Pavel Měřička
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View article: Microbiological risk assessment during the procurement and processing of cells, tissues and breast milk using the MiRCA tool
Microbiological risk assessment during the procurement and processing of cells, tissues and breast milk using the MiRCA tool Open
The Microbiological Risk Classification and Assessment tool (MiRCA) is a valuable instrument elaborated by the European Directorate for the Quality of Medicines & HealthCare (EDQM) for evaluation of the microbiological risks associated wit…
View article: Assessment of DNA damage of smooth muscle cells in tunica media of human arterial allografts using Comet assay method
Assessment of DNA damage of smooth muscle cells in tunica media of human arterial allografts using Comet assay method Open
During the harvest and subsequent processing of blood vessels for allogeneic transplantation, DNA damage can occur. A suitable method for quantifying this damage may be the comet assay, also known as the single cell gel electrophoresis ass…
View article: PERSPECTIVE: Potential impact of the forthcoming european union substances of human origin regulation on the programme of allotransplantation of cryopreserved vascular tissue grafts
PERSPECTIVE: Potential impact of the forthcoming european union substances of human origin regulation on the programme of allotransplantation of cryopreserved vascular tissue grafts Open
The Czech vascular tissue transplantation programme has operated under the conditions set by European Union (EU) directives since 2013. The aim of this analysis was to assess the impact of the new EU regulation on it and to analyse potenti…
View article: Post-thaw dimethyl sulfoxide reduction in autologous peripheral blood progenitor cell suspensions
Post-thaw dimethyl sulfoxide reduction in autologous peripheral blood progenitor cell suspensions Open
The dimethyl sulfoxide removal process and total process recoveries revealed considerable individual variability. To minimize the risk of prolonged engraftment or non-engraftment, it is important to apply this process only to high-risk pat…
View article: Effect of pasteurisation and high-pressure processing on selected bioactive components in human milk - An experimental study
Effect of pasteurisation and high-pressure processing on selected bioactive components in human milk - An experimental study Open
High-pressure processing (HPP) represents a promising alternative to conventional Holder pasteurisation (HoP) used by human milk banks worldwide. The objective of this study was to identify whether the HPP would achieve the same or better …
View article: The Modern Cryobank as Key Support for Clinical Cell and Tissue Transplantation Programmes
The Modern Cryobank as Key Support for Clinical Cell and Tissue Transplantation Programmes Open
The authors review the essential role of modern cryobanks in the development of cryotechnology before the start of the clinical cell and tissue transplantation programme and in the routine support of these programmes within the legislative…
View article: Viability of Human Arterial Grafts Monitored by Comet Assay
Viability of Human Arterial Grafts Monitored by Comet Assay Open
An analytical method for studying DNA degradation by electrophoresis after cell lysis and visualization of DNA fragments with fluorescent dye, comet assay, was used to evaluate the viability of the endothelial layer of human arterial graft…
View article: High-Pressure Inactivation of Bacillus cereus in Human Breast Milk
High-Pressure Inactivation of Bacillus cereus in Human Breast Milk Open
Although Holder pasteurization is the recommended method for processing breast milk, it does affect some of its nutritional and biological properties and is ineffective at inactivating spores. The aim of this study was to find and validate…
View article: Perspective: The Role of Cryopreservation Techniques in Manufacturing, Transport, and Storage of Car-T Therapy Products
Perspective: The Role of Cryopreservation Techniques in Manufacturing, Transport, and Storage of Car-T Therapy Products Open
Several clinical trials have proved the efficacy and safety of T-cells chimeric antigen receptor (CAR-T cells) in treatment of malignant lymphoma and the first products were registered in the European Union in 2018. The shelf-life of CAR-T…
View article: Influence of the Me2SO removal on key parameters of cryopreserved autologous peripheral progenitor cells concentrates: a retrospective study in patients with primary or secondary amyloidosis
Influence of the Me2SO removal on key parameters of cryopreserved autologous peripheral progenitor cells concentrates: a retrospective study in patients with primary or secondary amyloidosis Open
View article: Quantitative Risk Assessment of Bacillus cereus Growth during the Warming of Thawed Pasteurized Human Banked Milk Using a Predictive Mathematical Model
Quantitative Risk Assessment of Bacillus cereus Growth during the Warming of Thawed Pasteurized Human Banked Milk Using a Predictive Mathematical Model Open
Bacillus cereus is relatively resistant to pasteurization. We assessed the risk of B. cereus growth during warming and subsequent storage of pasteurized banked milk (PBM) in the warmed state using a predictive mathematical model. Holder pa…
View article: Bacillus cereus as a Major Cause of Discarded Pasteurized Human Banked Milk: A Single Human Milk Bank Experience
Bacillus cereus as a Major Cause of Discarded Pasteurized Human Banked Milk: A Single Human Milk Bank Experience Open
A systematic study, performed from 2017–2020 looked at the rate of positive post-pasteurization B. cereus findings, the quantity of B. cereus in pasteurized banked human milk (PBM), and the rate of B. cereus toxicogenic isolates from PBM. …
View article: Cell Viability Assessment Using Fluorescence Vital Dyes and Confocal Microscopy in Evaluating Freezing and Thawing Protocols Used in Cryopreservation of Allogeneic Venous Grafts
Cell Viability Assessment Using Fluorescence Vital Dyes and Confocal Microscopy in Evaluating Freezing and Thawing Protocols Used in Cryopreservation of Allogeneic Venous Grafts Open
The authors present their contribution to the improvement of methods suitable for the detection of the freezing and thawing damage of cells of cryopreserved venous grafts used for lower limb revascularization procedures. They studied the p…
View article: Influence of the new standardized clinical cryopreservation/slow thawing protocol on immunogenicity of arterial allografts in rats
Influence of the new standardized clinical cryopreservation/slow thawing protocol on immunogenicity of arterial allografts in rats Open
Aortal wall histology of rat allografts treated by our new standardized clinical cryopreservation/slow thawing protocol was comparable to that of the cryopreserved isografts on day 30 posttranspant. The immunogenicity of cryopreserved aort…
View article: Dimethyl Sulfoxide: A Central Player Since the Dawn of Cryobiology, is Efficacy Balanced by Toxicity?
