Patrick J. Rochette
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View article: Harnessing Diversity Generating Retroelements for <i>in vivo</i> targeted hyper-mutagenesis
Harnessing Diversity Generating Retroelements for <i>in vivo</i> targeted hyper-mutagenesis Open
The rapid evolution of novel functions requires targeted mutagenesis to avoid harmful mutations. Diversity-generating retroelements (DGRs) are natural systems that accelerate the evolution of diverse bacterial functions through targeted hy…
View article: Localization of fluorescent gold nanoparticles throughout the eye after topical administration
Localization of fluorescent gold nanoparticles throughout the eye after topical administration Open
The human eye is a highly intricate sensory organ. When a condition requiring treatment occurs, eyedrops, which represent 90% of all ophthalmic treatments, are most frequently used. However, eyedrops are associated with low bioavailability…
View article: Synergistic Toxicity of Pollutant and Ultraviolet Exposure from a Mitochondrial Perspective
Synergistic Toxicity of Pollutant and Ultraviolet Exposure from a Mitochondrial Perspective Open
Ultraviolet (UV) exposure and atmospheric pollution are both independently implicated in skin diseases such as cancer and premature aging. UVA wavelengths, which penetrate in the deep layers of the skin dermis, exert their toxicity mainly …
View article: HSDL2 links nutritional cues to bile acid and cholesterol homeostasis
HSDL2 links nutritional cues to bile acid and cholesterol homeostasis Open
In response to energy and nutrient shortage, the liver triggers several catabolic processes to promote survival. Despite recent progress, the precise molecular mechanisms regulating the hepatic adaptation to fasting remain incompletely cha…
View article: Expression and Impact of Fibronectin, Tenascin-C, Osteopontin, and Type XIV Collagen in Fuchs Endothelial Corneal Dystrophy
Expression and Impact of Fibronectin, Tenascin-C, Osteopontin, and Type XIV Collagen in Fuchs Endothelial Corneal Dystrophy Open
This study highlights new abnormal ECM components in FECD, suggests a certain chronology in their deposition, and demonstrates their impact on cell behavior.
View article: Lipid Peroxidation as the Mechanism Underlying Polycyclic Aromatic Hydrocarbons and Sunlight Synergistic Toxicity in Dermal Fibroblasts
Lipid Peroxidation as the Mechanism Underlying Polycyclic Aromatic Hydrocarbons and Sunlight Synergistic Toxicity in Dermal Fibroblasts Open
Light and atmospheric pollution are both independently implicated in cancer induction and premature aging. Evidence has been growing more recently on the toxic synergy between light and pollutants. Polycyclic aromatic hydrocarbons (PAHs) o…
View article: Indenopyrene and Blue-Light Co-Exposure Impairs the Tightly Controlled Activation of Xenobiotic Metabolism in Retinal Pigment Epithelial Cells: A Mechanism for Synergistic Toxicity
Indenopyrene and Blue-Light Co-Exposure Impairs the Tightly Controlled Activation of Xenobiotic Metabolism in Retinal Pigment Epithelial Cells: A Mechanism for Synergistic Toxicity Open
High energy visible (HEV) blue light is an increasing source of concern for visual health. Polycyclic aromatic hydrocarbons (PAH), a group of compounds found in high concentrations in smokers and polluted environments, accumulate in the re…
View article: Assessing the safety of new germicidal<scp>far‐UVC</scp>technologies
Assessing the safety of new germicidal<span>far‐UVC</span>technologies Open
The COVID‐19 pandemic underscored the crucial importance of enhanced indoor air quality control measures to mitigate the spread of respiratory pathogens. Far‐UVC is a type of germicidal ultraviolet technology, with wavelengths between 200 …
View article: The combination of cigarette smoke and solar rays causes effects similar to skin aging in a bilayer skin model
The combination of cigarette smoke and solar rays causes effects similar to skin aging in a bilayer skin model Open
Skin aging is a multifactorial process influenced by internal and external factors. The contribution of different environmental factors has been well established individually in the last few years. On the one hand, man is rarely exposed to…
View article: Integrated Indigenous Management of Land and Marine Protected Areas in Teahupo`o (Tahiti, French Polynesia): A way to enhance ecological and cultural resilience
Integrated Indigenous Management of Land and Marine Protected Areas in Teahupo`o (Tahiti, French Polynesia): A way to enhance ecological and cultural resilience Open
International audience
View article: The Presence of Guttae in Fuchs Endothelial Corneal Dystrophy Explants Correlates With Cellular Markers of Disease Progression
The Presence of Guttae in Fuchs Endothelial Corneal Dystrophy Explants Correlates With Cellular Markers of Disease Progression Open
PURPOSE: Fuchs endothelial corneal dystrophy (FECD) is characterized by an accelerated depletion of corneal endothelial cells. There is growing evidence that mitochondrial exhaustion is central in the pathology. Indeed, endothelial cells l…
View article: Reduction of DNA damage repair efficiency and accumulation of residual damage following chronic UVB-irradiation of HaCaT cells
Reduction of DNA damage repair efficiency and accumulation of residual damage following chronic UVB-irradiation of HaCaT cells Open
Absorption of ultraviolet radiation (UVR) by DNA leads to the predominant formation of cyclobutane pyrimidine dimers (CPD). Since those CPD are responsible for the driver mutations found in skin cancers, their efficient repair is critical.…
