Patrick Masson
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View article: Butyrylcholinesterase-Loaded Liposomes and Polymersomes: Catalytic Parameters for Three Types of Substrates
Butyrylcholinesterase-Loaded Liposomes and Polymersomes: Catalytic Parameters for Three Types of Substrates Open
The nano-technological approach and supramolecular chemistry principles relation to the encapsulation of enzymes pave the way for creating next-generation nano-system-functionalized nano-compartments. The most promising approach for prophy…
View article: Kinetic analysis suggests that malathion at low environmental exposures is not toxic to humans
Kinetic analysis suggests that malathion at low environmental exposures is not toxic to humans Open
Malathion has the reputation of being a safe pesticide. There are no reported cases of cholinergic toxicity in people exposed to low environmental doses of malathion. Our goal was to explain the safety of malathion in terms of the mechanis…
View article: New views on physiological functions and regulation of butyrylcholinesterase and potential therapeutic interventions
New views on physiological functions and regulation of butyrylcholinesterase and potential therapeutic interventions Open
Butyrylcholinesterase (BChE) is widely distributed in human tissues, although its physiological roles remain incompletely defined. It contributes modestly to cholinergic transmission and participates in lipid and ghrelin metabolism. BChE i…
View article: Affinity Electrophoresis of Proteins for Determination of Ligand Affinity and Exploration of Binding Sites
Affinity Electrophoresis of Proteins for Determination of Ligand Affinity and Exploration of Binding Sites Open
Affinity gel electrophoresis was introduced about 50 years ago. Proteins interact with a ligand immobilized in the support. Specific interactions cause a decrease in electrophoretic mobility. The presence of a free ligand, competing with a…
View article: Slow-binding inhibitors of enzymes: kinetic characteristics and pharmacological interest
Slow-binding inhibitors of enzymes: kinetic characteristics and pharmacological interest Open
Currently, the search for new slow-binding inhibitors of enzymes (SBI) and their identification primary in vitro studies still attracts much attention in the context of their potential role as putative pharmacological agents for the treatm…
View article: Enzyme-containing double layer polymersomes coated by erythrocytes as a biomimetic nanoscavengers for <i>in vivo</i> protection from toxicants
Enzyme-containing double layer polymersomes coated by erythrocytes as a biomimetic nanoscavengers for <i>in vivo</i> protection from toxicants Open
Microreactors based on enzyme-loaded polymersomes captured by erythrocyte ghosts are promising and open a new way to personalized medical countermeasures for detoxification of organophosphorus compounds and other toxicants.
View article: Polystyrene–Poly(acrylic acid) Block Copolymers for Encapsulation of Butyrylcholinesterase into Injectable Nanoreactors
Polystyrene–Poly(acrylic acid) Block Copolymers for Encapsulation of Butyrylcholinesterase into Injectable Nanoreactors Open
The article is devoted to the creation of enzymatic nanoreactors based on polystyrene–block–poly(acrylic acid) (PS-b-PAA) copolymers containing bioscavengers capable of neutralizing toxic esters both in the body and in the environment. Blo…
View article: Applications of Microbial Organophosphate-Degrading Enzymes to Detoxification of Organophosphorous Compounds for Medical Countermeasures against Poisoning and Environmental Remediation
Applications of Microbial Organophosphate-Degrading Enzymes to Detoxification of Organophosphorous Compounds for Medical Countermeasures against Poisoning and Environmental Remediation Open
Mining of organophosphorous (OPs)-degrading bacterial enzymes in collections of known bacterial strains and in natural biotopes are important research fields that lead to the isolation of novel OP-degrading enzymes. Then, implementation of…
