Explanipedia

Targeting Langerhans cells using a modular mannosylated nucleic acid-based vaccine platform Open

Simon Christian Vinther, Antonia Resag, Khoa Le, Claudia Zelle‐Rieser, Helen Strandt , et al. · 2025

The skin is a diverse reservoir of immune cells with strong potential for immunotherapeutic delivery. Langerhans cells (LCs) in the epidermis are antigen-presenting cells, accessible for vaccination using carbohydrate-conjugated therapeuti…

Blocking of IL‐4/IL‐13 Signalling With Dupilumab Results in Restoration of Serum and Cutaneous Abnormalities in Netherton Syndrome Open

Stefan Blunder, Natascha Hermann‐Kleiter, Rita Mahmuti, Martin Hermann, Daniela Ortner , et al. · 2025

Netherton syndrome (NS) is a rare ichthyosis caused by SPINK5‐ null mutations, resulting in erythroderma, ichthyosis linearis circumflexa, and atopic diathesis. Elevated serum IgE levels and activation of the KLK5‐PAR2‐TSLP axis suggest in…

Multi-Epitope DC Vaccines with Melanoma Antigens for Immunotherapy of Melanoma Open

Athanasios Seretis, Lukas Amon, Christoph H. Tripp, Giuseppe Cappellano, Florian Hornsteiner , et al. · 2025

Background/Objectives: The revolution for the treatment of melanoma came with the approval of checkpoint blockade antibodies. However, a substantial proportion of patients show primary or secondary resistance to this type of immunotherapy,…

Regulation of NK cell development, maturation, and antitumor responses by the nuclear receptor NR2F6 Open

Johannes Woelk, Florian Hornsteiner, Stephanie Aschauer-Wallner, Patrizia Stoitzner, Gottfried Baier , et al. · 2025

Single-Cell Integration and Multi-Modal Profiling Reveals Phenotypes and Spatial Organization of Neutrophils in Colorectal Cancer Open

Valentin Marteau, Niloofar Nemati, Kristina Handler, Deeksha Raju, Alexander Kirchmair , et al. · 2025

Guidelines for preparation and flow cytometry analysis of human nonlymphoid tissue DC Open

Diana Dudziak, Lukas Heger, William W. Agace, Joyce Bakker, Tanja D. de Gruijl , et al. · 2024

This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state‐of‐the‐art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional …

Langerhans cells orchestrate apoptosis of DNA‐damaged keratinocytes upon high‐dose UVB skin exposure Open

Daniela Ortner, Helen Strandt, Christoph H. Tripp, Sarah Spoeck, Athanasios Seretis , et al. · 2024

Ultraviolet (UV) irradiation of the skin causes mutations that can promote the development of melanoma and nonmelanoma skin cancer. High‐dose UVB exposure triggers a vigorous skin reaction characterized by inflammation resulting in acute s…

Single-cell integration and multi-modal profiling reveals phenotypes and spatial organization of neutrophils in colorectal cancer Open

Valentin Marteau, Niloofar Nemati, Kristina Handler, Deeksha Raju, Alexander Kirchmair , et al. · 2024

SUMMARY The immune composition of the tumor microenvironment (TME) has a major impact on the therapeutic response and clinical outcome in patients with colorectal cancer (CRC). Here, we comprehensively characterize the TME at the single-ce…

Tumor-targeted therapy with BRAF-inhibitor recruits activated dendritic cells to promote tumor immunity in melanoma Open

Florian Hornsteiner, Janine Vierthaler, Helen Strandt, Antonia Resag, Zhe Fu , et al. · 2024

Background Tumor-targeted therapy causes impressive tumor regression, but the emergence of resistance limits long-term survival benefits in patients. Little information is available on the role of the myeloid cell network, especially dendr…

Dendritic cell-targeted therapy expands CD8 T cell responses to bona-fide neoantigens in lung tumors Open

Lucía López, Luciano Gastón Morosi, Federica La Terza, Pierre Bourdely, Giuseppe Rospo , et al. · 2024