View article: Rescuing cellular function in Fuchs endothelial corneal dystrophy by healthy exogenous mitochondrial internalization
Rescuing cellular function in Fuchs endothelial corneal dystrophy by healthy exogenous mitochondrial internalization Open
Fuchs endothelial corneal dystrophy (FECD) is characterized by an accelerated loss of corneal endothelial cells. Since the function of these cells is to maintain the cornea in a state of deturgescence necessary for its transparency, the de…
View article: The Influence of Blue-Filtering Intraocular Lenses Implant on Exudative Age-Related Macular Degeneration: A Case–Control Study
The Influence of Blue-Filtering Intraocular Lenses Implant on Exudative Age-Related Macular Degeneration: A Case–Control Study Open
No correlations could be established between the presence of a blue light filter in the IOL and the occurrence of wet AMD. AMD patients without blue light-filtering IOL were injected significantly later than patients with an IOL filtering …
View article: The T414G mitochondrial DNA mutation: a biomarker of ageing in human eye
The T414G mitochondrial DNA mutation: a biomarker of ageing in human eye Open
The mitochondrial mutation T414G (mtDNAT414G) has been shown to accumulate in aged and sun-exposed skin. The human eye is also exposed to solar harmful rays. More precisely, the anterior structures of the eye (cornea, iris) filter UV rays …
View article: Apoptosis, the only cell death pathway that can be measured in human diploid dermal fibroblasts following lethal UVB irradiation
Apoptosis, the only cell death pathway that can be measured in human diploid dermal fibroblasts following lethal UVB irradiation Open
Ultraviolet radiation (UVR) is a major environmental genotoxic agent. In skin, it can lead to the formation of mutagenic DNA damage. Several mechanisms are in place to prevent the conversion of these DNA damage into skin cancer-driver muta…
View article: Effect of laser pulse shaping on photoacoustic dosimetry in retinal models
Effect of laser pulse shaping on photoacoustic dosimetry in retinal models Open
Photoacoustic sensing can be a powerful technique to obtain real-time feedback of laser energy dose in treatments of biological tissue. However, when laser therapy uses pulses with microsecond duration, they are not optimal for photoacoust…
View article: Chronology of cellular events related to mitochondrial burnout leading to cell death in Fuchs endothelial corneal dystrophy
Chronology of cellular events related to mitochondrial burnout leading to cell death in Fuchs endothelial corneal dystrophy Open
Fuchs endothelial corneal dystrophy (FECD) is a degenerative eye disease characterized by corneal endothelial cell (CEC) death and the formation of guttae, an abnormal thickening of CEC’s basement membrane. At the tissue level, an oxidativ…
View article: Potential of sub-microsecond laser pulse shaping for controlling microcavitation in selective retinal therapies
Potential of sub-microsecond laser pulse shaping for controlling microcavitation in selective retinal therapies Open
Pilot results showing the potential of sub-microsecond laser pulse shaping to optimize thermomechanical confinement in laser-tissue interactions involving microcavitation are presented. Model samples based on aqueous suspensions of retinal…
View article: Function-Related Protein Expression in Fuchs Endothelial Corneal Dystrophy Cells and Tissue Models
Function-Related Protein Expression in Fuchs Endothelial Corneal Dystrophy Cells and Tissue Models Open
Fuchs endothelial corneal dystrophy (FECD) is a corneal pathology that affects the endothelial cell's ability to maintain deturgescence, resulting in a progressive loss of corneal transparency. In this study, we investigated the expression…
View article: AB044. Phototoxic stress induced in retinal pigmented epithelium cells by the synergy between polycyclic aromatic hydrocarbons and blue light
AB044. Phototoxic stress induced in retinal pigmented epithelium cells by the synergy between polycyclic aromatic hydrocarbons and blue light Open
Background: Lesion to the retinal pigment epithelium (RPE) is a crucial event in age-related macular degeneration (AMD) development. Although the pathogenesis of this complex disease is poorly understood, sunlight exposure and smoking are …
View article: AB093. Ocular tissues bank for vision health research
AB093. Ocular tissues bank for vision health research Open
Background: This infrastructure delivers biological material necessary for several research projects to Vision Health Research Network investigators (VHRN). Methods: Héma-Québec is the organism in charge obtaining consent and retrieving do…
View article: AB024. Photo-oxidation of N-retinylidene-N-retinylethanolamine in vitro by high-energy visible light
AB024. Photo-oxidation of N-retinylidene-N-retinylethanolamine in vitro by high-energy visible light Open
Background: Age-related macular degeneration (AMD) is the second Canadian cause in visual deficiency. AMD is characterized by the death of photoreceptors and retinal pigmented epithelium (RPE) in the macular region of the retina, leading t…
View article: AB047. Quebec database of clinical data and biological material for research on uveal melanoma
AB047. Quebec database of clinical data and biological material for research on uveal melanoma Open
Background: Sharing biological material and clinical data from patients with uveal melanoma. Methods: Uveal melanoma is the most common intraocular malignancy in the adult population. Because uveal melanoma is primarily a sporadic cancer a…