View article: Biodegradable PMMA coated Zn–Mg alloy with bimodal grain structure for orthopedic applications - A promising alternative
Biodegradable PMMA coated Zn–Mg alloy with bimodal grain structure for orthopedic applications - A promising alternative Open
The study examines the impact of microstructure and polymethyl methacrylate (PMMA) grafting on the degradability of Zn-Mg alloys. The mechanical properties of a Zn alloy containing 0.68 wt% Mg and extruded at 200 °C are enhanced for degrad…
View article: Pre-Steady-State and Steady-State Kinetic Analysis of Butyrylcholinesterase-Catalyzed Hydrolysis of Mirabegron, an Arylacylamide Drug
Pre-Steady-State and Steady-State Kinetic Analysis of Butyrylcholinesterase-Catalyzed Hydrolysis of Mirabegron, an Arylacylamide Drug Open
The β-adrenergic drug Mirabegron, a drug initially used for the treatment of an overactive bladder, has new potential indications and is hydrolyzed by butyrylcholinesterase (BChE). This compound is one of the only arylacylamide substrates …
View article: Human Plasma Butyrylcholinesterase Hydrolyzes Atropine: Kinetic and Molecular Modeling Studies
Human Plasma Butyrylcholinesterase Hydrolyzes Atropine: Kinetic and Molecular Modeling Studies Open
The participation of butyrylcholinesterase (BChE) in the degradation of atropine has been recurrently addressed for more than 70 years. However, no conclusive answer has been provided for the human enzyme so far. In the present work, a ste…
View article: Tuning the Envelope Structure of Enzyme Nanoreactors for In Vivo Detoxification of Organophosphates
Tuning the Envelope Structure of Enzyme Nanoreactors for In Vivo Detoxification of Organophosphates Open
Encapsulated phosphotriesterase nanoreactors show their efficacy in the prophylaxis and post-exposure treatment of poisoning by paraoxon. A new enzyme nanoreactor (E-nRs) containing an evolved multiple mutant (L72C/Y97F/Y99F/W263V/I280T) o…
View article: Randomized open-label controlled study of cancer vaccine OSE2101 versus chemotherapy in HLA-A2-positive patients with advanced non-small-cell lung cancer with resistance to immunotherapy: ATALANTE-1
Randomized open-label controlled study of cancer vaccine OSE2101 versus chemotherapy in HLA-A2-positive patients with advanced non-small-cell lung cancer with resistance to immunotherapy: ATALANTE-1 Open
In HLA-A2-positive patients with advanced NSCLC and secondary resistance to immunotherapy, OSE2101 increased survival with better safety compared to CT. Further evaluation in this population is warranted.
View article: Partial Reversible Inhibition of Enzymes and Its Metabolic and Pharmaco-Toxicological Implications
Partial Reversible Inhibition of Enzymes and Its Metabolic and Pharmaco-Toxicological Implications Open
Partial reversible inhibition of enzymes, also called hyperbolic inhibition, is an uncommon mechanism of reversible inhibition, resulting from a productive enzyme–inhibitor complex. This type of inhibition can involve competitive, mixed, n…
View article: Activation/Inhibition of Cholinesterases by Excess Substrate: Interpretation of the Phenomenological b Factor in Steady-State Rate Equation
Activation/Inhibition of Cholinesterases by Excess Substrate: Interpretation of the Phenomenological b Factor in Steady-State Rate Equation Open
Cholinesterases (ChEs) display a non-michaelian behavior with positively charged substrates. In the steady-state rate equation, the b factor describes this behavior: if b > 1 there is substrate activation, if b < 1 there is substrate inhib…
View article: Biomedical Nanosystems for <i>in vivo</i> Detoxification: From Passive Delivery Systems to Functional Nanodevices and Nanorobots
Biomedical Nanosystems for <i>in vivo</i> Detoxification: From Passive Delivery Systems to Functional Nanodevices and Nanorobots Open
The problem of low efficiency of nanotherapeutic drugs challenges the creation of new alternative biomedical nanosystems known as robotic nanodevices. In addition to encapsulating properties, nanodevices can perform different biomedical fu…
View article: Use of a Photocleavable Initiator to Characterize Polymer Chains Grafted onto a Metal Plate with the Grafting-from Method
