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View article: PNA-SERS biosensor for label-free detection of SARS-CoV-2 genomic sequences in saliva
PNA-SERS biosensor for label-free detection of SARS-CoV-2 genomic sequences in saliva Open
The COVID-19 pandemic has emphasized the need for rapid, sensitive, and accessible molecular diagnostics. In this study, we present a label-free Surface-Enhanced Raman Spectroscopy (SERS) biosensor for the direct detection of SARS-CoV-2 RN…
View article: A preliminary investigation on hydrogel disks imbibed with enriched transfersomes as promising tool to promote the cutaneous deposition of vaccine antigens
A preliminary investigation on hydrogel disks imbibed with enriched transfersomes as promising tool to promote the cutaneous deposition of vaccine antigens Open
View article: Safety of Zein Nanoparticles on Human Innate Immunity and Inflammation
Safety of Zein Nanoparticles on Human Innate Immunity and Inflammation Open
In recent years, natural polymers have attracted great interest for the development of release systems for vaccine formulations and drug delivery. Zein, a hydrophobic proline-rich protein mixture obtained from maize, is one of the most wid…
View article: PEI‐Engineered Lipid@PLGA Hybrid Nanoparticles for Multimodal Delivery of Antigens and Immune Adjuvants to the Respiratory Mucosa
PEI‐Engineered Lipid@PLGA Hybrid Nanoparticles for Multimodal Delivery of Antigens and Immune Adjuvants to the Respiratory Mucosa Open
Antigen delivery via respiratory mucosal surfaces is an interesting needle‐free option for vaccination. Nonetheless, it demands for the design of especially tailored formulations. Here, lipid/poly(lactic‐co‐glycolic) acid (PLGA) hybrid nan…
View article: Innate and germline immune memory: specificity and heritability of the ancient immune mechanisms for adaptation and survival
Innate and germline immune memory: specificity and heritability of the ancient immune mechanisms for adaptation and survival Open
The immune memory is one of the defensive strategies developed by both unicellular and multicellular organisms for ensuring their integrity and functionality. While the immune memory of the vertebrate adaptive immune system (based on somat…
View article: EFSA Pilot Project on NAMs for the hazard assessment of nanofibers. Lot 2: ‘Exploring the use of gut‐on‐a‐chip models for risk assessments of nanofibers’
EFSA Pilot Project on NAMs for the hazard assessment of nanofibers. Lot 2: ‘Exploring the use of gut‐on‐a‐chip models for risk assessments of nanofibers’ Open
The present project aimed to improve our understanding of potential effect of nanocellulose (NC) ingestion on human health, addressing some key objectives set by the European Food Safety Authority (EFSA). In particular, the use and improve…
View article: Preoperative thalamus volume is not associated with preoperative cognitive impairment (preCI) or postoperative cognitive dysfunction (POCD)
Preoperative thalamus volume is not associated with preoperative cognitive impairment (preCI) or postoperative cognitive dysfunction (POCD) Open
View article: AB0067 MODE OF ACTION AND TARGET INVESTIGATION OF THE NEW ANTI-RHEUMATIC DRUG RABEXIMOD.
