Peter Kristensen
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View article: Investigation of fouling in perfusion cell culture processes using alternating tangential flow filtration
Investigation of fouling in perfusion cell culture processes using alternating tangential flow filtration Open
Microfiltration membranes are increasingly used to retain the cells inside bioreactors while continuous harvest of the desired biopharmaceutical occurs in perfusion processes. One method of microfiltration is Alternating Tangential Flow (A…
View article: Design and engineering of bispecific antibodies: insights and practical considerations
Design and engineering of bispecific antibodies: insights and practical considerations Open
Bispecific antibodies (bsAbs) have attracted significant attention due to their dual binding activity, which permits simultaneous targeting of antigens and synergistic binding effects beyond what can be obtained even with combinations of c…
View article: Structural trends in antibody-antigen binding interfaces: a computational analysis of 1833 experimentally determined 3D structures
Structural trends in antibody-antigen binding interfaces: a computational analysis of 1833 experimentally determined 3D structures Open
Antibodies are attractive therapeutic candidates due to their ability to bind cognate antigens with high affinity and specificity. Still, the underlying molecular rules governing the antibody-antigen interface remain poorly understood, mak…
View article: A window into the human immune system: comprehensive characterization of the complexity of antibody complementary-determining regions in functional antibodies
A window into the human immune system: comprehensive characterization of the complexity of antibody complementary-determining regions in functional antibodies Open
The human immune system uses antibodies to neutralize foreign antigens. They are composed of heavy and light chains, both with constant and variable regions. The variable region has six hypervariable loops, also known as complementary-dete…
View article: Erratum to: Patellofemoral Arthroplasty Results in Better Time-weighted Patient-reported Outcomes After 6 Years than TKA: A Randomized Controlled Trial
Erratum to: Patellofemoral Arthroplasty Results in Better Time-weighted Patient-reported Outcomes After 6 Years than TKA: A Randomized Controlled Trial Open
In the study, “Patellofemoral Arthroplasty Results in Better Time-weighted Patient-reported Outcomes After 6 Years than TKA: A Randomized Controlled Trial,” the authors uncovered an error in the algorithm calculating areas under the curve …
View article: Generation of robust bispecific antibodies through fusion of single-domain antibodies on IgG scaffolds: a comprehensive comparison of formats
Generation of robust bispecific antibodies through fusion of single-domain antibodies on IgG scaffolds: a comprehensive comparison of formats Open
Bispecific antibodies (bsAbs) enable dual binding of different antigens with potential synergistic targeting effects and innovative therapeutic possibilities. The formation of bsAbs is, however, often dependent on complex engineering strat…
View article: Eliminating OFF-frame clones in randomized gene libraries: An improved split β-lactamase enrichment system
Eliminating OFF-frame clones in randomized gene libraries: An improved split β-lactamase enrichment system Open
Large, randomized libraries are a key technology for many biotechnological applications. While genetic diversity is the main parameter most libraries direct their resources on, less focus is devoted to ensuring functional IN-frame expressi…
View article: VH-Based Mini Q-Body: A Novel Quench-Based Immunosensor
VH-Based Mini Q-Body: A Novel Quench-Based Immunosensor Open
Quenchbodies (Q-bodies), a type of biosensor, are antibodies labeled with a fluorescent dye near the antigen recognition site. In the absence of an antigen, the dye is quenched by tryptophans in the antibody sequence; however, in its prese…
View article: Immobilization-Free Binding and Affinity Characterization of Higher Order Bispecific Antibody Complexes Using Size-Based Microfluidics
Immobilization-Free Binding and Affinity Characterization of Higher Order Bispecific Antibody Complexes Using Size-Based Microfluidics Open
Simultaneous targeting of different antigens by bispecific antibodies (bsAbs) is permitting synergistic binding functionalities with high therapeutic potential, but is also rendering their analysis challenging. We introduce flow-induced di…
View article: Topoisomerase 1 inhibits <i>MYC</i> promoter activity by inducing G-quadruplex formation
Topoisomerase 1 inhibits <i>MYC</i> promoter activity by inducing G-quadruplex formation Open
We have investigated the function of human topoisomerase 1 (TOP1) in regulation of G-quadruplex (G4) formation in the Pu27 region of the MYC P1 promoter. Pu27 is among the best characterized G4 forming sequences in the human genome and it …
View article: Restrictive fluids versus standard care in adults with sepsis in the emergency department (<scp>REFACED</scp>): A multicenter, randomized feasibility trial
Restrictive fluids versus standard care in adults with sepsis in the emergency department (<span>REFACED</span>): A multicenter, randomized feasibility trial Open
Background Fluid treatment in sepsis is a challenge and clinical equipoise exists regarding intravenous (IV) volumes. We aimed to determine whether a 24‐h protocol restricting IV fluid was feasible in adult patients with sepsis without sho…
