Patrick Wuchter
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View article: <scp>SARS</scp> ‐ <scp>CoV</scp> ‐2 convalescent plasma for the controlled clinical trial “ <scp>COVIC</scp> ‐19”: Experience from collection of very high‐titer plasma from superimmunized individuals
<span>SARS</span> ‐ <span>CoV</span> ‐2 convalescent plasma for the controlled clinical trial “ <span>COVIC</span> ‐19”: Experience from collection of very high‐titer plasma from superimmunized individuals Open
Background COVID‐19 convalescent plasma (CCP) is a treatment option for COVID‐19. Understanding of donor and product characteristics is important for optimization of CCP therapy. We report the experience of collection of very high‐titer CC…
View article: Effects of tumor treating fields (TTFields) on human mesenchymal stromal cells
Effects of tumor treating fields (TTFields) on human mesenchymal stromal cells Open
View article: Prolonged Thrombocytopenia and Severe Transfusion Reaction after ABO-Incompatible Allogeneic Hematopoietic Stem Cell Transplantation in a Patient with Chronic Myelomonocytic Leukemia
Prolonged Thrombocytopenia and Severe Transfusion Reaction after ABO-Incompatible Allogeneic Hematopoietic Stem Cell Transplantation in a Patient with Chronic Myelomonocytic Leukemia Open
Introduction: Major ABO-incompatible allogeneic hematopoietic stem cell transplantation (allo-HCT) is a common practice and represents a challenging transfusion scenario. Prolonged thrombocytopenia with increased platelet transfusion needs…
View article: Processing, Cryopreserving, and Controlling the Quality of HSC
Processing, Cryopreserving, and Controlling the Quality of HSC Open
The efficiency of an autologous, as well as an allogeneic, HSC graft is mainly determined by the number of CD34 + cells present. The dose of transplanted CD34 + cells per kg body weight (bw) determines the kinetics of the neutrophil and pl…
View article: Supplementary Material for: Flow cytometric characterization of HSPC subpopulations in autologous PBSC preparations after cryopreservation
Supplementary Material for: Flow cytometric characterization of HSPC subpopulations in autologous PBSC preparations after cryopreservation Open
Introduction: Autologous stem cell transplantation is a successful routine procedure with only a small number of non-engraftment cases, although the time to hematopoietic recovery may vary considerably across patients. While CD34 has been …
View article: Mobilization and Collection of HSC
Mobilization and Collection of HSC Open
The intravenous infusion of patient’s own HSC (autologous SCT) to restore BM damage is the basic principle of high-dose chemotherapy, since otherwise the patient would expect long-lasting aplasia with life-threatening infections. Therefore…
View article: <i>ASXL1</i> mutations are associated with a response to alvocidib and 5-azacytidine combination in myelodysplastic neoplasms
<i>ASXL1</i> mutations are associated with a response to alvocidib and 5-azacytidine combination in myelodysplastic neoplasms Open
Inhibitors of anti-apoptotic BCL-2 family proteins in combination with chemotherapy and hypomethylating agents (HMAs) are promising therapeutic approaches in acute myeloid leukemia (AML) and high-risk myelodysplastic syndromes (MDS). Alvoc…
View article: Treatment of adult ALL patients with third-generation CD19-directed CAR T cells: results of a pivotal trial
Treatment of adult ALL patients with third-generation CD19-directed CAR T cells: results of a pivotal trial Open
Background Third-generation chimeric antigen receptor (CAR)-engineered T cells (CARTs) might improve clinical outcome of patients with B cell malignancies. This is the first report on a third-generation CART dose-escalating, phase-1/2 inve…
View article: SARS-CoV-2 vaccination of convalescents boosts neutralization capacity against Omicron subvariants BA.1, BA.2 and BA.5 and can be predicted by anti-S antibody concentrations in serological assays
SARS-CoV-2 vaccination of convalescents boosts neutralization capacity against Omicron subvariants BA.1, BA.2 and BA.5 and can be predicted by anti-S antibody concentrations in serological assays Open
Background Recent data on immune evasion of new SARS-CoV-2 variants raise concerns about the efficacy of antibody-based COVID-19 therapies. Therefore, in this study the in-vitro neutralization capacity against SARS-CoV-2 variant B.1 and th…
View article: Inhibition of lysyl oxidases synergizes with 5-azacytidine to restore erythropoiesis in myelodysplastic and myeloid malignancies
