Payam E. Farahani
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View article: <i>In vivo</i> measurements of receptor tyrosine kinase activity reveal feedback regulation of a developmental gradient
<i>In vivo</i> measurements of receptor tyrosine kinase activity reveal feedback regulation of a developmental gradient Open
A lack of tools for detecting receptor activity in vivo has limited our ability to fully explore receptor-level control of developmental patterning. Here, we extend a new class of biosensors for receptor tyrosine kinase (RTK) activity, the…
View article: CD44 and Ezrin restrict EGF receptor mobility to generate a novel spatial arrangement of cytoskeletal signaling modules driving bleb-based migration
CD44 and Ezrin restrict EGF receptor mobility to generate a novel spatial arrangement of cytoskeletal signaling modules driving bleb-based migration Open
Cells under high confinement form highly polarized hydrostatic pressure-driven, stable leader blebs that enable efficient migration in low adhesion, environments. Here we investigated the basis of the polarized bleb morphology of metastati…
View article: Large-scale control over collective cell migration using light-controlled epidermal growth factor receptors
Large-scale control over collective cell migration using light-controlled epidermal growth factor receptors Open
Receptor tyrosine kinases (RTKs) are thought to play key roles in coordinating cell movement at single-cell and tissue scales. The recent development of optogenetic tools for controlling RTKs and their downstream signaling pathways suggest…
View article: pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells
pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells Open
Receptor tyrosine kinases (RTKs) are major signaling hubs in metazoans, playing crucial roles in cell proliferation, migration, and differentiation. However, few tools are available to measure the activity of a specific RTK in individual l…
View article: Author response: pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells
Author response: pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells Open
Full text Figures and data Side by side Abstract Editor's evaluation Introduction Results Discussion Methods Appendix 1 Data availability References Decision letter Author response Article and author information Metrics Abstract Receptor t…
View article: pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells
pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells Open
Receptor tyrosine kinases (RTKs) are major signaling hubs in metazoans, playing crucial roles in cell proliferation, migration, and differentiation. However, few tools are available to measure the activity of a specific RTK in individual l…
View article: Substratum stiffness regulates Erk signaling dynamics through receptor-level control
Substratum stiffness regulates Erk signaling dynamics through receptor-level control Open
The EGFR/Erk pathway is triggered by extracellular ligand stimulation, leading to stimulus-dependent dynamics of pathway activity. Although mechanical properties of the microenvironment also affect Erk activity, their effects on Erk signal…
View article: Signaling, Deconstructed: Using Optogenetics to Dissect and Direct Information Flow in Biological Systems
Signaling, Deconstructed: Using Optogenetics to Dissect and Direct Information Flow in Biological Systems Open
Cells receive enormous amounts of information from their environment. How they act on this information—by migrating, expressing genes, or relaying signals to other cells—comprises much of the regulatory and self-organizational complexity f…
View article: Purification, kinetic characterization, and site-directed mutagenesis of <em>Methanothermobacter thermautotrophicus</em> RFAP Synthase Produced in <em>Escherichia coli</em>
Purification, kinetic characterization, and site-directed mutagenesis of <em>Methanothermobacter thermautotrophicus</em> RFAP Synthase Produced in <em>Escherichia coli</em> Open
Methane-producing archaea are among a select group of microorganisms that utilize tetrahydromethanopterin (H4MPT) as a one-carbon carrier instead of tetrahydrofolate. In H4MPT biosynthesis, β-ribofuranosylaminobenzene 5'-phosphate (RFAP) s…
View article: Light-Activated Proteomic Labeling <i>via</i> Photocaged Bioorthogonal Non-Canonical Amino Acids
Light-Activated Proteomic Labeling <i>via</i> Photocaged Bioorthogonal Non-Canonical Amino Acids Open
This work introduces light-activated bioorthogonal noncanonical amino acid tagging (laBONCAT) as a method to selectively label, isolate, and identify proteins newly synthesized at user-defined regions in tissue culture. By photocaging l-az…
View article: Photomediated oxime ligation as a bioorthogonal tool for spatiotemporally-controlled hydrogel formation and modification
Photomediated oxime ligation as a bioorthogonal tool for spatiotemporally-controlled hydrogel formation and modification Open
Biocompatible, synthetic hydrogels were formed and modified by photomediated oxime ligation with full spatiotemporal control.