Pascal de Boer
YOU?
Author Swipe
Electron‐Beam Induced Luminescence and Bleaching in Polymer Resins and Embedded Biomaterial Open
Electron microscopy is crucial for imaging biological ultrastructure at nanometer resolution. However, electron irradiation also causes specimen damage, reflected in structural and chemical changes that can give rise to alternative signals…
State‐of‐the‐art microscopy to understand islets of Langerhans: what to expect next? Open
The discovery of Langerhans and microscopic description of islets in the pancreas were crucial steps in the discovery of insulin. Over the past 150 years, many discoveries in islet biology and type 1 diabetes have been made using powerful …
View article: Pancreatic Beta Cell Autophagy is Impaired in Type 1 Diabetes
Pancreatic Beta Cell Autophagy is Impaired in Type 1 Diabetes Open
Aims/hypothesis Pancreatic beta cells are highly metabolic secretory cells that are subjected to exogenous damaging factors such as proinflammatory cytokines or excess glucose that can cause accumulation of damage-inducing reactive oxygen …
TLR3 agonists induce fibronectin aggregation by activated astrocytes: a role of pro-inflammatory cytokines and fibronectin splice variants Open
Multiple sclerosis (MS) is a chronic demyelinating disease of the central nervous system which eventually results in axonal loss mainly due to failure of remyelination. Previously we have shown that the persistent presence of stable astroc…
Use of Negative Bias Potential for High Throughput Array Tomography in an Integrated Light-Electron Microscope Open
Volume electron microscopy (EM) has progressively become a driving force in exploring and analysing three-dimensional biological structures across ever-increasing spatial scales.While advances in technology and automation have revolutioniz…
Integrated Array Tomography for High Throughput Electron Microscopy Open
integrated microscope.Given the scalability of our method, we believe our procedure can pave the way for future large-scale correlative acquisitions-offering a promising approach for the reconstruction of complex biological systems.
Integrated Correlative Light Electron Microscopy for Automated Array Tomography Open
An abstract is not available for this content so a preview has been provided. As you have access to this content, a full PDF is available via the 'Save PDF' action button.
Correlative microscopy reveals abnormalities in type 1 diabetes Open
Microscopische technieken zijn van grote waarde voor onderzoek naar de regulatie van leven en ziekte op het niveau van cellen en moleculen. Ons lab maakt geroutineerd gebruik van een manier om op grote schaal elektronenmicroscopie (EM) dat…
Multi-Color Electron Microscopy by Element-Guided Identification of Cells, Organelles, and Molecules Open
Journal Article Multi-Color Electron Microscopy by Element-Guided Identification of Cells, Organelles, and Molecules Get access M Scotuzzi, M Scotuzzi Imaging Physics, Delft University of Technology, Delft, the Netherlands Search for other…
Multi-color electron microscopy by element-guided identification of cells, organelles and molecules Open
Cellular complexity is unraveled at nanometer resolution using electron microscopy (EM), but interpretation of macromolecular functionality is hampered by the difficulty in interpreting grey-scale images and the unidentified molecular cont…
Nanodiamonds as multi-purpose labels for microscopy Open
Nanodiamonds containing fluorescent nitrogen-vacancy centers are increasingly attracting interest for use as a probe in biological microscopy. This interest stems from (i) strong resistance to photobleaching allowing prolonged fluorescence…
Nanoparticle discrimination based on wavelength and lifetime-multiplexed cathodoluminescence microscopy Open
Nanomaterials can be identified in high-resolution electron microscopy images using lifetime-selective cathodoluminescence.
High‐resolution identification of immuno‐labelling nanoparticles on tissue using <span>X</span> ‐ray detection Open
Cellular structure can be imaged at nanometer resolution using electron microscopy, but the biological molecules remain invisible in grayscale electron micrographs. With Correlative Light and Electron Microscopy (CLEM)[1], fluorescence rec…
Large-scale EM & Correlative Microscopy (Nanotomy & CLEM) Open
A spectrum of dyes and probes now enable to localize molecules of interest within living cells by fluorescence microscopy.With electron microscopy (EM), cellular ultrastructure has been revealed.Bridging these two modalities, correlated li…