AB0067 MODE OF ACTION AND TARGET INVESTIGATION OF THE NEW ANTI-RHEUMATIC DRUG RABEXIMOD. Open
View article: Environmental stress and nanoplastics’ effects on Ciona robusta: regulation of immune/stress-related genes and induction of innate memory in pharynx and gut
Environmental stress and nanoplastics’ effects on Ciona robusta: regulation of immune/stress-related genes and induction of innate memory in pharynx and gut Open
In addition to circulating haemocytes, the immune system of the solitary ascidian Ciona robusta relies on two organs, the pharynx and the gut, and encompasses a wide array of immune and stress-related genes. How the pharynx and the gut of …
View article: Cause or consequence? The role of IL-1 family cytokines and receptors in neuroinflammatory and neurodegenerative diseases
Cause or consequence? The role of IL-1 family cytokines and receptors in neuroinflammatory and neurodegenerative diseases Open
Cytokines and receptors of the IL-1 family are key mediators in innate immune and inflammatory reactions in physiological defensive conditions, but are also significantly involved in immune-mediated inflammatory diseases. Here, we will add…
View article: Heterogeneity of Lipopolysaccharide as Source of Variability in Bioassays and LPS-Binding Proteins as Remedy
Heterogeneity of Lipopolysaccharide as Source of Variability in Bioassays and LPS-Binding Proteins as Remedy Open
Lipopolysaccharide (LPS), also referred to as endotoxin, is the major component of Gram-negative bacteria’s outer cell wall. It is one of the main types of pathogen-associated molecular patterns (PAMPs) that are known to elicit severe immu…
View article: Postoperative delirium is associated with grey matter brain volume loss
Postoperative delirium is associated with grey matter brain volume loss Open
Delirium is associated with long-term cognitive dysfunction and with increased brain atrophy. However, it is unclear whether these problems result from or predisposes to delirium. We aimed to investigate preoperative to postoperative brain…
View article: Induction of Innate Memory in Human Monocytes Exposed to Mixtures of Bacterial Agents and Nanoparticles
Induction of Innate Memory in Human Monocytes Exposed to Mixtures of Bacterial Agents and Nanoparticles Open
We assessed whether concomitant exposure of human monocytes to bacterial agents and different engineered nanoparticles can affect the induction of protective innate memory, an immune mechanism that affords better resistance to diverse thre…
View article: The SARS-CoV-2 Nucleoprotein Induces Innate Memory in Human Monocytes
The SARS-CoV-2 Nucleoprotein Induces Innate Memory in Human Monocytes Open
The interaction of SARS-CoV-2 with the human immune system is at the basis of the positive or negative outcome of the infection. Monocytes and macrophages, which are major innate immune/inflammatory effector cells, are not directly infecte…
View article: Methodological Approaches To Assess Innate Immunity and Innate Memory in Marine Invertebrates and Humans
Methodological Approaches To Assess Innate Immunity and Innate Memory in Marine Invertebrates and Humans Open
Assessing the impact of drugs and contaminants on immune responses requires methodological approaches able to represent real-life conditions and predict long-term effects. Innate immunity/inflammation is the evolutionarily most widespread …
View article: TNFα Mediates Inflammation-Induced Effects on PPARG Splicing in Adipose Tissue and Mesenchymal Precursor Cells
TNFα Mediates Inflammation-Induced Effects on PPARG Splicing in Adipose Tissue and Mesenchymal Precursor Cells Open
Low-grade chronic inflammation and reduced differentiation capacity are hallmarks of hypertrophic adipose tissue (AT) and key contributors of insulin resistance. We identified PPARGΔ5 as a dominant-negative splicing isoform overexpressed i…
View article: In Vitro and In Vivo Models to Assess the Immune-Related Effects of Nanomaterials
In Vitro and In Vivo Models to Assess the Immune-Related Effects of Nanomaterials Open
The immunological safety of drugs, nanomaterials and contaminants is a central point in the regulatory evaluation and safety monitoring of working and public places and of the environment. In fact, anomalies in immune responses may cause d…
View article: The Interactions between Nanoparticles and the Innate Immune System from a Nanotechnologist Perspective
The Interactions between Nanoparticles and the Innate Immune System from a Nanotechnologist Perspective Open
The immune system contributes to maintaining the body’s functional integrity through its two main functions: recognizing and destroying foreign external agents (invading microorganisms) and identifying and eliminating senescent cells and d…
View article: Innate Memory Reprogramming by Gold Nanoparticles Depends on the Microbial Agents That Induce Memory
Innate Memory Reprogramming by Gold Nanoparticles Depends on the Microbial Agents That Induce Memory Open
Innate immune memory, the ability of innate cells to react in a more protective way to secondary challenges, is induced by exposure to infectious and other exogeous and endogenous agents. Engineered nanoparticles are particulate exogenous …
