Patrick Fickers
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View article: Sorbitol Uptake and Oxygen Transfer Shape <scp> <i>AOX1</i> </scp> Promoter Induction in Formate Dehydrogenase‐Deficient <scp> <i>Komagataella phaffii</i> </scp>
Sorbitol Uptake and Oxygen Transfer Shape <span> <i>AOX1</i> </span> Promoter Induction in Formate Dehydrogenase‐Deficient <span> <i>Komagataella phaffii</i> </span> Open
In Komagataella phaffii , the use of formate as an AOX1 promoter (P AOX1 ) inducer in combination with sorbitol, a non‐repressive carbon source, has emerged as a promising alternative to methanol‐based expression systems. Recently, we demo…
View article: Engineering serine metabolism to enhance AOX1 promoter self-induction in a formate dehydrogenase-deficient <i>Komagataella phaffii</i>
Engineering serine metabolism to enhance AOX1 promoter self-induction in a formate dehydrogenase-deficient <i>Komagataella phaffii</i> Open
The methylotrophic yeast Komagataella phaffii is a premier host for recombinant protein (rProt) production, traditionally relying on methanol induction of the alcohol oxidase 1 promoter (pAOX1). However, methanol flammability and associate…
View article: Sorbitol uptake and oxygen transfer shape <i>AOX1</i> promoter induction under methanol-free conditions in <i>Komagataella phaffii</i> lacking formate dehydrogenase
Sorbitol uptake and oxygen transfer shape <i>AOX1</i> promoter induction under methanol-free conditions in <i>Komagataella phaffii</i> lacking formate dehydrogenase Open
For decades, expression systems based on the methanol-regulated AOX1 promoter (p AOX1 ) from the alcohol oxidase 1 gene have served as a benchmark for recombinant protein (rProt) production in Komagataella phaffii . However, methanol-free …
View article: Evaluating the Potential of Gamma‐Glutamylcysteine and Glutathione as Substitutes for <scp>SO<sub>2</sub></scp> in White Wine
Evaluating the Potential of Gamma‐Glutamylcysteine and Glutathione as Substitutes for <span>SO<sub>2</sub></span> in White Wine Open
Sulfur dioxide (SO 2 ) is the most common additive used in winemaking with its antimicrobial and antioxidant properties. However, in recent years, there has been an increasing tendency to reduce the use of excessive SO 2 in wine due to its…
View article: Harnessing yeasts for sustainable succinic acid production: advances in metabolic engineering and biorefinery integration
Harnessing yeasts for sustainable succinic acid production: advances in metabolic engineering and biorefinery integration Open
This review highlights the potential of Yarrowia lipolytica and other yeasts as sustainable producers of bio-based succinic acid (SA), a key platform chemical with applications in bioplastics, solvents, and pharmaceuticals. Recent advances…
View article: Formate from <scp>THF</scp> ‐ <scp>C1</scp> metabolism induces the <i>AOX1</i> promoter in formate dehydrogenase‐deficient <i>Komagataella phaffii</i>
Formate from <span>THF</span> ‐ <span>C1</span> metabolism induces the <i>AOX1</i> promoter in formate dehydrogenase‐deficient <i>Komagataella phaffii</i> Open
In Komagataella phaffii (Pichia pastoris) , formate is a recognized alternative inducer to methanol for expression systems based on the AOX1 promoter (p AOX1 ). By disrupting the formate dehydrogenase encoding FDH1 gene, we converted such …
View article: Improving an Alternative Glycerol Catabolism Pathway in <i>Yarrowia lipolytica</i> to Enhance Erythritol Production
Improving an Alternative Glycerol Catabolism Pathway in <i>Yarrowia lipolytica</i> to Enhance Erythritol Production Open
Engineering the glycerol‐3‐phosphate pathway could enhance erythritol production by accelerating glycerol uptake. However, little work has been conducted on the alternative dihydroxyacetone (DHA) pathway in Yarrowia lipolytica . Herein, th…
View article: A multi reaction kinetic model to describe the enzymatic transesterification reaction of jatropha oil using a fermented solid containing lipases
A multi reaction kinetic model to describe the enzymatic transesterification reaction of jatropha oil using a fermented solid containing lipases Open
The advancement of more precise tools for sustainable process design in enzymatic biodiesel synthesis from renewable sources is crucial. Kinetics of solvent-free transesterification reactions were conducted across a temperature spectrum fr…
View article: Formate from THF-C1 metabolism induces the AOX1 promoter in formate dehydrogenase-deficient<i>Pichia pastoris</i>
Formate from THF-C1 metabolism induces the AOX1 promoter in formate dehydrogenase-deficient<i>Pichia pastoris</i> Open
In Pichia pastoris ( Komagataella phaffii ), formate is a recognized alternative inducer to methanol for expression systems based on the AOX1 promoter (pAOX1). By disrupting the formate dehydrogenase encoding FDH1 gene, we converted such a…
View article: Biosynthesis of the antioxidant γ‐glutamyl‐cysteine with engineered <i>Yarrowia lipolytica</i>
Biosynthesis of the antioxidant γ‐glutamyl‐cysteine with engineered <i>Yarrowia lipolytica</i> Open
The dipeptide γ‐glutamylcysteine (γ‐GC), the first intermediate of glutathione (GSH) synthesis, is considered as a promising drug to reduce or prevent plethora of age‐related disorders such as Alzheimer and Parkinson diseases. The unusual …
View article: Valorisation of Waste Bread for the Production of Yeast Biomass by Yarrowia lipolytica Bioreactor Fermentation
Valorisation of Waste Bread for the Production of Yeast Biomass by Yarrowia lipolytica Bioreactor Fermentation Open
The increase in the wastage of bread, representing 12.5 million tons per year, causes ecological problems, such as the production of methane and CO2, when that waste bread (WB) is improperly managed. To reduce this ecological footprint, a …
View article: Role of Dissimilative Pathway of Komagataella phaffii (Pichia pastoris): Formaldehyde Toxicity and Energy Metabolism
Role of Dissimilative Pathway of Komagataella phaffii (Pichia pastoris): Formaldehyde Toxicity and Energy Metabolism Open
Komagataella phaffii (aka Pichia pastoris) is a yeast able to grow in methanol as the sole carbon and energy source. This substrate is converted into formaldehyde, a toxic intermediary that can either be assimilated to biomass or dissimila…
View article: Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production
Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production Open
Komagataella phaffii (formerly known as Pichia pastoris) has become an increasingly important microorganism for recombinant protein production. This yeast species has gained high interest in an industrial setting for the production of a wi…
View article: Synthesis of Secretory Proteins in Yarrowia lipolytica: Effect of Combined Stress Factors and Metabolic Load
Synthesis of Secretory Proteins in Yarrowia lipolytica: Effect of Combined Stress Factors and Metabolic Load Open
While overproduction of recombinant secretory proteins (rs-Prots) triggers multiple changes in the physiology of the producer cell, exposure to suboptimal growth conditions may further increase that biological response. The environmental c…
View article: What makes <i>Komagataella phaffii</i> non-conventional?
