Patrick G. Gallagher
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View article: A novel isoform of tensin-1 promotes actin filament assembly for efficient erythroblast enucleation
A novel isoform of tensin-1 promotes actin filament assembly for efficient erythroblast enucleation Open
Mammalian red blood cells are generated via a terminal erythroid differentiation pathway culminating in cell polarization and enucleation. Actin filament (F-actin) polymerization is critical for enucleation, but the underlying molecular re…
View article: Codanin-1, defective in congenital dyserythropoietic anemia I (CDA-I), regulates erythroid differentiation
Codanin-1, defective in congenital dyserythropoietic anemia I (CDA-I), regulates erythroid differentiation Open
In summary, using cell models recapitulating many features of CDA-I, we have studied and confirmed the importance of Codanin-1 during erythroid differentiation and provide mechanistic insight into how loss of Codanin-1 expression results i…
View article: A mouse model for hemoglobin SC disease recapitulates characteristic human pathologies
A mouse model for hemoglobin SC disease recapitulates characteristic human pathologies Open
Sickle cell disease (SCD) is a common, life-threatening group of disorders caused by missense mutations in the β-globin gene (HBB). Mouse models have helped to elucidate the most common form of SCD (hemoglobin SS [HbSS]; homozygous p.Glu6V…
View article: BMI1 regulates human erythroid self-renewal through both gene repression and gene activation
BMI1 regulates human erythroid self-renewal through both gene repression and gene activation Open
The limited proliferative capacity of erythroid precursors is a major obstacle to generate sufficient in vitro-derived red blood cells for clinical purposes. While BMI1, a Polycomb Repressive Complex 1 member, is both necessary and suffici…
View article: Comprehensive phenotypic and proteomic analyses of human reticulocyte maturation
Comprehensive phenotypic and proteomic analyses of human reticulocyte maturation Open
Reticulocyte maturation, the final stage of erythropoiesis following enucleation, is a dynamic 48- to 72-hour process remodeling multi-lobular, motile, and fragile nascent reticulocytes into deformable, biconcave red blood cells. This matu…
View article: Canonical nuclear envelope protein emerin regulates structure and integrity of the erythrocyte plasma membrane
Canonical nuclear envelope protein emerin regulates structure and integrity of the erythrocyte plasma membrane Open
Red blood cells (RBC) are essential for the survival of aerobic organisms. Mutations in RBC plasma membrane proteins can give rise to a spectrum of diseases characterized by hemolysis, inefficient gas exchange, and anemia. We tested whethe…
View article: A novel isoform of Tensin1 promotes actin filament assembly for efficient erythroblast enucleation
A novel isoform of Tensin1 promotes actin filament assembly for efficient erythroblast enucleation Open
Mammalian red blood cells are generated via a terminal erythroid differentiation pathway culminating in cell polarization and enucleation. Actin filament polymerization is critical for enucleation, but the molecular regulatory mechanisms r…
View article: Hematopoietic Stem Cells Supporting Fetal Erythropoiesis Are Differentially Regulated By Small and Large Ribosomal Subunits
Hematopoietic Stem Cells Supporting Fetal Erythropoiesis Are Differentially Regulated By Small and Large Ribosomal Subunits Open
Diamond Blackfan anemia (DBA) is a congenital bone marrow failure syndrome commonly associated with mutations or deletions of ribosomal genes, leading to protein haploinsufficiency and resulting in nucleolar stress and p53 activation. Clin…
View article: HEXIM1 Regulates Early Erythropoiesis and Participates in Multiple Complexes in Erythroid Cells
HEXIM1 Regulates Early Erythropoiesis and Participates in Multiple Complexes in Erythroid Cells Open
In a healthy adult, steady-state erythropoiesis generates a remarkable ~2.5 million mature RBCs every second. The process of erythropoiesis can be divided into distinct phases: early erythropoiesis- the commitment of multi-lineage progenit…
View article: Dexamethasone Targets E2F4 to Induce Erythroid Progenitor Renewal
Dexamethasone Targets E2F4 to Induce Erythroid Progenitor Renewal Open
Corticosteroids such as dexamethasone (Dex) are the first line of treatment to modulate erythroid output in patients with Diamond Blackfan Anemia (DBA), an inherited bone marrow failure syndrome characterized by ribosomal protein (RP) insu…
View article: A Short Isoform of Tensin1 Is a Novel Regulator of F-Actin Assembly in Human Erythroblasts That Promotes Enucleation
A Short Isoform of Tensin1 Is a Novel Regulator of F-Actin Assembly in Human Erythroblasts That Promotes Enucleation Open
Mammalian red blood cells (RBCs) are generated via a terminal erythroid differentiation pathway culminating in cell polarization and enucleation. Actin filament polymerization is critical for enucleation, with F-actin assembling into a pro…