Inhibition of lysyl oxidases synergizes with 5-azacytidine to restore erythropoiesis in myelodysplastic and myeloid malignancies Open
Limited response rates and frequent relapses during standard of care with hypomethylating agents in myelodysplastic neoplasms (MN) require urgent improvement of this treatment indication. Here, by combining 5-azacytidine (5-AZA) with the p…
View article: Flow Cytometric Characterization of Hematopoietic Stem and Progenitor Cell Subpopulations in Autologous Peripheral Blood Stem Cell Preparations after Cryopreservation
Flow Cytometric Characterization of Hematopoietic Stem and Progenitor Cell Subpopulations in Autologous Peripheral Blood Stem Cell Preparations after Cryopreservation Open
Introduction: Autologous stem cell transplantation is a successful routine procedure with only a small number of non-engraftment cases, although the time to hematopoietic recovery may vary considerably across patients. While CD34 has been …
View article: Stem Cell Mobilization, Collection, and Processing
Stem Cell Mobilization, Collection, and Processing Open
View article: Predicting Successful Hematopoietic Stem Cell Collection in Healthy Allogeneic Donors
Predicting Successful Hematopoietic Stem Cell Collection in Healthy Allogeneic Donors Open
Introduction: Collection of peripheral blood stem cells (PBSCs) from healthy donors is a well-established process. We aimed to identify factors predictive of successful CD34+ PBSC collection and established a formula capable of predicting …
View article: Human Mesenchymal Stromal Cells Do Not Cause Radioprotection of Head-and-Neck Squamous Cell Carcinoma
Human Mesenchymal Stromal Cells Do Not Cause Radioprotection of Head-and-Neck Squamous Cell Carcinoma Open
Radiotherapy of head-and-neck squamous cell carcinoma (HNSCC) can cause considerable normal tissue injuries, and mesenchymal stromal cells (MSCs) have been shown to aid regeneration of irradiation-damaged normal tissues. However, utilizati…
View article: Increased plasma level of soluble P-selectin in non-hospitalized COVID-19 convalescent donors
Increased plasma level of soluble P-selectin in non-hospitalized COVID-19 convalescent donors Open
View article: SARS-CoV-2 vaccination of convalescents boosts neutralization capacity against SARS-CoV-2 Delta and Omicron that can be predicted by anti-S antibody concentrations in serological assays
SARS-CoV-2 vaccination of convalescents boosts neutralization capacity against SARS-CoV-2 Delta and Omicron that can be predicted by anti-S antibody concentrations in serological assays Open
Background Recent data on immune evasion of new SARS-CoV-2 variants raise concerns about antibody-based COVID-19 therapies. Therefore in this study the in-vitro neutralization capacity against SARS-CoV-2 variants Wuhan D614G, Delta and Omi…
View article: Closer to Nature: The Role of MSCs in Recreating the Microenvironment of the Hematopoietic Stem Cell Niche in vitro
Closer to Nature: The Role of MSCs in Recreating the Microenvironment of the Hematopoietic Stem Cell Niche in vitro Open
Background: The stem cell niche in human bone marrow provides scaffolds, cellular frameworks and essential soluble cues to support the stemness of hematopoietic stem and progenitor cells (HSPCs). To decipher this complex stru…
View article: Local Radiation Therapy Before and During Induction Delays Stem Cell Mobilization and Collection in Multiple Myeloma Patients
Local Radiation Therapy Before and During Induction Delays Stem Cell Mobilization and Collection in Multiple Myeloma Patients Open
In multiple myeloma, local radiation therapy (RT) of osseous lesions before peripheral blood stem cell (PBSC) mobilization is assumed to impair the PBSC mobilization and collection. However, the results of previously published studies are …
View article: Autoantibodies against Progranulin and IL-1 receptor antagonist due to immunogenic posttranslational isoforms contribute to hyperinflammation in critically ill COVID-19
Autoantibodies against Progranulin and IL-1 receptor antagonist due to immunogenic posttranslational isoforms contribute to hyperinflammation in critically ill COVID-19 Open
STRUCTURED ABSTRACT Hyperinflammation is frequently observed in patients with severe COVID-19. Inadequate and defective IFN type I responses against SARS-CoV-2, associated with autoantibodies in a proportion of patients, lead to severe cou…
View article: Supplementary Material for: Donors for SARS-CoV-2 Convalescent Plasma for a Controlled Clinical Trial: Donor Characteristics, Content and Time Course of SARS-CoV-2 Neutralizing Antibodies