What makes <i>Komagataella phaffii</i> non-conventional? Open
The important industrial protein production host Komagataella phaffii (syn Pichia pastoris) is classified as a non-conventional yeast. But what exactly makes K. phaffii non-conventional? In this review, we set out to address the main diffe…
View article: Recombinant protein production in <i>Pichia pastoris</i>: from transcriptionally redesigned strains to bioprocess optimization and metabolic modelling
Recombinant protein production in <i>Pichia pastoris</i>: from transcriptionally redesigned strains to bioprocess optimization and metabolic modelling Open
Pichia pastoris is one of the most widely used host for the production of recombinant proteins. Expression systems that rely mostly on promoters from genes encoding alcohol oxidase 1 or glyceraldehyde-3-phosphate dehydrogenase have been de…
View article: Metabolic engineering of microorganisms for urban waste valorization
Metabolic engineering of microorganisms for urban waste valorization Open
Waste management is one the major challenges throughout the world. Microbial conversion of urban organic waste into valuable by-products can be used as an approach to advance circular bioeconomy and sustainable development. Recent advances…
View article: Biocompatibility and Cytotoxicity of Gold Nanoparticles: Recent Advances in Methodologies and Regulations
Biocompatibility and Cytotoxicity of Gold Nanoparticles: Recent Advances in Methodologies and Regulations Open
Recent advances in the synthesis of metal nanoparticles (MeNPs), and more specifically gold nanoparticles (AuNPs), have led to tremendous expansion of their potential applications in different fields, ranging from healthcare research to mi…
View article: Bioproducts generation from carboxylate platforms by the non-conventional yeast <i>Yarrowia lipolytica</i>
Bioproducts generation from carboxylate platforms by the non-conventional yeast <i>Yarrowia lipolytica</i> Open
In recent years, there has been a growing interest in the use of renewable sources for bio-based production aiming at developing sustainable and feasible approaches towards a circular economy. Among these renewable sources, organic wastes …
View article: Correction to: Metabolic engineering of Yarrowia lipolytica for thermoresistance and enhanced erythritol productivity
Correction to: Metabolic engineering of Yarrowia lipolytica for thermoresistance and enhanced erythritol productivity Open
An amendment to this paper has been published and can be accessed via the original article.
View article: Metabolic engineering of Yarrowia lipolytica for thermoresistance and enhanced erythritol productivity
Metabolic engineering of Yarrowia lipolytica for thermoresistance and enhanced erythritol productivity Open
Background Functional sugar alcohols have been widely used in the food, medicine, and pharmaceutical industries for their unique properties. Among these, erythritol is a zero calories sweetener produced by the yeast Yarrowia lipolytica . H…
View article: Lipids by Yarrowia lipolytica Strains Cultivated on Glucose in Batch Cultures
Lipids by Yarrowia lipolytica Strains Cultivated on Glucose in Batch Cultures Open
Oleaginous microorganisms, such as Yarrowia lipolytica, accumulate lipids that can have interesting applications in food biotechnology or the synthesis of biodiesel. Y. lipolytica yeast can have many advantages such as wide substrate range…
View article: Engineering Yarrowia lipolytica for the Synthesis of Glutathione from Organic By-Products
Engineering Yarrowia lipolytica for the Synthesis of Glutathione from Organic By-Products Open
Tripeptide glutathione, which plays important roles in many cellular mechanisms, is also a biotechnology-oriented molecule with applications in medicine, food and cosmetic. Here, the engineering of the yeast Yarrowia lipolytica for the pro…
View article: Sugar Alcohols and Organic Acids Synthesis in Yarrowia lipolytica: Where Are We?
Sugar Alcohols and Organic Acids Synthesis in Yarrowia lipolytica: Where Are We? Open
Sugar alcohols and organic acids that derive from the metabolism of certain microorganisms have a panoply of applications in agro-food, chemical and pharmaceutical industries. The main challenge in their production is to reach a productivi…
View article: Applied Microbiology 2016- Pichia process optimization by methanol/sorbitol co-feeding – Patrick Fickers - University of Liege
Applied Microbiology 2016- Pichia process optimization by methanol/sorbitol co-feeding – Patrick Fickers - University of Liege Open
Recombinant protein production driven by AOX1 promoter is challenged by a high oxygen demand and warmth production, especially in large-scale bioreactor. A reliable solution depends on a methanol/sorbitol co-feeding strategy during the ind…