2018, with evidence of expanding geographic spread. Genomic surveillance is critical for tracking viral evolution, identifying introductio…
View article: DNA barcoding of Culicoides biting midges (Diptera: Ceratopogonidae) and detection of Leishmania and other trypanosomatids in southern Thailand
DNA barcoding of Culicoides biting midges (Diptera: Ceratopogonidae) and detection of Leishmania and other trypanosomatids in southern Thailand Open
Background Biting midges of the genus Culicoides play an important role in the transmission of pathogenic arboviruses and parasites. Thailand has documented more than 100 species of Culicoides ; however, several cryptic species complexes r…
View article: Molecular surveillance of bat flies (Diptera: Nycteribiidae and Streblidae) and other ectoparasites in Ratchaburi, Thailand: Unraveling host associations and coronavirus transmission dynamics in the context of zoonotic spillover risk
Molecular surveillance of bat flies (Diptera: Nycteribiidae and Streblidae) and other ectoparasites in Ratchaburi, Thailand: Unraveling host associations and coronavirus transmission dynamics in the context of zoonotic spillover risk Open
This study highlights the importance of molecular tools in characterizing ectoparasites, their blood meal sources, and associated pathogens. It underscores the potential of ectoparasites as non-invasive tools for coronavirus detection in b…
View article: Seroprevalence and preventive practices of dengue and chikungunya among school children in Bangkok: Gaps in prevention and vaccination strategies
Seroprevalence and preventive practices of dengue and chikungunya among school children in Bangkok: Gaps in prevention and vaccination strategies Open
Background Dengue and chikungunya, both transmitted by Aedes mosquitoes, continue to pose significant public health concerns in Thailand, particularly during the rainy season. Despite ongoing vector control efforts, the incidence of infect…
View article: Recent advances in Phlebotomine sand fly research: a review based on studies presented at ISOPS XI
Recent advances in Phlebotomine sand fly research: a review based on studies presented at ISOPS XI Open
The 11th International Symposium on Phlebotomine Sand flies (ISOPS XI) took place in Portorož, Slovenia, in September 2024 and brought together experts from around the world to discuss recent advances in the biology, ecology and control of…
View article: Species distribution and screening of Trypanosoma DNA in phlebotomine sand flies from four southern provinces of Thailand
Species distribution and screening of Trypanosoma DNA in phlebotomine sand flies from four southern provinces of Thailand Open
Sand flies are principal vectors of Leishmania spp. and Trypanosoma spp. Identifying precise vector species is crucial for effective control. We conducted a study on the species distribution of phlebotomine sand flies in cave-dwelling and …
View article: A one-step multiplex qPCR assay for simultaneous identification and quantification of <i>Leishmania martiniquensis</i> and <i>Leishmania orientalis</i>/<i>Leishmania chancei</i> and detection and quantification of trypanosomatids in clinical samples
A one-step multiplex qPCR assay for simultaneous identification and quantification of <i>Leishmania martiniquensis</i> and <i>Leishmania orientalis</i>/<i>Leishmania chancei</i> and detection and quantification of trypanosomatids in clinical samples Open
Leishmaniasis is one of the most important zoonotic diseases. Recently, Leishmania ( Mundinia ) martiniquensis , Leishmania ( Mundinia ) orientalis , and Leishmania ( Mundinia ) chancei have been reported as new human pathogens. Trypanosom…
View article: New insights into molecular characterization and genetic diversity of Eimeria coccidian parasites in bats from diverse geographical regions of Thailand using nanopore-based DNA metabarcoding
New insights into molecular characterization and genetic diversity of Eimeria coccidian parasites in bats from diverse geographical regions of Thailand using nanopore-based DNA metabarcoding Open
Bats represent over 1400 species globally, accounting for approximately one-fifth of all mammalian diversity, yet their gastrointestinal parasite communities remain understudied. Among these parasites, the genus Eimeria is one of the most …
View article: Molecular detection of parasites and host preference in wild-caught <i>Culicoides</i> biting midges (Diptera: Ceratopogonidae) in Chiang Mai and Nakhon Si Thammarat Provinces, Thailand
Molecular detection of parasites and host preference in wild-caught <i>Culicoides</i> biting midges (Diptera: Ceratopogonidae) in Chiang Mai and Nakhon Si Thammarat Provinces, Thailand Open
Culicoides biting midges (Diptera: Ceratopogonidae) have been reported as potential vectors for haemoparasites. Information about host-vector-parasite specificity is required to confirm their status. Here, molecular detection of haemospori…
View article: Seasonal dynamics, Leishmania diversity, and nanopore-based metabarcoding of blood meal origins in Culicoides spp. in the newly emerging focus of leishmaniasis in Northern Thailand
Seasonal dynamics, Leishmania diversity, and nanopore-based metabarcoding of blood meal origins in Culicoides spp. in the newly emerging focus of leishmaniasis in Northern Thailand Open
Background Clinical cases of leishmaniasis caused by Leishmania ( Mundinia ) parasites have been increasingly reported in Southeast Asia, particularly Thailand. Recent evidence has shown that Leishmania ( Mundinia ) parasites successfully …
View article: Seasonal Abundance and Diversity of Culicoides Biting Midges in Livestock Sheds in Kanchanaburi Province, Thailand
Seasonal Abundance and Diversity of Culicoides Biting Midges in Livestock Sheds in Kanchanaburi Province, Thailand Open
Culicoides biting midges were collected using UV light traps from nine livestock farms in Kanchanaburi Province, Thailand. Collections were made one night per month from June 2020 to May 2021 to determine the seasonal changes and spatial d…