Dimethyl Sulfoxide: A Central Player Since the Dawn of Cryobiology, is Efficacy Balanced by Toxicity? Open
Dimethyl sulfoxide (DMSO) is the cryoprotectant of choice for most animal cell systems since the early history of cryopreservation. It has been used for decades in many thousands of cell transplants. These treatments would not have taken p…
View article: The Effect of Different Thawing Rates on Cryopreserved Human Iliac Arteries Allograft’s Structural Damage and Mechanical Properties
The Effect of Different Thawing Rates on Cryopreserved Human Iliac Arteries Allograft’s Structural Damage and Mechanical Properties Open
Introduction . The rate of thawing of cryopreserved human iliac arteries allografts (CHIAA) directly affects the severeness of structural changes that occur during this process. Method . The experiment was performed on ten CHIAA. The 10% d…
View article: Current vascular allograft procurement, cryopreservationand transplantation techniques in the Czech Republic
Current vascular allograft procurement, cryopreservationand transplantation techniques in the Czech Republic Open
There are several crucial moments affecting final quality, including graft retrieval within a multiorgan harvest, short ischemic time, cryopreservation and thawing technique used. The recommended surgical handling during implantation may a…
View article: Own experience from the 22 Year lasting process of harmonization of the European Union safety and quality requirements forharvesting processing and distribution of human cells and tissues used for clinical transplantation.
Own experience from the 22 Year lasting process of harmonization of the European Union safety and quality requirements forharvesting processing and distribution of human cells and tissues used for clinical transplantation. Open
In Europe the issue of regulation of safety and quality of tissue grafts was first discussed at the EATB meeting in Leuven in 1995. In the years 2004 and 2006 the pertinent Directives of the European Parliament and Council and of the Europ…
View article: Comparative Study on the Application of Mesenchymal Stromal Cells Combined with Tricalcium Phosphate Scaffold into Femoral Bone Defects
Comparative Study on the Application of Mesenchymal Stromal Cells Combined with Tricalcium Phosphate Scaffold into Femoral Bone Defects Open
This prospective study sought to evaluate the healing quality of implanted ultraporous β-tricalcium phosphate sown with expanded autologous mesenchymal stromal cells (MSCs) into femoral defects during revision hip arthroplasty. A total of …
View article: Structural changes arising from different thawing protocols on cryopreserved human allograft’s aortic valve leaflets
Structural changes arising from different thawing protocols on cryopreserved human allograft’s aortic valve leaflets Open
Based on our experimental work, we can clearly conclude that cryopreserved AV leaflet allografts show identical structural changes at different rates of thawing.
View article: Immunosuppressive protocols with tacrolimus after cryopreserved aortal allotransplantation in rats
Immunosuppressive protocols with tacrolimus after cryopreserved aortal allotransplantation in rats Open
Both immunosuppressed protocols with tacrolimus (administration from day 1 or from day 7 following transplantation) were able to suppress acute cell- and antibody-mediated rejection of cryopreserved abdominal aortic allografts processed in…
View article: Elution kinetics of vancomycin and gentamicin from carriers and their effects on mesenchymal stem cell proliferation: an in vitro study
Elution kinetics of vancomycin and gentamicin from carriers and their effects on mesenchymal stem cell proliferation: an in vitro study Open
This study demonstrated the benefits of antibiotic impregnated cancellous allogeneic bone grafts versus other carriers.
View article: Composite venous allograft for femoro-pedal bypass grafting in critical limb ischaemia
Composite venous allograft for femoro-pedal bypass grafting in critical limb ischaemia Open
Základní informace: Popisujeme soubor čtyř pacientů s kritickou ischemií končetiny a absencí autologních náhrad (konduitů), kteří byli ošetřeni vytvořením kompozitního (s anastomózou "end-to-end") alogenního bypassu z čerstvého nebo kryopr…
View article: Cryopreserved human aortic root allografts arterial wall: Structural changes occurring during thawing
Cryopreserved human aortic root allografts arterial wall: Structural changes occurring during thawing Open
All the samples thawed at the room temperature showed smaller structural damage to the CHARA arterial wall with no smooth muscle cell contraction in tunica media when compared to the samples thawed in a water bath.
View article: How long do we need to store cryopreserved cells and tissues.
How long do we need to store cryopreserved cells and tissues. Open
Cryopreservation of haematopoietic tissue used in autologous and allogeneic transplantation was introduced to clinical practice at the author`s workplace in the 80´s and 90´s of the last century. Later cryopreservation of veins and arterie…
View article: Long-term experience with operation of a cryobank based on combination of cryogenic and clean-room technology.
Long-term experience with operation of a cryobank based on combination of cryogenic and clean-room technology. Open
The modern cryobank equipped with large liquid nitrogen (LN2) containers for long term storage of human cells and tissues for clinical application and programmable freezers for controlled rate freezing of cells and tissues prior to storage…