Pascal Tomakidi
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View article: Chronic Ethanol Exposure Induces Early Epithelial-to-Mesenchymal Transition (EMT) and Premalignant Changes in Gingival Keratinocytes: An In Vitro Model of Very Early Oral Carcinogenesis
Chronic Ethanol Exposure Induces Early Epithelial-to-Mesenchymal Transition (EMT) and Premalignant Changes in Gingival Keratinocytes: An In Vitro Model of Very Early Oral Carcinogenesis Open
Early molecular events underlying ethanol-induced oral squamous cell carcinoma development remain insufficiently understood, primarily due to a lack of suitable in vitro systems that recapitulate the initial stages of premalignant transfor…
View article: Shaping Orthodontics of the Future: Concepts and Implications from a Cellular and Molecular Perspective
Shaping Orthodontics of the Future: Concepts and Implications from a Cellular and Molecular Perspective Open
Orthodontic tooth movement (OTM) is accompanied by sterile inflammation, a necessary biological process that facilitates tooth displacement but also contributes to adverse effects, including hyalinization and orthodontically induced extern…
View article: Dynamic release kinetics and biological impact of leachables from 3D-printed oral devices: An integrated in vitro and computational exposure model
Dynamic release kinetics and biological impact of leachables from 3D-printed oral devices: An integrated in vitro and computational exposure model Open
Research has highlighted the release of monomers and leachables from additively manufactured (AM) oral devices, raising concerns about their potential biological impact. The oral cavity's dynamic epithelial system necessitates exposure mod…
View article: A Gym for Cells—Direct Laser Writing of Magnetic Multilayered Micro Actuators for Mechanical Stimulation of Cells
A Gym for Cells—Direct Laser Writing of Magnetic Multilayered Micro Actuators for Mechanical Stimulation of Cells Open
Mechanical forces play a crucial role in many biological processes, including cell–cell and cell–matrix interactions. The generation of surface‐attached multilayer micromagnet systems fabricated by two‐photon lithography and the use of suc…
View article: Impairment of Intermediate Filament Expression Reveals Impact on Cell Functions Independent from Keratinocyte Transformation
Impairment of Intermediate Filament Expression Reveals Impact on Cell Functions Independent from Keratinocyte Transformation Open
Although cytoplasmic intermediate filaments (cIFs) are essential for cell physiology, the molecular and cell functional consequences of cIF disturbances are poorly understood. Identifying defaults in cell function-controlled tissue homeost…
View article: Generation of Tailored Multi‐Material Microstructures Through One‐Step Direct Laser Writing
Generation of Tailored Multi‐Material Microstructures Through One‐Step Direct Laser Writing Open
Direct laser writing has gained remarkable popularity by offering architectural control of 3D objects at submicron scales. However, it faces limitations when the fabrication of microstructures comprising multiple materials is desired. The …
View article: Analysis of soft tissue integration‐supportive cell functions in gingival fibroblasts cultured on <scp>3D</scp> printed biomaterials for oral implant‐supported prostheses
Analysis of soft tissue integration‐supportive cell functions in gingival fibroblasts cultured on <span>3D</span> printed biomaterials for oral implant‐supported prostheses Open
To date, it is unknown whether 3D printed fixed oral implant‐supported prostheses can achieve comparable soft tissue integration (STI) to clinically established subtractively manufactured counterparts. STI is mediated among others by gingi…
View article: Two‐Photon Direct Laser Writing of 3D Scaffolds through C, H‐Insertion Crosslinking in a One‐Component Material System
Two‐Photon Direct Laser Writing of 3D Scaffolds through C, H‐Insertion Crosslinking in a One‐Component Material System Open
The popularity of two‐photon direct laser writing in biological research is remarkable as this technique is capable of 3D fabrication of microstructures with unprecedented control, flexibility and precision. Nevertheless, potential impurit…
View article: On the Value of In Vitro Cell Systems for Mechanobiology from the Perspective of Yes-Associated Protein/Transcriptional Co-Activator with a PDZ-Binding Motif and Focal Adhesion Kinase and Their Involvement in Wound Healing, Cancer, Aging, and Senescence
On the Value of In Vitro Cell Systems for Mechanobiology from the Perspective of Yes-Associated Protein/Transcriptional Co-Activator with a PDZ-Binding Motif and Focal Adhesion Kinase and Their Involvement in Wound Healing, Cancer, Aging, and Senescence Open
Mechanobiology comprises how cells perceive different mechanical stimuli and integrate them into a process called mechanotransduction; therefore, the related mechanosignaling cascades are generally important for biomedical research. The on…
View article: An Uncommon Cause of Recurrent Presyncope, Dizziness, and Tachycardia: A Case Report of Diffuse, Adult-Onset Nesidioblastosis/Non-Insulinoma Pancreatogenous Hypoglycemia Syndrome (NIPHS)
An Uncommon Cause of Recurrent Presyncope, Dizziness, and Tachycardia: A Case Report of Diffuse, Adult-Onset Nesidioblastosis/Non-Insulinoma Pancreatogenous Hypoglycemia Syndrome (NIPHS) Open