View article: Differential effects of cryo-concentration on volatiles in apple juice
Differential effects of cryo-concentration on volatiles in apple juice Open
Cryo-concentration of apple juice shows that concentration of esters increases proportionally with increasing Brix value while the concentration of furan compounds has an irregular but in some cases close to exponential increase during the…
View article: Multiple stressors determine river ecological status at the European scale: Towards an integrated understanding of river status deterioration
Multiple stressors determine river ecological status at the European scale: Towards an integrated understanding of river status deterioration Open
The biota of European rivers are affected by a wide range of stressors impairing water quality and hydro‐morphology. Only about 40% of Europe's rivers reach ‘good ecological status’, a target set by the European Water Framework Directive (…
View article: Dataset on Article: Multiple stressors determine river ecological status at the European scale: Towards an integrated understanding of river status deterioration
Dataset on Article: Multiple stressors determine river ecological status at the European scale: Towards an integrated understanding of river status deterioration Open
The shape file contains the raw data from the Global Change Biology article "River types, hydrology, riparian land use, nutrients, toxic substances, ecological status, stressor interactions". General Information Name of the dataset: Multip…
View article: Dataset on Article: Multiple stressors determine river ecological status at the European scale: Towards an integrated understanding of river status deterioration
Dataset on Article: Multiple stressors determine river ecological status at the European scale: Towards an integrated understanding of river status deterioration Open
The shape file contains the raw data from the Global Change Biology article "River types, hydrology, riparian land use, nutrients, toxic substances, ecological status, stressor interactions". General Information Name of the dataset: Multip…
View article: Moving Toward an Agenda on Ocean Health and Human Health in Europe
Moving Toward an Agenda on Ocean Health and Human Health in Europe Open
This manuscript is the result of an AZTI’s Summer School, celebrated in San Sebastian (Spain), from 5th to 7th June, 2019.-- 19 pages, 3 figures, 2 tables, 1 box
View article: Rational Engineering of Hydratase from <i>Lactobacillus acidophilus</i> Reveals Critical Residues Directing Substrate Specificity and Regioselectivity
Rational Engineering of Hydratase from <i>Lactobacillus acidophilus</i> Reveals Critical Residues Directing Substrate Specificity and Regioselectivity Open
Enzymatic conversion of fatty acids (FAs) by fatty acid hydratases (FAHs) presents a green and efficient route for high‐value hydroxy fatty acid (HFA) production. However, limited diversity was achieved among HFAs, to date, with respect to…
View article: Rational Engineering of Hydratase from Lactobacillus Acidophilus Reveals Critical Residues Directing Substrate Specificity and Regioselectivity
Rational Engineering of Hydratase from Lactobacillus Acidophilus Reveals Critical Residues Directing Substrate Specificity and Regioselectivity Open
Enzymatic conversion of abundant fatty acids (FAs) through fatty acid hydratases (FAHs) presents an environment-friendly and efficient route for production of high-value hydroxy fatty acids (HFAs). However, a limited diversity was achieved…
View article: Rational Engineering of Hydratase from Lactobacillus Acidophilus Reveals Critical Residues Directing Substrate Specificity and Regioselectivity
Rational Engineering of Hydratase from Lactobacillus Acidophilus Reveals Critical Residues Directing Substrate Specificity and Regioselectivity Open
Enzymatic conversion of abundant fatty acids (FAs) through fatty acid hydratases (FAHs) presents an environment-friendly and efficient route for production of high-value hydroxy fatty acids (HFAs). However, a limited diversity was achieved…
View article: Rational Engineering of Hydratase from Lactobacillus Acidophilus Reveals Critical Residues Directing Substrate Specificity and Regioselectivity
Rational Engineering of Hydratase from Lactobacillus Acidophilus Reveals Critical Residues Directing Substrate Specificity and Regioselectivity Open
Enzymatic conversion of abundant fatty acids (FAs) through fatty acid hydratases (FAHs) presents an environment-friendly and efficient route for production of high-value hydroxy fatty acids (HFAs). However, a limited diversity was achieved…
View article: Insight into the Structure and Activity of Surface‐Engineered Lipase Biofluids
Insight into the Structure and Activity of Surface‐Engineered Lipase Biofluids Open
Despite a successful application of solvent‐free liquid protein (biofluids) concept to a number of commercial enzymes, the technical advantages of enzyme biofluids as hyperthermal stable biocatalysts cannot be fully utilized as up to 90–99…
View article: Similar polyethylene wear between cemented and cementless Oxford medial UKA: a 5-year follow-up randomized controlled trial on 79 patients using radiostereometry
Similar polyethylene wear between cemented and cementless Oxford medial UKA: a 5-year follow-up randomized controlled trial on 79 patients using radiostereometry Open
Background and purpose — Hydroxyapatite (HA)-coated implants have been associated with high polyethylene wear in hip arthroplasties. HA coating as a promoter of wear in knee arthroplasties has not been investigated. We compared the wear-ra…