Patrick J. Shilling
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View article: Production of recombinant coiled coil silk proteins for materials synthesis
Production of recombinant coiled coil silk proteins for materials synthesis Open
Rational design of fundamentally new advanced materials would be facilitated by availability of polymers with controlled monomer sequence. Recombinant proteins offer polymers with controlled monomer sequence but are underrepresented in mat…
View article: Tips for efficiently maintaining pET expression plasmids
Tips for efficiently maintaining pET expression plasmids Open
pET expression plasmids are widely used for producing recombinant proteins in Escherichia coli. Selection and maintenance of cells harboring a pET plasmid are possible using either a Tn3.1-type genetic fragment (which encodes a ß-lactamase…
View article: Structural and functional insights into the Pseudomonas aeruginosa glycosyltransferase WaaG and the implications for lipopolysaccharide biosynthesis
Structural and functional insights into the Pseudomonas aeruginosa glycosyltransferase WaaG and the implications for lipopolysaccharide biosynthesis Open
The glycosyltransferase WaaG in Pseudomonas aeruginosa (PaWaaG) is involved in the synthesis of the core region of lipopolysaccharides. It is a promising target for developing adjuvants that could help in the uptake of antibiotics. Herein,…
View article: Signal amplification of <i>araC pBAD</i> using a standardized translation initiation region
Signal amplification of <i>araC pBAD</i> using a standardized translation initiation region Open
araC pBAD is a genetic fragment that regulates the expression of the araBAD operon in bacteria, which is required for the metabolism of L-arabinose. It is widely used in bioengineering applications because it can drive regulatable and titr…
View article: Implementing Novel Designs in pET Expression Plasmids that Increase Protein Production
Implementing Novel Designs in pET Expression Plasmids that Increase Protein Production Open
pET expression plasmids are widely used in the biotechnology, biopharmaceutical, and basic research sectors for the production of recombinant proteins. Typically, they are used off-the-shelf because they support high production titers; how…
View article: Increased production of periplasmic proteins in Escherichia coli by directed evolution of the translation initiation region
Increased production of periplasmic proteins in Escherichia coli by directed evolution of the translation initiation region Open
Background Recombinant proteins are often engineered with an N-terminal signal peptide, which facilitates their secretion to the oxidising environment of the periplasm (gram-negative bacteria) or the culture supernatant (gram-positive bact…
View article: Folding pathway of an Ig domain is conserved on and off the ribosome
Folding pathway of an Ig domain is conserved on and off the ribosome Open
Proteins that fold cotranslationally may do so in a restricted configurational space, due to the volume occupied by the ribosome. How does this environment, coupled with the close proximity of the ribosome, affect the folding pathway of a …
View article: The Folding Pathway of an Ig Domain is Conserved On and Off the Ribosome
The Folding Pathway of an Ig Domain is Conserved On and Off the Ribosome Open
Proteins that fold cotranslationally may do so in a restricted configurational space, due to the volume occupied by the ribosome. How does this environment, coupled with the close proximity of the ribosome, affect the folding pathway of a …
View article: Spatial separation of FtsZ and FtsN during cell division
Spatial separation of FtsZ and FtsN during cell division Open
Summary The division of Escherichia coli is mediated by a collection of some 34 different proteins that are recruited to the division septum and are thought to assemble into a macromolecular complex known as ‘the divisome’. Herein, we have…