Patrick Skog
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View article: Deep learning-enabled scaffolding of spatial arrays of PfCSP epitopes
Deep learning-enabled scaffolding of spatial arrays of PfCSP epitopes Open
Malaria is a leading cause of disease in developing countries. The licensed malaria vaccine RTS,S/AS01 confers partial protection in part due to the elicitation of circumsporozoite protein (CSP) antibodies, of which those to the CSP repeat…
View article: Virus glycoprotein nanodisc platform for vaccine design
Virus glycoprotein nanodisc platform for vaccine design Open
Transmembrane glycoproteins of enveloped viruses are the targets of neutralizing antibodies and essential vaccine antigens. mRNA-LNP technology allows in situ production of transmembrane glycoproteins upon immunization, but biophysical cha…
View article: Heterologous prime-boost vaccination drives early maturation of HIV broadly neutralizing antibody precursors in humanized mice
Heterologous prime-boost vaccination drives early maturation of HIV broadly neutralizing antibody precursors in humanized mice Open
A protective HIV vaccine will likely need to induce broadly neutralizing antibodies (bnAbs). Vaccination with the germline-targeting immunogen eOD-GT8 60mer adjuvanted with AS01 B was found to induce VRC01-class bnAb precursors in 97% of v…
View article: Efficient isolation of rare B cells using next-generation antigen barcoding
Efficient isolation of rare B cells using next-generation antigen barcoding Open
The ability to efficiently isolate antigen-specific B cells in high throughput will greatly accelerate the discovery of therapeutic monoclonal antibodies (mAbs) and catalyze rational vaccine development. Traditional mAb discovery is a cost…
View article: Efficient isolation of rare B cells using next-generation antigen barcoding
Efficient isolation of rare B cells using next-generation antigen barcoding Open
The ability to efficiently isolate antigen-specific B cells in high throughput will greatly accelerate the discovery of therapeutic monoclonal antibodies ( mAbs ) and catalyze rational vaccine development. Traditional mAb discovery is a co…
View article: B cells expressing authentic naive human VRC01-class BCRs can be recruited to germinal centers and affinity mature in multiple independent mouse models
B cells expressing authentic naive human VRC01-class BCRs can be recruited to germinal centers and affinity mature in multiple independent mouse models Open
Significance Rational development of successful vaccines requires utilization of predictive models of vaccination. One approach for development of an HIV vaccine has been to study broadly neutralizing antibodies (bnAbs) and revert the muta…
View article: B cells expressing authentic naive human VRC01-class BCRs can be primed and recruited to germinal centers in multiple independent mouse models
B cells expressing authentic naive human VRC01-class BCRs can be primed and recruited to germinal centers in multiple independent mouse models Open
Animal models of human antigen-specific B cell receptors (BCR) generally depend on “inferred germline” sequences, and thus their relationship to authentic naive human B cell BCR sequences and affinities is unclear. Here, BCR sequences from…
View article: Detection and activation of HIV broadly neutralizing antibody precursor B cells using anti-idiotypes
Detection and activation of HIV broadly neutralizing antibody precursor B cells using anti-idiotypes Open
Many tested vaccines fail to provide protection against disease despite the induction of antibodies that bind the pathogen of interest. In light of this, there is much interest in rationally designed subunit vaccines that direct the antibo…
View article: Design and crystal structure of a native-like HIV-1 envelope trimer that engages multiple broadly neutralizing antibody precursors in vivo
Design and crystal structure of a native-like HIV-1 envelope trimer that engages multiple broadly neutralizing antibody precursors in vivo Open
Induction of broadly neutralizing antibodies (bNAbs) by HIV-1 envelope glycoprotein immunogens would be a major advance toward an effective vaccine. A critical step in this process is the activation of naive B cells expressing germline (gl…