Patrick J. Heizer
YOU?
Author Swipe
View article: Differential regulation of Treg stability in human naïve and effector Treg subsets by TGFβ-signaling via ARKADIA-SKI axis
Differential regulation of Treg stability in human naïve and effector Treg subsets by TGFβ-signaling via ARKADIA-SKI axis Open
Introduction The human FOXP3 + regulatory T (Treg) cells, a subset of CD4 + T cells with immunosuppressive function, are essential for the maintenance of immune homeostasis and tolerance. Treg cells are a heterogeneous population, subdivid…
View article: The accumulation of progerin underlies the loss of aortic smooth muscle cells in Hutchinson-Gilford progeria syndrome
The accumulation of progerin underlies the loss of aortic smooth muscle cells in Hutchinson-Gilford progeria syndrome Open
Hutchinson-Gilford progeria syndrome (HGPS) is caused by progerin, an internally truncated prelamin A that does not undergo the ZMPSTE24 processing step that releases prelamin A’s farnesylated carboxyl terminus; consequently, progerin rema…
View article: AAV-mediated co-expression of an immunogenic transgene plus PD-L1 enables sustained expression through immunological evasion
AAV-mediated co-expression of an immunogenic transgene plus PD-L1 enables sustained expression through immunological evasion Open
Adeno-associated virus (AAV) vectors can mediate long-term expression of immunogenic transgenes in vivo through transduction of tolerogenic cells in the liver. Tissue-targeted AAV vectors allow transduction of non-hepatic cells, but this n…
View article: The Accumulation of Progerin Underlies the Loss of Aortic Smooth Muscle Cells in Hutchinson-Gilford Progeria Syndrome
The Accumulation of Progerin Underlies the Loss of Aortic Smooth Muscle Cells in Hutchinson-Gilford Progeria Syndrome Open
Hutchinson-Gilford progeria syndrome (HGPS) is a progeroid disorder characterized by multiple aging-like phenotypes, including disease in large arteries. HGPS is caused by an internally truncated prelamin A (progerin) that cannot undergo t…
View article: Intracapillary LPL levels in brown adipose tissue, visualized with an antibody-based approach, are regulated by ANGPTL4 at thermoneutral temperatures
Intracapillary LPL levels in brown adipose tissue, visualized with an antibody-based approach, are regulated by ANGPTL4 at thermoneutral temperatures Open
Lipoprotein lipase (LPL) is secreted into the interstitial spaces by parenchymal cells and then transported into capillaries by GPIHBP1. LPL carries out the lipolytic processing of triglyceride (TG)-rich lipoproteins (TRLs), but the tissue…
View article: Nuclear membrane ruptures underlie the vascular pathology in a mouse model of Hutchinson-Gilford progeria syndrome
Nuclear membrane ruptures underlie the vascular pathology in a mouse model of Hutchinson-Gilford progeria syndrome Open
The mutant nuclear lamin protein (progerin) produced in Hutchinson-Gilford progeria syndrome (HGPS) results in loss of arterial smooth muscle cells (SMCs), but the mechanism has been unclear. We found that progerin induces repetitive nucle…
View article: Increased expression of LAP2β eliminates nuclear membrane ruptures in nuclear lamin–deficient neurons and fibroblasts
Increased expression of LAP2β eliminates nuclear membrane ruptures in nuclear lamin–deficient neurons and fibroblasts Open
Significance Deficiencies or defects in nuclear lamins result in cell toxicity and cause multiple human diseases. Nuclear membrane (NM) ruptures have been implicated in both cell toxicity and disease pathogenesis, but the factors that modu…
View article: Cultured macrophages transfer surplus cholesterol into adjacent cells in the absence of serum or high-density lipoproteins
Cultured macrophages transfer surplus cholesterol into adjacent cells in the absence of serum or high-density lipoproteins Open
Significance By ingesting apoptotic cells and other cellular debris, macrophages accumulate cholesterol. Some of the cholesterol is esterified and stored in cytosolic lipid droplets, mitigating the toxicity from free cholesterol. Eventuall…
View article: Deficiency in ZMPSTE24 and resulting farnesyl–prelamin A accumulation only modestly affect mouse adipose tissue stores
Deficiency in ZMPSTE24 and resulting farnesyl–prelamin A accumulation only modestly affect mouse adipose tissue stores Open
View article: An absence of lamin B1 in migrating neurons causes nuclear membrane ruptures and cell death
An absence of lamin B1 in migrating neurons causes nuclear membrane ruptures and cell death Open
Significance Deficiencies in lamin B1 or lamin B2 in mice result in markedly reduced neuronal density in the cerebral cortex, but the mechanism has been unclear. We found that deficiencies of either lamin B1 or lamin B2 cause nuclear membr…
