Paul E. Verslues
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View article: What Large Language Models Know About Plant Molecular Biology
What Large Language Models Know About Plant Molecular Biology Open
Large language models (LLMs) are rapidly permeating scientific research, yet their capabilities in plant molecular biology remain largely uncharacterized. Here, we present M o B i P lant , the first comprehensive benchmark for evaluating L…
View article: Nonphototrophic hypocotyl 3 domain proteins: traffic directors, hitchhikers, or both?
Nonphototrophic hypocotyl 3 domain proteins: traffic directors, hitchhikers, or both? Open
Summary The nonphototrophic hypocotyl 3 (NPH3) domain is plant specific and of unknown function. It is nearly always attached to an N‐terminal BTB domain and a largely unstructured C‐terminal region. Recent reports revealed NPH3‐domain GTP…
View article: The nonphototrophic hypocotyl 3 (NPH3) domain protein NRL5 is a trafficking-associated GTPase essential for drought resistance
The nonphototrophic hypocotyl 3 (NPH3) domain protein NRL5 is a trafficking-associated GTPase essential for drought resistance Open
The mechanisms of plant drought resistance are unclear but may involve membrane trafficking and metabolic reprogramming, including proline accumulation. Forward genetic screening using a proline dehydrogenase 1 ( ProDH1 ) promoter:reporter…
View article: Please, carefully, pass the P5C
Please, carefully, pass the P5C Open
This article comments on: Zheng Y, Cabassa-Hourton C, Eubel H, Chevreux G, Lignieres L, Crilat E, Braun H-P, Lebreton S, Savouré A. 2024. Pyrroline-5-carboxylate metabolism protein complex detected in Arabidopsis thaliana leaf mitochondria…
View article: AT Hook‐Like 10 phosphorylation determines ribosomal RNA processing 6‐like 1 (RRP6L1) chromatin association and growth suppression during water stress
AT Hook‐Like 10 phosphorylation determines ribosomal RNA processing 6‐like 1 (RRP6L1) chromatin association and growth suppression during water stress Open
Phosphorylation of AT Hook‐Like 10 (AHL10), one of the AT‐hook family of plant‐specific DNA binding proteins, is critical for growth suppression during moderate severity drought (low water potential, ψ w ) stress. To understand how AHL10 p…
View article: The Non-Phototrophic Hypocotyl3 (NPH3)-domain protein NRL5 is a trafficking-associated GTPase essential for drought resistance
The Non-Phototrophic Hypocotyl3 (NPH3)-domain protein NRL5 is a trafficking-associated GTPase essential for drought resistance Open
The mechanisms of plant resistance to low water potential (ψ w ) during drought are unclear but may involve signaling and trafficking at the plasma membrane as well as metabolic reprogramming, including proline accumulation. Forward geneti…
View article: AHL10 phosphorylation determines RRP6L1 chromatin association and growth suppression during water stress
AHL10 phosphorylation determines RRP6L1 chromatin association and growth suppression during water stress Open
Phosphorylation of AHL10, one of the AT-hook family of plant-specific DNA binding proteins, is critical for growth suppression during moderate severity drought (low water potential, ψ w ) stress. To understand how AHL10 phosphorylation det…
View article: Time for a drought experiment: Do you know your plants’ water status?