Neurovegetative and autonomic symptoms are common presentations of various diseases, ranging from psychosomatic to severe organic disorders. A 23-year-old man presented with a history of recurrent presyncope, dizziness, and tachycardia. Re…
View article: Diffuse, Adult-Onset Nesidioblastosis/Non-Insulinoma Pancreatogenous Hypoglycemia Syndrome (NIPHS): Review of the Literature of a Rare Cause of Hyperinsulinemic Hypoglycemia
Diffuse, Adult-Onset Nesidioblastosis/Non-Insulinoma Pancreatogenous Hypoglycemia Syndrome (NIPHS): Review of the Literature of a Rare Cause of Hyperinsulinemic Hypoglycemia Open
Differential diagnosis of hypoglycemia in the non-diabetic adult patient is complex and comprises various diseases, including endogenous hyperinsulinism caused by functional β-cell disorders. The latter is also designated as nesidioblastos…
View article: Characterization of a Stemness-Optimized Purification Method for Human Dental-Pulp Stem Cells: An Approach to Standardization
Characterization of a Stemness-Optimized Purification Method for Human Dental-Pulp Stem Cells: An Approach to Standardization Open
Human dental pulp stem cells (hDPSCs) are promising for oral/craniofacial regeneration, but their purification and characterization is not yet standardized. hDPSCs from three donors were purified by magnetic activated cell sorting (MACS)-a…
View article: Novel In Situ-Cross-Linked Electrospun Gelatin/Hydroxyapatite Nonwoven Scaffolds Prove Suitable for Periodontal Tissue Engineering
Novel In Situ-Cross-Linked Electrospun Gelatin/Hydroxyapatite Nonwoven Scaffolds Prove Suitable for Periodontal Tissue Engineering Open
Periodontal diseases affect millions of people worldwide and can result in tooth loss. Regenerative treatment options for clinical use are thus needed. We aimed at developing new nonwoven-based scaffolds for periodontal tissue engineering.…
View article: Molecular Research on Oral Diseases and Related Biomaterials: A Journey from Oral Cell Models to Advanced Regenerative Perspectives
Molecular Research on Oral Diseases and Related Biomaterials: A Journey from Oral Cell Models to Advanced Regenerative Perspectives Open
Oral diseases such as gingivitis, periodontitis, and oral cancer affect millions of people worldwide. Much research has been conducted to understand the pathogenetic mechanisms of these diseases and translate this knowledge into therapeuti…
View article: FAK Shutdown: Consequences on Epithelial Morphogenesis and Biomarker Expression Involving an Innovative Biomaterial for Tissue Regeneration
FAK Shutdown: Consequences on Epithelial Morphogenesis and Biomarker Expression Involving an Innovative Biomaterial for Tissue Regeneration Open
By employing an innovative biohybrid membrane, the present study aimed at elucidating the mechanistic role of the focal adhesion kinase (FAK) in epithelial morphogenesis in vitro over 4, 7, and 10 days. The consequences of siRNA-mediated F…
View article: From the Matrix to the Nucleus and Back: Mechanobiology in the Light of Health, Pathologies, and Regeneration of Oral Periodontal Tissues
From the Matrix to the Nucleus and Back: Mechanobiology in the Light of Health, Pathologies, and Regeneration of Oral Periodontal Tissues Open
Among oral tissues, the periodontium is permanently subjected to mechanical forces resulting from chewing, mastication, or orthodontic appliances. Molecularly, these movements induce a series of subsequent signaling processes, which are em…
View article: Integrins, cadherins and channels in cartilage mechanotransduction: perspectives for future regeneration strategies
Integrins, cadherins and channels in cartilage mechanotransduction: perspectives for future regeneration strategies Open
Articular cartilage consists of hyaline cartilage, is a major constituent of the human musculoskeletal system and has critical functions in frictionless joint movement and articular homoeostasis. Osteoarthritis (OA) is an inflammatory dise…
View article: Controlling osteoblast morphology and proliferation via surface micro-topographies of implant biomaterials
Controlling osteoblast morphology and proliferation via surface micro-topographies of implant biomaterials Open
Current research on surface modifications has yielded advanced implant biomaterials. Various implant surface modifications have been shown to be promising in improving bone target cell response, but more comprehensive studies whether certa…
View article: PnBA/PDMAA‐Based Iron‐Loaded Micropillars Allow for Discrete Cell Adhesion and Analysis of Actuation‐Related Molecular Responses
PnBA/PDMAA‐Based Iron‐Loaded Micropillars Allow for Discrete Cell Adhesion and Analysis of Actuation‐Related Molecular Responses Open
A new approach to generate magneto‐activatable microstructures for discrete cell adhesion to investigate cell mechanoresponsiveness is reported here. The system is based on poly(n‐butylacrylate) micropillar arrays, generated from prepolyme…
View article: Orthodontic strain affects the Hippo-pathway effector YAP concomitant with proliferation in human periodontal ligament fibroblasts
Orthodontic strain affects the Hippo-pathway effector YAP concomitant with proliferation in human periodontal ligament fibroblasts Open
Our findings provide evidence of ERK1/2 and YAP being biomechanically responsive molecular players in the context of OTM, among which YAP rather than ERK1/2 seems to be mechanistically interrelated with proliferation. Furthermore, the mole…