View article: ALK5/Smad signaling mediates rapamycin therapeutic response and TET2-dependent chromatin remodeling in vascular smooth muscle cell differentiation
ALK5/Smad signaling mediates rapamycin therapeutic response and TET2-dependent chromatin remodeling in vascular smooth muscle cell differentiation Open
Background: Vascular smooth muscle cell (VSMC) phenotypic switching contributes to vascular repair and remodeling but also to pathologies including intimal hyperplasia. The mTORC1 inhibitor rapamycin is an effective drug-eluting stent agen…
View article: Epigenetic Remodeling in Human Coronary Artery Smooth Muscle Cell Phenotypic Switching
Epigenetic Remodeling in Human Coronary Artery Smooth Muscle Cell Phenotypic Switching Open
Background Smooth muscle cell (SMC) dedifferentiation contributes to repair and remodeling, but also cardiovascular pathologies. To understand this plasticity, the epigenetic landscape in SMC phenotypic switching was profiled. Methods Geno…
View article: BMI1 regulates human erythroid self-renewal through both gene repression and gene activation
BMI1 regulates human erythroid self-renewal through both gene repression and gene activation Open
The limited proliferative capacity of erythroid precursors is a major obstacle to generate sufficient numbers of in vitro-derived red blood cells (RBC) for clinical purposes. We and others have determined that BMI1, a member of the polycom…
View article: Joint bleeds in mild hemophilia: Prevalence and clinical characteristics
Joint bleeds in mild hemophilia: Prevalence and clinical characteristics Open
Introduction Joint bleeds are a common and frequent complication associated with hemophilia, increasing the risk of hemophilic arthropathy. It is important to define and characterize the presence of joint complications in mild hemophilia t…
View article: Phenotypic and proteomic characterization of the human erythroid progenitor continuum reveal dynamic changes in cell cycle and in metabolic pathways
Phenotypic and proteomic characterization of the human erythroid progenitor continuum reveal dynamic changes in cell cycle and in metabolic pathways Open
Human erythropoiesis is a complex process leading to the production of 2.5 million red blood cells per second. Following commitment of hematopoietic stem cells to the erythroid lineage, this process can be divided into three distinct stage…
View article: Identification of a Novel Gene Regulatory Element in Human Erythroid Progenitor Cells
Identification of a Novel Gene Regulatory Element in Human Erythroid Progenitor Cells Open
Early erythropoiesis involves commitment of multi-lineage progenitors to erythroid progenitor cells and differentiation into erythroid burst-forming unit (BFU-E) cells, then erythroid colony-forming unit (CFU-E) cells. In contrast to termi…
View article: <i>Rps19 and Rpl5 Play Distinct Roles in hematopoietic Stem Cell maintenance and Erythroid Differentiation</i>
<i>Rps19 and Rpl5 Play Distinct Roles in hematopoietic Stem Cell maintenance and Erythroid Differentiation</i> Open
Mutations in ribosomal proteins (RPs) lead to ribosomopathies. Mechanistic basis underlying the failure of erythropoiesis due to mutations in various RPs is yet to be fully defined. Lack of relevant murine models of ribosomapathies has hin…
View article: HEXIM1 is an essential transcription regulator during human erythropoiesis
HEXIM1 is an essential transcription regulator during human erythropoiesis Open
Regulation of RNA polymerase II (RNAPII) activity is an essential process that governs gene expression; however, its contribution to the fundamental process of erythropoiesis remains unclear. hexamethylene bis-acetamide inducible 1 (HEXIM1…
View article: A Novel β-Globin Locus Deletional Syndrome: εγ-Thalassemia
A Novel β-Globin Locus Deletional Syndrome: εγ-Thalassemia Open
Journal Article A Novel β-Globin Locus Deletional Syndrome: εγ-Thalassemia Get access Patrick G Gallagher Patrick G Gallagher Department of Pediatrics, Center for Perinatal Research, Abigail Wexner Research Institute, Nationwide Children's…
View article: Gender Analysis of Journal of Perinatology Authorship During COVID-19
Gender Analysis of Journal of Perinatology Authorship During COVID-19 Open
Objective To examine authorship gender distributions before and during COVID-19 in the Journal of Perinatology. Study Design We collected data from the Journal of Perinatology website. Author gender was determined using Genderize.io or a s…
View article: Phase 3, multicentre, randomised, placebo-controlled study evaluating the efficacy and safety of ustekinumab in patients with systemic lupus erythematosus
Phase 3, multicentre, randomised, placebo-controlled study evaluating the efficacy and safety of ustekinumab in patients with systemic lupus erythematosus Open
NCT03517722.
View article: Anemia in the pediatric patient
Anemia in the pediatric patient Open
The World Health Organization estimates that approximately a quarter of the world’s population suffers from anemia, including almost half of preschool-age children. Globally, iron deficiency anemia is the most common cause of anemia. Other…