Time for a drought experiment: Do you know your plants’ water status? Open
Drought stress is an increasing concern because of climate change and increasing demands on water for agriculture. There are still many unknowns about how plants sense and respond to water limitation, including which genes and cellular mec…
View article: Burning questions for a warming and changing world: 15 unknowns in plant abiotic stress
Burning questions for a warming and changing world: 15 unknowns in plant abiotic stress Open
We present unresolved questions in plant abiotic stress biology as posed by 15 research groups with expertise spanning eco-physiology to cell and molecular biology. Common themes of these questions include the need to better understand how…
View article: Natural variation identifies new effectors of water-use efficiency in <i>Arabidopsis</i>
Natural variation identifies new effectors of water-use efficiency in <i>Arabidopsis</i> Open
Water-use efficiency (WUE) is the ratio of biomass produced per unit of water consumed; thus, it can be altered by genetic factors that affect either side of the ratio. In the present study, we exploited natural variation for WUE to discov…
View article: Natural variation identifies new effectors of water use efficiency in<i>Arabidopsis</i>
Natural variation identifies new effectors of water use efficiency in<i>Arabidopsis</i> Open
Water use efficiency (WUE) is the ratio of biomass gained per unit of water consumed; thus, it can be altered by genetic factors that affect either side of the ratio. In the present study, we exploited natural variation for WUE as an unbia…
View article: Insertion of<i>YFP</i>at<i>P5CS1</i>and<i>AFL1</i>shows the potential, and potential complications, of gene tagging for functional analyses of stress-related proteins
Insertion of<i>YFP</i>at<i>P5CS1</i>and<i>AFL1</i>shows the potential, and potential complications, of gene tagging for functional analyses of stress-related proteins Open
Crispr/CAS9-enabled homologous recombination to insert a tag in frame with an endogenous gene can circumvent difficulties such as context-dependent promoter activity that complicate analysis of gene expression and protein accumulation patt…
View article: Spatial differences in stoichiometry of EGR phosphatase and Microtubule-associated Stress Protein 1 control root meristem activity during drought stress
Spatial differences in stoichiometry of EGR phosphatase and Microtubule-associated Stress Protein 1 control root meristem activity during drought stress Open
During moderate severity drought and low water potential (ψw) stress, poorly understood signaling mechanisms restrict both meristem cell division and subsequent cell expansion. We found that the Arabidopsis thaliana Clade E Growth-Regulati…
View article: Spatial differences in stoichiometry of EGR phosphatase and Microtubule-Associated Stress Protein 1 control root meristem activity during drought stress
Spatial differences in stoichiometry of EGR phosphatase and Microtubule-Associated Stress Protein 1 control root meristem activity during drought stress Open
During moderate severity drought and low water potential (ψ w ) stress, poorly understood signaling mechanisms restrict both meristem cell division and subsequent cell expansion. We found that the Clade E Growth-Regulating 2 ( EGR2 ) prote…
View article: Highly ABA-Induced 1 (HAI1)-Interacting protein HIN1 and drought acclimation-enhanced splicing efficiency at intron retention sites
Highly ABA-Induced 1 (HAI1)-Interacting protein HIN1 and drought acclimation-enhanced splicing efficiency at intron retention sites Open
The Highly ABA-Induced 1 (HAI1) protein phosphatase is a central component of drought-related signaling. A screen for HAI1-interacting proteins identified HAI1-Interactor 1 (HIN1), a nuclear protein of unknown function which could be depho…
View article: The flip side of phospho‐signalling: Regulation of protein dephosphorylation and the protein phosphatase 2Cs
The flip side of phospho‐signalling: Regulation of protein dephosphorylation and the protein phosphatase 2Cs Open
Protein phosphorylation is a key signalling mechanism and has myriad effects on protein function. Phosphorylation by protein kinases can be reversed by protein phosphatases, thus allowing dynamic control of protein phosphorylation. Althoug…
View article: Low Water Potential and At14a-Like1 (AFL1) Effects on Endocytosis and Actin Filament Organization
Low Water Potential and At14a-Like1 (AFL1) Effects on Endocytosis and Actin Filament Organization Open
At14a-Like1 (AFL1) is a stress-induced protein of unknown function that promotes growth during low water potential stress and drought. Previous analysis indicated that AFL1 may have functions related to endocytosis and regulation of actin …
View article: Natural Variation in 9-Cis-Epoxycartenoid Dioxygenase 3 and ABA Accumulation
Natural Variation in 9-Cis-Epoxycartenoid Dioxygenase 3 and ABA Accumulation Open
The stress hormone abscisic acid (ABA) is critical for drought resistance; however, mechanisms controlling ABA levels are unclear. At low water potential, ABA accumulation in the Arabidopsis (Arabidopsis thaliana) accession Shahdara (Sha) …
