Patrick Schindele
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Increasing deletion sizes and the efficiency of <span>CRISPR</span>/Cas9‐mediated mutagenesis by <span>SunTag</span>‐mediated <span>TREX1</span> recruitment Open
SUMMARY Previously, it has been shown that mutagenesis frequencies can be improved by directly fusing the human exonuclease TREX2 to Cas9, resulting in a strong increase in the frequency of smaller deletions at the cut site. Here, we demon…
Optimizing <i>Er</i>Cas12a for efficient gene editing in <i>Arabidopsis thaliana</i> Open
Summary The Er Cas12a nuclease, also known as MAD7, is part of a CRISPR/Cas system from Eubacterium rectale and distantly related to Cas12a nucleases. As it shares only 31% sequence homology with the commonly used As Cas12a, its intellectu…
Enhancing gene editing and gene targeting efficiencies in <i>Arabidopsis thaliana</i> by using an intron‐containing version of <span>tt<i>Lb</i>Cas12a</span> Open
The CRISPR/Cas12a system can be used in a wide range of organisms. However, in plants, efficient editing by Cas12a was hampered due to the lower temperatures required for plant cultivation (Malzahn et al., 2019). Recently, we engineered an…
Massive Crossover Suppression by CRISPR-Cas-mediated Plant Chromosome Engineering Open
Recent studies have demonstrated that not only genes, but also entire chromosomes can be engineered using CRISPR-Cas9 1–5. A major objective of applying chromosome restructuring (CR) in plant breeding is the manipulation of genetic exchang…
Von Genen zu Chromosomen: Pflanzenzüchtung mit CRISPR-CAS Open
Using the CRISPR-Cas system, it has been possible to introduce different kinds of mutations in single or multiple genes for trait improvement in crops. Last year, for the first time, the CRISPR-Cas-mediated induction of different kinds of …
Different DNA repair pathways are involved in single-strand break-induced genomic changes in plants Open
In nature, single-strand breaks (SSBs) in DNA occur more frequently (by orders of magnitude) than double-strand breaks (DSBs). SSBs induced by the CRISPR/Cas9 nickase at a distance of 50–100 bp on opposite strands are highly mutagenic, lea…
Efficient gene targeting in <i>Nicotiana tabacum</i> using CRISPR/SaCas9 and temperature tolerant LbCas12a Open
Summary Nicotiana tabacum is a non‐food herb that has the potential to be utilized as bio‐factory for generating medicines, vaccines or valuable small metabolites. To achieve these goals, the improvement of genetic tools for pre‐designed g…
CRISPR/Cas-mediated chromosome engineering: opening up a new avenue for plant breeding Open
The advent of powerful site-specific nucleases, particularly the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) system, which enables precise genome manipulation, has revolutionized plant…
Using CRISPR/tt<i>Lb</i>Cas12a for <i>in planta</i> Gene Targeting in <i>A. thaliana</i> Open
CRISPR/Cas systems enable gene editing through the induction of site‐specific DNA double‐strand breaks (DSB). However, the nature of the induced modification highly depends on the mechanism used for DNA DSB repair. Non‐homologous end joini…
Application of Aptamers Improves CRISPR-Based Live Imaging of Plant Telomeres Open
Development of live imaging techniques for providing information how chromatin is organized in living cells is pivotal to decipher the regulation of biological processes. Here, we demonstrate the improvement of a live imaging technique bas…
Enhancing <i>in planta</i> gene targeting efficiencies in Arabidopsis using temperature‐tolerant CRISPR/<i>Lb</i>Cas12a Open
Enhancing in planta gene targeting efficiencies in Arabidopsis using temperature-tolerant CRISPR/LbCas12a
Application of aptamers improves CRISPR-based live imaging of plant telomeres Open
Development of live imaging techniques for providing information how chromatin is organized in living cells is pivotal to decipher the regulation of biological processes. Here, we demonstrate the improvement of a live imaging technique bas…
Engineering CRISPR/<i>Lb</i>Cas12a for highly efficient, temperature‐tolerant plant gene editing Open
Engineering CRISPR/Lb Cas12a for highly efficient, temperature‐tolerant plant gene editing
Transforming plant biology and breeding with <span>CRISPR</span>/Cas9, Cas12 and Cas13 Open
Currently, biology is revolutionized by ever growing applications of the CRISPR /Cas system. As discussed in this Review, new avenues have opened up for plant research and breeding by the use of the sequence‐specific DN ases Cas9 and Cas12…
Live‐cell <span>CRISPR</span> imaging in plants reveals dynamic telomere movements Open
Summary Elucidating the spatiotemporal organization of the genome inside the nucleus is imperative to our understanding of the regulation of genes and non‐coding sequences during development and environmental changes. Emerging techniques o…