Pedro Antas
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View article: Loss of REP-1 in retinal pigment epithelial cells leads to impaired phagosome processing and altered lysosomal pathway function
Loss of REP-1 in retinal pigment epithelial cells leads to impaired phagosome processing and altered lysosomal pathway function Open
Choroideremia (CHM) is a rare form of retinal degeneration caused by mutations in the ubiquitously expressed CHM gene, encoding for Rab escort-protein-1 (REP-1). REP-1 is required for the prenylation of Rab GTPase proteins, regulators of i…
View article: Enhancing lysosome function via mTOR/TFEB activation reduces lipofuscin-like granules in early Age-related Macular Degeneration
Enhancing lysosome function via mTOR/TFEB activation reduces lipofuscin-like granules in early Age-related Macular Degeneration Open
Age-related macular degeneration (AMD) is the most common blinding disease in the western world and is currently incurable. Although the exact causes of AMD are not clear, the primary origin of pathology appears to be in the retinal pigmen…
View article: Modeling Choroideremia Disease with Isogenic Induced Pluripotent Stem Cells
Modeling Choroideremia Disease with Isogenic Induced Pluripotent Stem Cells Open
Choroideremia (CHM) is a rare X-linked chorioretinal dystrophy causing progressive vision loss due to mutations in the CHM gene, leading to Rab escort protein 1 loss of function. CHM disease is characterized by a progressive degeneration o…
View article: Modelling neurodegeneration and inflammation in early diabetic retinopathy using 3D human retinal organoids
Modelling neurodegeneration and inflammation in early diabetic retinopathy using 3D human retinal organoids Open
Purpose Diabetic retinopathy (DR) is a complication of diabetes and a primary cause of visual impairment amongst working-age individuals. DR is a degenerative condition in which hyperglycaemia results in morphological and functional change…
View article: Gene therapy for inherited retinal diseases: exploiting new tools in genome editing and nanotechnology
Gene therapy for inherited retinal diseases: exploiting new tools in genome editing and nanotechnology Open
Inherited retinal diseases (IRDs) encompass a diverse group of genetic disorders that lead to progressive visual impairment and blindness. Over the years, considerable strides have been made in understanding the underlying molecular mechan…
View article: 3D human retinal organoid model for the study of early diabetic retinopathy
3D human retinal organoid model for the study of early diabetic retinopathy Open
Diabetic retinopathy (DR) is a significant complication of diabetes and a primary cause of visual impairment among working-age individuals. DR is a degenerative condition in which hyperglycaemia results in morphological and functional chan…
Impaired Lysosome Reformation in Chloroquine-Treated Retinal Pigment Epithelial Cells Open
Low-dose chloroquine treatment inhibits lysosome reformation, causing a gradual depletion of lysosomes able to interact with cargo-carrying vacuoles and degrade their content. The resulting accumulation of newly synthesized pro-cathepsin D…
Formation of Lipofuscin-Like Autofluorescent Granules in the Retinal Pigment Epithelium Requires Lysosome Dysfunction Open
We conclude that lysosomal dysfunction results in incomplete POS degradation and enhanced AFG accumulation.
FORMATION OF LIPOFUSCIN-LIKE AUTOFLUORESCENT GRANULES IN THE RETINAL PIGMENT EPITHELIUM REQUIRES LYSOSOME DYSFUNCTION Open
Purpose We aim to characterize the pathways required for autofluorescent granule (AFG) formation by retinal pigment epithelium (RPE) cells using cultured monolayers. Methods We fed RPE monolayers in culture with a single pulse of photorece…
View article: Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)<sup>1</sup>
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)<sup>1</sup> Open
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologi…
The synthetic cannabinoid JWH-018 modulates <i>Saccharomyces cerevisiae</i> energetic metabolism Open
Synthetic cannabinoids are a group of novel psychoactive substances with similar properties to Δ9-THC. Among the vast number of synthetic cannabinoids, designed to be tested in clinical trials, JWH-018 was the first novel psychoactive subs…
USP7 Is a Tumor-Specific WNT Activator for APC-Mutated Colorectal Cancer by Mediating β-Catenin Deubiquitination Open
The tumor suppressor gene adenomatous polyposis coli (APC) is mutated in most colorectal cancers (CRCs), resulting in constitutive Wnt activation. To understand the Wnt-activating mechanism of the APC mutation, we applied CRISPR/Cas9 techn…
Correction: The mechanism of sirtuin 2-mediated exacerbation of alpha-synuclein toxicity in models of Parkinson disease Open
[This corrects the article DOI: 10.1371/journal.pbio.2000374.].
The mechanism of sirtuin 2–mediated exacerbation of alpha-synuclein toxicity in models of Parkinson disease Open
Sirtuin genes have been associated with aging and are known to affect multiple cellular pathways. Sirtuin 2 was previously shown to modulate proteotoxicity associated with age-associated neurodegenerative disorders such as Alzheimer and Pa…