Patrick Brennwald
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View article: Nickel-NTA lipid-monolayer affinity grids allow for high-resolution structure determination by cryo-EM
Nickel-NTA lipid-monolayer affinity grids allow for high-resolution structure determination by cryo-EM Open
Grid preparation is a rate-limiting step in determining high-resolution structures by single particle cryo-EM. Particle interaction with the air-water interface often leads to denaturation, aggregation, or a preferred orientation within th…
View article: Development and assessment of a sustainable PhD internship program supporting diverse biomedical career outcomes
Development and assessment of a sustainable PhD internship program supporting diverse biomedical career outcomes Open
A doctoral-level internship program was developed at the University of North Carolina at Chapel Hill with the intent to create customizable experiential learning opportunities for biomedical trainees to support career exploration, preparat…
View article: Development and assessment of a sustainable PhD internship program supporting diverse biomedical career outcomes
Development and assessment of a sustainable PhD internship program supporting diverse biomedical career outcomes Open
A doctoral-level internship program was developed at the University of North Carolina at Chapel Hill with the intent to create customizable experiential learning opportunities for biomedical trainees to support career exploration, preparat…
View article: Development and Assessment of a Sustainable PhD Internship Program Supporting Diverse Biomedical Career Outcomes
Development and Assessment of a Sustainable PhD Internship Program Supporting Diverse Biomedical Career Outcomes Open
A doctoral-level internship program was developed at the University of North Carolina at Chapel Hill with the intent to create customizable experiential learning opportunities for biomedical trainees to support career exploration, preparat…
View article: Asymmetric tethering by exocyst in vitro requires a Rab GTPase, an R-SNARE and a Sac1-sensitive phosphoinositide lipid
Asymmetric tethering by exocyst in vitro requires a Rab GTPase, an R-SNARE and a Sac1-sensitive phosphoinositide lipid Open
Tethering factors play a critical role in deciphering the correct combination of vesicle and target membrane, before SNARE complex formation and membrane fusion. The exocyst plays a central role in tethering post-Golgi vesicles to the plas…
View article: Development and assessment of a sustainable PhD internship program supporting diverse biomedical career outcomes
Development and assessment of a sustainable PhD internship program supporting diverse biomedical career outcomes Open
A doctoral-level internship program was developed at the University of North Carolina at Chapel Hill with the intent to create customizable experiential learning opportunities for biomedical trainees to support career exploration, preparat…
View article: Development and Assessment of a Sustainable PhD Internship Program Supporting Diverse Biomedical Career Outcomes
Development and Assessment of a Sustainable PhD Internship Program Supporting Diverse Biomedical Career Outcomes Open
A doctoral-level internship program was developed at the University of North Carolina at Chapel Hill with the intent to create customizable experiential learning opportunities for biomedical trainees to support career exploration, preparat…
View article: Reviewer #1 (Public Review): Development and Assessment of a Sustainable PhD Internship Program Supporting Diverse Biomedical Career Outcomes
Reviewer #1 (Public Review): Development and Assessment of a Sustainable PhD Internship Program Supporting Diverse Biomedical Career Outcomes Open
A doctoral-level internship program was developed at the University of North Carolina at Chapel Hill with the intent to create customizable experiential learning opportunities for biomedical trainees to support career exploration, preparat…
View article: Reviewer #2 (Public Review): Development and Assessment of a Sustainable PhD Internship Program Supporting Diverse Biomedical Career Outcomes
Reviewer #2 (Public Review): Development and Assessment of a Sustainable PhD Internship Program Supporting Diverse Biomedical Career Outcomes Open
A doctoral-level internship program was developed at the University of North Carolina at Chapel Hill with the intent to create customizable experiential learning opportunities for biomedical trainees to support career exploration, preparat…
View article: A cross-institutional analysis of the effects of broadening trainee professional development on research productivity
A cross-institutional analysis of the effects of broadening trainee professional development on research productivity Open
PhD-trained scientists are essential contributors to the workforce in diverse employment sectors that include academia, industry, government, and nonprofit organizations. Hence, best practices for training the future biomedical workforce a…
View article: Asymmetric tethering by exocyst<i>in vitro</i>requires a Rab GTPase, a v-SNARE and a Sac1-sensitive phosphoinositide lipid
Asymmetric tethering by exocyst<i>in vitro</i>requires a Rab GTPase, a v-SNARE and a Sac1-sensitive phosphoinositide lipid Open
Tethering factors play a critical role in deciphering the correct combination of vesicle and target membrane for subsequent fusion. The exocyst plays a central role in tethering post-Golgi vesicles to the plasma membrane, although the mech…
View article: Development and Assessment of a Sustainable PhD Internship Program Supporting Diverse Biomedical Career Outcomes
Development and Assessment of a Sustainable PhD Internship Program Supporting Diverse Biomedical Career Outcomes Open
A doctoral-level internship program was developed at the University of North Carolina at Chapel Hill with the intent to create customizable experiential learning opportunities for biomedical trainees to support career exploration, preparat…