Use of a Photocleavable Initiator to Characterize Polymer Chains Grafted onto a Metal Plate with the Grafting-from Method Open
The thorough characterization of polymer chains grafted through a “grafting-from” process onto substrates based on the determination of number (Mn) and weight (Mw) average molar masses, as well as dispersity (Ɖ), is quite challenging. It r…
View article: Effect of Mg Addition and PMMA Coating on the Biodegradation Behaviour of Extruded Zn Material
Effect of Mg Addition and PMMA Coating on the Biodegradation Behaviour of Extruded Zn Material Open
Although zinc (Zn) is one of the elements with the greatest potential for biodegradable uses, pure Zn does not have the ideal mechanical or degrading properties for orthopaedic applications. The current research aims at studying the micros…
View article: Quantitative Measurements of Pharmacological and Toxicological Activity of Molecules
Quantitative Measurements of Pharmacological and Toxicological Activity of Molecules Open
Toxicity and pharmacological activity scales of molecules, in particular toxicants, xenobiotics, drugs, nutraceuticals, etc., are described by multiples indicators, and the most popular is the median lethal dose (LD50). At the molecular le…
View article: Conformational Stability and Denaturation Processes of Proteins Investigated by Electrophoresis under Extreme Conditions
Conformational Stability and Denaturation Processes of Proteins Investigated by Electrophoresis under Extreme Conditions Open
The functional structure of proteins results from marginally stable folded conformations. Reversible unfolding, irreversible denaturation, and deterioration can be caused by chemical and physical agents due to changes in the physicochemica…
View article: Kinetic Processes in Enzymatic Nanoreactors for In Vivo Detoxification
Kinetic Processes in Enzymatic Nanoreactors for In Vivo Detoxification Open
Enzymatic nanoreactors are enzyme-encapsulated nanobodies that are capable of performing biosynthetic or catabolic reactions. For this paper, we focused on therapeutic enzyme nanoreactors for the neutralization of toxicants, paying special…
View article: Iron Oxide/Polymer Core–Shell Nanomaterials with Star-like Behavior
Iron Oxide/Polymer Core–Shell Nanomaterials with Star-like Behavior Open
Embedding nanoparticles (NPs) with organic shells is a way to control their aggregation behavior. Using polymers allows reaching relatively high shell thicknesses but suffers from the difficulty of obtaining regular hybrid objects at gram …
View article: Impact of Sucrose as Osmolyte on Molecular Dynamics of Mouse Acetylcholinesterase
Impact of Sucrose as Osmolyte on Molecular Dynamics of Mouse Acetylcholinesterase Open
The enzyme model, mouse acetylcholinesterase, which exhibits its active site at the bottom of a narrow gorge, was investigated in the presence of different concentrations of sucrose to shed light on the protein and water dynamics in cholin…
View article: 1-(3-Tert-Butylphenyl)-2,2,2-Trifluoroethanone as a Potent Transition-State Analogue Slow-Binding Inhibitor of Human Acetylcholinesterase: Kinetic, MD and QM/MM Studies
1-(3-Tert-Butylphenyl)-2,2,2-Trifluoroethanone as a Potent Transition-State Analogue Slow-Binding Inhibitor of Human Acetylcholinesterase: Kinetic, MD and QM/MM Studies Open
Kinetic studies and molecular modeling of human acetylcholinesterase (AChE) inhibition by a fluorinated acetophenone derivative, 1-(3-tert-butylphenyl)-2,2,2-trifluoroethanone (TFK), were performed. Fast reversible inhibition of AChE by TF…
View article: Slow-binding reversible inhibitor of acetylcholinesterase with long-lasting action for prophylaxis of organophosphate poisoning
Slow-binding reversible inhibitor of acetylcholinesterase with long-lasting action for prophylaxis of organophosphate poisoning Open
Organophosphorus (OP) compounds represent a serious health hazard worldwide. The dominant mechanism of their action results from covalent inhibition of acetylcholinesterase (AChE). Standard therapy of acute OP poisoning is partially effect…