View article: SERS Sensing of Bacterial Endotoxin on Gold Nanoparticles
SERS Sensing of Bacterial Endotoxin on Gold Nanoparticles Open
Engineered gold nanoparticles (AuNPs) find application in several fields related to human activities ( i.e. , food and cosmetic industry or water purification) including medicine, where they are employed for diagnosis, drug delivery and ca…
View article: Personalised Profiling of Innate Immune Memory Induced by Nano-Imaging Particles in Human Monocytes
Personalised Profiling of Innate Immune Memory Induced by Nano-Imaging Particles in Human Monocytes Open
Engineered nanoparticles used for medical purposes must meet stringent safety criteria, which include immunosafety, i.e. , the inability to activate possibly detrimental immune/inflammatory effects. Even medical nanomaterials devoid of dir…
View article: Primary and Memory Response of Human Monocytes to Vaccines: Role of Nanoparticulate Antigens in Inducing Innate Memory
Primary and Memory Response of Human Monocytes to Vaccines: Role of Nanoparticulate Antigens in Inducing Innate Memory Open
Innate immune cells such as monocytes and macrophages are activated in response to microbial and other challenges and mount an inflammatory defensive response. Exposed cells develop the so-called innate memory, which allows them to react d…
View article: Interaction of nanoparticles with endotoxin <i>Importance in nanosafety testing and exploitation for endotoxin binding</i>
Interaction of nanoparticles with endotoxin <i>Importance in nanosafety testing and exploitation for endotoxin binding</i> Open
The interaction between engineered nanoparticles and the bacterial lipopolysaccharide, or endotoxin, is an event that warrants attention. Endotoxin is one of the most potent stimulators of inflammation and immune reactions in human beings,…
View article: Editorial: Immune Mechanisms in the Pathologic Response to Particles, Fibers, and Nanomaterials
Editorial: Immune Mechanisms in the Pathologic Response to Particles, Fibers, and Nanomaterials Open
EDITORIAL article Front. Immunol., 19 March 2021Sec. Molecular Innate Immunity Volume 12 - 2021 | https://doi.org/10.3389/fimmu.2021.665810
View article: Direct LC-MS/MS Analysis of Extra- and Intracellular Glycerophosphoinositol in Model Cancer Cell Lines
Direct LC-MS/MS Analysis of Extra- and Intracellular Glycerophosphoinositol in Model Cancer Cell Lines Open
Glycerophosphoinositols (GPIs) are water-soluble bioactive phospholipid derivatives of increasing interest as intracellular and paracrine mediators of eukaryotic cell functions. The most representative compound of the family is glycerophos…
View article: A Step-by-Step Approach to Improve Clinical Translation of Liposome-Based Nanomaterials, a Focus on Innate Immune and Inflammatory Responses
A Step-by-Step Approach to Improve Clinical Translation of Liposome-Based Nanomaterials, a Focus on Innate Immune and Inflammatory Responses Open
This study aims to provide guidelines to design and perform a robust and reliable physical-chemical characterization of liposome-based nanomaterials, and to support method development with a specific focus on their inflammation-inducing po…
View article: Interaction between Macrophages and Nanoparticles: In Vitro 3D Cultures for the Realistic Assessment of Inflammatory Activation and Modulation of Innate Memory
Interaction between Macrophages and Nanoparticles: In Vitro 3D Cultures for the Realistic Assessment of Inflammatory Activation and Modulation of Innate Memory Open
Understanding the modes of interaction between human monocytes/macrophages and engineered nanoparticles is the basis for assessing particle safety, in terms of activation of innate/inflammatory reactions, and their possible exploitation fo…
View article: The Impact of Nanoparticles on Innate Immune Activation by Live Bacteria
The Impact of Nanoparticles on Innate Immune Activation by Live Bacteria Open
The innate immune system evolved to detect and react against potential dangers such as bacteria, viruses, and environmental particles. The advent of modern technology has exposed innate immune cells, such as monocytes, macrophages, and den…
View article: <em>Gold Nanoparticles contaminated by Bacterial Endotoxin: biophysical characterization, imaging and nanotoxicology</em>
<em>Gold Nanoparticles contaminated by Bacterial Endotoxin: biophysical characterization, imaging and nanotoxicology</em> Open
Gold nanoparticles (AuNPs) are nanodevices that can have many uses in biomedical applications but sometime they show nanotoxic effects on biological system. Among these effects, the activation of the innate immune system (inflammatory resp…
View article: Induction of Innate Immune Memory by Engineered Nanoparticles in Monocytes/Macrophages: From Hypothesis to Reality
Induction of Innate Immune Memory by Engineered Nanoparticles in Monocytes/Macrophages: From Hypothesis to Reality Open
The capacity of engineered nanoparticles to activate cells of the innate immune system, in particular monocytes and macrophages, is considered at the basis of their toxic/inflammatory effects. It is, however, evident that even nanoparticle…