View article: Sergentomyia khawi: a potential vector for Leishmania and Trypanosoma parasites affecting humans and animals and insecticide resistance status in endemic areas of Songkhla, southern Thailand
Sergentomyia khawi: a potential vector for Leishmania and Trypanosoma parasites affecting humans and animals and insecticide resistance status in endemic areas of Songkhla, southern Thailand Open
Background Sand flies serve as crucial vectors in various medical and veterinary diseases. Sand fly-borne diseases pose a significant public health burden globally, as the causative agents can infect a diverse range of hosts, leading to se…
View article: Comparative analysis of midgut bacterial communities in Chikungunya virus-infected and non-infected Aedes aegypti Thai laboratory strain mosquitoes
Comparative analysis of midgut bacterial communities in Chikungunya virus-infected and non-infected Aedes aegypti Thai laboratory strain mosquitoes Open
Chikungunya virus (CHIKV) poses a significant global health threat, re-emerging as a mosquito-transmitted pathogen that caused high fever, rash, and severe arthralgia. In Thailand, a notable CHIKV outbreak in 2019–2020 affected approximate…
View article: Nanopore-Based Surveillance of Leishmania Parasites in Culicoides Latrielle (Diptera: Ceratopogonidae) Caught from the Affected Community and Tham Phra Cave in Chiang Rai Province, the Endemic Area of Leishmaniasis in Northern Thailand
Nanopore-Based Surveillance of Leishmania Parasites in Culicoides Latrielle (Diptera: Ceratopogonidae) Caught from the Affected Community and Tham Phra Cave in Chiang Rai Province, the Endemic Area of Leishmaniasis in Northern Thailand Open
In this research, we elucidated the species composition of Culicoides biting midges, infection prevalence, and genetic diversity of Leishmania parasites circulating in the affected community in Chiang Rai Province, being the most endemic a…
View article: Novel duplex TaqMan-based quantitative PCR for rapid and accurate diagnosis of Leishmania (Mundinia) martiniquensis and Leishmania (Mundinia) orientalis, responsible for autochthonous leishmaniasis in Thailand
Novel duplex TaqMan-based quantitative PCR for rapid and accurate diagnosis of Leishmania (Mundinia) martiniquensis and Leishmania (Mundinia) orientalis, responsible for autochthonous leishmaniasis in Thailand Open
The World Health Organization has recently declared Thailand a leishmaniasis hotspot in Southeast Asia due to the continuous increase in new symptomatic and asymptomatic cases over the years. This emerging parasitic disease is known to be …
View article: Persistence of dengue genome in a remotely infected patient
Persistence of dengue genome in a remotely infected patient Open
Background Dengue virus infection is an intriguing illness. It is traditionally thought of as a self-limited and nonpersistent disease. Objectives We report a case with persistent dengue virus genome detectable in hematopoietic cells of a …
View article: Genetic diversity and phylogenetic analyses of Asian lineage of Zika virus whole genome sequencing derived from Culex quinquefasciatus mosquitoes and urine of patients during the epidemic in Thailand
Genetic diversity and phylogenetic analyses of Asian lineage of Zika virus whole genome sequencing derived from Culex quinquefasciatus mosquitoes and urine of patients during the epidemic in Thailand Open
Zika virus (ZIKV) is a mosquito-borne flavivirus that is emerging and re-emerging. Since 2010, sporadic ZIKV cases have been reported annually in Thailand, with over 1,000 confirmed positive cases in 2016. High-throughput sequencing techno…
View article: Phlebotomine sand flies (Diptera, Psychodidae) from Pha Tong cave, Northern Thailand with a description of two new species and taxonomical thoughts about Phlebotomus stantoni
Phlebotomine sand flies (Diptera, Psychodidae) from Pha Tong cave, Northern Thailand with a description of two new species and taxonomical thoughts about Phlebotomus stantoni Open
Background In South-East Asia, Thailand is the country with the highest number of human autochthonous cases of leishmaniases mostly due to Leishmania martiniquensis . Their transmission remains unresolved to date even though sand flies are…
View article: Natural infection with Leishmania (Mundinia) martiniquensis supports Culicoides peregrinus (Diptera: Ceratopogonidae) as a potential vector of leishmaniasis and characterization of a Crithidia sp. isolated from the midges
Natural infection with Leishmania (Mundinia) martiniquensis supports Culicoides peregrinus (Diptera: Ceratopogonidae) as a potential vector of leishmaniasis and characterization of a Crithidia sp. isolated from the midges Open
The prevalence of autochthonous leishmaniasis in Thailand is increasing but the natural vectors that are responsible for transmission remain unknown. Experimental in vivo infections in Culicoides spp. with Leishmania ( Mundinia ) martiniqu…
View article: Enhancement of VGG16 model with multi-view and spatial dropout for classification of mosquito vectors
Enhancement of VGG16 model with multi-view and spatial dropout for classification of mosquito vectors Open
Mosquitoes transmit pathogens that can cause numerous significant infectious diseases in humans and animals such as malaria, dengue fever, chikungunya fever, and encephalitis. Although the VGG16 model is not one of the most advanced CNN ne…
View article: Amphotericin B resistance correlates with increased fitness in vitro and in vivo in Leishmania (Mundinia) martiniquensis
Amphotericin B resistance correlates with increased fitness in vitro and in vivo in Leishmania (Mundinia) martiniquensis Open
Amphotericin B (AmpB) deoxycholate is the available first-line drug used to treat visceral leishmaniasis caused by Leishmania ( Mundinia ) martiniquensis , however, some cases of AmpB treatment failure have been reported in Thailand. Resis…