DCs targeted therapy expands CD8 T cell responses to bona-fide neoantigens in lung tumors Open

Lucía López‐Rodríguez, Luciano Gastón Morosi, Federica La Terza, Pierre Bourdely, Giuseppe Rospo , et al. · 2024

Cross-presentation by type 1 cDCs (cDC1) is critical to induce and sustain antitumoral CD8 T cell responses to model antigens, in various tumor settings. However, the impact of cross-presenting cDC1 and the potential of DC-based therapies …

169 Unraveling the role of CD64+ DC population in melanoma Open

J. Vierthaler, Florian Hornsteiner, Helen Strandt, Sophie Dieckmann, Chelsea Tripp , et al. · 2023

Preadipocytes in human granulation tissue: role in wound healing and response to macrophage polarization Open

Tina Rauchenwald, Florian Handle, Catherine Connolly, Antonia Degen, Christof Seifarth , et al. · 2023

DC targeted therapy breaks immune tolerance against neoantigens in refractory lung adenocarcinoma Open

Lucía López, Pierre Bourdely, Federica La Terza, Giuseppe Rospo, Luciano Gastón Morosi , et al. · 2023

The efficacy of immune checkpoint blockade (ICB) in NSCLC depends on the tumor mutational burden (TMB). However, a fraction of patients with high TMB and predicted immunogenic neoantigens (neoAgs) do not respond. Here we show that in a mod…

Guidelines for mouse and human DC functional assays Open

Björn E. Clausen, Lukas Amon, Ronald A. Backer, Luciana Berod, Tobias Bopp , et al. · 2022

This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state‐of‐the‐art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional …

Guidelines for DC preparation and flow cytometry analysis of mouse nonlymphoid tissues Open

Hans Christian Probst, Patrizia Stoitzner, Lukas Amon, Ronald A. Backer, Anna Brand , et al. · 2022

This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state‐of‐the‐art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy and functional c…

Contents: Eur. J. Immunol. 12'22 Open

Lorenz Kretschmer, Tal Pecht, Katja Knapp, Sabrina B. Bennstein, Niclas Flosdorf , et al. · 2022

Mouse dendritic cells and other myeloid subtypes in healthy lymph nodes and skin: 26‐Color flow cytometry panel for immune phenotyping Open

Florian Hornsteiner, Martina Sykora, Christoph H. Tripp, Sieghart Sopper, Patrizia Stoitzner · 2022

This novel 26-color flow cytometry panel allows the detailed immune phenotyping of the complex network of myeloid cells in murine lymph nodes and skin. With the optimized panel the different murine DC-subsets and other myeloid cell types c…

Antigen targeting to dendritic cells: Still a place in future immunotherapy? Open

Patrizia Stoitzner, Nikolaus Romani, Christoph Rademacher, Hans Christian Probst, Karsten Mahnke · 2022

The hallmark of DCs is their potent and outstanding capacity to activate naive resting T cells. As such, DCs are the sentinels of the immune system and instrumental for the induction of immune responses. This is one of the reasons, why DCs…

Early antitumor activity of oral Langerhans cells is compromised by a carcinogen Open

Yasmin Saba, Itay Aizenbud, Daniela Matanes, Noam Koren, Or Barel , et al. · 2022

Significance Oral cancer is often diagnosed at late stages, resulting in a poor prognosis of this disease. Understanding early carcinogenic events taking place during the development of oral cancer is, therefore, an important unmet clinica…

Targeted delivery of a vaccine protein to Langerhans cells in the human skin via the C‐type lectin receptor Langerin Open

Lydia Bellmann, Helen Strandt, Claudia Zelle‐Rieser, Daniela Ortner, Christoph H. Tripp , et al. · 2021

Human skin is a preferred vaccination site as it harbors multiple dendritic cell (DC) subsets, which display distinct C‐type lectin receptors (CLR) that recognize pathogens. Antigens can be delivered to CLR by antibodies or ligands to boos…

Author response for "Targeted delivery of a vaccine protein to Langerhans cells in the human skin via the C‐type lectin receptor Langerin" Open