Supplementary Material for: Donors for SARS-CoV-2 Convalescent Plasma for a Controlled Clinical Trial: Donor Characteristics, Content and Time Course of SARS-CoV-2 Neutralizing Antibodies Open
Background: Convalescent plasma is one of the treatment options for COVID-19 which is currently being investigated in many clinical trials. Understanding of donor and product characteristics is important for optimization of c…
View article: Donors for SARS-CoV-2 Convalescent Plasma for a Controlled Clinical Trial: Donor Characteristics, Content and Time Course of SARS-CoV-2 Neutralizing Antibodies
Donors for SARS-CoV-2 Convalescent Plasma for a Controlled Clinical Trial: Donor Characteristics, Content and Time Course of SARS-CoV-2 Neutralizing Antibodies Open
Background: Convalescent plasma is one of the treatment options for COVID-19 which is currently being investigated in many clinical trials. Understanding of donor and product characteristics is important for optimization of c…
View article: Cryostorage to What End? – Autologous Stem Cell Products in Burkitt Lymphoma, Acute Lymphoblastic Leukemia, Acute Myeloid Leukemia, and Myeloproliferative Neoplasm Patients
Cryostorage to What End? – Autologous Stem Cell Products in Burkitt Lymphoma, Acute Lymphoblastic Leukemia, Acute Myeloid Leukemia, and Myeloproliferative Neoplasm Patients Open
Introduction: Recently, we identified a huge discrepancy between the collection practice and the actual utilization of cryopreserved peripheral blood stem cells (PBSCs) for high-dose chemotherapy (HDCT) and autologous blood s…
View article: Replication stress signaling is a therapeutic target in myelodysplastic syndromes with splicing factor mutations
Replication stress signaling is a therapeutic target in myelodysplastic syndromes with splicing factor mutations Open
Somatic mutations in genes coding for splicing factors, e.g., SF3B1, U2AF1, SRSF2, and others are found in approximately 50% of patients with myelodysplastic syndromes (MDS). These mutations have been predicted to frequently occur early in…
View article: Platelet storage and functional integrity
Platelet storage and functional integrity Open
Platelet transfusion is a topic of common interest for many specialists involved in patient care, from laboratory staff to clinical physicians. Various aspects make this type of transfusion different from those of other blood components. I…
View article: Correction: High-risk additional chromosomal abnormalities at low blast counts herald death by CML
Correction: High-risk additional chromosomal abnormalities at low blast counts herald death by CML Open
View article: Storage, Utilization, and Disposal of Hematopoietic Stem Cell Products in Patients with Multiple Myeloma
Storage, Utilization, and Disposal of Hematopoietic Stem Cell Products in Patients with Multiple Myeloma Open
High-dose chemotherapy (HD-CHT) and autologous blood stem cell transplantation (ABSCT) represent the standard of care in multiple myeloma (MM) for transplantation-eligible patients. Up to 3 HD-CHT/ABSCT treatments may be administered durin…
View article: High-risk additional chromosomal abnormalities at low blast counts herald death by CML
High-risk additional chromosomal abnormalities at low blast counts herald death by CML Open
View article: Low-dose peripheral blood stem cell graft after high-dose chemotherapy - an evaluation of hematopoietic reconstitution
Low-dose peripheral blood stem cell graft after high-dose chemotherapy - an evaluation of hematopoietic reconstitution Open
View article: Serological hepatitis B virus (HBV) activity in patients with HBV infection and B‐cell non‐Hodgkin’s lymphoma
Serological hepatitis B virus (HBV) activity in patients with HBV infection and B‐cell non‐Hodgkin’s lymphoma Open
Background Previous epidemiological studies suggest an association between hepatitis B virus (HBV) infection and B‐cell non‐Hodgkin lymphoma (B‐NHL). The aim of our study was to evaluate clinical characteristics and serological indicators …
View article: Multipotent mesenchymal stromal cells are sensitive to thermic stress – potential implications for therapeutic hyperthermia
Multipotent mesenchymal stromal cells are sensitive to thermic stress – potential implications for therapeutic hyperthermia Open
Purpose: Hyperthermia demonstrated clinical efficacy in multimodal cancer treatment. Multipotent mesenchymal stromal cells (MSCs) as part of the tumor-supporting stroma modulate tumor response and tissue regeneration after hyperther…