Paul E. Framson
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View article: Expression and Characterization of Murine Hevin (SC1), a Member of the SPARC Family of Matricellular Proteins
Expression and Characterization of Murine Hevin (SC1), a Member of the SPARC Family of Matricellular Proteins Open
Hevin, also known as SC1, MAST 9, SPARC-like 1, RAGS1 and ECM2, is a member of the SPARC-related family of matricellular proteins. Mouse hevin is 53% identical to mouse SPARC, and both proteins share a follistatin-like module and an extrac…
View article: Highly polarized HLA class II antigen processing and presentation by human intestinal epithelial cells.
Highly polarized HLA class II antigen processing and presentation by human intestinal epithelial cells. Open
The high concentration of foreign antigen in the lumen of the gastrointestinal tract is separated from the underlying lymphocytes by a single cell layer of polarized epithelium. Intestinal epithelial cells can express HLA class II antigens…
View article: New genetic techniques for group B streptococci: high-efficiency transformation, maintenance of temperature-sensitive pWV01 plasmids, and mutagenesis with Tn917
New genetic techniques for group B streptococci: high-efficiency transformation, maintenance of temperature-sensitive pWV01 plasmids, and mutagenesis with Tn917 Open
Three techniques were developed to improve the genetic manipulation of group B streptococci (GBS). We first optimized a protocol for transformation of GBS by electroporation, which provided transformation efficiencies of 10(5) CFU/microgra…
View article: Intestinal epithelial cells use two distinct pathways for HLA class II antigen processing.
Intestinal epithelial cells use two distinct pathways for HLA class II antigen processing. Open
Intestinal epithelial cells express a low level of HLA class II molecules constitutively, with elevated levels seen in the setting of mucosal inflammation including inflammatory bowel disease. The ability of intestinal epithelial cells to …
View article: Group B streptococcal beta-hemolysin expression is associated with injury of lung epithelial cells
Group B streptococcal beta-hemolysin expression is associated with injury of lung epithelial cells Open
Group B streptococci (GBS) are the leading cause of serious bacterial infection in newborns. Early-onset disease is heralded by pneumonia and lung injury, and the lung may serve as a portal of entry for GBS into the bloodstream. To examine…
View article: A serum response element and a binding site for NF-Y mediate the serum response of the human thrombospondin 1 gene.
A serum response element and a binding site for NF-Y mediate the serum response of the human thrombospondin 1 gene. Open
The expression of thrombospondin 1 (TSP 1), a member of the TSP gene family, is rapidly induced by growth factors. We tested the ability of human TSP 1-chloramphenicol acetyltransferase constructs to respond to serum in stably transfected …
View article: Characterization of the mouse thrombospondin gene and evaluation of the role of the first intron in human gene expression.
Characterization of the mouse thrombospondin gene and evaluation of the role of the first intron in human gene expression. Open
We have isolated the mouse thrombospondin (TS) gene and determined the DNA sequence of the first nine exons and eight introns. Comparison with the human cDNA sequence reveals a high degree of conservation in coding sequences. Exon 3 of the…
View article: Structural analysis and expression of the human thrombospondin gene promoter.
Structural analysis and expression of the human thrombospondin gene promoter. Open
We have isolated a 34-kilobase pair genomic thrombospondin clone and determined the sequence of 2033 base pairs of the 5′-flanking region, the first entirely untranslated exon and the first intron. A number of interesting regulatory motifs…
View article: Fine structure of the human hypoxanthine phosphoribosyltransferase gene.
Fine structure of the human hypoxanthine phosphoribosyltransferase gene. Open
The human hypoxanthine phosphoribosyltransferase (HPRT) gene has been characterized by molecular cloning, mapping, and DNA sequencing techniques. The entire gene, which is about 44 kilobases in length, is composed of nine exon elements. Th…