Lydia Bellmann, Helen Strandt, Claudia Zelle‐Rieser, Daniela Ortner, Christoph H. Tripp , et al. · 2021

Specific Protein Antigen Delivery to Human Langerhans Cells in Intact Skin Open

Mareike Rentzsch, Robert Wawrzinek, Claudia Zelle‐Rieser, Helen Strandt, Lydia Bellmann , et al. · 2021

Immune modulating therapies and vaccines are in high demand, not least to the recent global spread of SARS-CoV2. To achieve efficient activation of the immune system, professional antigen presenting cells have proven to be key coordinators…

Laser‐assisted epicutaneous immunization to target human skin dendritic cells Open

Christoph H. Tripp, Hermann Voit, Angela An, Magdalena Seidl‐Philipp, Johanna Krapf , et al. · 2021

Dendritic cells (DC) are promising targets for immunotherapy of cancer. Clinically, immunization against cancer antigens by means of the most potent antigen‐presenting cells, that is DC, remains an important treatment option in combination…

Differential infection of murine and human dendritic cell subsets by oncolytic vesicular stomatitis virus variants Open

Lisa Pipperger, Lydia Riepler, Janine Kimpel, Anita Siller, Patrizia Stoitzner , et al. · 2021

Oncolytic viruses (OVs) can eradicate tumor cells and elicit antitumor immunity. VSV-GP, a chimeric vesicular stomatitis virus (VSV) with the glycoprotein (GP) of the lymphocytic choriomeningitis virus, is a promising new OV candidate. How…

Skin dendritic cells in melanoma are key for successful checkpoint blockade therapy Open

Natasa Prokopi, Christoph H. Tripp, Bart Tummers, Florian Hornsteiner, Sarah Spoeck , et al. · 2021

Background Immunotherapy with checkpoint inhibitors has shown impressive results in patients with melanoma, but still many do not benefit from this line of treatment. A lack of tumor-infiltrating T cells is a common reason for therapy fail…

BRAF and MEK inhibition in melanoma patients enables reprogramming of tumor infiltrating lymphocytes Open

Lukas Peiffer, Farnoush Farahpour, Ashwin Sriram, Ivelina Spassova, Daniel Hoffmann , et al. · 2020

Background Combined inhibition of BRAF/MEK is an established therapy for melanoma. In addition to its canonical mode of action, effects of BRAF/MEK inhibitors on antitumor immune responses are emerging. Thus, we investigated the effect of …

Combining chemotherapy and autologous peptide‐pulsed dendritic cells provides survival benefit in stage IV melanoma patients Open

Klaus Eisendle, Georg Weinlich, Susanne Ebner, Markus Forstner, Daniela Reider , et al. · 2020

Summary Background and Objectives We examined retrospectively whether the combination of standard dacarbazine (DTIC) and/or fotemustine chemotherapy and autologous peptide‐loaded dendritic cell (DC) vaccination may improve survival of stag…

Kombination von Chemotherapie und autologen, Peptid‐beladenen dendritischen Zellen bringt Überlebensvorteil bei Melanompatienten im Stadium IV Open

Klaus Eisendle, Georg Weinlich, Susanne Ebner, Markus Forstner, Daniela Reider , et al. · 2020

Zusammenfassung Hintergrund und Studienziele Wir untersuchten retrospektiv, ob die Kombination der Standardchemotherapie (Dacarbazin [DTIC] und/oder Fotemustin) und Vakzination mit autologen, Tumorpeptid‐beladenen dendritischen Zellen (DZ)…

539 High dimensional analysis of the human lymph node during melanoma progression reveals shifts in myeloid content that relate to differential T cell content Open

Anastasia Prokopi, Marta Lopez-Gonzalez, Jan Verhoeff, Rieneke van de Ven, Juan J. García‐Vallejo , et al. · 2020

Background The sentinel lymph node (SLN) in melanoma represents the crossroads of the initiation of effector T cell responses and of lymphatic metastasis of the primary tumor. As such, alterations in the human LN immune cell network during…

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