View article: Allosteric regulation of exocyst: Discrete activation of tethering by two spatial signals
Allosteric regulation of exocyst: Discrete activation of tethering by two spatial signals Open
The exocyst imparts spatial control during exocytic vesicle tethering through its interactions with proteins and lipids on the vesicle and the plasma membrane. One such interaction is with the vesicle tether Sro7, although the outcome of t…
View article: The ghost in the machine: small GTPases as spatial regulators of exocytosis
The ghost in the machine: small GTPases as spatial regulators of exocytosis Open
Temporal and spatial regulation of membrane-trafficking events is crucial to both membrane identity and overall cell polarity. Small GTPases of the Rab, Ral and Rho protein families have been implicated as important regulators of vesicle d…
View article: Structurally Conserved Interaction of Lgl Family with SNAREs Is Critical to Their Cellular Function
Structurally Conserved Interaction of Lgl Family with SNAREs Is Critical to Their Cellular Function Open
The Lethal giant larvae (Lgl) tumor suppressor family is conserved from yeast to mammals and plays a critical yet controversial role in cell polarity. Studies on Drosophila Lgl suggest that its function in polarity is through regulation of…
View article: A cross-institutional analysis of the effects of broadening trainee professional development on research productivity
A cross-institutional analysis of the effects of broadening trainee professional development on research productivity Open
PhD-trained scientists are essential contributors to the workforce in diverse employment sectors that include academia, industry, government, and nonprofit organizations. Hence, best practices for training the future biomedical workforce a…
View article: Quantitative Analysis of Membrane Trafficking in Regulation of Cdc42 Polarity
Quantitative Analysis of Membrane Trafficking in Regulation of Cdc42 Polarity Open
Vesicle delivery of Cdc42 has been proposed as an important mechanism for generating and maintaining Cdc42 polarity at the plasma membrane. This mechanism requires the density of Cdc42 on secretory vesicles to be equal to or higher than th…
View article: Quantitative Proteomics of Yeast Post-Golgi Vesicles Reveals a Discriminating Role for Sro7p in Protein Secretion
Quantitative Proteomics of Yeast Post-Golgi Vesicles Reveals a Discriminating Role for Sro7p in Protein Secretion Open
We here report the first comparative proteomics of purified yeast post-Golgi vesicles (PGVs). Vesicle samples isolated from PGV-accumulating sec6-4 mutants were treated with isobaric tags (iTRAQ) for subsequent quantitative tandem mass spe…
View article: Tumor Suppressor Scribble Regulates Assembly of Tight Junctions in the Intestinal Epithelium
Tumor Suppressor Scribble Regulates Assembly of Tight Junctions in the Intestinal Epithelium Open
Formation of the epithelial barrier and apico-basal cell polarity represent two characteristics and mutually dependent features of differentiated epithelial monolayers. They are controlled by special adhesive structures, tight junctions (T…
View article: Yeast Cdc42 functions at a late step in exocytosis, specifically during polarized growth of the emerging bud
Yeast Cdc42 functions at a late step in exocytosis, specifically during polarized growth of the emerging bud Open
The Rho family GTPase Cdc42 is a key regulator of cell polarity and cytoskeletal organization in eukaryotic cells. In yeast, the role of Cdc42 in polarization of cell growth includes polarization of the actin cytoskeleton, which delivers s…
View article: Lethal giant larvae proteins interact with the exocyst complex and are involved in polarized exocytosis
Lethal giant larvae proteins interact with the exocyst complex and are involved in polarized exocytosis Open
The tumor suppressor lethal giant larvae (Lgl) plays a critical role in epithelial cell polarization. However, the molecular mechanism by which Lgl carries out its functions is unclear. In this study, we report that the yeast Lgl proteins …
View article: Rho GTPase regulation of exocytosis in yeast is independent of GTP hydrolysis and polarization of the exocyst complex
Rho GTPase regulation of exocytosis in yeast is independent of GTP hydrolysis and polarization of the exocyst complex Open
Rho GTPases are important regulators of polarity in eukaryotic cells. In yeast they are involved in regulating the docking and fusion of secretory vesicles with the cell surface. Our analysis of a Rho3 mutant that is unable to interact wit…
View article: In Vitro Reconstitution of Rab GTPase-dependent Vesicle Clustering by the Yeast Lethal Giant Larvae/Tomosyn Homolog, Sro7
In Vitro Reconstitution of Rab GTPase-dependent Vesicle Clustering by the Yeast Lethal Giant Larvae/Tomosyn Homolog, Sro7 Open
Intracellular traffic in yeast between the Golgi and the cell surface is mediated by vesicular carriers that tether and fuse in a fashion that depends on the function of the Rab GTPase, Sec4. Overexpression of either of two Sec4 effectors,…
View article: Regulation of Rho GTPase crosstalk, degradation and activity by RhoGDI1
Regulation of Rho GTPase crosstalk, degradation and activity by RhoGDI1 Open
At steady state, most Rho GTPases are bound in the cytosol to Rho Guanine nucleotide Dissociation Inhibitors (RhoGDI) 1. RhoGDIs have generally been considered to passively hold Rho proteins in an inactive state within the cytoplasm. Here …
View article: Spatial regulation of exocytosis and cell polarity: Yeast as a model for animal cells
Spatial regulation of exocytosis and cell polarity: Yeast as a model for animal cells Open
Exocytosis is the major mechanism by which new membrane components are delivered to the cell surface. In most, if not all, eukaryotic cells this is also a highly spatially regulated process that is tightly coordinated with the overall pola…