View article: Phosphoproteomics of <i>Arabidopsis</i> Highly ABA-Induced1 identifies AT-Hook–Like10 phosphorylation required for stress growth regulation
Phosphoproteomics of <i>Arabidopsis</i> Highly ABA-Induced1 identifies AT-Hook–Like10 phosphorylation required for stress growth regulation Open
The clade A protein phosphatase 2C Highly ABA-Induced 1 (HAI1) plays an important role in stress signaling, yet little information is available on HAI1-regulated phosphoproteins. Quantitative phosphoproteomics identified phosphopeptides of…
View article: Natural variation identifies genes affecting drought-induced abscisic acid accumulation in <i>Arabidopsis thaliana</i>
Natural variation identifies genes affecting drought-induced abscisic acid accumulation in <i>Arabidopsis thaliana</i> Open
Significance Abscisic acid (ABA) accumulates to high levels during drought stress, and ABA levels, in turn, control many downstream responses that determine plant growth, productivity, and survival during drought. Despite the central impor…
View article: Comparative Analysis of Phosphoproteome Remodeling After Short Term Water Stress and ABA Treatments versus Longer Term Water Stress Acclimation
Comparative Analysis of Phosphoproteome Remodeling After Short Term Water Stress and ABA Treatments versus Longer Term Water Stress Acclimation Open
Several studies have used short term dehydration, osmotic stress or Abscisic Acid (ABA) treatments to identify the initial protein phosphorylation-dephosphorylation responses to drought and low water potential or ABA treatments. However, l…
View article: Protein Phosphatase 2Cs and <i>Microtubule-Associated Stress Protein 1</i> Control Microtubule Stability, Plant Growth, and Drought Response
Protein Phosphatase 2Cs and <i>Microtubule-Associated Stress Protein 1</i> Control Microtubule Stability, Plant Growth, and Drought Response Open
Plant growth is coordinated with environmental factors, including water availability during times of drought. Microtubules influence cell expansion; however, the mechanisms by which environmental signals impinge upon microtubule organizati…
View article: Interactive effects of water limitation and elevated temperature on the physiology, development and fitness of diverse accessions of <i>Brachypodium distachyon</i>
Interactive effects of water limitation and elevated temperature on the physiology, development and fitness of diverse accessions of <i>Brachypodium distachyon</i> Open
Summary An enduring question in plant physiology and evolution is how single genotypes of plants optimize performance in diverse, often highly variable, environments. We grew 35 natural accessions of the grass Brachypodium distachyon in fo…
View article: Time to grow: factors that control plant growth during mild to moderate drought stress
Time to grow: factors that control plant growth during mild to moderate drought stress Open
This article comments on: Time of day determines Arabidopsis transcriptome and growth dynamics under mild drought
View article: Proline coordination with fatty acid synthesis and redox metabolism of chloroplast and mitochondria
Proline coordination with fatty acid synthesis and redox metabolism of chloroplast and mitochondria Open
Proline (Pro) accumulation is one of the most prominent changes in plant metabolism during drought and low water potential; however, the regulation and function of Pro metabolism remain unclear. We used a combination of forward genetic scr…
View article: At14a-Like1 participates in membrane-associated mechanisms promoting growth during drought in <i>Arabidopsis thaliana</i>
At14a-Like1 participates in membrane-associated mechanisms promoting growth during drought in <i>Arabidopsis thaliana</i> Open
Significance Drought is a major cause of lost agricultural productivity. Even moderate water limitation can lead to down-regulation of plant growth; however, the underlying mechanisms of stress sensing and growth regulation are little unde…
View article: Dynamic proline metabolism: importance and regulation in water limited environments
Dynamic proline metabolism: importance and regulation in water limited environments Open
Drought-induced proline accumulation observed in many plant species has led to the hypothesis that further increases in proline accumulation would promote drought tolerance. Here we discuss both previous and new data showing that proline m…
View article: The ongoing search for the molecular basis of plant osmosensing
The ongoing search for the molecular basis of plant osmosensing Open
Introduction: Cell viability and metabolism depend on cytoplasmic water and solute content, and organisms have evolved mechanisms to sense changes in cell water content, solute concentrations, cell volume, and/or turgor. This Perspective a…
View article: Exploiting Differential Gene Expression and Epistasis to Discover Candidate Genes for Drought-Associated QTLs in <i>Arabidopsis thaliana</i>
Exploiting Differential Gene Expression and Epistasis to Discover Candidate Genes for Drought-Associated QTLs in <i>Arabidopsis thaliana</i> Open
Soil water availability represents one of the most important selective agents for plants in nature and the single greatest abiotic determinant of agricultural productivity, yet the genetic bases of drought acclimation responses remain poor…