View article: Release of cholesterol-rich particles from the macrophage plasma membrane during movement of filopodia and lamellipodia
Release of cholesterol-rich particles from the macrophage plasma membrane during movement of filopodia and lamellipodia Open
Cultured mouse peritoneal macrophages release large numbers of ~30-nm cholesterol-rich particles. Here, we show that those particles represent fragments of the plasma membrane that are pulled away and left behind during the projection and …
View article: Author response: Release of cholesterol-rich particles from the macrophage plasma membrane during movement of filopodia and lamellipodia
Author response: Release of cholesterol-rich particles from the macrophage plasma membrane during movement of filopodia and lamellipodia Open
View article: GPIHBP1 expression in gliomas promotes utilization of lipoprotein-derived nutrients
GPIHBP1 expression in gliomas promotes utilization of lipoprotein-derived nutrients Open
GPIHBP1, a GPI-anchored protein of capillary endothelial cells, binds lipoprotein lipase (LPL) within the subendothelial spaces and shuttles it to the capillary lumen. GPIHBP1-bound LPL is essential for the margination of triglyceride-rich…
View article: Author response: GPIHBP1 expression in gliomas promotes utilization of lipoprotein-derived nutrients
Author response: GPIHBP1 expression in gliomas promotes utilization of lipoprotein-derived nutrients Open
Article Figures and data Abstract Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract GPIHBP1, a GPI-anchored protein of capilla…
View article: Lipoprotein lipase is active as a monomer
Lipoprotein lipase is active as a monomer Open
Significance Lipoprotein lipase (LPL) plays a central role in plasma lipid metabolism, hydrolyzing the triglycerides in lipoproteins and releasing fatty acid nutrients for vital tissues. LPL is synthesized by adipocytes and myocytes and se…
View article: An upstream enhancer regulates Gpihbp1 expression in a tissue-specific manner
An upstream enhancer regulates Gpihbp1 expression in a tissue-specific manner Open
Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 (GPIHBP1), the protein that shuttles LPL to the capillary lumen, is essential for plasma triglyceride metabolism. When GPIHBP1 is absent, LPL remains stranded…
View article: Disrupting the LINC complex in smooth muscle cells reduces aortic disease in a mouse model of Hutchinson-Gilford progeria syndrome
Disrupting the LINC complex in smooth muscle cells reduces aortic disease in a mouse model of Hutchinson-Gilford progeria syndrome Open
Disrupting the LINC complex reduces aortic disease in a mouse model of Hutchinson-Gilford progeria syndrome.
View article: NanoSIMS imaging reveals unexpected heterogeneity in nutrient uptake by brown adipocytes
NanoSIMS imaging reveals unexpected heterogeneity in nutrient uptake by brown adipocytes Open
View article: Macrophages release plasma membrane-derived particles rich in accessible cholesterol
Macrophages release plasma membrane-derived particles rich in accessible cholesterol Open
Significance Earlier studies suggested that particles are released from the macrophage plasma membrane, but the mechanism has been unclear. We found that filopodia of macrophages release large numbers of vesicular particles. Nanoscale seco…
View article: NanoSIMS Analysis of Intravascular Lipolysis and Lipid Movement across Capillaries and into Cardiomyocytes
NanoSIMS Analysis of Intravascular Lipolysis and Lipid Movement across Capillaries and into Cardiomyocytes Open
View article: Impaired thermogenesis and sharp increases in plasma triglyceride levels in GPIHBP1-deficient mice during cold exposure
Impaired thermogenesis and sharp increases in plasma triglyceride levels in GPIHBP1-deficient mice during cold exposure Open
View article: Palmoplantar keratoderma in Slurp1/Slurp2 double-knockout mice
Palmoplantar keratoderma in Slurp1/Slurp2 double-knockout mice Open
View article: Apolipoprotein C-III inhibits triglyceride hydrolysis by GPIHBP1-bound LPL
Apolipoprotein C-III inhibits triglyceride hydrolysis by GPIHBP1-bound LPL Open
View article: Mutating a conserved cysteine in GPIHBP1 reduces amounts of GPIHBP1 in capillaries and abolishes LPL binding
Mutating a conserved cysteine in GPIHBP1 reduces amounts of GPIHBP1 in capillaries and abolishes LPL binding Open
View article: A hypomorphic <i>Egfr</i> allele does not ameliorate the palmoplantar keratoderma caused by SLURP1 deficiency
A hypomorphic <i>Egfr</i> allele does not ameliorate the palmoplantar keratoderma caused by SLURP1 deficiency Open
Mutations in SLURP1 , a secreted protein of keratinocytes, cause a palmoplantar keratoderma (PPK) known as mal de Meleda . Slurp1 deficiency in mice faithfully recapitulates the human disease, with increased keratinocyte proliferation and …